Involvement of Bacillus licheniformis and Micrococcus kristinae During Ripening of Salt Fermented Indian Shad (Tenualosa ilisha)     

Ranendra Kumar Majumdar  Binaya Bhusan Nayak  Subrata Basu 《Journal Of Aquatic Food Product Technology》2013,22(4):423-440

ABSTRACT

“Lona ilish” is a salt fermented fish product prepared exclusively from fatty Indian shad (Tenualosa ilisha). Despite the presence of salt and metals coming from the container used for fermentation, rancidity is not observed as long as the products are kept immersed in the fermenting brine. To understand the technical principles of this indigenous preservation method, the traditional preparation was followed along with analyses of chemical and microbiological changes. The biochemical and microbiological changes were followed at 15 day intervals during the fermentation period of 150 days. From the 45th day of fermentation onwards, the microbial flora in the product was composed of only two species, tentatively identified as Bacillus licheniformis and Micrococcus kristinae. Because Bacillus licheniformis showed some atypical biochemical reactions, it was tentatively identified as Bacillus licheniformis var. III. These two bacterial species either singly or collectively were involved in the fermentation process.  相似文献   

Using contingent valuation to estimate the willingness of tourists to pay for urban forests: A study in Savannah, Georgia     

Suman Majumdar  Jinyang Deng  Yaoqi Zhang  Chad Pierskalla 《Urban Forestry & Urban Greening》2011,10(4):275-280

This study estimated the monetary value of urban forests’ non-priced benefits to tourists. Data collected by a face-to-face self-administered survey of urban tourists in Savannah, Georgia, USA were used to estimate tourists’ willingness to pay (WTP) for urban forests by the contingent valuation method. Individual WTP was found higher among tourists with graduate school education. Results suggested that WTP for urban forests also increased significantly with income and destination loyalty of the tourists. Estimated mean and median WTP values were $11.25 (95% confidence interval: $7.34, $15.16) and $2.10 (95% confidence interval: $1.38, $2.82), respectively. Based on the estimated mean WTP, annual value of urban forests to tourists in Savanna in 2009 ranged from a minimum of $81 million to a maximum of $167 million with a 95% confidence interval. The annual value was $11.55 million (95% confidence interval: $7.59 million, $15.51 million) based on the estimated median WTP and assuming at least 50% of the tourists in Savannah would pay the median amount. As the mean was greatly influenced by extreme WTP values in the data, the annual value based on the median value was a more conservative estimate.  相似文献   

Influence of feeding habits of fish on the characteristics of their visceral alkaline proteases with special reference to detergent compatibility     

Biswajit Mohanty  Upasana Mohanty  Ranendra K. Majumdar 《Aquaculture Research》2021,52(1):364-372

Technical characteristics and detergent compatibility of visceral alkaline proteases of three freshwater fish, namely Labeo rohita, Pangasianodon hypophthalmus and Cyprinus carpio of different feeding habits, were studied. Crude enzyme extract was partially purified by (NH₄)₂SO₄ precipitation and dialysis. The molecular weight was in the range of 20–63 kDa. The enzyme purification folds post‐dialysis were found to be 1.55, 1.81 and 2.17 in case of Rohu, Pangas and Common carp respectively. The alkaline protease from Rohu, Pangas and Common carp exhibited maximum activity at pH 10.0, 9.0 and 11.0 respectively. The enzyme temperature optima observed were 60°C (Rohu and Pangas) and 70°C (Common carp). SBTI and EDTA inhibited more than 90% of the activity at conc. of 50 mM. Exposure of the proteases to non‐ionic surfactants like Tween 20–80 retained about 92%–100% and 76%–100% of their activity at conc. (v/v) of 1% and 5% respectively. Proteases were found less stable in the presence of SDS. There was moderate to lesser influence of H₂O₂ and sodium perborate on the proteolytic activity. The alkaline protease from omnivorous fish was found superior compared to the herbivore and carnivore in respect of pH and temperature optima and stability with detergents and oxidizing agents.  相似文献   

Genetic diversity among sea otter isolates of Toxoplasma gondii     

Sundar N  Cole RA  Thomas NJ  Majumdar D  Dubey JP  Su C 《Veterinary parasitology》2008,151(2-4):125-132

Sea otters (Enhydra lutris) have been reported to become infected with Toxoplasma gondii and at times succumb to clinical disease. Here, we determined genotypes of 39 T. gondii isolates from 37 sea otters in two geographically distant locations (25 from California and 12 from Washington). Six genotypes were identified using 10 PCR-RFLP genetic markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico, and by DNA sequencing of loci SAG1 and GRA6 in 13 isolates. Of these 39 isolates, 13 (33%) were clonal Type II which can be further divided into two groups at the locus Apico. Two of the 39 isolates had Type II alleles at all loci except a Type I allele at locus L358. One isolate had Type II alleles at all loci except the Type I alleles at loci L358 and Apico. One isolate had Type III alleles at all loci except Type II alleles at SAG2 and Apico. Two sea otter isolates had a mixed infection. Twenty-one (54%) isolates had an unique allele at SAG1 locus. Further genotyping or DNA sequence analysis for 18 of these 21 isolates at loci SAG1 and GRA6 revealed that there were two different genotypes, including the previously identified Type X (four isolates) and a new genotype named Type A (14 isolates). The results from this study suggest that the sea otter isolates are genetically diverse.  相似文献   

