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121.
Ponder J Yoo BH Abraham AD Li Q Ashley AK Amerin CL Zhou Q Reid BG Reigan P Hromas R Nickoloff JA LaBarbera DV 《Marine drugs》2011,9(11):2397-2408
Type IIα DNA topoisomerase (TopoIIα) is among the most important clinical drug targets for the treatment of cancer. Recently, the DNA repair protein Metnase was shown to enhance TopoIIα activity and increase resistance to TopoIIα poisons. Using in vitro DNA decatenation assays we show that neoamphimedine potently inhibits TopoIIα-dependent DNA decatenation in the presence of Metnase. Cell proliferation assays demonstrate that neoamphimedine can inhibit Metnase-enhanced cell growth with an IC(50) of 0.5 μM. Additionally, we find that the apparent K(m) of TopoIIα for ATP increases linearly with higher concentrations of neoamphimedine, indicating ATP-competitive inhibition, which is substantiated by molecular modeling. These findings support the continued development of neoamphimedine as an anticancer agent, particularly in solid tumors that over-express Metnase. 相似文献
122.
Greenhouse and field experiments were conducted to evaluate the efficacy of various combinations of imazethapyr (0, 23, 46, and 70 g ai/ha) with glyphosate (0, 210, 420, 630, and 840 g ae/ha) on Setaria faberi, Amaranthus rudis, Abutilon theophrasti, and Ipomoea hederacea control. Additivity was the most frequently observed interaction and no synergistic interaction occurred throughout this study. The combination of imazethapyr with glyphosate at 210 g/ha caused an antagonistic response on Setaria faberi. Glyphosate at 420 g/ha with or without imazethapyr provided at least 95% control of Setaria faberi. The interaction between glyphosate and imazethapyr was additive on Amaranthus rudis control. Eight of the twenty-one herbicide combinations were antagonistic on Abutilon theophrasti control. Antagonistic interactions occurred when 46 or 70 g/ha of imazethapyr was added to 420 or 630 g/ha of glyphosate; while no antagonistic interactions were noted when glyphosate rate was 840 g/ha. The interactions on Ipomoea hederacea control were additive when the glyphosate rate was at least 420 g/ha. Glyphosate at 210 g/ha plus imazethapyr at 46 or 70 g/ha caused antagonistic interactions on Ipomoea hederacea control. Weed control tended to be more variable when the glyphosate rate was 210 g/ha and the imazethapyr rate was 46 or 70 g/ha. In general, the addition of imazethapyr to low rates of glyphosate improved control of Amaranthus rudis and Ipomoea hederacea and did not improve control of Setaria faberi and Abutilon theophrasti. 相似文献
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Association analysis reveals effects of wheat glutenin alleles and rye translocations on dough-mixing properties 总被引:2,自引:0,他引:2
Shusong Zheng Patrick F. Byrne Guihua Bai Xueyan Shan Scott D. Reid Scott D. Haley Bradford W. Seabourn 《Journal of Cereal Science》2009,50(2):283-290
The glutenin loci of wheat (Triticum aestivum L.) are important determinants of bread-making quality, although the effects of alleles at those loci are incompletely understood. We applied an association analysis method to assess the effects of glutenin alleles and 1RS wheat–rye (Secale cereale L.) translocations on dough-mixing properties in 96 wheat cultivars and advanced lines grown at three Colorado locations while accounting for population structure and relatedness of individuals in the population. The results indicated that (1) in the majority of cases, controlling relatedness of individuals reduced the significance of associations between glutenin loci and Mixograph traits; (2) the Glu-D1 and Glu-B3 loci and 1RS translocations had greater impacts on dough-mixing properties compared to other glutenin loci; (3) Glu-B1w, Glu-D1d, and Glu-B3b were consistently associated with greater (more favorable) Mixograph peak time (MPT) than other alleles at the respective loci, whereas Glu-B1e, Glu-D1a, and Glu-B3c were associated with reduced MPT; (4) the 1BL.1RS translocation was associated with a decrease in Mixograph properties. Our results indicate that taking multiple-level relatedness of individuals into account can improve the results of association analysis for wheat-quality traits. 相似文献
125.
Five methods that employed very different testing principles and procedures for assessing gluten quality were compared for 33 North American soft red and white wheats. The three methods analyzed flour (alveograph work, lactic acid solvent retention capacity, and mixograph peak time) and two methods employed ground wheat meal (Glutomatic gluten index and SDS sedimentation volume). Compared against the normalized mean of all five assessments, the ability of the assessment methods to evaluate gluten quality decreased in the order: alveograph work, lactic acid solvent retention capacity, mixograph peak time, Glutomatic gluten index, and SDS sedimentation volume. The methods utilizing flour were substantially superior predictive methods; however, the two meal‐based methods could be sufficient for early generation screening when flour is not available. 相似文献
126.
Groups of 8 steers weighing 300–400 kg (660–880 Ib) were rotationally grazed on a ryegrass-dominant pasture for 115 days. The systems compared were zero grazing (Z), field grazing conducted at the same stocking rate (FC), and field grazing conducted at a stocking rate varied with the intention of giving the same liveweight gain per uiimal as zero grazing (FV). Mean daily liveweight gains were: Z, 0.98; FC, 0.78; FV, 0.90 kg/animal (2.2, 1.7 and 20 Ib/animal), and liveweight gains/unit area were in the ratio 100:78:85. Organic-matter intake, measured on four occasions, was, on average, Z, 6.54; FC, 6.18; FV, 687 kg/head daily (14.4, 13.6 and 15.1Ib). From these results it appears that a comparison of zero grazing and field grazing made at the same stocking rate is likely to underestimate the potential of field grazing for beef production from grass. 相似文献
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