首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   122篇
  免费   5篇
林业   7篇
农学   17篇
  17篇
综合类   4篇
农作物   10篇
水产渔业   12篇
畜牧兽医   49篇
园艺   1篇
植物保护   10篇
  2023年   1篇
  2022年   3篇
  2021年   4篇
  2020年   6篇
  2019年   4篇
  2018年   5篇
  2017年   3篇
  2016年   11篇
  2015年   2篇
  2014年   1篇
  2013年   9篇
  2012年   8篇
  2011年   7篇
  2010年   8篇
  2009年   3篇
  2008年   9篇
  2007年   5篇
  2006年   6篇
  2005年   4篇
  2004年   3篇
  2003年   3篇
  2002年   5篇
  2001年   5篇
  2000年   1篇
  1999年   5篇
  1998年   1篇
  1994年   1篇
  1990年   1篇
  1989年   1篇
  1987年   1篇
  1973年   1篇
排序方式: 共有127条查询结果,搜索用时 171 毫秒
121.
The compatibility and growth performance of silver barb Puntius gonionotus (Barbonymous gonionotus) with the three Indian major carps, i.e., Catla catla, Labeo rohita and Cirrhinus mrigala, were assessed in a 10‐month carp polyculture trial. Treatments T‐1, T‐2, T‐3 and T‐4 were stocked with three of the above four carp species, with an absence of silver barb, mrigal, rohu and catla, respectively, while all four species were stocked in treatment T‐5. The treatments were stocked at 6000 fingerlings ha−1, with an equal species ratio maintained in each treatment. Incorporation of silver barb into the polyculture system neither affected the survival of any carp irrespective of species combination nor yielded significant changes in biomass production among treatments, except for the one without catla, where it was significantly low. The study revealed a higher extent of competition between silver barb and rohu, perceptible from the lower growth of one in the presence of the other. Although a certain level of competition of silver barb with mrigal was evident, competition with catla was not perceptible. Irrespective of species combination with silver barb as a component species, similar total biomass production in treatments revealed the feasibility of its incorporation into the Indian major carp‐based polyculture practice without affecting the total yield.  相似文献   
122.
The plant, Salvinia natans L., was found to be very useful in the removal of Cu (II) from waste water. Maximum accumulation was noted within one day and maximum removal (about 90%) was recorded below 50 μg mL?1 of Cu (II). Copper was successfully removed from the waste water of Hindusthan Copper Limited (HCL), ICC, Ghatsila, Bihar, India.  相似文献   
123.
The present study evaluated the effect of supplementation of retinol in the vitrification solution on the viability, apoptosis and development-related gene expression in vitrified buffalo preantral follicles. Preantral follicles isolated from cortical slices of ovaries were randomly assigned into three groups: Group1—Control fresh preantral follicles; Group 2—Vitrification treatment (Vitrification solution 1 (VS1) –TCM-199 + 25 mM HEPES + Foetal bovine serum (FBS) 10%, Ethylene glycol (EG): 10%, Dimethyl sulphoxide (DMSO): 10%, Sucrose-0.3 M for 4 min; VS2- TCM-199 + 25 mM HEPES + FBS10%, EG:25%, DMSO: 25%, Sucrose:0.3 M for 45 s); Group3—vitrification treatment +5 μM of Retinol. Preantral follicles were placed in corresponding vitrification medium and plunged into liquid nitrogen (−196°C). After a week, the follicles were thawed and analysed for follicular viability and gene expression. There was no significant difference in the viability rates among the Group 1(Fresh preantral follicles) (91.46 ± 2.39%), Group 2 (89.59 ± 2.46%) and Group 3 (87.19 ± 4.05%). There was a significantly (p < .05) higher mRNA expression of BCL2L1, GDF-9 and BMP-15 in the vitrification + retinol group compared with the control group. There was a significantly (p < .05) higher expression of Caspase-3 and Annexin-5 in the vitrification group and Vitrification + retinol group compared with control group of follicles. It is concluded that the supplementation of 5 μM of Retinol in Vitrification solution was an efficient vitrification procedure for the vitrification of buffalo preantral follicles.  相似文献   
124.
Spring wheat is the second option of cereal crop to feed the growing population in Bangladesh. This crop can yield more than 5.0 ton ha-1 with optimum management on 6.1 million ha of land - 2.6 million ha with irrigation and 3.5 million ha without irrigation. However, the present average wheat yield is near 1.5 ton ha-1, and only 10% of the potential area are under wheat cultivation for various reasons.  相似文献   
125.
In this study, we developed a simple, low-cost, and rapid flow through immunogold assay (FIA) for detection of Aeromonas hydrophila in fish tissues and validated. The developed assay relied on colloidal gold conjugated highly specific monoclonal antibody (B8E3) against 20 kDa protein of A. hydrophila. The assay can be completed within 5 min including antigen (sample) preparation and exhibited no cross-reaction with other major aquatic pathogens such as Vibrio anguillarum, V. parahaemolyticus, V. harveyi, white spot syndrome virus (WSSV), and Aphanomyces invadans. A wider range of Aeromonas species were tested including A. caviae, A. dhakensis, A. sobria, A. veronii, A. culicicola, A. sharmana, and A. schubertii and no cross-reactivity could be observed. The developed technique FIA could detect 103 CFU/ml of aeromonad cells. In addition, the FIA is as sensitive as the first-step polymerase chain reaction (PCR) and 1000 times sensitive than the immunodot blot method. The validation study revealed the accuracy of FIA by comparing with the standard first-step PCR. The proposed FIA is low-cost, easy-to-use, and compatible for field-level analysis of A. hydrophila in fish tissues. Our developed FIA could successfully detect A. hydrophila at early stage of the infection and could be easily availed by farmers, fish producers, non-technicians, and technicians in the aquaculture industry.  相似文献   
126.
Embryonic mortality is found to be the main source of reproductive wastage in domestic ruminants. Many genes are involved in the growth and development of the embryo, and the interferon‐stimulated gene 15 (ISG 15) is one of the major gene stimulated by interferon tau, the maternal recognition of pregnancy signal in ruminants. In this study, both genomic and cDNA sequences of ISG 15 from Bos indicus (Deoni breed) were amplified and characterized. The genomic sequence of Deoni ISG 15 exhibited 99% identity with Bos taurus and 97% identity with that of Bos mutus and Bubalus bubalis. Moreover qRT‐PCR analysis revealed constitutive expression of the ISG 15 mRNA in peripheral blood mononuclear cells of Deoni heifers and multiparous cows during early pregnancy. Fourteen Deoni heifers and fifteen multiparous Deoni cows were synchronized for timed AI by CIDR‐Ovsynch protocol, and six animals were kept as cyclic control in each group. Blood samples were collected on days 7, 14, 16, 18, 21, 30 and 45 from the day of AI. Pregnancy was confirmed by plasma progesterone level through ELISA. A significantly higher expression of ISG 15 mRNA was found on day 16 (< .05) and day 18 (< .05) of pregnancy in nulliparous heifers. Although in multiparous Deoni cows ISG 15 expression was greater in pregnant cows, difference was statistically non‐significant. The result of this study indicates that ISG 15 gene expression is upregulated during 16–18 days of pregnancy and could be used as an early pregnancy marker in dairy cows especially in heifers.  相似文献   
127.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号