Pathological findings of Japanese Black Cattle living in the restricted area of the Fukushima Daiichi Nuclear Power Plant accident, 2013–2016          下载免费PDF全文

Jun Sasaki  Kayoko Hiratani  Itaru Sato  Hiroshi Satoh  Yoshitaka Deguchi  Hiroyuki Chida  Masahiro Natsuhori  Takahisa Murata  Kenji Ochiai  Kumiko Otani  Keiji Okada  Nobuhiko Ito 《Animal Science Journal》2017,88(12):2084-2089

Fifty‐one Japanese black cattle from four farms in the evacuation zone of the Fukushima nuclear accident were examined pathologically during the period, 2013–2016. We found no evidence of a radiation effect on pathological findings in any of these autopsy cases, although nine cases (3.7%) of enzootic bovine leukosis (EBL) and three cases (1.2%) of goiter were diagnosed. Estimated integrating dose of external exposure in EBL cases ranged from a maximum of 1200 mSv to a minimum of 72 mSv. Clinically, five cases showed wobble, dysstasia or paralysis. Exophthalmos was observed in three cases. Macroscopically, enlarged lymph nodes, multiple irregular masses of intra‐abdominal and intrapelvic adipose tissue, diffuse thickening of the abomasal submucosa with ulceration, and numerous white nodules of myocardium were observed. Histologically, neoplastic lymphoid cells were extensively proliferated and infiltrated in the lesions. Lymphoid tumor cells showed mature or large lymphoblastic appearance. Immunohistochemically, tumor cells were positive for BLA‐36, CD20 and CD5, and negative for CD3. Three cases showed diffuse enlargement of the thyroid gland with no mass or nodule lesion. Histologically, diffuse proliferation of follicular epithelium was observed with preservation of normal structures. There were no malignant findings such as cellular atypia or invasion to capsule.  相似文献   

Epigenetic modulation on cat‐cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A          下载免费PDF全文

Manita Wittayarat  Yoko Sato  Lanh Thi Kim Do  Kaywalee Chatdarong  Theerawat Tharasanit  Mongkol Techakumphu  Masayasu Taniguchi  Takeshige Otoi 《Animal Science Journal》2017,88(4):593-601

This study aimed to determine the acetylation at lysine 9/18/23 of histone H3 (H3K9ac/H3K18ac/H3K23ac; H3K9/18/23 ac) and the di‐methylation at lysine 9 of histone H3 (H3K9me2) during early embryogenesis among trichostatin A (TSA)‐treated interspecies somatic cell nuclear transfer (iSCNT) cat‐cow (TSA‐iSCNT) embryos, TSA‐untreated iSCNT cat‐cow control (control) embryos and bovine in vitro fertilization (IVF) embryos, because TSA‐iSCNT embryos can develop to blastocysts. Compared to the control embryos, higher expressions of H3K9/18/23 ac were observed in TSA‐iSCNT embryos and IVF embryos at most following stages (2 h post‐fusion / post‐insemination (PF/PI) to eight‐cell stage). At 6 h PF/PI the expression of H3K9/23 ac in TSA‐iSCNT embryos and IVF embryos were lower than those in control embryos, and the expression of H3K18ac was no difference among the three groups. The expression of H3K9/23 ac increased in TSA‐iSCNT embryos and IVF embryos at pronuclear (PN) stages. The expression of H3K9me2 in TSA‐iSCNT embryos resembled that of IVF embryos at 2 h PF/PI to PN stages, and these expression levels were greater than those of control embryos. These results suggest that treatment of iSCNT embryos with TSA modifies the patterns of histone modification at certain lysine residues in a manner that is comparable with that seen in IVF during early embryogenesis.  相似文献   

连续14年保护性耕作对土壤总有机碳和轻组有机碳的影响   总被引：1，自引：0，他引：1  

刘杰  李玲玲  谢军红  邓超超  彭正凯  Yeboah Stephen  Lamptey Shirley 《干旱地区农业研究》2017,35(1):8-13

依托于2001年布设在陇中黄土高原半干旱雨养农业区的保护性耕作定位试验,于2014年测定了5种保护性耕作(免耕+秸秆覆盖NTS、免耕NT、传统耕作+秸秆翻埋TS、传统耕作+地膜覆盖TP和免耕+地膜覆盖NTP)和传统耕作T处理下小麦-豌豆双序列轮作中表层土壤(0~5、5~10、10~30 cm)总有机碳(SOC)和轻组有机碳(LFOC)在作物生育期前后的变化。结果表明:土壤总有机碳和轻组有机碳在土壤剖面上均随着土层深度的增加而降低;相比传统耕作T,NTS和TS处理能显著提高0~30 cm土层中SOC、LFOC的含量,在作物播种前较T分别提高了19.51%、64.58%和13.36%、42.08%,在收获后分别提高了28.00%、85.37%和18.61%、77.82%,而SOC、LFOC含量NT和TP处理与T处理间差异不显著;从作物播种前至收获后,各处理下0~30 cm土层SOC含量均有减小趋势,其中NTS和TS处理变化量最小,NT和TP处理加大了作物生育期间SOC和LFOC的消耗;LFOC可以灵敏地反应出土壤有机碳的变化。因此,在该区推行以免耕、秸秆覆盖为主的保护性耕作措施更有利于碳的积累和土壤质量的改善,促进该区农业的可持续发展。  相似文献   

Uptake and translocation of nitrogen in patumma (Curcuma alismatifolia) by leaves or root     

