排序方式： 共有78条查询结果，搜索用时 31 毫秒
61.
Sasaki K Oguma S Glass T Namiki Y Sugiyama H Ohmura N Blake DA 《Journal of agricultural and food chemistry》2008,56(17):7613-7616
In order to develop a rapid inexpensive test for cadmium in rice, we identified an antibody specific for cadmium-EDTA complexes; this antibody binds to cadmium-EDTA with a Kd of approximately 10(-8) M. Although the antibody's cross reactivity to magnesium was minimal (Kd approximately 10(-5) M), the high toxicity of cadmium coupled with the high natural occurrence of magnesium in rice resulted in a situation where magnesium interfered with cadmium determination and resulted in falsely elevated estimates of cadmium. Fortunately, the formation constant of EDTA for cadmium is approximately 5 x 10(7) times higher (at pH 7) than the formation constant of EDTA for magnesium, and we were able to eliminate the magnesium interference by judicious selection of the EDTA concentration used in the assay. The resulting equilibria are complex, but we show that a relatively simple two-step model in which cadmium and magnesium compete for EDTA followed by cadmium-EDTA and magnesium-EDTA competing for antibody provided a good fit to the measured data. These analyses enabled appropriate selection of the optimum EDTA concentration for an immunoassay with improved selectivity. 相似文献
62.
Naoya Ohtsuchi Tomohiko Kawamura Jun Hayakawa Hiroaki Kurogi Yoshiro Watanabe 《Fisheries Science》2018,84(2):211-225
Population dynamics of the kelp crab Pugettia quadridens, a potential predator of juvenile abalone, were investigated on the coast of Nagai, Sagami Bay, to examine its cryptic ontogenetic habitat shift. Monthly quantitative sampling over 2 years, which was carried out in 11 different subtidal habitats (< 0.1–8 m deep), revealed that P. quadridens changes ontogenetically according to its habitat: P. quadridens occurred in shallow lower subtidal (1–4 m) small red algal turfs (mainly Grateoloupia cornea) almost all year around, when the population was dominated by juveniles and stage I (immature) individuals; on the other hand, in the shallow upper subtidal (0.1–1 m) habitat (mainly Sargassum fusiforme beds near the low tidal mark), P. quadridens occurred only during winter to early spring, when the population was dominated by individuals of senior ontogenetic stages. These finding suggest that P. quadridens inhabit subtidal small red algal turfs during their early benthic phase, and then, the majority of larger individuals of stages II and III migrate to Sargassum beds. Our results suggest that the impact of P. quadridens predation on abalone stocks is limited in the present study site, primarily because the habitat of P. quadridens is segregated from that of juvenile abalone. 相似文献
63.
In yellow soybean, severe cold weather causes seed cracking on the dorsal side. Yellow soybeans carry the I or ii allele of the I locus and have a yellow (I) or pigmented (ii) hilum. We previously isolated an additional allele, designated as Ic, of the I locus, and reported that yellow soybeans with the IcIc genotype may be tolerant to cold-induced seed cracking. The Ic allele by itself, however, does not confer high tolerance. The association of a pubescence color gene (T) with suppression of low-temperature-induced seed coat deterioration has been previously reported. In the present study, we tested whether T is effective for the suppression of cold-induced seed cracking using two pairs of near-isogenic lines for the T locus in the iiii or IcIc background. In both backgrounds, the cracked seed rate of the near-isogenic line with the TT genotype was significantly lower than that with the tt genotype, which indicates that T has an inhibitory effect on cold-induced seed cracking. Furthermore, we also showed that gene pyramiding of Ic and T can improve tolerance to cold-induced seed cracking. Our findings should aid the development of highly SC-tolerant cultivars in soybean breeding programs. 相似文献
64.
Airanthi MK Sasaki N Iwasaki S Baba N Abe M Hosokawa M Miyashita K 《Journal of agricultural and food chemistry》2011,59(8):4156-4163
Brown seaweed lipids from Undaria pinnatifida (Wakame), Sargassum horneri (Akamoku), and Cystoseira hakodatensis (Uganomoku) contained several bioactive compounds, namely, fucoxanthin, polyphenols, and omega-3 polyunsaturated fatty acids (PUFA). Fucoxanthin and polyphenol contents of Akamoku and Uganomoku lipids were higher than those of Wakame lipids, while Wakame lipids showed higher total omega-3 PUFA content than Akamoku and Uganomoku lipids. The levels of docosahexaenoic acid (DHA) and arachidonic acid (AA) in liver lipids of KK-A(y) mouse significantly increased by Akamoku and Uganomoku lipid feeding as compared with the control, but not by Wakame lipid feeding. Fucoxanthin has been reported to accelerate the bioconversion of omega-3 PUFA and omega-6 PUFA to DHA and AA, respectively. The higher hepatic DHA and AA level of mice fed Akamoku and Uganomoku lipids would be attributed to the higher content of fucoxanthin of Akamoku and Uganomoku lipids. The lipid hydroperoxide levels of the liver of mice fed brown seaweed lipids were significantly lower than those of control mice, even though total PUFA content was higher in the liver of mice fed brown seaweed lipids. This would be, at least in part, due to the antioxidant activity of fucoxanthin metabolites in the liver. 相似文献
65.
