Characterization and growth conditions of Vibrio parahaemolyticus strains with different virulence degrees that cause acute hepatopancreatic necrosis disease in Litopenaeus vannamei     

Sonia A. Soto‐Rodriguez  Rodolfo Lozano‐Olvera  Daniel A. Palacios‐Gonzalez  Carmen Bolan‐Mejia  Karla G. Rendon‐Aguilar 《Journal of the World Aquaculture Society》2019,50(5):1002-1015

The phenotypic characteristics and growth kinetics at several temperatures, salinities, and pH values of three Vibrio parahaemolyticus (Vp) strains with different virulence and one nonpathogenic strain were evaluated. Independent of the virulence of the strain, a high metabolic diversity was found, which yielded different colored phenotypes on the CHROMagar? Vibrio. All strains were resistant to ampicillin and carbenicillin, and Vp AHPND+ organisms were the most sensitive to enrofloxacin. The exponential growth of Vp strains started at 1–2 hr of incubation, although no relationship was observed between the bacterial density and degree of virulence. Moreover, the growth of the most virulent strain was independent of the nutrients in the incubation media during the initial hour postinoculation. No strain grew at 4°C in 0% NaCl and pH 4, but only Vp AHPND+ grew at 44°C. For all strains, the lag phase was proportional to the NaCl concentration, and the growth was better at pH 8–9. However, the Vp AHPND? strain displayed a greater variability, was more sensitive to extreme conditions, and showed a lag phase of 9 hr independent of the pH.  相似文献   

The effects of sex and age on phytohaemagglutinin skin-testing of deer   总被引：1，自引：0，他引：1  

Fernandez-de-Mera IG  Vicente J  Hofle U  Rodriguez O  Gaspar-Lopez E  Gortazar C 《New Zealand veterinary journal》2008,56(2):71-73

AIM: To determine if there are sex- or age-related differences in the increase in skinfold thickness in response to the mitogen phytohaemagglutinin (PHA) in red deer. METHODS: One dose of 250 mug PHA was injected intradermally in the right side of the neck, and phosphate buffered saline (PBS) was injected at a second site as a control, in 110 (51 males and 59 females) captive Iberian red deer (Cervus elaphus hispanicus), ranging in age from 21 months to > or =5 years. Skinfold thicknesses were measured immediately before and 72 h following injection. RESULTS: There was a significant effect of gender on the average increase in skinfold thickness; males had greater increases (8.8 (SEM 0.57) mm) than females (4.23 (SEM 0.39) mm) after correcting for other confounding variables. No age-related differences were evident, but differences between sexes were more marked with increasing age. CONCLUSIONS AND CLINICAL RELEVANCE: Effects of gender, probably due to differences in energetic and reproductive constraints in red deer, should be taken into account when interpreting skinfold-test data, both in ecology and in the control of tuberculosis (Tb). Males tend to have a thicker skin than females, so skinfold increase relative to the thickness of the skin, rather than skinfold increase per se, should be used as a more appropriate measure of skinfold increase. This may also have clinical relevance in the interpretation of tuberculin skin testing.  相似文献   

Importance of house dust and storage mites in canine atopic dermatitis in the geographic region of Galicia, Spain     

Goicoa A  Espino L  Rodriguez I  Puigdemont A  Brazis P  Rejas J 《Acta veterinaria Hungarica》2008,56(2):163-171

Sensitisation to mites is frequent in atopic dogs. The main mite genus involved in canine atopic dermatitis is Dermatophagoides. The importance of storage mite allergens in dogs has been controversial. The aim of this study was to evaluate the sensitisation rates against storage mites (Lepidoglyphus destructor and Tyrophagus putrescentiae) and house dust mites (Dermatophagoides farinae and D. pteronyssinus) in atopic dogs from Galicia, a highly humid and temperate region of Spain, using a FcepsilonRIalpha-based immunoglobulin E (IgE) in vitro test. The study was performed on 95 dogs suffering from atopic dermatitis and presenting detectable specific serum IgE levels: 91.6% of the dogs tested positive for storage mites, whereas sensitisation to house dust mites was detected in 87.4%. These results indicate the importance of storage mites in this specific geographic area.  相似文献   

Preliminary findings in 30 dogs treated with micropulse transscleral cyclophotocoagulation for refractory glaucoma     

John S. Sapienza  Kay Kim  Elena Rodriguez  Nicola DiGirolamo 《Veterinary ophthalmology》2019,22(4):520-528

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Association of macrophage and lymphocyte infiltration with outcome in canine osteosarcoma     

Sita S. Withers  Katherine A. Skorupski  Daniel York  Jin W. Choi  Kevin D. Woolard  Renee Laufer‐Amorim  Ellen E. Sparger  Carlos O. Rodriguez  Stephen J. McSorley  Arta M. Monjazeb  William J. Murphy  Robert J. Canter  Robert B. Rebhun 《Veterinary and comparative oncology》2019,17(1):49-60

