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21.
Takeshi Takasaki Kazuma Okada Carlos Castillo Yuki Moriya Toshihiro Saito Yutaka Sawamura Naoko Norioka Shigemi Norioka Tetsu Nakanishi 《Euphytica》2004,135(2):157-167
A new S
9-allele was discovered in 6 Japanese pear cultivars, ‘Shinkou’, ‘Shinsei’, ‘Niitaka’, ‘Amanogawa’, ‘Nangetsu’ and ‘Nansui’.
cDNA encoding S
9-RNase, a stylar product of S
9-allele, was cloned from pistils of ‘Shinkou’ and ‘Shinsei’ by 3' and 5' RACE. The S
9-RNase gene had an open reading frame of 684 nucleotides encoding 228 amino acid residues. S
9-RNase had a hypervariable (HV) region different from S
1- to S
8-RNase and shared higher similarity (95.2%) with apple S
3-RNase than with 8 Japanese pear S-RNases (from 61.0% to 70.7%). Genomic PCR with primers ‘FTQQYQ’ and ‘anti-(I/T) IWPNV’ provided S
1- to S
9-amplicon (product), but could not discriminate the S
2 from the S
9 of ca. 1.3 kb. The S
2 and S
9 were distinguished by digestion with AflII and BstBI, respectively. The digestion with nine S-allele-specific restriction endonucleases, SfcI, AflII, PpuMI, NdeI,AlwNI, HincII, AccII, NruI and BstBI, distinguished S
1 to S
9, establishing that this PCR-RFLP system is useful for S-genotype assignments in Japanese pear harboring S
1- to S
9-allele. ‘Shinkou’, ‘Shinsei’, ‘Nangetsu’ and ‘Nansui’ assigned as S
4
S
9 were determined to be cross incompatible.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
22.
Summary One-hundred and five cultivars representing s species of Capsicum were screened at constant 13, 16, 18°C and greenhouse temperatures. Daily observation on emergence were made for 60 days following seeding in flats.Using a calculated emergence index as the criterion for emergence at low temperatures, the various cultivars were classified by species. Within the C. annum group significant differences were found between cultivars for the lowest and highest index suggesting heritability of this trait. The fowest indexes for all temperatures was a cultivar from the C. baccatum v. pendulum group.Michigan Agricultural Experiment Station Journal No. 8132.Present address: 2306 1/2 Murray Avenue, Pittsburgh, PA 15217, USA. 相似文献
23.
We prepared 31 monoclonal antibodies (MAbs) against either FIPV strain 79-1146 or FECV strain 79-1683, and tested them for reactivity with various coronaviruses by indirect fluorescent antibody assay (IFA). Sixteen MAbs which reacted with all of the 11 strains of feline coronaviruses, also reacted with canine coronavirus (CCV) and transmissible gastroenteritis virus (TGEV). In many of them, the polypeptide specificity was the recognition of transmembrane (E1) protein of the virus. We succeeded in obtaining MAbs which did not react with eight strains of FIPV Type I viruses (showing cell-associated growth) but reacted with FIPV Type II (79-1146, KU-1) and/or FECV Type II (79-1683) (showing non-cell associated growth). These MAbs also reacted with CCV or TGEV. These MAbs recognized peplomer (E2) glycoprotein, and many antigenic differences were found in this E2 protein. These results suggest that FIPV Type II and FECV Type II viruses are antigenically closer to TGEV or CCV than to FIPV Type I viruses. Furthermore, the MAb prepared in this study has enabled discrimination between FIPV strain 79-1146 and FECV strain 79-1683, which was thought to be impossible by the previous serological method. 相似文献
24.
Rilner Alves Flores Everton Martins Arruda Jonas Pereira de Souza Junior Renato de Mello Prado Ana Carla Alves dos Santos Amanda Sasamoto Aragão 《Journal of plant nutrition》2019,42(2):137-144
Silicon (Si) is important as it increases plant tolerance to abiotic stresses. However, studies on in foliar fertilization are still scarce. The objective of this research was to evaluate the effect of foliar application of silicon on nutrition and production of Helianthus annuus. The study was carried with a completely randomized design, consisting of five doses of silicon (0; 0.84; 1.68; 2.52 and 3.36?mg L?1) applied as silicate, with four replicates. Biometric evaluations and relative chlorophyll index (RCI) measurements were performed, being also observed the dry matter production and the silicon use efficiency by plants. The RCI is not affected by the foliar application of Si, while the height and leaf area increase by 8.3 and 25.4%, respectively. Foliar Si application up to the dose of 3.36?g L?1 promotes linear increases in the dry matter production of shoots (32%), capitulum (19%) and the whole plant of sunflower (27%). 相似文献
25.
Shigemi Honma 《Scientia Horticulturae》1977,7(3):207-211
Several types of grafts were used. The cleft graft was found to be the most efficient and successful. The union was strong and the plants grew normally in the field. Indolebutyric acid (100 p.p.m.) hastened callus formation and graft union. The effect of resistant root stock on the scion is discussed. 相似文献
26.
