Physiological response,blood chemistry profile and mucus secretion of red sea bream (<Emphasis Type="Italic">Pagrus major</Emphasis>) fed diets supplemented with <Emphasis Type="Italic">Lactobacillus rhamnosus</Emphasis> under low salinity stress     

Mahmoud A. O. Dawood  Shunsuke Koshio  Manabu Ishikawa  Mabrouk El-Sabagh  Saichiro Yokoyama  Wei-Long Wang  Zhang Yukun  Adissin Olivier 《Fish physiology and biochemistry》2017,43(1):179-192

Environmental stressors caused by inadequate aquaculture management strategies suppress the immune response of fish and make them more susceptible to diseases. Therefore, efforts have been made to relieve stress in fish by using various functional feed additives in the diet, including probiotics. The present work evaluates the effects of Lactobacillus rhamnosus (LR) on physiological stress response, blood chemistry and mucus secretion of red sea bream (Pagrus major) under low salinity stress. Fish were fed four diets supplemented with LR at [0 (LR0), 1 × 10² (LR1), 1 × 10⁴ (LR2) and 1 × 10⁶ (LR3) cells g^?1] for 56 days. Before stress, blood cortisol, urea nitrogen (BUN) and total bilirubin (T-BIL) showed no significant difference (P > 0.05), whereas plasma glucose and triglyceride (TG) of fish-fed LR2 and LR3 diets were significantly lower (P < 0.05) than those of the other groups. Plasma total cholesterol (T-CHO) of fish-fed LR3 diet was significantly (P < 0.05) lower than that of the other groups. Furthermore, total plasma protein, mucus myeloperoxidase activity and the amount of mucus secretion were significantly enhanced in LR-supplemented groups when compared with the control group (P < 0.05). After the application of the low salinity stress test, plasma cortisol, glucose, T-CHO and TG contents in all groups showed an increased trend significantly (P < 0.01) compared to the fish before the stress challenge. However, plasma total protein and the amount of secreted mucus showed a decreased trend in all groups. On the other hand, BUN, T-BIL and mucus myeloperoxidase activity showed no significant difference after exposure to the low salinity stress (P > 0.05). In addition, the fish that received LR-supplemented diets showed significantly higher tolerance against low salinity stress than the fish-fed LR-free diet (P < 0.05). The physiological status and the detected immune responses, including total plasma protein and mucus myeloperoxidase activity in red sea bream, will provide a more comprehensive outlook of the effects of probiotics to relieve stress in fish.  相似文献   

Dietary value of gametophytes and juvenile sporophytes of the brown macroalga <Emphasis Type="Italic">Eisenia bicyclis</Emphasis> for juvenile abalone <Emphasis Type="Italic">Haliotis diversicolor</Emphasis>     

Toshihiro Onitsuka  Tomohiko Kawamura  Shunsuke Iwanaga  Satoshi Ohashi  Toyomitsu Horii 《Fisheries Science》2010,76(4):619-623

The dietary value of juvenile stages (gametophyte and juvenile sporophyte) of the brown macroalga Eisenia bicyclis for post-larval and juvenile abalone Haliotis diversicolor of 2.0–6.5 mm in shell length (SL) was examined and compared with that of a benthic diatom, Nitzschia sp., in laboratory experiments. Most abalone actively fed on these diets, but there were large variations in the growth rate among the diets and among the growth stages of abalone. Growth rates of abalone fed on Nitzschia sp. were highly variable within each growth stage, but showed no clear differences among growth stages. In contrast, in abalone fed gametophytes or juvenile sporophytes, growth rates linearly increased as abalone grew. Growth rates of >60 μm SL/day were observed in juveniles of >3 mm SL fed gametophytes, and juveniles of >5 mm SL fed juvenile sporophytes. These results indicate that the dietary value of the juvenile stages of E. bicyclis for the abalone changes as they grow, and with growth juvenile abalone begin to efficiently utilize gametophytes and juvenile sporophytes in that order.  相似文献   

Growth stimulation of juvenile abalone <Emphasis Type="Italic">Haliotis discus hannai</Emphasis> by feeding with salmon growth hormone in sodium alginate gel     

Shunsuke Moriyama  Suehiro Furukawa  Hiroshi Kawauchi 《Fisheries Science》2009,75(3):689-695

We examined the ability of salmon growth hormone (sGH) in sodium alginate (SA) gel to accelerate the somatic growth of juvenile abalone, Haliotis discus hannai, by feeding. After the feeding of sGH at 50 μg or 100 μg in 350 mg SA gel, immunoreactivity to sGH in body fluid was maximal after 12 h, and was still detectable at 24 h. No immunoreactivity was observed in the control group. Following the feeding of sGH at 0.5 mg or 5 mg/8 g of SA gel at 7- and 14-day intervals, there was a greater increase in shell length and body weight than in the control. In abalone, sGH can be transported from food into the circulatory system and subsequently improve somatic growth.  相似文献   

Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen     

Inwoo Bae  Kiyoshi Osatomi  Asami Yoshida  Atsuko Yamaguchi  Katsuyasu Tachibana  Tatsuya Oda  Kenji Hara 《Fisheries Science》2009,75(3):765-770

A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25–37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T _m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.  相似文献   

Replacement of squid and krill meal by snail meal (Buccinum striatissimum) in practical diets for juvenile of kuruma shrimp (Marsupenaeus japonicus)          下载免费PDF全文

Amina S Moss  Shunsuke Koshio  Manabu Ishikawa  Saichiro Yokoyama  Truong H Nhu  Mahmoud A O Dawood  Weilong Wang 《Aquaculture Research》2018,49(9):3097-3106

A 60‐day feeding trial was conducted to investigate the effects of substituting squid and krill meal with marine snails (Buccinum striatissimum) into the diets of juvenile kuruma shrimps (Marsupenaeus japonicus). Five experimental diets were formulated to contain varying levels of snail meal at 0%, 25%, 50%, 75% and 100% (D1, D2, D3, D4 and D5, respectively) and fed to juvenile kuruma shrimps (initial mean weight 0.27 ± 0.02 g). The results showed that weight gain, feed intake and specific growth rate were improved significantly in D4 and D5 groups when compared with D1 group (p < 0.05). Significant differences were not detected in survival rate among all shrimps fed diets containing several levels of snail meal (p > 0.05). Crude protein content of shrimps fed the control diet was significantly lower than other treatments (p < 0.05). Lipid content in shrimps fed 50% snail meal were significantly higher than the control while cholesterol content in shrimps fed 100% snail meal were significantly decreased and polyunsaturated fatty acid contents were significantly increased in shrimps fed 75%–100% snail meal (p < 0.05). These results suggest that supplementing snail meal for complete replacement of squid and krill meal can be done to improve juvenile kuruma shrimps’ growth and reducing their cholesterol levels.  相似文献   

High-yield SRI in West Java by decomposing straw in waterlogged paddy field     

Masato Oda  Kazunobu Toriyama  Shigeki Yokoyama 《Paddy and Water Environment》2018,16(4):887-891

System of Rice Intensification (SRI) often achieved higher yield than conventional practice. We identified the high-yielding farmers from the yield records of 1909 paddy fields belonging to an organic farmers’ association. Farmers whose yields were from 8.4 to 10.4 t ha^?1 were interviewed and their fields surveyed. Their yields had increased by an estimated average of 40% following the adoption of SRI practices. They applied 2–12 t ha^?1 of compost. Compared to the conventional practice, they shortened seedling age at transplanting from 27.4 to 17.6 days and reduced the number of seedlings per hill from 4–6 to 2–3, while hill spacing remained unchanged. Instead of intermittent irrigation which is recommended in standard SRI, they kept shallow flooding of 1–2 cm. Although they applied a lot of compost, no correlation was found between the amount of compost application and the yields. Instead, high-yielding farmers returned rice straw into waterlogged paddy after harvest, which presumably is an ideal condition for biological nitrogen fixation. This may occur around rice straw during decomposition under waterlogged condition and might supplement the negative nitrogen balance, thereby enabling the high yield as compared with conventional practices where the fresh rice straw is removed and/or burned.  相似文献   

Effect of replacing fishmeal with canola meal on growth and nutrient utilization in kuruma shrimp Marsupenaeus japonicus (Bate)     

Mahbuba Bulbul  Md. Abdul Kader  Shunsuke Koshio  Manabu Ishikawa  Saichiro Yokoyama 《Aquaculture Research》2014,45(5):848-858

A feeding trial was conducted to assess the nutritional values of canola meal as a substitute for fishmeal in diets for kuruma shrimp using five isocaloric diets (190 kJ kg^?1) prepared by replacing 0%, 10%, 20%, 30% and 40% of fishmeal protein with canola meal protein in CM0, CM10, CM20, CM30 and CM40 diets respectively. Triplicate groups of juveniles (0.19 g) were fed the respective diets for 60 days in tanks. At the end of trial, weight gain (%) and specific growth rate (% day^?1) were not significantly (P > 0.05) different among shrimp fed CM0, CM10 and CM20 diets. However, growth was significantly (P < 0.05) decreased in shrimp fed CM30 and CM40 diets. As with growth performance, feed intake, feed conversion ratio and protein efficiency ratio were also decreased (P < 0.05) in CM30 and CM40 groups. Whole body dry matter, methionine and proline were significantly decreased with canola meal substitution levels. In general, retention efficiency of protein and indispensible amino acids were decreased as canola meal increased in diets. It may be concluded that 20% fishmeal protein can be successfully replaced with canola meal, while supplementation of amino acids or blending with complementary proteins could facilitate higher fishmeal replacements in kuruma shrimp diets.  相似文献   

