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31.
Digestive proteolytic activities were studied in the larval midgut of Papilio demoleus by using azocasein, specific substrates and inhibitors regarding enzymatic allocation in the alimentary canal. The highest proteolytic activity was found in the midgut rather than foregut and hindgut that highest proteolytic activity was observed in soluble fraction. All specific proteases were almost equally distributed in both the anterior and posterior midgut except for trypsin- and chymotrypsin-like proteases, which showed the highest activity in the anterior midgut. Two peaks were observed in alkaline and acidic pHs when general proteolytic activities were assayed using azocasein in soluble and membrane-bound fractions. Specific substrates revealed the presence of trypsin-like, chymotrypsin-like, elastase, cathepsins B and L as well as two exopeptidases. These findings were confirmed using specific inhibitors including PMSF, TLCK, TPCK, SBTI, cystatin, DTT, E-64, EDTA and phenanthroline. Determination and characterization of the insect’s digestive proteases will enable development of pest control by designing protease inhibitors. This procedure will help us to designate an efficient pest control method with the lowest impact on the environment.  相似文献   
32.
The objective of the present study was to estimate genetic changes of body weight at different ages in Moghani sheep. Traits included were birth weight (BW, n = 4,208), 3-month weight (3MW, n = 4,175), 6-month weight (6MW, n = 3,138), 9-month weight (9MW, n = 2,244), and yearling weight (YW, n = 1,342). Data and pedigree information used in this study were collected at the Breeding Station of Moghani sheep during 1989–2005. The analysis was carried out for five traits, using the MTGSAM program. Breeding values of individual animals were obtained from a multivariate animal model analysis and genetic trends were obtained by regressing the means of predicted breeding values on year of birth for each trait. Direct genetic trends were positive and significant (P < 0.05) for BW, 3MW, 6MW, 9MW, and YW and were 1.63, 69.20, 79.38, 66.83, and 110.22 g/year, respectively. Also, maternal genetic trends for BW, 3MW, 6MW, 9MW, and YW were positive and significant (P < 0.05) and were 2.36, 49.18, 37.33, 17.73, and 9.67 g/year, respectively. The results showed that improvement of body weights of Moghani sheep seems feasible in selection programs.  相似文献   
33.
During a survey in 2011–2012, three ornamental plants of Araceae namely Aglaonema nitidum, Syngonium podophyllum and Dieffenbachia amoena showing foliar disease symptoms were collected from central region of Iran. Infected plants exhibited spots on their leaves which appeared as yellow and water-soaked with chlorotic haloes and necrotic center. To investigate the etiology of this disorder, symptomatic leaves were collected from affected plants and six bacterial strains (B2Y, J3Y, SY, E60Y, E68Y and E5MM) were isolated and identified as Pantoea ananatis or P. agglomerans based on morphological, physiological, biochemical and molecular characters. The pathogenicity tests of the isolates demonstrated that they were not host specific. Furthermore, 16S rRNA gene sequencing revealed that the strains were phylogenetically closely related to genus Pantoea. Multilocus sequence analysis (MLSA) of concatenated partial atpD, gyrB and rpoB gene sequences of the six isolates showed a high similarity of B2Y, J3Y, and SY strains to P. ananatis and also of E60Y, E5MM and E68Y strains to P. agglomerans. These results were confirmed by phylogenetic analysis. To the best of our knowledge, this is the first report of leaf spot and necrosis of A. nitidum, S. podophyllum and D. amoena caused by the genus Pantoea.  相似文献   
34.
