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11.
Uttam Kumar Arun K. Joshi Maya Kumari Rajneesh Paliwal Sundeep Kumar Marion S. Röder 《Euphytica》2010,174(3):437-445
Stay green or delayed senescence is considered to play a crucial role in grain development in wheat when assimilates are limited.
We identified three QTLs for stay green on the chromosomes 1AS, 3BS and 7DS using a recombinant inbred (RI) population developed
by making crosses between the stay green parent ‘Chirya 3’ and non-stay green ‘Sonalika’. The RI lines were evaluated in natural
field conditions for 2 years in replicated trial. The QTL on chromosome 1A was identified in both the years, while the QTLs
on 3BS and 7DS were identified only in 1st and 2nd year, respectively. The QTLs explained up to 38.7% of phenotypic variation
in a final simultaneous fit. The alleles for higher stay green values derived from the stay green parent ‘Chirya 3’. The QTLs
were named as QSg.bhu-1A, QSg.bhu-3B and QSg.bhu-7D. The QTL QSg.bhu-3B and QSg.bhu-7D were placed in the 3BS9-0.57-0.78 and 7DS5-0.36-0.61 deletion bins, respectively. 相似文献
12.
Chetan Paliwal Tonmoy Ghosh Khushbu Bhayani Rahulkumar Maurya Sandhya Mishra 《Marine drugs》2015,13(8):5384-5401
Phycobiliprotein-containing water and carotenoid-containing methanolic extracts of three different cyanobacteria, Pseudanabaena sp., Spirulina sp. and Lyngbya sp., were studied for their DPPH scavenging, iso-bolographic studies, and anti-nephrolithe activities. The best EC50 values for DPPH scavenging were in Lyngbya water (LW, 18.78 ± 1.57 mg·mg−1 DPPH) and Lyngbya methanol (LM, 59.56 ± 37.38 mg·mg−1 DPPH) extracts. Iso-bolographic analysis revealed most of the combinations of extracts were antagonistic to each other, although LM—Spirulina methanol (SM) 1:1 had the highest synergistic rate of 86.65%. In vitro digestion studies showed that DPPH scavenging activity was considerably decreased in all extracts except for Pseudanabaena methanol (PM) and LM after the simulated digestion. All of the extracts were effective in reducing the calcium oxalate crystal size by nearly 60%–65% compared to negative control, while PM and Spirulina water (SW) extracts could inhibit both nucleation and aggregation of calcium oxalate by nearly 60%–80%. 相似文献
13.
Biopsy material of the ileum and corresponding mesenteric lymph nodes from 10 naturally infected goats was studied. In ileum a loss of epithelial cells and infiltration of epitheloid cells, macrophages and a low number of lymphocytes, plasma cells and occasional eosinophils were seen. Ultrastructurally, the epithelial cells showed degenerative changes. Epitheloid cells were characterized by a large nucleus and a wide cytoplasm rich in free ribosomes.Macrophages had been fixed in the process of engulfing bacteria or contained bacteria in phagosomes and phagolysosomes. Large phagolysosomes were common. In macrophages with many or large phagolysosomes, few or no lysosomes were observed. Degenerative changes were seen in macrophages containing many bacteria. Degenerative changes of capillary endothelium were observed. The intercellular spaces were distended by oedema and contained cell debris.The mesenteric lymph nodes were infiltrated with epitheloid cells and macrophages. The ultrastructural picture of these cells was almost identical to that of the ileum.The differences between the changes found in naturally infected and experimentally infected animals are discussed. It is concluded that the mode of infection, the number of bacteria to which the animal is exposed and the intervals between events of exposure may play a role. 相似文献
14.
Studies were conducted on the effects of waters of different quality on the chemical characteristics of 74 surface and subsurface soils of the Nagaur district, Rajasthan. The electrical conductivity (EC) of the saturation extract of these sandy and loamy sand soils were found to be less than that of the irrigation water. Correlations were observed between EC and the constituent ions (Na+, Ca2+ and Mg2+ of the irrigation water and of the saturation extract of the soil samples. The pH values of the irrigation water and soil suspensions also showed a positive correlation. The carbonate and bicarbonate ion concentrations in both the irrigation water and the saturation extract of the soils correlated with their respective pH values. EC of both the irrigation water and the saturation extract from surface and subsurface soil layers correlated with total cations. Correlations between the chemical characteristics of the irrigation water and the saturation extract of the soils are of limited value for prediction purposes, due to other factors operating under field conditions. 相似文献
15.
