Thermal tolerance of a thermally selected strain of rainbow trout <Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis> and the pedigrees of its F1 and F2 generations indicated by their critical thermal maxima     

Toshinao Ineno  Koichi Tamaki  Kazuya Yamada  Ryusuke Kodama  Shuji Tsuchida  Engkong Tan  Shigeharu Kinoshita  Koji Muto  Takashi Yada  Shoji Kitamura  Shuichi Asakawa  Shugo Watabe 《Fisheries Science》2018,84(4):671-679

A thermally selected strain of rainbow trout has been established by selective breeding since 1966 in Miyazaki, Japan. In the present study, we compared the critical thermal maxima (CTMs), the temperatures at which organisms reach a predefined sublethal endpoint and lose their equilibrium, between a thermally selected and two normal (Donaldson) strains of rainbow trout. The CTM of one normal strain from Nikko (Nikko strain) acclimated to 20 °C (29.7 °C) was significantly lower than those of the thermally selected strain (30.0 °C) and the other Donaldson strain from Aomori (29.9 °C) (P?<?0.05). The F1 generations, F1T and F1N, were produced by crossing thermally selected strain females with Nikko strain males and Nikko strain females with thermally selected strain males, respectively. No significant difference was observed in the CTM between F1T [30.1?±?0.15 °C (n?=?30)] and F1N [30.1?±?0.16 °C (n?=?30)] (P?>?0.05) for fish acclimated to 20 °C, suggesting that the F1 offspring inherited the thermal tolerance trait from one thermally selected strain parent irrespective of whether it was the male or female. F2 offspring of F1T or F1N also showed the thermal tolerance trait. The coefficients of variation for CTM were also compared among all the datasets obtained in the present study and their values for F1 hybrids were lower than those of the parental generation of the Nikko strain (P?<?0.05). In contrast, the coefficients of variation of F2s were the same as those of their parental generation. Furthermore, the thermally selected strain and Nikko strain as a reference family provide a F2 generation for segregating phenotypes, which is required for in-depth genetic analysis of the thermally selected rainbow trout strain.  相似文献   

Work-related increases in titer of Campylobacter jejuni antibody among workers at a chicken processing plant in Miyazaki prefecture,Japan, independent of individual ingestion of edible raw chicken meat     

Elisabet TANGKONDA  Meiko KUBO  Satoshi SEKIGUCHI  Taisuke SHINKI  Satomi SASAKI  Kentaro YAMADA  Takako TANIGUCHI  Torrung VETCHAPITAK  Naoaki MISAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1306

Workers in poultry abattoirs may be frequently exposed to Campylobacter jejuni, which is a leading cause of bacterial food poisoning in Japan. The present study was conducted to measure the titers of IgG and IgA antibodies against C. jejuni among 104 female workers in a chicken processing plant in Miyazaki prefecture, Japan. Information regarding habitual ingestion of raw chicken meat and potential occupational risk factors was collected using a questionnaire. Acid extracts of four C. jejuni strains representing the genotypes most dominant in Miyazaki were used as antigens. The levels of both immunoglobulins measured by ELISA were not correlated with ingestion of edible raw chicken meat, the amount consumed in one sitting, or its frequency. Although age was correlated with antibody levels, the length of employment was not. Furthermore, the IgG and IgA levels in workers at the evisceration step were significantly higher than those at other locations in the plant. To identify the bacterial proteins recognized by the workers’ IgG and IgA antibodies, Western blotting followed by LC/MS was conducted. Flagellin was identified as the common protein recognized in the sera of workers for whom ELISA demonstrated both the highest and lowest antibody levels. We concluded that the titers of IgG and IgA against C. jejuni in workers at the processing plant had been increased by occupational exposure to Campylobacter, regardless of raw chicken meat ingestion.  相似文献   

Effect of different ions on larval metamorphosis of the mussel Mytilus galloprovincialis          下载免费PDF全文

Jin‐Long Yang  Shu‐Heng Li  Wei‐Yang Bao  Hideki Yamada  Hitoshi Kitamura 《Aquaculture Research》2015,46(1):155-162

Metamorphic responses of pediveliger larvae of Mytilus galloprovincialis to different ions were investigated through a series of bioassays. Effects of tetraethylammonium chloride (TEA) on inductive effects of these above ions were also investigated. Excess ions including Li⁺, Cs⁺, Rb⁺ and Ca²⁺ induced larval metamorphosis at 10^?3 M to 5 × 10^?2 M in 24‐h exposure assays. In continuous exposure assays, only excess Ca²⁺ showed inductive activity and induced 25% metamorphosis at 5 × 10^?2 M. Larval responses to Li⁺ and Rb⁺were inhibited by TEA, while induction of metamorphosis by Cs⁺and Ca²⁺ was independent of the presence of TEA. Thus, these ions used can be useful inducers of larval metamorphosis for application in the aquaculture industry. The finding provides new insights on the biochemical mechanism controlling larval metamorphosis in this species.  相似文献   