Chamaiporn Anuwong  Takuji Ohyama  Kuni Sueyoshi  Norikuni Ohtake  Takashi Sato 《Journal of plant nutrition》2017,40(8):1204-1212

An adequate supply of nitrogen (N) is important for patumma growth and flower quality. This study aimed to compare the uptake and translocation of N by foliar and root application. Fertilization with ¹⁵ nitrate (NO₃)-N via roots or leaves was carried out at four stages, at the 1st to 4th fully expanded leaf (FEL) stages, and the plants were sampled at each successive stage. The uptake and translocation of ¹⁵N from foliar or root applications showed relatively similar patterns at all stages. Although the N fertilizer utilization rate by roots was higher than that via leaves, the foliar application stimulated reproductive growth by earlier flowering. The N supplied at the 1st FEL and the 2nd FEL was utilized mainly in leaves, whereas supplying N at the 3rd and 4th FEL promoted flower quality. Fertilizer application method and stage of application influence the utilization rate and translocation of N to the sink organs.  相似文献   

<Emphasis Type="Italic">Arcobacter</Emphasis> spp. in fecal samples from Brazilian farmed caimans (<Emphasis Type="Italic">Caiman yacare</Emphasis>, Daudin 1802)     

Maria Gabriela Xavier Oliveira  Leandro Nogueira Pressinotti  Giovane Spínola Carvalho  Mirela Caroline Vilela Oliveira  Luisa Zanolli Moreno  Carlos Emilio Cabrera Matajira  Alessandro Spínola Bergamo  Victor Manuel Aleixo  Alexandre Caixeta Veiga  Elvis de Souza Corsino  Ana Paula Guarnieri Christ  Maria Inês Zanolli Sato  Andrea Micke Moreno  Terezinha Knöbl 《Tropical animal health and production》2017,49(4):777-782

The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans.  相似文献   

Evaluation of the prognostic significance of BCL6 gene expression in canine high-grade B-cell lymphoma     

Sato M  Kanemoto H  Kagawa Y  Kobayashi T  Goto-Koshino Y  Mochizuki H  Takahashi M  Fujino Y  Ohno K  Tsujimoto H 《Veterinary journal (London, England : 1997)》2012,191(1):108-114

The clinical usefulness of BCL6 gene expression was evaluated as a prognostic indicator in dogs with high-grade B-cell lymphoma. Forty-four dogs were diagnosed with centroblastic or B-cell immunoblastic type lymphoma according to the updated Kiel classification. BCL6 mRNA expression was measured by real-time PCR and its relationship with prognosis was analyzed. Progression-free and overall survival was not significantly different between the high BCL6 expression group (higher than the median) and the low BCL6 expression group (lower than the median) (P=0.99 and P=0.61, respectively). No correlation between BCL6 and prognosis was observed in this study, which is inconsistent with findings reported for human diffuse large B-cell lymphoma. BCL6 protein expression was not detected in the 11 dogs evaluated by immunohistochemistry. Furthermore, BCL6 protein expression was assessed in 13 archived paraffin-embedded high-grade canine lymphoma tissues and all were also negative. The results suggest that most canine high-grade B-cell lymphomas correspond to human diffuse large B-cell lymphoma with no immunohistochemical expression of BCL6.  相似文献   

Spinal cord trauma in a cat caused by ingestion of a splinter     

Tanaka T  Akiyoshi H  Shimazaki H  Sato A  Inada M  Kiyomiya K  Shimizu J  Cao Z  Li Y  Hayashi A  Sone K  Kitai T  Ohashi F 《Journal of Feline Medicine and Surgery》2012,14(2):155-157

A 5-month-old male Abyssinian presented with right hemiplegia. Cervical radiographs, haematology and serum biochemistry were normal. A foreign body was identified on computed tomography (CT) and magnetic resonance imaging (MRI). Removal of the foreign body was carried out via a dorsal approach between the occipital bone and the C1 vertebral body using intra-operative CT. No complications were encountered. Thirty days after surgery, the cat was ambulatory.  相似文献   

Anthracnose of bacopa caused by Colletotrichum destructivum     

Keisuke Tomioka  Toyozo Sato  Jouji Moriwaki  Yuichi Terasawa  Hiroki Koganezawa 《Journal of General Plant Pathology》2012,78(2):133-135

Severe spotting and blighting of leaves were found on bacopa (Sutera cordata), a scrophulariaceous ornamental, in greenhouses in Gunma Prefecture, Japan, from January through February 2007. After we isolated and identified the causal fungus as Colletotrichum destructivum and inoculated host plants with the isolate to confirm pathogenicity, we named this new disease anthracnose of bacopa.  相似文献   

Science and society. Rebuilding public trust in science for policy-making     

T Arimoto  Y Sato 《Science (New York, N.Y.)》2012,337(6099):1176-1177

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A case of congenital hyperostosis in a newborn piglet     

Uetsuka K  Suzuki T  Ogawa H  Sato H  Doi K  Nunoya T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(2):259-262

One female newborn piglet showed prominent thickening of both forelimbs and died soon after birth. Histopathologically, thin and woven trabeculae of bone was extending out from the edge of cortical bone in the affected forelimbs, and diagnosed as congenital hyperostosis. The extent of radially proliferated trabeculae was most prominent in radioulna. Many round- to spindle-shaped cells were observed in periosteum, which were considered to be osteoblasts. Around the periosteum, the mesenchymal proliferation was extensive with abundant mucus, and cartilaginous metaplastic changes were observed mainly around the radioulna and humerus. Dilatation of vessels with fibrin deposition in vessel walls was often observed, which were considered to reflect the localized circulatory disturbance.  相似文献