Ten‐day‐old seedlings of six rice (Oryza sativa L.) cultivars were grown hydroponically for 30 days in a nutrient solution containing 222 μM aluminum (Al)/L. Leaf reflectance properties were determined at visible (400–700 nm) and near infrared (700–1100 nm) wavelengths under controlled environmental conditions. For the Al‐tolerant cultivars of rice, there was no significant difference in the visible and near infrared reflectances. By contrast, the Al‐susceptible cultivars showed a prominent increase in reflectance in both the visible (with minor shift in the peak) and in the near infrared region. Foliar chlorophyll content decreased significantly in the Al‐susceptible cultivars but not in the Al‐tolerant cultivars. Further, mineral uptake, uitilization efficiency, and pigment contents have been correlated with these reflectances. In addition, there was no correlation between foliar Al content and changes in the reflectance of the Al‐treated susceptible cultivars. 相似文献
16.
Landscape structure is often regarded as an important factor that governs the distribution and abundance of species.Therefore it is critical to understand the landscapes and their dynamics.Patterns of landscape elements strongly influence the ecological characteristics.This study was designed to document and map the current status of the tropical dry deciduous forest of the Tadoba-Andhari Tiger Reserve(TATR),Central India,(using IRS P6 LISS IV data)and to describe its landscape structure at three levels of organization viz.landscape,class,and patch.The study area was classified into 10 land cover classes that include 6vegetation classes.The landscape structure was analyzed using FRAGSTATS using 12 set of indices.The TATR landscapes have a total of2,307 patches with a mean patch size of 25.67 ha and patch density of 1.7patches per km2.Amongst all land cover classes,mixed bamboo forest is dominant—it occupied maximum area(77.99%)—while riparian forest is least represented(0.32%).Mixed forest has maximum number of patches among all vegetation classes.Results have shown that despite being dominant in the area,mixed bamboo forest has low patch density(0.25/100 ha).Dominance of mixed bamboo forest is attributed to large patch sizes and not to the number of patches.This study has focussed on the approach of integrating satellite forest classification and forest inventory data for studying forest landscape patterns. 相似文献
17.
A dose of 10(10) Mycobacterium avium subspecies paratuberculosis was administered orally on seven occasions to produce experimental paratuberculosis infection in 10 5-8-week-old goat kids. Bacteriological, immunological, and histopathological changes, their relationships, and the efficacy of the commonly used diagnostic methods were studied during the progressive disease up to 270 days postinfection (DPI). Significant lymphocyte proliferative responses in the peripheral blood of five goats were detected as early as 60 DPI. A lymphoproliferative test was also performed on lymphocytes purified from different compartments of the guts of five infected and five control goats. Significant proliferative responses were observed in lymphocytes of jejunal compartments of all five goats, of which four had also significant lymphocyte proliferation in the blood. The ileal lymphocytes from two goats, one each at 120 and 270 DPI, had significant proliferation. The histological lesions were mainly observed in the gut-associated lymphoid tissues of the ileocecal valve, the ileum, and the terminal jejunum. Acid-fast bacilli were demonstrated in the lesions of two goats at 60 and 210 DPI. Bacterial culture showed poor sensitivity, detecting positive results for only one goat in the fecal and tissue samples at 210 DPI, whereas polymerase chain reaction (PCR) detected one goat in fecal sample at 210 DPI and two goats in tissue samples at 60 and 210 DPIs, respectively. Enzyme-linked immunosorbent assay and agar gel immunodiffusion test were found to be 100% sensitive from 180 and 210 DPI onwards, respectively. 相似文献
18.
