Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV   总被引：65，自引：0，他引：65  

F di Marzo Veronese  T D Copeland  A L DeVico  R Rahman  S Oroszlan  R C Gallo  M G Sarngadharan 《Science (New York, N.Y.)》1986,231(4743):1289-1291

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Impact of calcium chloride concentrations and storage duration on quality attributes of peach (<Emphasis Type="Italic">Prunus persica</Emphasis>)     

Mati Ur Rahman  Muhammad Sajid  Abdur Rab  Shahzad Ali  Muhammad Owais Shahid  Aftab Alam  Muhammad Israr  Irshad Ahmad 《Russian Agricultural Sciences》2016,42(2):130-136

To investigate the impact of CaCl₂ concentrations and storage duration on quality of peach (Prunus persica), a research was conducted at postharvest Laboratory, Department of Horticulture, The University of Agriculture, Peshawar, Pakistan during 2012–2013. The experiment was laid out in Completely Randomized Design (CRD) with factorial arrangement repeated three times. The peach fruits (cv. Texas A 69) were harvested at physiological maturity stage from peach orchard, Horticulture Farm. The fruits were dipped in 0, 2 and 4% CaCl₂ solution for 10 min and transferred to cold storage having ±8–10°C with relative humidity of 80–85%. The application of CaCl₂ solution and storage duration significantly influenced the fruit quality of peach fruit. However, the application of CaCl₂ solution significantly reduced weight loss (4.98%), disease incidence (2.08%), total sugars (5.31%), TSS-Acid ratio (16.27), TSS(7.38⁰Brix) and increased the fruit firmness (2.21 kg cm^–2) titratable acidity (0.47%) and Ascorbic acid (5.35 mg/100 g) of peach fruits. The storage duration of peach fruit also significantly affected the fruit quality attributes during storage. The peach fruit stored for 30 days showed less fruit firmness (0.74 kg cm^–2) and titratable acidity (0.31%), Ascorbic acid (4.45 mg/100 g) and increased weight loss (19.74%), disease incidence (16.11%), total sugars (6.07%), TSSAcid ratio (27.62) and TSS(8.54⁰Brix) of peach fruit. Based on the findings of this study, it is concluded that the peach fruit should be treated with 4% CaCl₂ solution to retain the quality attributes for 30 days storage.  相似文献   

Structural and physical properties ofAntheraea pernyi silk fibroin fiber treated with I2-KI aqueous solution     

Md. Majibur Rahman Khan  Yasuo Gotoh  Hideaki Morikawa  Mikihiko Miura 《Fibers and Polymers》2006,7(4):333-338

Silk fibroin (SF) fiber from theAntheraea pernyi silkworm was treated with a 1.23 N iodine-potassium iodide (I₂-KI) aqueous solution, and the structure and physical properties were investigated to clarify the effects of the iodine treatment. The noticeably high weight gain value of SF fiber, about 25 wt% was attributed to the absorption of polyiodide ions in the form of I₃ ^? and I₅ ^?. Fourier transform infrared spectroscopy and X-ray diffraction measurements suggested that polyiodide ions mainly entered the amorphous region. In addition, a new sharp reflection on the meridional direction, corresponding to a period of 7.0 Å, was observed and indicated the possibility of the formation of mesophase structure ofβ-conformation chains. Dynamic viscoelastic measurements showed that the damping tanδ peak at 270°C gradually shifted to lower temperature in the iodinated SF fibers, suggesting an enhancement of the molecular motion of the fibroin chains induced by the presence of polyiodide ions. With heating above 254 °C, the iodine component introduced intermolecular cross-linking of SF, and the melt flow of the sample was inhibited. The thermal decomposition stability of fibroin molecules was greatly enhanced by iodine treatment.  相似文献   

Respiratory bacterial flora from healthy as well as respiratory symptoms' subjects     

Islam M  Hussin S  Rahman MM 《Pakistan journal of biological sciences: PJBS》2011,14(7):456-460

Respiratory system is the primary settlement place of opportunistic organisms and considered as chief carrier of common respiratory pathogens. The aim of the study was to know the opportunistic organisms present in the healthy subjects as well as subjects that were suffering from respiratory symptoms. The organisms were identified as per standard bacteriological protocol and pathogenicity tests of the identified organisms were performed in mouse model. Antibiotic sensitivity of the identified organisms was performed. The bacterial flora present in the throat swab of apparently healthy as well as subjects suffering from respiratory symptoms were: Staphylococcus spp. (39.44%) of which Coagulase positive Staphylococcus (21.13%) and Coagulase negative Staphylococcus (18.31%), Klebsiella spp. (19.72%), Pseudomonas spp. (15.49%), Proteus spp. (4.23%), E. coli (9.86%) and Bacillus spp. (11.27%). Among the isolates Staphylococcus, Klebsiella and Pseudomonas were the predominant species. Percentages of identified bacteria were higher in respiratory symptoms exhibiting individuals (53.52%) than apparently healthy individuals (46.48%). All coagulase positive Staphylococcus, Klebsiella spp. and Pseudomonas spp. isolated from respiratory symptoms' subjects were found to be pathogenic. The isolated bacteria were resistant to amoxicillin and ampicillin but sensitive to ciprofloxacin and norfloxacin. Isolated Pseudomonas spp. showed multidrugs resistant properties. The study provided information about the pathogenic organisms' present respiratory systems of apparently healthy as well as subjects suffering from respiratory symptoms. The pathogenic natures of the isolated organisms were determined to make aware of scientists as well as clinicians. Antibiotics sensitivity assays would provide information to the clinicians for the selection of appropriate antibiotics to treat their patients.  相似文献   