Modeling of Salt Sorption in Volcanic Ash Soil     

Kei Nakagawa  Shin-Ichiro Wada  Ryoma Kitamura  Ronny Berndtsson 《Communications in Soil Science and Plant Analysis》2017,48(21):2594-2600

Groundwater pollution by nitrate from agricultural fields is a worldwide problem. To improve the understanding of nitrate transport processes through volcanic ash soils overlying groundwater aquifers, salt sorption experiments were performed. Salt sorption is the process that involves the simultaneous use of cation and anion adsorption mechanisms without changing the pH. Prepared six different concentrations of potassium chloride (KCl) solutions were mixed with soil samples. Anion and cation contents of the liquid and solid phases were determined. Experiments were performed in triplicate of six kinds of KCl solutions for three types of soils. Thus, total 54 of samples were analyzed. Relationships between ionic strength and sorption capacity increments of cations and anions were determined. The results show that if ionic strength of the infiltrating solution to the volcanic ash soil is known, the ECEC (effective cation exchange capacity) and EAEC (effective anion exchange capacity) can be reliably estimated. The resulting prediction equations can be included to improve reactive transport simulation models. Consequently, it will be possible to better understand solute transport with changing sorption capacity for the solid soil phase.  相似文献   

Transepithelial Transport of Theasinensins through Caco-2 Cell Monolayers and Their Absorption in Sprague-Dawley Rats after Oral Administration     

J Qiu  Y Kitamura  Y Miyata  S Tamaru  K Tanaka  T Tanaka  T Matsui 《Journal of agricultural and food chemistry》2012,60(32):8036-8043

The aim of this study is to illustrate the in vivo and in vitro absorption of theasinensins B and A that are (-)-epigallocatechin-3-O-gallate (EGCG)-(-)-epigallocatechin (EGC) dimer and EGCG dimer, respectively, and their transport pathway across the intestinal membrane. Our animal study by a single oral administration to rats demonstrated the intact absorption of theasinensins into the blood system, which was estimated to be a >10-fold lower absorption amount than EGCG. The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their permeability coefficients were also >10-fold lower than those of EGCG and EGC. Transport experiments using cytochalasin D or quercetin as a tight junction (TJ) modulator and a non-saturable permeation revealed that theasinensins were transported across Caco-2 cells in a TJ paracellular diffusion route. In conclusion, the dimers of condensed catechins, theasinensins B and A, can be absorbed intact into rat blood and transported across Caco-2 cell monolayers probably through a TJ paracellular pathway.  相似文献   

Evaluation of transplantation of autologous bone marrow stromal cells into the cerebrospinal fluid for treatment of chronic spinal cord injury in dogs     

Nishida H  Nakayama M  Tanaka H  Kitamura M  Hatoya S  Sugiura K  Suzuki Y  Ide C  Inaba T 《American journal of veterinary research》2011,72(8):1118-1123

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Increased concentration of high-mobility group box 1 protein in milk is related to the severity of bovine mastitis     

Furukawa Y  Hayashi T  Mizuta M  Ebara S  Kiku Y  Ozawa T  Matsubara T  Ito I  Kitamura D  Mizuta R 《Veterinary research communications》2011,35(1):47-54

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Histopathologic changes associated with Meibomian Gland dropout in a dog     

Yasunari Kitamura  Reiko Arita  Yukihiro Miwa  Hiroko Iwashita  Akihiko Saito 《Veterinary ophthalmology》2020,23(3):575-578

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Spontaneous hyaline glomerulopathy in a young Slc:ICR mouse     

Hironobu Nishina  Akane Kashimura  Tetsuya Sakairi  Satomi Nishikawa  Mao Mizukawa  Hiroko Sato 《Journal of toxicologic pathology》2020,33(4):303

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Continuous exposure to α-glycosyl isoquercitrin from developmental stages to adulthood is necessary for facilitating fear extinction learning in rats     

Yasunori Masubuchi  Junta Nakahara  Satomi Kikuchi  Hiromu Okano  Yasunori Takahashi  Kazumi Takashima  Mihoko Koyanagi  Robert R. Maronpot  Toshinori Yoshida  Shim-mo Hayashi  Makoto Shibutani 《Journal of toxicologic pathology》2020,33(4):247

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