基于软X射线成像的储粮害虫米象生长阶段检测 总被引：1，自引：1，他引：0
为了准确检测单粒小麦内部是否感染米象（Sitophilus oryzae）,利用软X射线成像检测技术对感染不同生长阶段米象的小麦颗粒进行成像,试图通过图像分析来确定小麦内部米象的幼虫、蛹和成虫等不同生长阶段,并利用随机重复来评价结果可靠性。通过对被感染米象虫卵不同天数小麦的图像分析发现,图像灰度分布直方图随感染天数变化明显,低灰度值区域（灰度值为10～102）的灰度区域像素点随感染天数增加而减少,中灰度（灰度值为103～162）和高灰度区域（灰度值为163～232)则随感染天数增加而增多。使用包括图像灰度分布和纹理特征等47个特征值,利用线性判别分析（Linear Discriminant Analysis,LDA）与二次判别分析（Quadratic Discriminant Analysis,QDA）建立判别模型,并通过多次随机重复抽样（1 000次）对模型预测效果进行评估分析。结果表明：在95% 置信区间下,在感染与未感染小麦的分类判别中,LDA的判别准确率都在76%以上,除幼虫外生长阶段判别正确率达到95%以上;而QDA的平均判别准确率较低且判别误差也相对较高。因此,该研究使用基于随机重复的LDA判别小麦是否受到米象感染和区分不同生长阶段是准确可靠的。 相似文献
19.
Comparative efficacy of an IS900 tissue PCR, bacterial culture, johnin, agar-gel immunodiffusion (AGID) and absorbed-ELISA tests was investigated in 43 goats naturally infected with paratuberculosis. On histological examination, tissue sections from all animals showed typical granulomatous inflammatory changes. The lesions were classified as multibacillary (MB) (n=30), which had diffuse granulomatous lesions with abundant acid-fast bacilli (AFB), and paucibacillary (PB) (n=13), which had focal or multifocal granulomatous lesions with few AFB. The sensitivities of johnin test, tissue culture, faecal culture, tissue PCR, AGID and ELISA were 68% (17/25), 100% (30/30), 84.6% (22/26), 100% (30/30), 96.2% (25/26) and 100% (26/26) in MB goats, and 88.8 (8/9), 46.1% (8/13), 40% (4/10), 61.5% (8/13), 50% (5/10), and 70% (7/10) in PB goats, respectively. Except for the johnin test, which showed higher sensitivity in PB goats, all other tests displayed significantly higher sensitivities in MB goats. The results indicate the usefulness of tissue PCR, culture and serological tests in the diagnosis of clinically affected paratuberculous goats, especially with multibacillary pathology. 相似文献
20.
Ten‐day‐old seedlings of 22 rice (Oryza sativa.L) cultivars originated from various tropical countries were subjected to six levels of aluminum (Al) [0, 74, 148, 222, 296, and 370 μM] to test their tolerance to Al toxicity in nutrient solutions at pH 4.0±0.l. Seedlings were grown in the presence of Al under controlled environmental conditions in growth chambers. The nutrient solutions were replenished once a week. After 30 days, treatments were terminated and the differences in their growth patterns were compared. Standard growth parameters such as plant growth, dry matter production, relative growth reduction in roots (RGRS) and shoots (RGRS), root tolerance index (RTI) and shoot tolerance index (STI) have been used as markers of Al toxicity. Rice cultivars studied exhibited wide range of responses in their tolerance to Al. Though, the rice cultivars were subjected to six levels of Al, a good degree of separation in their responses was observed only at 222 μM Al. Therefore, this concentration was chosen to analyze and compare the performances of the cultivars. Further, only six cultivars showed significant changes in their expression in the presence of Al compared to control, and so data have been presented only for those cultivars for clarity. The cultivars BW 196, Bhura Rata, Basmati 370 and Co 37 recorded increases in growth, while Damodar and ADT 36 showed severe inhibitions in the presence of Al. Furthermore, in RTI and STI also Co 37 and Basmati 370 registered their tolerance to Al by showing increased growth in the presence of Al. Whereas, Damodar and ADT 36 recorded severe reductions. The RGRR and RGRS data also substantiates this finding. Based on the growth parameters, the six rice cultivars were ranked based on their tolerance to Al: Co 37 > Basmati 370 > BW 196 > Bhura Rata > Damodar > ADT 36. Co 37 and Basmati 370 are the two most tolerant cultivars which performed extremely well in the presence of Al, and Damodar and ADT 36 are the most susceptible cultivars. Therefore, the Al‐tolerant cultivars can be used for future breeding programes to develop Al‐tolerant, cultivars that subsequendy can be recommended for planting in acidic, infertile soils of the tropics. 相似文献