Influence of diet on growth,reproduction and lipid and fatty acid composition in the sea cucumber Cucumaria frondosa          下载免费PDF全文

Bruno L Gianasi  Christopher C Parrish  Jean‐François Hamel  Annie Mercier 《Aquaculture Research》2017,48(7):3413-3432

The suspension‐feeding sea cucumber Cucumaria frondosa is widely distributed in cold waters and is commercially exploited in the North Atlantic. While the species is considered to have potential for aquaculture, its feeding and reproductive biology differs markedly from that of currently cultivated sea cucumbers. Here, for the first time, the influence of food sources on the condition of C. frondosa was experimentally tested. Individuals were fed with either diatoms or fish eggs for 3 months. Specific growth rate (SGR), organ indices, fecundity, gonad maturity and profiles of lipids and fatty acids (FA) in tissues were compared among treatments and with sea cucumbers collected from the field. Individuals fed with fish eggs showed higher SGR and organ indices than all other treatments. The highest proportion of large oocytes was also found in gonad tubules of females from the fish egg treatment, although individuals fed with diatoms were the only ones in which spontaneous spawning occurred. Moreover, gonad and muscle tissues of sea cucumbers from the fish egg treatment presented the highest levels of lipids and essential FA. In contrast, non‐fed sea cucumbers showed negative SGR, relatively low female fecundity and low concentrations of lipids and FA in tissues. While the fish egg diet presented several obvious benefits, phytoplankton remains an important source of carotenoids, which are vital for vitellogenesis in echinoderms. This indicates that mixed diets rich in lipids, essential FA and carotenoids can be further investigated to optimize growth and reproductive output of this species in captivity.  相似文献   

Integration of continuous biofumigation with Muscodor albus with pre-cooling fumigation with ozone or sulfur dioxide to control postharvest gray mold of table grapes     

Franka Mlikota Gabler  Julien Mercier  J.I. Jiménez  J.L. Smilanick 《Postharvest Biology and Technology》2010,55(2):78-84

An integrated approach was evaluated that combined biological and chemical fumigation of table grapes to control postharvest gray mold caused by Botrytis cinerea. After fumigation of the grapes with ozone or sulfur dioxide during pre-cooling, the fruit were then exposed to continuous biofumigation by the volatile-producing fungus Muscodor albus during storage. Biofumigation was provided by in-package generators containing a live grain culture of the fungus. This grain formulation of M. albus survived the initial ozone or sulfur dioxide fumigation, but sulfur dioxide reduced its production of isobutyric acid, an indicator of the production of antifungal volatiles. Gray mold incidence was reduced among inoculated ‘Autumn Seedless’ grapes from 91.7 to 19.3% by 1 h fumigation with 5000 μL L^?1 ozone, and further reduced to 10.0% when ozone fumigation and M. albus biofumigation were combined. The natural incidence of gray mold among organically grown ‘Thompson Seedless’ grapes after 1 month of storage at 0.5 °C was 31.0%. Ozone fumigation and M. albus biofumigation reduced the incidence of gray mold to 9.7 and 4.4, respectively, while the combined treatment reduced gray mold incidence to 3.4%. The use of commercial sulfur dioxide pads reduced the incidence to 1.1%. The combination of ozone and M. albus controlled decay significantly, but was less effective than the standard sulfur dioxide treatments. Although less effective than sulfur dioxide treatment, ozone and M. albus controlled decay significantly, and could be alternatives to sulfur dioxide, particularly for growers complying with organic production requirements.  相似文献   

Impact of increasing shade levels on the dry-matter yield and botanical composition of multispecies forage stands     

Kelly M. Mercier  Christopher D. Teutsch  John H. Fike  John F. Munsell  Benjamin F. Tracy  Brian D. Strahm 《Grass and Forage Science》2020,75(3):291-302

