In vitro induction of vitellogenin by estradiol 17 β in isolated hepatocytes of catfish, Clarias gariepinus     

Rajendra Phartyal  L. Bibekananda Singh  S. V. Goswami  Neeta Sehgal 《Fish physiology and biochemistry》2005,31(2-3):241-245

Vitellogenin is a female-specific calcium-binding glycolipophosphoprotein synthesized in the hepatocytes of fishes. Its synthesis can be induced in fishes of either sex by estradiol or by xenoestrogens. To study the in vitro synthesis of vitellogenin, different culture conditions were set up using the hepatocytes of Clarias gariepinus. The present study reports on a non-enzymatic procedure for isolation and culture of hepatocytes from the liver of the catfish Clarias gariepinus, in order to study the effects of estradiol on vitellogenin synthesis in vitro. The procedure employs chelating properties of ethylenediamine tetracetic acid to achieve cell viability in excess of 95%. Equal numbers of isolated cells were incubated in different culture media viz. RPMI F1640, Medium-199, and Williams’ Medium E. At 36 h, cell attachment and monolayer formation is faster in M-199 and Williams’ Medium E than in RPMI. In order to study the effects of estradiol on vitellogenin synthesis, the isolated hepatocytes were seeded in Williams’ Medium E in 24-well cell culture plates. 17 β-estradiol (E₂) was introduced in the culture plates at different concentrations and for different time periods. The media were assayed for vitellogenin using competitive ELISA. Vitellogenin appeared in the medium after 48 h of incubation with 10⁻⁵ M estradiol whereas after 72 h of incubation 5×10⁻⁷ M E₂ could elicit the synthesis.  相似文献   

Wirkung von Harnstoff unter Zusatz von nitrifikationshemmenden Substanzen auf Stickstoffaufnahme und Ertrag von Reis in Abhängigkeit von der Placierung des Düngers und der Düngungszeit     

B. A. Lakhdive  Rajendra Prasad 《植物养料与土壤学杂志》1969,124(1):23-29

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Constituents of Musa x paradisiaca cultivar with the potential to induce the phase II enzyme,quinone reductase     

Jang DS  Park EJ  Hawthorne ME  Vigo JS  Graham JG  Cabieses F  Santarsiero BD  Mesecar AD  Fong HH  Mehta RG  Pezzuto JM  Kinghorn AD 《Journal of agricultural and food chemistry》2002,50(22):6330-6334

A new bicyclic diarylheptanoid, rel-(3S,4aR,10bR)-8-hydroxy-3-(4-hydroxyphenyl)-9-methoxy-4a,5,6,10b-tetrahydro-3H-naphtho[2,1-b]pyran (1), as well as four known compounds, 1,2-dihydro-1,2,3-trihydroxy-9-(4-methoxyphenyl)phenalene (2), hydroxyanigorufone (3), 2-(4-hydroxyphenyl)naphthalic anhydride (4), and 1,7-bis(4-hydroxyphenyl)hepta-4(E),6(E)-dien-3-one (5), were isolated from an ethyl acetate-soluble fraction of the methanol extract of the fruits of Musa x paradisiaca cultivar, using a bioassay based on the induction of quinone reductase (QR) in cultured Hepa1c1c7 mouse hepatoma cells to monitor chromatographic fractionation. The structure and relative stereochemistry of compound 1 were elucidated unambiguously by one- and two-dimensional NMR experiments ((1)H NMR, (13)C NMR, DEPT, COSY, HMQC, HMBC, and NOESY) and single-crystal X-ray diffraction analysis. Isolates 1-5 were evaluated for their potential cancer chemopreventive properties utilizing an in vitro assay to determine quinone reductase induction and a mouse mammary organ culture assay.  相似文献   

Effects of various organic amendments on organic carbon pools and water stable aggregates under a scented rice–potato–onion cropping system     

Rajeev Padbhushan  Rajiv Rakshit  Anupam Das  Rajendra Prasad Sharma 《Paddy and Water Environment》2016,14(4):481-489

Pools of organic carbon are quantified from the soil samples under scented rice crop from different soil layers (0–10, 10–20, and 20–30 cm) under 9 years’ long-term trials with five treatments in scented rice–potato–onion cropping system. These treatments were 100 % NPK (NPK), 50 % recommended NPK through mineral fertilizers + 50 % N as FYM (NPK + FYM), FYM + vermicompost (VC) + neem cake (NC) each equivalent to one-third of recommended N (FYM + VC + NC), 50 % N as FYM + biofertilizer for N + bone meal to substitute phosphorus requirement of crops + phosphate solubilizing bacteria (FYM + BFN + BM + PSB), FYM + vermicompost + neem cake each equivalent to 1/3rd of recommended N + PSB (FYM + VC + NC + PSB). SMBC (479 mg kg^?1), HWEOC (373 mg kg^?1), CWSCHO (235 mg kg^?1), HWSCHO (839 mg kg^?1), and ASCHO (180 mg kg^?1) were found to be the highest in the soil treated with FYM + VC + NC + PSB and the lowest with NPK. The quantity of hot water-extractable carbohydrate content is highest amongst cold water, dilute acid and hot water extractable carbohydrate that decreases with the soil depth irrespective of treatments, except CWEOC. Soil microbial biomass carbon (SMBC) shows significant correlation with CWEOC (r = 0.60**), HWEOC (r = 0.94**), CWSCHO (r = 0.75**), HWSCHO (r = 0.83**), and ASCHO (r = 0.83**) that primed for better aggregate stability irrespective of soil layers up to 30 cm depth. This indicates that labile carbon pools, most specifically water-soluble carbon, carbohydrate, microbial biomass, could be a suitable indicator for evaluation of soil quality, particularly in relation to soil aggregation.  相似文献   