Immunotherapeutic strategies have shown promise for the treatment of canine osteosarcoma (cOSA). Very little is known about the immune microenvironment within cOSA, however, limiting our ability to identify potential immune targets and biomarkers of therapeutic response. We therefore prospectively assessed the disease‐free interval (DFI) and overall survival time (ST) of 30 dogs with cOSA treated with amputation and six doses of adjuvant carboplatin. We then quantified lymphocytic (CD3+, FOXP3+) and macrophage (CD204+) infiltrates within the primary tumours of this cohort using immunohistochemistry, and evaluated their association with outcome. Overall, the median DFI and ST were 392 and 455 days, respectively. The median number of CD3+ and FOXP3+ infiltrates were 45.8 cells/mm² (4.6‐607.6 cells/mm²) and 8.5 mm² (0‐163.1 cells/mm²), respectively. The median area of CD204+ macrophages was 4.7% (1.3%‐23.3%), and dogs with tumours containing greater than 4.7% CD204+ macrophages experienced a significantly longer DFI (P = 0.016). Interestingly, a significantly lower percentage of CD204+ macrophages was detected in cOSA arising from the proximal humerus compared to other appendicular bone locations (P = 0.016). Lymphocytic infiltrates did not appear to correlate with outcome in cOSA. Overall, our findings suggest that macrophages may play a role in inhibiting cOSA progression, as has been suggested in human osteosarcoma.  相似文献   

Repeated cross‐sectional study of Trypanosoma cruzi in shelter dogs in Texas,in the context of Dirofilaria immitis and tick‐borne pathogen prevalence     

Carolyn L. Hodo  Jessica Y. Rodriguez  Rachel Curtis‐Robles  Italo B. Zecca  Karen F. Snowden  Kevin J. Cummings  Sarah A. Hamer 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(1):158-166

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Pharmacokinetics of meloxicam after oral administration of a granule formulation to healthy horses     

Francisco Javier Mendoza  Juan Manuel Serrano‐Rodriguez  Alejandro Perez‐Ecija 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):961-967

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Nutritional evaluation and productivity of supplemented sheep grazing in semiarid rangeland of northeastern Brazil     

Araújo  A. R.  Rodriguez  N. M.  Rogério  Marcos Cláudio Pinheiro  Borges  I.  Saliba  E. O. S.  Santos  S. A.  Pompeu  R. C. F. F.  Fernandes  F. E. P.  Monteiro  J. P.  Muir  J. P. 《Tropical animal health and production》2019,51(4):957-966

Tropical Animal Health and Production - Sheep production systems in Brazilian caatinga rangelands require supplementation adapted to changes in floristic and chemical composition as dry seasons...  相似文献   

Prevalence and Characterization of Salmonella Isolated from Feral Pigs Throughout Texas          下载免费PDF全文

K. J. Cummings  L. D. Rodriguez‐Rivera  M. K. Grigar  S. C. Rankin  B. T. Mesenbrink  B. R. Leland  M. J. Bodenchuk 《Zoonoses and public health》2016,63(6):436-441

Feral pigs are one of the most abundant free‐roaming ungulates in the United States, yet their role in the ecology and transmission of foodborne pathogens is poorly understood. Our objectives were to estimate the prevalence of Salmonella shedding among feral pigs throughout Texas, to identify risk factors for infection, and to characterize the isolates. Faecal samples were collected from feral pigs in Texas from June 2013 through May 2015. Standard bacteriologic culture methods were used to isolate Salmonella from samples, and isolates were characterized via serotyping and anti‐microbial susceptibility testing. The prevalence of faecal Salmonella shedding among sampled pigs was 43.9% (194/442), with positive pigs originating from 50 counties. Pigs sampled during fall and summer were significantly more likely to be shedding Salmonella than pigs sampled during winter. High serovar diversity was evident among the isolates, and many of the detected serovars are leading causes of human salmonellosis. The most common serovars were Montevideo (10.0%), Newport (9.1%), and Give (8.2%). Resistance to anti‐microbial agents was rare. The burgeoning feral pig population in the United States may represent an emerging threat to food safety.  相似文献   

Does Salinity Inhibit Alfalfa Leaf Growth by Reducing Tissue Concentration of Essential Mineral Nutrients?   总被引：3，自引：1，他引：2  

H. A. Esechie  V. Rodriguez 《Journal of Agronomy and Crop Science》1999,182(4):273-278

The increased salinity of arid and semi-arid lands has become a problem of great concern in agriculture. The possibility that the inhibition of leaf growth in alfalfa (Medicago sativa) growing under saline conditions may be associated with a reduction in ion concentration in the leaf was investigated. Seeds of alfalfa cv. Buraimi were sown in PVC cylinders filled with loamy sand and arranged in large pots embedded in the soil with the rim just above the soil surface. This arrangement simulated plants grown under field conditions. Seedlings were irrigated with saline (12.2 dS m−1) and nutrient solutions (full strength or 20 times dilution) twice weekly. After 6 weeks the plants were harvested and separated into roots, stem and leaves. The leaves were further separated into lamina and petioles and analysed for the major and minor nutrient elements. Salinization significantly reduced the fresh weights of the roots, stem and leaves as well as the concentration of N, K, Ca and Mg in the leaf tissue. With the exception of Cu concentration, which was enhanced by salinity, all the micronutrients (B, Zn, Mn and Fe) had reduced concentrations in the leaves of salinized alfalfa. The application of an external nutrient solution, especially the full-strength solution, produced an increase in leaf growth and leaf nutrient element concentration. On the basis of these results it was concluded that reduced leaf nutrient concentration may be one of the primary causes of the inhibition of leaf growth that was characteristic of alfalfa growing under salinity stress.  相似文献