Survival records from 1,763 Kermani lambs born between 1996 and 2004 from 294 ewes and 81 rams were used to determine genetic and non-genetic factors affecting lamb survival. Traits included were lamb survival across five periods from birth to 7, 14, 56, 70, and 90 days of age. Traits were analyzed under Weibull proportional hazard sire models. Several binary analyses were also conducted using animal models. Statistical models included the fixed class effects of sex of lamb, month and year of birth, a covariate effect of birth weight, and random genetic effects of both sire (in survival analyses) and animal (in binary analyses). The average survival to 90 days of age was 94.8%. Hazard rates ranged from 1.00 (birth to 90 days of age) to 1.73 (birth to 7 days of age) between the two sexes indicating that male lambs were at higher risk of mortality than females (P < 0.01). This study also revealed a curvilinear relationship between lamb survival and lamb birth weight, suggesting that viability and birth weight could be considered simultaneously in the selection programs to obtain optimal birth weight in Kermani lambs. Estimates of heritabilities from survival analyses were medium and ranged from 0.23 to 0.29. In addition, heritability estimates obtained from binary analyses were low and varied from 0.04 to 0.09. The results of this study suggest that progress in survival traits could be possible through managerial strategies and genetic selection.  相似文献   
35.
The effects of 1-methylcyclopropene (1-MCP) on ripening, superficial scald and concentrations of α-farnesene, conjugated trienols (CTols) and antioxidant enzyme activity of ‘KS6’ Asian pear (Pyrus serotina Rehd.) were studied. 1-MCP treated (2 μL L?1) or untreated control fruit were stored at 1 °C and 90–95% RH for up to 120 days. 1-MCP treated fruit were firmer than untreated fruit. Application of 1-MCP delayed skin color change. Scald appeared after shorter storage duration and was reduced, but not entirely controlled, with 1-MCP. Accumulation of α-farnesene and oxidation were slower in skin of 1-MCP treated fruit compared with controls. Catalase and peroxidase activities in untreated fruit either increased while activities decreased in 1-MCP treated fruit. Superoxide dismutase activity remained stable. The treatment of Asian pears with 1-MCP followed by cold storage maintained textural characteristics with less scald incidence.  相似文献   
36.
Recently a revival interest in the use of natural dyes in textile coloration has been growing. The major parts of natural dyes are anthraquinones. Depending on substituents, anthraquinone compounds are dyes, as represented by Alizarin Red S (ARS) which is a major constituent of the natural colorant madder. In this study, colorization of wool fiber by ARS as a natural dye was studied. The progress of the colorization process was followed spectrophotometrically at 500 nm as λ max. The effective factors on the process were investigated using one factor at a time (OFT) method. Then, the central composite design (CCD) method was applied to design experiments for the evaluation of the interactive effects of the four most important operating variables. The values of the optimized factors for OFT and CCD methods in wool dyeing with ARS were respectively as follow: temperature 70 °C and 55 °C, L/R: 40/1 and 40/2, pH 5.0 and 2.5 and time 60.0 min and 42.0 min. The predicted results by CCD had significantly higher exhaustion percentages and relatively better fastness properties than OFT and were found to be in a good agreement (R2=0.9908) with those obtained by performing experiments. Finally, the obtained results were shown a good wash fastness for dyed wool with ARS.  相似文献   
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Calving records from the Animal Breeding Center of Iran collected from January 1983 to December 2007 and comprising 1 163 594 Holstein calving events from 2552 herds were used to evaluate the potential effect of abortion occurrence on 305‐day milk yield, milk fat yield, fat percentage of milk and milk protein yield in Iranian Holsteins. Statistical analyses of production traits were performed using a linear mixed model procedure. Normal‐calved cows had greater 305‐day milk production, fat yield and protein yield of milk than abortive‐calved cows (P < 0.05). However, abortive‐calved cows had the greater milk fat percentage than normal calved cows (P < 0.05). The linear and quadratic effects of age of dam were significant on all of the studied traits (P < 0.05). The results of regression analysis showed that increase in the quadratic effect of age can cause an increase in the fat percentage of milk for all calvings including normal and abortive calvings. Because abortion is one of the most important conditions that limit a cow's ability to produce, maintaining the general health of cattle is important in minimizing the risk of abortion problems.  相似文献   
40.
Tropical Animal Health and Production - Iranian buffalo plays a critical role in supplying a portion of the income and the necessities of the rural population. The first step to design a breeding...  相似文献   
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