Duong Van Hung Shengnan Tong Fumihiko Tanaka Eriko Yasunaga Daisuke Hamanaka Naoya Hiruma Toshitaka Uchino 《保鲜与加工》2011,(5):55
质量损失与冷害症状在新鲜农产品冷藏过程中经常发生,并会导致园艺产业巨大的经济损失。本试验分别研究了三种园艺产品,茄子(Solanum melongena)、日本芜青(Brassica rapa)和无花果(Ficus carica)在用两种类型加湿器制造的相对湿度为95%、温度分别为5.5℃和7℃的环境下贮藏10、6和8 d的贮藏品质。由纳米加湿器生成的水雾粒子平均直径小于100 nm,而由超声波加湿器生成的水雾粒子平均直径为216 nm。结果表明,纳米加湿器处理的日本芜菁、茄子和无花果样品的失重率分别为3.7%、5.3%和8.8%,超声波加湿器处理三种样品的失重率分别为7.3%、8.5%和14.7%。在纳米水雾贮藏室内放置的茄子果实的冷害指数要低于在超声波加湿环境中贮藏的果实。纳米水雾中的日本芜菁和无花果的气孔相比其初始状态下分别关闭了34.7μm2和51.5μm2,而在超声波水雾中则分别关闭了15.8μm2和25.5μm2。在纳米水雾中贮藏的日本芜菁的颜色要比在超声波水雾中贮藏的芫菁更绿。 相似文献
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67.
Takahashi N Kitamura K Matsuo N Mayford M Kano M Matsuki N Ikegaya Y 《Science (New York, N.Y.)》2012,335(6066):353-356
Synaptic inputs on dendrites are nonlinearly converted to action potential outputs, yet the spatiotemporal patterns of dendritic activation remain to be elucidated at single-synapse resolution. In rodents, we optically imaged synaptic activities from hundreds of dendritic spines in hippocampal and neocortical pyramidal neurons ex vivo and in vivo. Adjacent spines were frequently synchronized in spontaneously active networks, thereby forming dendritic foci that received locally convergent inputs from presynaptic cell assemblies. This precise subcellular geometry manifested itself during N-methyl-D-aspartate receptor-dependent circuit remodeling. Thus, clustered synaptic plasticity is innately programmed to compartmentalize correlated inputs along dendrites and may reify nonlinear synaptic integration. 相似文献
68.
Katsumi Takaba Teruyoshi Imada Shigehisa Katsumata Hiroshi Okumura Sachiko Iwamoto Yui Suzuki Minami Imaizumi Kensuke Myojo Chie Takada Naoya Kimoto Koji Saeki Itaru Yamaguchi 《Journal of toxicologic pathology》2012,25(4):265-271
This study histopathologically and immunohistochemically investigated a spontaneously
occurring single mass subcutaneously located in the left lower abdomen of a female
BALB/cAJcl−nu/+ mouse at 10 weeks of age. The mass was about 20 × 15 × 10 mm
in size after formalin fixation; nevertheless, it was not detected by clinical observations at
9 weeks of age. H&E staining revealed the tumor origin was epithelial and probably arose
from the mammary gland, and the tumor cells demonstrated a squamous, acinar or polyhedral/basal
pattern. A cell kinetics analysis revealed that many of the tumor cells of the squamous, acinar
or polyhedral/basal component were positive for PCNA and cyclin D1, although there were a few
of TUNEL-positive tumor cells in all of the components. An epithelial/mesenchymal analysis
demonstrated that most of the tumor cells of the squamous and acinar components contained
keratin and E-cadherin; however, most of the tumor cells of the polyhedral/basal component were
less or very weakly positive for these markers. The tumor cells of the squamous component were
negative for vimentin and SMA; however, many of the tumor cells of the polyhedral/basal
component exhibited vimentin. In addition, expression of SMA was confirmed in some tumor cells
of the acinar and basal components. Based on the microscopic and immunohistochemical
characterizations, the tumor was diagnosed to be adenosquamous carcinoma that originated from
the mammary gland with rapid growth, and the tumor cells demonstrated epithelial-mesenchymal
transition-like changes. 相似文献
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70.
Hiroshi BANNAI Yoshinori KAMBAYASHI Koji TSUJIMURA Tsuyoshi NAGASHIMA Naoya TAKEBE Masataka TOMINARI Manabu NEMOTO Minoru OHTA 《Journal of Equine Science》2021,32(3):99
The antibody response in horses inoculated with 2 doses of a live equine herpesvirus type 1 vaccine with different vaccination intervals (1 to 3 months) was evaluated with regard to the persistence of virus-neutralizing (VN) antibodies. The durations for which the geometric mean VN titers were maintained significantly higher than those before the first vaccination (P<0.05) were up to 5 months in horses that received the vaccination with a 1-month interval (n=17) and 7 months for those that received it with a 2-month (n=17) or 3-month interval (n=14 or 17). The vaccination program with the 2-month interval was the most effective in maintaining VN antibodies for a long duration with the smallest gap of antibody decline between the first and second vaccinations. 相似文献