野生朱红硫磺菌驯化栽培研究   总被引：2，自引：2，他引：2  

曾先富  廖志勇  张军 《中国食用菌》2005,24(6):18-20

营养试验、菌丝体培养和出菇温度试验、培养基酸碱度试验、出菇条件试验结果表明：朱红硫磺菌菌丝体适宜生长的温度范围为15-32℃，最适温度范围为22～28℃；子实体发生的最适温度为16～25℃，气温低于16℃，子实体色泽为淡橙色，16℃以上，子实体呈鲜橙红色；培养料适宜营养C／N为20-26／1；朱红硫磺菌菌丝体适宜酸碱度范围为pH3．0～8．0，最适酸碱度为pH4．0—6．5；朱红硫磺菌的产量与光照条件无关，但光照强度影响子实体的发生时间和色泽；朱红硫磺菌栽培原料广泛，凡栽培木腐菌的培养料均可用于栽培朱红硫磺菌。  相似文献   

组织培养条件下苹果Ty1-copia类逆转座子的转录活性     

孙俊  房经贵  陶建敏  蔡斌华  章镇 《果树学报》2005,22(5):441-445

研究了苹果基因组中Tyl—copia类逆转座子的转录活性、多样性及其甲基化水平。在培养基MS＋2，4-D2mg／L＋BA0．2mg／L＋NAA0．5mg／L上诱导了1个月的愈伤组织中用RT—PCR方法检测到了270bp的目的条带，而对照、继代组培苗和不含2，4-D的愈伤中均没有目的条带的扩增，说明一定浓度的2，4-D可以诱导苹果中Tyl—copia类逆转座子的表达活性。将目的条带回收、克隆和测序后进行分析：所克隆的21条序列虽都为Tyl-copia类逆转座子逆转录酶保守序列，但存在很大的异质性，21个克隆的核甘酸序列变化范围为196—290bp；翻译成氨基酸后存在缺失、插入、移框和终止密码子突变，7个克隆在氨基酸保守序列‘SLYGLKQ’处有1—2氨基酸位点的突变，说明这些序列对应的逆转录酶多数可能已失去了其原有功能。将21条序列进行聚类分析，除ART20外，其它克隆可聚为3类，并分别与GenBank上某些物种的相应序列同源性很高，表明在不同物种间可能存在逆转座子的横向传递作用。甲基化试验表明苹果基因组内Tyl-copia类逆转座子甲基化水平高。这些都表明Tyl-copia类逆转座子对苹果基因型的多样性及基因组的进化具有重要意义。  相似文献   

柑橘裂皮病类病毒的一步RT-PCR法检测及其在甜橙体内的分布   总被引：3，自引：0，他引：3  

彭军  周常勇  唐科志  谭万忠 《果树学报》2005,22(6):744-747

柑橘裂皮病是由柑橘裂皮病类病毒(Citrusexocortisviroid,CEVd)引起的一种重要的柑橘病害。分别采用快速微量核酸提取法和SDS-KAc抽提法在表现症状的Etrog香橼中提取核酸,采用一步RT-PCR法对CEVd进行检测,结果显示SDS-KAc法可以消除带毒样品中非特异性条带。从嫁接接毒的铜水72-1锦橙植株的接穗部取嫩叶、老叶、皮,从砧木部茎杆上取老皮以及根皮分别提取总核酸进行RT-PCR检测,分析CEVd在甜橙体内的分布情况。初步检测结果表明在接穗的嫩叶、老叶、皮,砧木部茎杆的老皮和根皮上都可以检测到CEVd,以接穗部叶和皮检出稳定性高。  相似文献   

母猪卧向行为学的观察     

邓红雨  范佳英  包军 《家畜生态》2005,(5)

本文通过2个试验对母猪在分娩圈和分娩栏中的卧向行为进行了观察,试验一中, 选择10头大白(Yorkshire)经产母猪作为观察对象,试验二中分别选择10头大白(Yorkshire) 经产母猪和10头长白(Landrace)经产母猪作为观察对象。观察采用瞬时记录方法,每周观察 三次,隔日观察,每天上午、下午各观察一次,每次3 h,每次观察间隔5 min。观察中发现,母猪 卧向以向外为主。分娩栏(四周是栏杆)中的母猪以选择卧向外(向北)为主,其次是卧向内(向 南),卧向左和卧向右最少且差异不显著,上栏前和下栏后差异不显著。分娩圈(四周是墙壁)中 母猪以选择卧向外为主,卧向内最少,卧向左和卧向右差异不显著。长白母猪比大白母猪选择 卧向外的多,妊娠阶段比哺乳阶段选择卧向外的多。  相似文献   