Botanical diversity has been linked to increased biomass production of grasslands, but these relationships have not been explored as extensively in silvopasture systems where shade impacts on forage mass are variable due to the unique structure and environment of each system. The objective of this study was to evaluate the impact of multiple artificial shade levels on the DM yield and botanical composition of three cool-season forage mixtures near Blackstone, Virginia, USA. Mixtures were as follows: simple = tall fescue [Schedonorus arundinaceus (Schreb.) Dumort., nom. cons.] and white clover (Trifulium repens L.); intermediate = simple + orchardgrass (Dactylis glomerate L.) and red clover (Trifolium pretense L.); and complex = intermediate + Kentucky bluegrass (Poa pratensis L.), birdsfoot trefoil (Lotus corniculatus L.) and alfalfa (Medicago sativa L.). Slatted structures created conditions of 30%, 50% and 70% shade relative to a full sun control. Forages were harvested mechanically (no grazers present). Annual yield (DM kg/ha) did not differ among mixtures. As compared to full sun, annual yield was no different at 30% shade, but was reduced by 22 and 36% at 50 and 70% shade respectively. In contrast to other species in the mixtures, orchardgrass increased in proportion when grown beneath all shade levels and is recommended for silvopasture use. Orchardgrass is not particularly well-adapted to the transition zone between the northern temperate and southern subtropical United States; therefore, these results indicate that silvopastures may be an effective way to integrate marginally adapted, shade-tolerant cool-season forages into transition zone grazing systems.  相似文献   

Panleucopénie féline aiguë : à propos d’un cas traité avec succès par l’interféron-ω     

L.-A. Siméon  P. Mercier  P. Mazière  G. Bongrain 《Pratique Médicale et Chirurgicale de l'Animal de Compagnie》2009,44(4):125-131

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FANCM limits meiotic crossovers     

Crismani W  Girard C  Froger N  Pradillo M  Santos JL  Chelysheva L  Copenhaver GP  Horlow C  Mercier R 《Science (New York, N.Y.)》2012,336(6088):1588-1590

The number of meiotic crossovers (COs) is tightly regulated within a narrow range, despite a large excess of molecular precursors. The factors that limit COs remain largely unknown. Here, using a genetic screen in Arabidopsis thaliana, we identified the highly conserved FANCM helicase, which is required for genome stability in humans and yeasts, as a major factor limiting meiotic CO formation. The fancm mutant has a threefold-increased CO frequency as compared to the wild type. These extra COs arise not from the pathway that accounts for most of the COs in wild type, but from an alternate, normally minor pathway. Thus, FANCM is a key factor imposing an upper limit on the number of meiotic COs, and its manipulation holds much promise for plant breeding.  相似文献   

Influence of dietary fat and vitamin E on antioxidant status of muscles of turkey   总被引：2，自引：0，他引：2  

Renerre M  Poncet K  Mercier Y  Gatellier P  Métro B 《Journal of agricultural and food chemistry》1999,47(1):237-244

The aim of this study was to better understand the effects of more or less unsaturated fat source (tallow/soy oil/rapeseed oil) and/or vitamin E dietary supplementation (200 ppm) on the antioxidant status (at day 1 post-mortem) of turkey muscles [pectoralis major (Pm) and sartorius (S)]. More particularly, when turkeys were fed tallow, supplementation was sufficient to improve significantly the vitamin E status. Feeding rapeseed oil increased the antioxidant enzyme (AOE) activities (catalase, superoxide dismutase, glutathion reductase), glutathione concentration, and value from the benzoic acid test. Dietary soy oil increased glutathione peroxidase activity, compared to other dietary fat sources. With tallow, most of AOE activities were lower than with rapeseed or soy oil. Whatever the feeding mode, vitamin E supplementation did not affect the AOE activities, glutathione concentration, or values from the benzoic acid test. AOE activities were always higher in the oxidative S muscle than in the glycolytic Pm muscle. After feeding tallow, 9 days of storage increased TBA-RS and carbonyl contents, whereas the activity of many antioxidant enzymes and the total antioxidant activity (TEAC test and benzoic acid test) decreased.  相似文献   

Flow,mixing and residence time distribution of maize starch within a twin-screw extruder with a longitudinally-split barrel     

P. Colonna  J.-P. Melcion  B. Vergnes  C. Mercier 《Journal of Cereal Science》1983,1(2):115-125

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Prevalence of specific IgG-antibodies against Toxoplasma gondii in domestic turkeys determined by kinetic ELISA based on recombinant GRA7 and GRA8     

Koethe M  Pott S  Ludewig M  Bangoura B  Zöller B  Daugschies A  Tenter AM  Spekker K  Bittame A  Mercier C  Fehlhaber K  Straubinger RK 《Veterinary parasitology》2011,180(3-4):179-190

