Relative potencies of natural estrogens on vitellogenin and choriogenin levels in the Indian freshwater spotted snakehead, <Emphasis Type="Italic">Channa punctata</Emphasis>: in vivo and in vitro studies     

K. V. Rani  N. Sehgal  S. V. Goswami  Om Prakash 《Fish physiology and biochemistry》2010,36(3):587-595

The relative efficacies of three natural estrogens viz., estrone (E₁), estradiol-17β (E₂) and estriol (E₃) to induce synthesis of vitellogenin (Vg) and choriogenin (Chg) were assessed in primary hepatocyte cultures of the Indian freshwater spotted snakehead, Channa punctata. Hepatocytes were isolated from the spotted snakehead liver by a non-enzymatic protocol. Optimum culture conditions were standardized for ensuring their viability and functioning. Isolated hepatocytes were cultured for 48 h for monolayer formation and then exposed to various concentrations (0.001–10 μM) of the three estrogens. Competitive homologous ELISAs, developed and validated for spotted snakehead Vg and Chg were employed to determine the amounts of these two proteins secreted into the culture medium after 48 h of incubation. The results reveal that although all the three estrogens were effective in inducing the production of Vg and Chg in a dose-dependent manner, there were differences in their relative potencies. Of three estrogens, E₁ was the least potent and could induce synthesis of Vg and Chg only at a minimum concentration of 0.5 μM; whereas significant levels of both the proteins were quantified in culture medium by exposing the hepatocytes to E₂ or E₃ even at a concentration of 0.001 μM. All three estrogens were effective in inducing synthesis of Vg and Chg in vivo also. These results suggest the possibility of employing the above in vitro experimental design to monitor the presence of estrogens/estrogen-like chemicals in natural waters, which could interfere with the estrogen receptor system of fish. This study further points to the possibility of using Chg, in addition to Vg, as a parameter for screening various chemicals for their estrogenic activity.  相似文献   

Development and characterization of a cell line TTCF from endangered mahseer Tor tor (Ham.)     

Yadav K  Lakra WS  Sharma J  Goswami M  Singh A 《Fish physiology and biochemistry》2012,38(4):1035-1045

Tor tor is an important game and food fish of India with a distribution throughout Asia from the trans-Himalayan region to the Mekong River basin to Malaysia, Pakistan, Bangladesh and Indonesia. A new cell line named TTCF was developed from the caudal fin of T. tor for the first time. The cell line was optimally maintained at 28°C in Leibovitz-15 (L-15) medium supplemented with 20% fetal bovine serum (FBS). The propagation of TTCF cells showed a high plating efficiency of 63.00%. The cytogenetic analysis revealed a diploid count of 100 chromosomes at passage 15, 30, 45 and 60 passages. The viability of the TTCF cell line was found to be 72% after 6 months of cryopreservation in liquid nitrogen (-196°C). The origin of the cell lines was confirmed by the amplification of 578- and 655-bp sequences of 16S rRNA and cytochrome oxidase subunit I (COI) genes of mitochondrial DNA (mtDNA) respectively. TTCF cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids. Further, immunocytochemistry studies confirm its fibroblastic morphology of cells. Genotoxicity assessment of H?O? in TTCF cell line revealed the utility of TTCF cell line as in vitro model for aquatic toxicological studies.  相似文献   

Development of an ES-like cell culture system (RESC) from rohu, Labeo rohita (Ham.)     

M. Goswami  W. S. Lakra  Kamalendra Yadav  J. K. Jena 《Fish physiology and biochemistry》2012,38(6):1775-1783

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Interactive Effects of Nitrogen Source and Salinity on Growth Indices and Ion Content of Indian Mustard     

N. S. Nathawat  M. S. Kuhad  C. L. Goswami  A. L. Patel  Rakesh Kumar 《Journal of plant nutrition》2013,36(4):569-598

ABSTRACT

The interactions between salinity and different nitrogen (N) sources nitrate (NO₃ ^?), ammonium (NH₄ ⁺), and NO₃ ^? + NH₄ ⁺ were investigated on Indian mustard (Brassica juncea cv. RH30). Treatments were added to observe the combined effect of two salinity levels (8 and 12 ds m^{? 1}) and three nitrogen sources (NO₃ ^?, NH₄ ⁺, and NO₃ ^? + NH₄ ⁺) on different growth parameters and mineral composition in different plant parts, i.e., leaves, stem, and root. Salinity has been known to affect the uptake and assimilation of various essential nutrients required for normal growth and development. Different growth parameters, i.e., leaf area, dry weight of different plant parts, absolute growth rate (AGR), relative growth rate (RGR), and net assimilation rate (NAR) declined markedly by salinity at pre-flowering and flowering stages. All growth indices were less sensitive to salinity (12 d s m^{? 1}) with the nitrate form of nitrogen. It is pertinent mention that a high dose (120 kg ha^{? 1}) of nitrogen in ammonium form NH₄ ⁺, acted synergistically with salinity in inhibiting growth. Plants fed with combined nitrogen (NO₃ ^? + NH₄ ⁺) had an edge over individual forms in ameliorating the adverse effects of salinity on growth and yield. Under salt stress, different nutrient elements such as N, phosphorus (P), potassium (K⁺), and magnesium (Mg^{2 +}) were decreased in different plant parts (leaves, stem, and root). The maximum and minimum reduction was observed with ammoniacal and combined form of nitrogen, respectively, while the reverse was true of calcium (Ca^{2 +}), sodium (Na⁺), chloride (Cl^?), and sulfate (SO₄ ^2?) at harvest. Nitrogen application (120 Kg ha^{? 1}) in combined form had been found to maintain highest concentrations of N, P, Mg^{2 +}, and Ca^{2 +} along with reduced concentrations of Na⁺, Cl^?, and SO₄ ^{2 ?}. However, reverse was true with ammoniacal form of nitrogen.  相似文献   