菌根与植被恢复   总被引：5，自引：1，他引：5  

林晓民  李振岐  韩德俊  侯军 《干旱地区农业研究》2003,21(2):167-170

菌根不仅可促进植物的营养吸收、生长发育、抗病、抗逆,而且在保持良好的土壤结构、控制水土流失方面具有直接的作用。在我国西北许多地区,菌根菌与植物间的共生关系已被中断,要恢复该地区的植被,治本的方法应是重建植物与菌根菌的共生关系,形成健康的生态系统。文中还提出了菌根技术在植被恢复中的应用策略。  相似文献   

发菜形态特征及其与环境因子的关系   总被引：5，自引：0，他引：5  

唐进年  张盹明  聂文果  张锦春 《干旱区资源与环境》2003,17(1):123-128

通过野外调查和室内观察分析 ,天然发菜原植体的形态主要有柱状和带状两种 ;群体形态有单丝体、团块状、辫状、束状和网状。原植体的形态特征受环境湿度的影响较大 ,在降水量较大的地区 ,发菜原植体增粗 ,直径在 0 .0 5～ 0 .2 5 mm之间 ,带状发菜的比例较大 ,占 30～ 60 % ,吸水后藻体的颜色一般呈棕褐色 ;在降水量较小或极端干旱地区 ,发菜以细丝状为主 ,直径在 0 .0 2～ 0 .2 2 mm之间 ,带状发菜的比例不足 2 5 % ,吸水后藻体的颜色呈绿色或浅棕褐色。发菜群体形态的形成和发育与自身的生长特性有关外 ,主要与环境因子特别是降雨量、地形及自然外力 (地面径流和空气流动 )有关。  相似文献   

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro     

CAI Yun-feng  MIN Jun  FANG Tian-ling  CHU Zhong-hua  DENG Xiao-geng  HE Jing-song  CHEN Ji-sheng 《园艺学报》2003,19(8):1029-1033

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.  相似文献   

Effect of mutant β-catenin gene on the proliferation of hepatocytes     

SHANG Xian-zhang  MIN Jun  CHU Zhong-hua  CHEN Ji-sheng 《园艺学报》2003,19(12):1601-1605

AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth.  相似文献   

Powdery Mildew of Prairie Gentian: Characteristics,Molecular Phylogeny and Pathogenicity   总被引：3，自引：0，他引：3  

OKAMOTO  Jun  LIMKAISANG  Saranya  NOJIMA  Hidenobu  TAKAMATSU  Susumu 《Journal of General Plant Pathology》2002,68(3):200-207

In March 1999, we found prairie gentian (Eustoma grandiflorum) infected with powdery mildew in a greenhouse in Oita Prefecture, Japan. Morphological observation revealed that the causal fungus belongs to the mitosporic genus Oidium subgenus Pseudoidium [teleomorph: Erysiphe sensu Braun and Takamatsu (2000)]. Precise taxonomic position of the fungus, however, is uncertain due to lack of the perfect stage. We determined the nucleotide sequence of the rDNA ITS region of the fungus. Comparison of the sequence with those obtained from DNA databases of this fungal group revealed that the sequence is identical to those of powdery mildews from garden four-o'clock (Mirabilis jalapa) and broad bean (Vicia faba). Inoculation of an isolate from garden four-o'clock caused mildew on prairie gentian and broad bean, suggesting that the prairie gentian mildew originates from garden four-o'clock or broad bean. Molecular phylogenetic analysis indicated a close relationship of this fungus to Erysiphe baeumleri on Vicia spp. and E. trifolii on Trifolium pratense. From these results, we propose that prairie gentian mildew diverged from a Fabaceae-parasitic ancestor. Received 14 March 2002/ Accepted in revised form 28 May 2002  相似文献   

区域旅游资源开发定位研究——以内蒙古赤峰市为例   总被引：7，自引：1，他引：7  

吕君  刘丽梅 《干旱区资源与环境》2002,16(3):83-88

区域旅游资源开发是发展区域旅游的基础 ,开发的定位研究有助于为一地旅游业的发展指明方向。本文从区域角度和空间经济一体化思想出发 ,以赤峰市为例 ,通过对旅游资源的定量与定性研究 ,得到旅游资源的综合评价图式 ,结合区际分析归纳出赤峰市旅游资源开发利用的方向和层次。从而为区域旅游开发的定位研究进行了一般理论的探讨及例证分析  相似文献