The protozoan parasite Toxoplasma (T.) gondii is one of the most common zoonotic infectious agents worldwide. Besides its sexual reproduction in cats, T. gondii can also infect a wide spectrum of other warm-blooded animals. These include animals used for human consumption such as pigs or chickens. Nevertheless, the role of turkeys for the epidemiology of T. gondii infections has not been studied thoroughly. We have established a kinetic ELISA (KELA) for the detection of T. gondii-specific IgG antibodies in turkey serum samples. The test is based on the recombinant dense granule antigens GRA7 and GRA8. These proteins were used as an antigen mixture at a concentration of 0.13 μg per well. The overall sensitivity of the assay was between 92.6% and 100% and the specificity ranged from 78.1% to 100%, depending on the method used to calculate these parameters. Using this KELA we examined 1913 turkey serum samples from 14 turkey farms from different areas of Germany. From these sera, 387 produced a signal in the KELA, corresponding to a true seroprevalence of up to 20.2%. The seropositivity rate in individual fattening cycles at individual farms ranged from 0.0% to 77.1%, whereas the rates were highly variable within the individual farms and individual fattening cycles. Consequently, conditions of animal husbandry could not be associated with particular seroprevalence rates. Although seropositivity cannot be linked directly to infectious tissue cysts in the muscle tissue of commercially produced turkey meat, we state that there is a potential risk of being infected by consuming turkey meat products that were not heat treated.  相似文献   

Effect of an inactivated paratuberculosis vaccine on the intradermal testing of goats for tuberculosis     

Chartier C  Mercier P  Pellet MP  Vialard J 《Veterinary journal (London, England : 1997)》2012,191(3):360-363

The effect of an inactivated paratuberculosis vaccine on the diagnosis of tuberculosis (TB) in goats was investigated in a herd with a history of clinical paratuberculosis but which was free of TB. Cohorts of animals in 2006, 2008 and 2009, were vaccinated once at 1 month of age, and 50% of the 2006 cohort served as unvaccinated controls. The goats were aged 8 months, 20 months and 3.5 years old at the time of the survey. All animals were assessed using a single intradermal injection of bovine tuberculin purified protein derivative (PPD) (SID test), or using both bovine and avian PPD (CID test). An interferon (IFN)-γ assay using both bovine and avian PPD was carried out on the 2006 cohort and was interpreted according to three different 'cut-off' points. No unvaccinated (control) animals tested positive to any of the assays, confirming that the herd was TB-free. The SID test had a low specificity in vaccinated animals at 8 and 20 months of age, whereas the CID test demonstrated 100% specificity in animals ≥20 months-old. The specificity of IFN-γ assay was less than maximal for vaccinated animals 3.5 years old as small numbers of false positives were detected, although this depended on the chosen cut-off point. The study findings demonstrate that the use of an inactivated paratuberculosis vaccine in goats <1 month-old in a TB-free herd does not result in false positives to a CID test for TB when performed in animals ≥20 months-old.  相似文献   

Prevention of sand fly attack by topical application of a permethrin/pyriproxyfen combination on dogs     

Mercier P  Jasmin P  Sanquer A 《Veterinary therapeutics : research in applied veterinary medicine》2003,4(3):309-316

Dogs are the primary domestic reservoir of Leishmania infantum, the parasite responsible for most cases of human visceral leishmaniasis. A strategy for the control of leishmaniasis would be to inhibit the sand fly bite. A study was designed to measure the prevention of the sand fly attack by spraying a combination of permethrin and pyriproxyfen on dogs artificially exposed to the vector of leishmaniasis. Eight dogs were individually challenged with 100 female sand flies for 1 hour on Days -7, 0, 7, 14, 21, and 28. Four dogs were randomly assigned to a control group and four dogs were treated with topically applied permethrin/pyriproxyfen on Day 0. After each exposure, sand flies were collected, counted, and scored as fed or unfed. Efficacy of the combination for prevention of feeding was based on the number of unfed sand flies (dead or alive). The combination of permethrin/pyriproxyfen demonstrated a significant (P <.05) repellent effect against Phlebotomus perniciosus bites as soon as it was sprayed on the dogs, and its repellent efficacy lasted at least for 28 days. The combination product provided significant (P <.05) knockdown activity against challenge with sand flies for 21 days in adult dogs and 14 days in puppies. These findings indicate that adult animals in endemic areas should be sprayed with the permethrin/pyriproxyfen product at 3- to 4-week intervals, and young dogs should be sprayed at approximately 2-week intervals, to prevent sand fly attack.  相似文献