Effect of glimepiride and nateglinide on serum insulin and glucose concentration in healthy cats     

A. Mori  P. Lee  T. Yamashita  Y. Nishimaki  H. Oda  K. Saeki  Y. Miki  H. Mizutani  K. Ishioka  T. Honjo  T. Arai  T. Sako 《Veterinary research communications》2009,33(8):957-970

Glimepiride and nateglinide are two common oral hypoglycemic agents currently being used with humans suffering from Type 2 diabetes mellitus. Neither drug has been tested with cats thus far and it is currently unknown whether either of these drugs exert any effect in cats or not. The objective of this study was to determine the effect of glimepiride and nateglinide on glucose and insulin responses in healthy control cats, in order to determine their potential use in diabetic cats. The intravenous glucose tolerance tests was carried out since it is an excellent test for evaluating pancreatic β-cell function for insulin secretion. Alterations in the insulin secretion pattern can be perceived as the earliest sign of β-cell dysfunction in many species, including cats. Nateglinide demonstrated a quick action/short duration type effect with serum glucose nadiring and insulin response peaking at 60 and 20 minutes, respectively. Alternatively, glimepiride is medium-to-long acting with serum glucose nadiring and insulin response peaking at 180 minutes and 60 minutes, respectively. Nateglinide’s potency was evident allowing it to induce a 1.5–2 higher preliminary insulin peak (3.7?±?1.1 pg/ml) than glimepiride’s (2.5?±?0.1 pg/ml), albeit only for a short period of time. Because glimepiride and nateglinide have a shared mode of action, no significant differences in overall glucose AUC_0-360min (24,435?±?2,940 versus 24,782?±?2,354 mg min/dl) and insulin AUC_0-360min (410?±?192 versus 460?±?159) in healthy control cats were observed. These findings may provide useful information when choosing a hypoglycemic drug suited for the treatment of diabetic cats depending on the degree of diabetes mellitus the cat is suffering from.  相似文献   

Identification of a peptide in enzymatic hydrolyzate of cheese that inhibits ovalbumin permeation in Caco-2 cells     

Tanabe S  Isobe N  Miyauchi E  Kobayashi S  Suzuki M  Oda M 《Journal of agricultural and food chemistry》2006,54(18):6904-6908

Because the first step in the triggering of food allergy is the permeation of the allergen through the intestine, enhancement of the intestinal barrier function is thought to be effective for preventing food allergy. In this study, a peptide that inhibits ovalbumin (OVA) permeation in an in vitro Caco-2 cell model was isolated from enzymatic hydrolyzate of cheese (EHC). Amino acid sequence analysis identified the active peptide as GPIVLNPWDQ, a sequence identical to amino acids 102-111 of alphas2-casein. The decapeptide significantly inhibited OVA permeation at a concentration of 10(-6) M. In addition, it was found that a pentapeptide half, NPWDQ, is essential for the inhibitory activity because NPWDQ but not GPIVL had nearly the same inhibitory activity as GPIVLNPWDQ. The possibility exists that EHC and/or peptides possessing the NPWDQ sequence can be practically applied to the prevention of food allergy.  相似文献   

Effect of feeding rotifers enriched with taurine on the growth and survival of larval amberjack Seriola dumerili     

Hiroyuki Matsunari  Hiroshi Hashimoto  Takashi Iwasaki  Kentaro Oda  Yoshitsugu Masuda  Hitoshi Imaizumi  Kazuhisa Teruya  Hirofumi Furuita  Takeshi Yamamoto  Kazuhisa Hamada  Keiichi Mushiake 《Fisheries Science》2013,79(5):815-821

The effect of feeding rotifers enriched with taurine on the growth performance and survival of larval amberjack Seriola dumerili was investigated. Rotifers were enriched with a commercial taurine supplement at four levels (0, 200, 400, and 800 mg/l). The larvae were fed the enriched rotifers in triplicate from 3 days post-hatch for 7 days under static conditions. The average taurine contents of the rotifers were 1.5, 2.7, 4.2, and 7.2 mg/g dry matter, respectively. The growth of the fish fed rotifers enriched with the taurine supplement at 800 mg/l was significantly (P < 0.05) improved compared with that of the fish fed the rotifers without taurine enrichment. The survival rate improved proportionally up to a taurine supplement level of 400 mg/l, but no significant differences in survival were observed among treatments. The fraction of the larvae with inflated swim bladders did not vary significantly between treatments. Taurine content in the whole fish body increased with the taurine level in the rotifers. These results suggest that taurine enrichment of rotifers is an effective method of enhancing the growth of amberjack larvae.  相似文献