Kawashima C Sakaguchi M Suzuki T Sasamoto Y Takahashi Y Matsui M Miyamoto A 《The Journal of reproduction and development》2007,53(1):113-120
Metabolic hormones affect ovarian function in the cow. However, the relationship between metabolic factors and ovarian function is not clear in the postpartum primiparous cow because they are still growing. The aim of the present study was to investigate in detail the time-dependent profile of the metabolic hormones, metabolites, and milk yields of ovulatory and anovulatory primiparous cows during the first follicular wave postpartum. We used 16 primiparous Holstein cows and obtained blood samples for the profiles of metabolites (glucose; non-esterified fatty acid, NEFA; ketone body; total cholesterol; and aspartate aminotransferase), metabolic hormones (growth hormone, GH; insulin-like growth factor-I, IGF-1; and insulin), and progesterone every other day from 1 to 21 days postpartum. In addition, all ovaries were observed using ultrasound. Dairy milk yield was recorded during the experimental period. In all cows, the first follicular wave postpartum was observed and 6 of the cows ovulated. The plasma glucose (P<0.0001) and IGF-1 (P<0.001) concentrations were lower and the plasma NEFA (P<0.0001) and ketone bodies (P<0.0001) concentrations and daily milk yield (P<0.0001) were higher in the anovulatory cows compared to the ovulatory cows. However, the GH levels, which enhance lipolysis for milk production, insulin and other metabolites did not differ between the two groups. In conclusion, the present study suggests that anovulation of the dominant follicle during the first follicular wave postpartum in primiparous cows is induced by low IGF-1 levels that are similar to those of multiparous cows. In addition, anovulatory cows are likely to mobilize body fat stores for milk production more easily than ovulatory cows. 相似文献
27.
Success in obtaining an interspecific hybrid between B. pekinensis and B. oleracea var. acephala was possible through the use of the Chinese cabbage as the seed parent and the use of the growth regulator, N-m-tolylphthalamic acid. The hybrids appeared vegetatively intermediate between the parents except for the inflorescence which appeared more like the kale parent. The F1 was backcrossed with ease to the Chinese cabbage parent using the hybrid either as the pollen or seed parent. No backcross was made to the kale parent. Segregation in the F2 was continuous for most characters studied.Michigan Agricultural Experiment Station, Journal Article Number 2588. 相似文献
28.
Katayama S Shigemi K Cline MA Furuse M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):471-474
We previously demonstrated that intracerebroventricular (ICV) injection of orexin-A induces arousal and increased metabolic turnover rates of norepinephrine, dopamine, and serotonin in layer (egg-type) chicks. Because monoamine oxidase-A (MAO-A) is a potent degrading enzyme of these monoamines, we hypothesized that orexin-A may mediate its arousal-inducing effects through MAO-A. Therefore, we simultaneously injected clorgyline, a specific inhibitor of MAO-A, with orexin-A and examined behavior of chicks. Behaviors associated with arousal were attenuated in the group of chicks that received clorgyline and orexin-A compared with those that received orexin-A alone. For the monoamine turnover rate, enhancement of the turnover rate of serotonin by orexin-A was attenuated by clorgyline. Therefore, we conclude that orexin-A-induced arousal is dependent upon monoamine neural activities stimulated by MAO-A in chicks. 相似文献
29.
Akifumi Mizuno Takanori Tsukamoto Yoshiaki Shimizu Hitoshi Ooya Takayuki Matsuura Norihiko Saito Shigeyoshi Sato Shigemi Kikuchi Tsuneyasu Uzuki Kouji Azegami 《Journal of General Plant Pathology》2010,76(1):43-51
Black lesions on shoots of European pear trees observed in an orchard in Yamagata Prefecture in May 2007 were suspected to
be caused by a bacterial pathogen. The surface of the colonies isolated on a high sucrose medium did not have the crater morphology
that is characteristic of E. amylovora bvs. 1–3, and a specific DNA fragment was amplified from the isolates in the PCR using the EprpoD primer set. The partial
sequences of the 16S rRNA gene placed the isolates in the genus Erwinia. The isolates differed serologically from E. amylovora biovars and E. pyrifoliae in an Ouchterlony double-diffusion test although their bacterial properties suggested that they are closely related to E. amylovora biovars and E. pyrifoliae. In a DNA–DNA hybridization test, the relatedness between the isolates and E. amylovora biovars or E. pyrifoliae did not exceed 70% level, indicating that they are independent species. Thus, the isolates belongs to the genus Erwnia but are not E. amylovra or E. pyrifoliae. After succulent pear shoots were injected with bacterial suspensions (109, 108, 107 and 106 cfu/ml) of the isolates, lesions formed with 109 and 108 cfu/ml, but the disease incidence with 108 cfu/ml was much lower than with E. amylovora and E. pyrifoliae. Virulence of the present isolates is thus thought to be very weak. On the basis of these results, we consider that this
is a new shoot disease of European pear. In the 2007 season, all affected trees were pulled out after harvest. No symptoms
have been observed in field surveys since the fruitlet season in 2007. 相似文献
30.
Sasamoto Y Sakaguchi M Katagiri S Yamada Y Takahashi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1083-1086
The effects of twisting and type (single- or double-lumen) of aspiration needle on the efficiency of transvaginal ultrasound-guided ovum pick-up (US-guided OPU) were investigated in cattle. The first study using slaughterhouse ovaries revealed that twisting of the needle during follicle aspiration improved the oocyte recovery rate without deleterious effects on the attachment of cumulus layers. Vacuum pressure affected the oocyte recovery and cumulus attachment, regardless of the needle type. The needle type did not affect the oocyte recovery or cumulus attachment with an optimized vacuum pressure. In the second study, US-guided OPU was performed in live cows using two types of needles with a vacuum pressure of 75 mmHg. The needle type did not affect the oocyte recovery or cumulus attachment of the recovered oocytes. The results revealed that twisting of the needle is effective in follicle aspiration, and suggested that a single-lumen needle is as useful as a double-lumen needle for US-guided OPU in cattle. 相似文献