New report of Citrus indica Yu. Tanaka,a wild progenitor species of citrus from Dailong Forest,Manipur, and recommendation for its conservation     

Devi  Elangbam Julia  Labala  Rajendra Kumar  Sanabam  Rakesh  Singh  Nandeibam Samarjit  Modak  Rahul  Devi  Huidrom Sunitibala 《Genetic Resources and Crop Evolution》2022,69(2):545-558

Genetic Resources and Crop Evolution - Citrus indica Yu. Tanaka is a wild and endangered progenitor species of citrus endemic to North East (NE) India. It is reported to be restricted to Nokrek...  相似文献   

Deep Tillage,Soil Moisture Regime,and Optimizing N Nutrition for Sustaining Soil Health and Sugarcane Yield in Subtropical India     

S. K. Shukla  R. L. Yadav  Rajendra Gupta  Akhilesh Kr Singh  S. K. Awasthi  Asha Gaur 《Communications in Soil Science and Plant Analysis》2018,49(4):444-462

A field experiment was conducted at ICAR-Indian Institute of Sugarcane Research, Lucknow, with three tillage practices (T₁: Control- two times ploughing with harrow and cultivator, each followed by planking before sugarcane planting; T₂: Deep tillage with disc plough (depth 25–30 cm) before planting followed by harrowing, cultivator, and planking; and T₃: Subsoiling at 45–50 cm and deep tillage with disc plough/moldboard plough (depth 25–30 cm) followed by harrowing, cultivator, and planking before planting, two soil moisture regimes (M₁: 0.5 irrigation water (IW)/cumulative pan evaporation (?CPE) ratio and M₂: 0.75 IW/CPE ratio) at 7.5 cm depth of IW, and four N levels (N₁- 0, N₂- 75, N₃- 150, and N₄-225 kg N ha^?1) in sugarcane plant crop. Deep tillage and subsoiling increased porosity and reduced bulk density in surface/subsurface soil. Further, these physical changes also improved soil biological and chemical properties responsible for higher crop growth and yield. Deep tillage and subsoiling reduced the compaction by 6.12% in 0–15 cm depth in sugarcane plant crop at maximum tillering stage. The highest N uptake (158.5 kg ha^?1) was analyzed with deep tillage and subsoiling compared to all other tillage practices. Maintaining suboptimal moisture regime with deep tillage and subsoiling showed the highest IW use efficiency (157.16 kg cane kg^?1 N applied). Mean soil microbial biomass carbon (SMBC) in ratoon crop was higher compared to plant crop. During initial tillering stage, ratoon crop showed higher SMBC with application of deep tillage and subsoiling (1209 mg CO₂-C g^?1 soil day^?1) at 0–15 cm depth and 1082.9 mg CO₂-C g^?1 soil day^?1 at 15–30 cm depth. Thus, it could be concluded that besides improving sugarcane yield, soil health could be sustained by adopting subsoiling (45–50 cm depth) and deep tillage (20–25 cm depth), with soil moisture regime of 0.75 IW/CPE and application of 150 kg N ha^?1 in sugarcane (plant crop).  相似文献   

Pattern of genetic inheritance of morphological and agronomic traits of sorghum associated with resistance to sorghum shoot fly, <Emphasis Type="Italic">Atherigona soccata</Emphasis>     

Riyazaddin Mohammed  Ashok Kumar Are  Rajendra S. Munghate  Anil Gaddameedi  Kavi Kishor Polavarapu Bilhan  Hari C. Sharma 《Euphytica》2018,214(2):32

Sorghum shoot fly, Atherigona soccata is an important pest of sorghum during the seedling stage, which influences both fodder and grain yield. To understand the nature of inheritance of shoot fly resistance in sorghum, we performed generation mean analysis using two crosses IS 18551 × Swarna and M 35-1 × ICSV 700 during the 2013–2014 cropping seasons. The F₁, F₂, BC₁ and BC₂ progenies, along with the parental lines were evaluated for agronomic and morphological traits associated with resistance/susceptibility to sorghum shoot fly, A. soccata. The cross IS 18551 × Swarna exhibited significant differences between the parents for shoot fly deadhearts (%) in the postrainy season. The progenies of this cross exhibited lower shoot fly damage, suggesting that at least one of the parents should have genes for resistance to develop shoot fly-resistant hybrids. Leaf glossiness, leafsheath pigmentation and plant vigor score during the seedling stage exhibited non-allelic gene interactions with dominant gene action, whereas 100 seed weight showed both additive and dominant gene interactions. Presence of awns showed recessive nature of the awned gene. Generation mean analysis suggested that both additive and dominance gene effects were important for most of the traits evaluated in this study, but dominance had a more pronounced effect.  相似文献   

Controlled-environment and field evaluation of heat stress induced genetic variability on translocation efficiency and grain filling traits in rice     

Ayyavu  Vinitha  Dhashnamurthi  Vijayalakshmi  Venugopal  Babu Rajendra Prasad 《Journal of Crop Science and Biotechnology》2022,25(5):535-545

Journal of Crop Science and Biotechnology - The grain filling of rice is gets weakened by heat stress due to reduced spike photosynthesis at high temperature stress. An alternate source of carbon...  相似文献