Ultrastructural characteristics of the blood cells of chickens commonly reared under backyard poultry farming in Mizoram,India     

Probal Jyoti Doley  Kabita Sarma  Pranab Chandra Kalita  Ranjana Goswami  Arup Kalita  Rupan Sarkar  Chethan Gollahalli Eregowda  Parimal Roychoudhary  Om Prakash Choudhary 《Anatomia, histologia, embryologia》2023,52(2):223-233

The present study was undertaken to characterize the ultrastructural morphology of the blood cells of commonly reared chickens in the state of Mizoram, India under backyard poultry farming. For this study, 2 ml of whole blood was aseptically collected from the wings veins of 12 chickens of three different breeds namely the Zoar, Aseel and Rhode Island Red and processed for ultrastructural imaging under standard protocols. Under scanning electron microscopy (SEM) the matured erythrocytes of Zoar, Aseel and Rhode Island Red appeared elliptical in shape while the leukocytes and thrombocytes appeared round in shape with variable surface modifications. Under transmission electron microscopy (TEM) the granules of the heterophils of Zoar, Aseel and Rhode Island Red appeared predominantly fusiform in shape, the granules of the eosinophils appeared round in shape and that of the basophils appeared pleomorphic in shape. The cytoplasm of the monocytes, medium-to-large lymphocytes and thrombocytes of Zoar, Aseel and Rhode Island Red under TEM appeared to be vacuolated and granular while that of the small lymphocytes appeared to be granular but non-vacuolated. The study concluded that the ultrastructural characteristics of the blood cells of the three breeds of chicken studied were almost similar to the blood cells of other birds reported earlier.  相似文献   

公司责任及其在林产工业中的体现     

Rajat Panwar  Tomi Rinne  Eric Hansen  Heikki Juslin  管宁 《国际木业》2007,37(1):33-34

工商活动以及社会对其认识经历过很多显著的变化。中世纪流动的商人取代本地的工匠，满足社会对商品的需求是一大变革。社会对这些早期商人常取负面态度，认为他们进行剥削。工业革命使这种情况加剧。工商活动越来越专注于创造财富和谋求利润而置社会问题于不顾。随着时间的推移，社会管理调控的加强，工商活动的影响及其与社会及环境的相互关系逐渐被认识。自身的进化和社会的关注，使工商活动逐渐重新定向，承担起社会责任，造福于环境和社会。  相似文献   

Characterization of CO₂ Responsiveness in a Brassica oxycamp Interspecific Hybrid     

D. C. Uprety  Neeta Dwivedi  Rajat Mohan 《Journal of Agronomy and Crop Science》1998,180(1):7-13

A study of the characterization of CO₂ responsiveness in Brassica oxycamp and its parents Brassica oxyrrhina and Brassica campestris was done using open top chamber technology. The response of the X. B. oxycamp (hybrid) to elevated CO₂ was significantly positive in respect of photosynthesis and growth and similar to that of its parent B. campestris. X B. oxycamp and B. campestris with greater sink potential responded significantly, whereas B. oxyrrhina with a poor sink, did not respond to CO₂ enrichment. Photosynthetic changes at elevated CO₃ levels in the hybrid and parents are partially attributed to the CO₂ effects on stomatal conductance and chlorophyll fluorescence.  相似文献   

Cloning of 87 kDa outer membrane protein gene of Pasteurella multocida P52.     

P Chaudhuri  P P Goswami 《Research in veterinary science》2001,70(3):255-256

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The controlled evolution of a polymer single crystal     

Liu X  Zhang Y  Goswami DK  Okasinski JS  Salaita K  Sun P  Bedzyk MJ  Mirkin CA 《Science (New York, N.Y.)》2005,307(5716):1763-1766

We present a method for controlling the initiation and kinetics of polymer crystal growth using dip-pen nanolithography and an atomic force microscope tip coated with poly-dl-lysine hydrobromide. Triangular prisms of the polymer epitaxially grow on freshly cleaved mica substrates, and their in-plane and out-of-plane growth rates can be controlled by raster scanning the coated tip across the substrate. Atomic force microscope images were concomitantly recorded, providing a set of photographic images of the process as it spans the nanometer- to micrometer-length scales as a function of environmental conditions.  相似文献   

Hydrous Ferric Oxide (HFO)—a Scavenger for Fluoride from Contaminated Water     

Dey  Soumen  Goswami  Saswati  Ghosh  Uday Chand 《Water, air, and soil pollution》2004,158(1):311-323

Groundwaters contaminated with excess fluoride (above 1.5 mg/L) posed some risks to the public health in India. Methods available for fluoride contaminated water treatment are cogaulation–filtration using alum + lime + bleaching powder (Nalgonda technique) and adsorption using activated alumina. Use of aluminium compounds for water treatment purpose needs replacement on neurological health problem. Objective to this, the synthesis and fluoride adsorption behavior of hydrous ferric oxide (HFO) are reported here. It is seen that fluoride adsorption density varies as a function of pH, contact time, aging time, drying temperature and particle size of HFO. Highest adsorption density for fluoride is found to be at pH 4.0. Effects of competing anions in removing fluoride from solution were tested. Arsenite, arsenate, phosphate and sulfate show strong interfering effect at high anions to fluoride molar ratio in solution. Adsorption of fluoride on HFO follows the Freundlich isotherm and the Lagergren first-order kinetic model. It was also determined that HFO is a better adsorbent in removing fluoride from high fluoride groundwater than some other adsorbents. Regeneration of fluoride-rich HFO results showed that 1.0 M NaOH solution could be used up to a maximum of 75% regeneration.  相似文献