排序方式： 共有28条查询结果，搜索用时 15 毫秒
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Jens B. Aune Adama Coulibaly Kamkam Woumou 《Archives of Agronomy and Soil Science》2019,65(3):400-410
This study focuses on the role of mechanised sowing and weeding in combination with seed priming and fertiliser microdosing in Mali. Mechanised sowing and weeding were based on using a combined donkey-drawn planter/weeder and a motorised planter/weeder. The research methods included studies of seed delivery in manual and mechanised sowing, field experiments on different levels of mechanization/intensification, labour studies on mechanisation and an economic assessment of the different levels of intensification.The average sorghum grain yield across three years increased by 352 kg ha?1 (43.7% increase) by combining mechanisation with seed priming and microdosing of 0.2 g NPK 15-15-15 fertiliser per pocket compared to a control with manual sowing but without seed priming and microdosing. The labour demand (sowing and weeding) for manual, donkey-drawn and motorised operations was 184, 67 and 47 hours ha?1, respectively.An economic analysis showed that the donkey-drawn planter/weeder is the appropriate mechanisation below six ha while above this land size it becomes increasingly interesting for the farmers to invest in a motorised planter. The use of mechanisation will result in earlier and uniform crop establishment, facilitate microdosing application, timelier weeding, higher yields, better economic return and reduced labour demand. 相似文献
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Plesker R Coulibaly C Hetzel U 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2002,49(8):428-429
In primates, little has been reported about malignant mesenchymal uterine tumours. A case of a spontaneous metastasising uterine sarcoma in a 17-year-old rhesus monkey is presented. Clinically, transient abdominal pain, spasms, nausea, anaemia, a firm uterus and bloody vaginal discharge were noted. In a diagnostic laparoscopy, both massive adhesions in the lesser pelvis and 10 ml of ascites fluid were detected. In necropsy, in addition to peritonitis with massive adhesions, a cauliflower-shaped, irregular, tough, greyish-white uterine tumour was seen. Two cherry-sized tumour metastases were noticed in the greater omentum. In histology, both in the uterus and the metastases, a sarcoma with a low amount of connective tissue and well-differentiated cell nuclei was identified. 相似文献
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P. Rainard Y. Lautrou P. Sarradin A. Coulibaly B. Poutrel 《Veterinary research communications》1991,15(3):163-176
The protein X of Streptococcus agalactiae is a surface antigen borne by a high proportion of strains isolated from bovine mastitis. We have tested the capacity of two strains of X-bearing Streptococcus agalactiae to induce mastitis in dairy cows. The reference X-strain (411.07) produced an intramammary infection with local clinical signs in the three inoculated quarters. Another X-bearing strain (443.31) of bovine origin produced infection in all 11 quarters inoculated with only 25 or 85 colony-forming units. In naive cows, strain 433.31 induced less exudation of plasma into the milk, shedding of bacteria, macroscopic alteration, and a lower somatic cell count (SCC) than did the reference strain. Only one quarter spontaneously eliminated the infection before antibiotic treatment 9 days after inoculation.The serum of all the cows contained naturally acquired or induced antibodies to the challenge strain (443.31) and possessed opsonic activity. Before inflammation occurred, the milk was almost devoid of antibody or opsonic activities. The early phase of infection was characterized by rapid multiplication of streptococci in the milk, followed by a sharp drop in bacterial counts concomitant with the onset of inflammation.Three cows immunized with protein X displayed higher SCC and bactericidal activity in milk from the inoculated quarter at the onset of inflammation than non-immunized cows. Two of the three immunized cows underwent an early and transient febrile episode and eliminated the infection. 相似文献
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Neafsey DE Lawniczak MK Park DJ Redmond SN Coulibaly MB Traoré SF Sagnon N Costantini C Johnson C Wiegand RC Collins FH Lander ES Wirth DF Kafatos FC Besansky NJ Christophides GK Muskavitch MA 《Science (New York, N.Y.)》2010,330(6003):514-517
Mosquitoes in the Anopheles gambiae complex show rapid ecological and behavioral diversification, traits that promote malaria transmission and complicate vector control efforts. A high-density, genome-wide mosquito SNP-genotyping array allowed mapping of genomic differentiation between populations and species that exhibit varying levels of reproductive isolation. Regions near centromeres or within polymorphic inversions exhibited the greatest genetic divergence, but divergence was also observed elsewhere in the genomes. Signals of natural selection within populations were overrepresented among genomic regions that are differentiated between populations, implying that differentiation is often driven by population-specific selective events. Complex genomic differentiation among speciating vector mosquito populations implies that tools for genome-wide monitoring of population structure will prove useful for the advancement of malaria eradication. 相似文献
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R G Hess C O Coulibaly I Greiser-Wilke V Moennig B Liess 《Veterinary microbiology》1988,16(4):315-321
A collection of 90 field isolates of hog cholera virus (HCV) was used to test the specificity of four hybridoma cell lines secreting monoclonal antibodies against pestiviruses. Reaction of virus isolates and monoclonal antibodies was controlled by an indirect immunofluorescence assay (IFA). Two monoclonal antibodies which had been generated against HC virus strain "Alfort 187" were reactive only with HCV field isolates and an HCV reference strain but not with bovine viral diarrhoea virus (BVDV) reference strains. Two other monoclonal antibodies (generated against BVDV, strain NADL) reacted only with BVDV reference strains but not with HCV field isolates, although with 3 of these strains focal reactions involving only a few cells were detected. The ability to discriminate between both viruses is a diagnostic need which may be fulfilled by these monoclonal antibodies. 相似文献
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In order to assess the effect of suckling regime on calf growth and milk yield and offtake of zebu cattle in Mali, 59 zebu cow–calf pairs were allocated to suckling regimes: RI, cows were milked completely and the milk was bottle-fed ad libitum to their calves; RII, calves were suckled ad libitum by their dams prior to milking; RIII, cows were milked partially then allowed to suckle their calves; RIV, ad libitum suckling of calves prior to milking during the first three months of lactation and partial milking prior to suckling thereafter. The highest lactation milk yield (1150.6 kg) and offtake (647.6 kg), but the poorest calf growth (261 g/d to weaning) were observed in RIII. RIV significantly improved calf growth, 310 g/d, while allowing a milk offtake of 294.4 kg. 相似文献
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Monoclonal antibodies (mab) specific for hog cholera virus (HCV), bovine viral diarrhoea virus (BVDV) or pestivirus were applied for the differential diagnosis of pestivirus infections in pigs. Field virus isolated from 8 confirmed classical swine fever outbreaks and one suspect case was propagated in PK(15) cell cultures and identified by direct immunofluorescence (IFA) and peroxidase linked antibody (PLA) assays. Peroxidase-linked HCV, BVDV and pestivirus specific mab were applied in direct PLA for differentiation. Nine isolates were classified as members of the genus pestivirus. Eight isolates showed a positive reaction with an HCV mab. One isolates reacted with BVDV specific mab only. For further characterization an indirect PLA was performed using a collection of different HCV and BVDV specific mabs. Some of the HCV isolates also showed a weak reaction with BVDV specific mab. 相似文献