Metabolic pathway of cyanidin 3-O-beta-D-glucopyranoside in rats     

Ichiyanagi T  Shida Y  Rahman MM  Hatano Y  Matsumoto H  Hirayama M  Konishi T 《Journal of agricultural and food chemistry》2005,53(1):145-150

For better understanding of the physiological function of anthocyanins, the absorption and metabolism of cyanidin 3-O-beta-D-glucopyranoside (Cy3G), which is one of the major anthocyanins in colored food materials, were precisely investigated. Combining two modalities newly developed, that is, highly sensitive semi-micro-HPLC and vein cannulation, Cy3G and its four major metabolites (M1-M4) were detected in the blood plasma of rats after oral administration of Cy3G (100 mg/kg of body mass). The plasma concentration of Cy3G reached its maximum at 15 min after the ingestion. Metabolite 2 (M2) and metabolite 3 (M3) showed their maximum plasma levels at 15 and 30 min, respectively, whereas metabolite 1 (M1) and metabolite 4 (M4) showed their maximum levels at 60 and 120 min, respectively. The maximum plasma concentrations of the four metabolites were in the following order: M3 (21 nM) > M4 (20 nM) > M1 (8.5 nM) > M2 (5 nM). When Cy3G was directly injected into the neck vein, only M2 and M3 were detected in the plasma, indicating that both M1 and M4 were produced during absorption from the gastrointestinal tract. Tandem MS analysis of the metabolites showed that M2 and M3 were monomethylated Cy3G, while M1 and M4 were glucuronides of Cy and methylated Cy, respectively. M3 was assigned as peonidin 3-O-beta-D-glucopyranoside (Pn3G) from the comparison of the retention time of authentic Pn3G.  相似文献   

Management strategy,uptake and translocation of nitrogen in tidal flooded rice ecosystem     

Md. Abdullah Al Mamun  M. Moynul Haque  M. Abu Saleque  Qazi Abdul Khaliq  A. J. M. Sirajul Karim  Md. Arifur Rahman Khan 《Journal of plant nutrition》2020,43(14):2186-2199

Abstract

Enhancing rice yield is a great challenge for rice growers in the tidal flooded ecosystem, where poor agronomic management is one of the major constrains. Improve management practice (IMP) was compared with traditional farmers’ practice (TFP) in evaluating rice productivity, nutrient uptake, translocation and farm income in tidal flooded ecosystem. Results revealed that, IMP significantly produced higher number of panicles m^?2, more grain panicle^?1 and better grain filling. The rice cultivars produced 2.0 to 2.5 t grain ha^?1 with TFP, while 3.0 to 4.0 t ha^?1 with IMP. In different rice cultivars, the grain yield in IMP increased 12 to 60% over TFP. Similarly, the grains in IMP treatment absorbed 21.41 to 57.03?kg N ha^?1 whereas only 15.85 to 46.94?kg N ha^?1 in TFP plot. However, higher nitrogen (N) transfer from shoot to grain in IMP also suggests that the amount of N in soil was too low to meet the plant demand in TFP. Although, the IMP involved additional cost, but it gave significantly higher gross return (438 to 954?US$ha^?1) and margin (397 to 913?US$ha^?1) which added farm income upto 225?US$ha^?1 over TFP. Hence, it could be concluded that IMP is a potential option for increasing grain yield and farm income during aman season in the tidal flooded ecosystem.  相似文献   

Estimating Electrical Conductivity for Soil Salinity Monitoring Using Various Soil-Water Ratios Depending on Soil Texture     

M. Shahadat Hossain  G. K. M. Mustafizur Rahman  A. R. M. Solaiman  M. Saiful Alam  M. Mizanur Rahman  M. A. Baset Mia 《Communications in Soil Science and Plant Analysis》2020,51(5):635-644

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Effect of hesperetin,a citrus flavonoid,on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats     

Cha  J.-Y.  Cho  Y.-S.  Kim  I.  Anno  T.  Rahman  S.M.  Yanagita  T. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2001,56(4):349-358

The effect of dietary hesperetin on the hepatic lipid content and the enzyme activities involved in triacylglycerol (TG) synthesis in rats fed diets with or without 1% orotic acid (OA) was studied. Hepatic TG content was raised by approximately 5-fold after administration of OA for 10 days. The OA-feeding significantly increased the activity of hepatic microsomal phosphatidate phosphohydrolase (PAP), which is the rate-limiting enzyme for TG synthesis. Hepatic glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme activities were also increased. An addition of 1% hesperetin to the OA-supplemented diet resulted in the decrease of the hepatic TG content by 44% and of microsomal PAP activity. Dietary hesperetin alone neither affected liver TG content nor PAP activity significantly. OA-feeding caused an increased liver cholesterol level, whereas simultaneous addition of hesperetin and OA reduced its content to the control level. A slight reduction of hepatic cholesterol by hesperetin was also observed in the OA-free dietary group. The present study demonstrated that dietary hesperetin can reduce the hepatic TG accumulation induced by OA, and this was associated with the reduced activity of TG synthetic enzyme, PAP.  相似文献   

Endophyte Effects on Nutrient Acquisition in Tall Fescue Grown in Andisols     

M. Hasinur Rahman  S. Saiga 《Journal of plant nutrition》2013,36(12):2141-2158

ABSTRACT

An experiment was conducted to evaluate the effect of endophyte Neotyphodium coenophialum (Morgan-Jones and Gams) Glenn, Bacon, and Hanlin on the mineral content of tall fescue (Lolium arundinaceum Schreb., SJ Darbyshire) ecotypes (Fukaura, Koiwai, and Showa) grown on two Andisols (Black Andisol and Red Andisol) with different fertilities. Black Andisol, with a naturally low content of phosphorus, was high in other nutrients, while Red Andisol, with a naturally high content of phosphorus, was low in other nutrients. Shoot dry weight was significantly higher in endophyte-infected (E+) than endophyte-free (E?) plants grown in Black Andisol. On the other hand, in Red Andisol, only Fukaura showed higher shoot dry matter production in E+ plants as compared to E- plants. In general, greater concentrations of phosphorus and manganese were found in endophyte-infected (E+) plants than non-infected (E?) plants. Accumulation of all nutrients in E+ plants was remarkably higher than in E? plants. Excluding the plant ecotype effect, the nutrient concentration and accumulation pattern for plants in the two soils was different in E+ and E? plants. Plants growing in Black Andisol had greater concentrations of phosphorus (P), copper (Cu), and zinc (Zn) in E+ plants than those of E? plants whereas for plants in Red Andisol the reverse was true. Significantly higher concentration of manganese (Mn) was found in E+ plants irrespective of soils. Regardless of endophyte and ecotypes, plants grown in Black Andisol showed significantly higher values for both nutrient concentration and accumulation than those in Red Andisol. Apart from the plant ecotype Showa, all other ecotypes had the greatest accumulation and concentration of phosphorus in E+ plants. In two types of soil and among the three plant ecotypes, endophyte infection had different effects on nutrients acquisition. Giving emphasis on nutrient accumulation, endophyte played an overall negative role on plants grown in Red Andisol. There may be a metabolic cost of harboring endophytes offset its benefit in Red Andisol. Since the same species of endophyte infected all three ecotypes, our results show that the host/endophyte interaction depends on plant ecotype as well as the ability of the soil to supply nutrients.  相似文献   

EFFECT OF BIODEGRADABLE CHELATING LIGAND ON IRON BIOAVAILABILITY AND RADISH GROWTH     

H. Hasegawa  M. Azizur Rahman  K. Saitoh  K. Ueda 《Journal of plant nutrition》2013,36(6):933-942

The effect of chelating ligands on iron (Fe) uptake and growth of radish (Raphanus sativus L.) was investigated. The ethylenediaminetetraacetic acid (EDTA) increased ⁵⁵Fe uptake in roots of radish though its subsequent translocation from roots to shoots and leaves did not increase. About 70%—80% of the total ⁵⁵Fe was distributed in the roots while about 5%—15% and 11%—17% were in shoots and leaves, respectively. The EDTA increased iron uptake into the roots of radish, but not in the above ground parts of the plant. The growth of radish (Raphanus sativus L.) decreased drastically in alkaline condition (pH > 9), even though the concentration of iron was sufficient in the growth medium. The growth of radish was enhanced successfully by the addition of hydroxyiminodisuccinic acid (HIDS) and EDTA. This might be because HIDS and EDTA solubilize iron from its precipitation with hydroxides at higher pH, and increase iron bioavailability. The influence of EDTA and HIDS on radish growth was comparable. Increase of radish growth by ethylenediaminedisuccinic acid (EDDS) and methylglicinediacetic acid (MGDA) was less than those by EDTA and HIDS. Considering the reproducibility of the radish growth (biomass production) at pH 10, HIDS is supposed to be more effective compared to EDTA.  相似文献