Epithelial metaplasia in experimental vitamin A deficient goats     

B Dutt  B N Majumdar 《The Indian veterinary journal》1969,46(9):789-791

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Effect of supplementation of vitamin E, selenium and their different combinations on the performance and immune response of broilers     

Swain BK  Johri TS  Majumdar S 《British poultry science》2000,41(3):287-292

1. The effect of dietary vitamin E, selenium (Se) and their different combinations on body weight gain, food consumption, food conversion efficiency, leukocyte migration inhibition and antibody production was determined in broilers. 2. Chicks were fed on maize-soya bean based diets with concentrations of supplemental vitamin E varying from 0 to 300 IU/kg and selenium concentrations varying from 0 to 1 mg/kg either alone or in combination from 1 to 42 d of age. 3. The chicks were immunised for Newcastle Disease Virus (NDV) vaccine at 21 d. Per cent leukocyte migration inhibition (LMI) was studied on 42 d. Antibodies to NDV in serum were determined at 10 and 21 d post immunisation (PI). 4. Chicks receiving Se, 1 mg/kg and vitamin E 300 IU/kg had significantly higher cellular immune responses in terms of per cent LMI. 5. Maximum body weight gain and best efficiency of food utilisation were obtained in chicks fed diets containing 0.50 mg/kg Se and 300 IU/kg vitamin E. 6. Significantly higher antibody titres (HI and ELISA) at 10 d PI were attributed to 0.06 mg/kg and 150 IU/kg Se and vitamin E, respectively. 7. These data suggest that optimum growth and immune response may be achieved at supplemental level of Se of 0.06 mg/kg and vitamin E at 150 IU/kg. The vitamin E level is higher than that recommended by NRC (1984, 1994).  相似文献   

卫星数据主成分分析估计娑罗双树生物量     

Nilanchal Patel  Arnab Majumdar 《林业研究》2010,21(4):469-474

Spatio-temporal assessment of the above ground biomass (AGB) is a cumbersome task due to the difficulties associated with the measurement of different tree parameters such as girth at breast height and height of trees. The present research was conducted in the campus of Birla Institute of Technology, Mesra, Ranchi, India, which is predominantly covered by Sal (Shorea robusta C. F. Gaertn). Two methods of regression analysis was employed to determine the potential of remote sensing parameters with the AGB measured in the field such as linear regression analysis between the AGB and the individual bands, principal components (PCs) of the bands, vegetation indices (VI), and the PCs of the VIs respectively and multiple linear regression (MLR) analysis between the AGB and all the variables in each category of data. From the linear regression analysis, it was found that only the NDVI exhibited regression coefficient value above 0.80 with the remaining parameters showing very low values. On the other hand, the MLR based analysis revealed significantly improved results as evidenced by the occurrence of very high correlation coefficient values of greater than 0.90 determined between the computed AGB from the MLR equations and field-estimated AGB thereby ascertaining their superiority in providing reliable estimates of AGB. The highest correlation coefficient of 0.99 is found with the MLR involving PCs of VIs.  相似文献   

Thermoelectricity in molecular junctions     

Reddy P  Jang SY  Segalman RA  Majumdar A 《Science (New York, N.Y.)》2007,315(5818):1568-1571

By trapping molecules between two gold electrodes with a temperature difference across them, the junction Seebeck coefficients of 1,4-benzenedithiol (BDT), 4,4'-dibenzenedithiol, and 4,4'-tribenzenedithiol in contact with gold were measured at room temperature to be +8.7 +/- 2.1 microvolts per kelvin (muV/K), +12.9 +/- 2.2 muV/K, and +14.2 +/- 3.2 muV/K, respectively (where the error is the full width half maximum of the statistical distributions). The positive sign unambiguously indicates p-type (hole) conduction in these heterojunctions, whereas the Au Fermi level position for Au-BDT-Au junctions was identified to be 1.2 eV above the highest occupied molecular orbital level of BDT. The ability to study thermoelectricity in molecular junctions provides the opportunity to address these fundamental unanswered questions about their electronic structure and to begin exploring molecular thermoelectric energy conversion.  相似文献   

Detection of pulsed gamma rays above 100 GeV from the Crab pulsar     

VERITAS Collaboration  Aliu E  Arlen T  Aune T  Beilicke M  Benbow W  Bouvier A  Bradbury SM  Buckley JH  Bugaev V  Byrum K  Cannon A  Cesarini A  Christiansen JL  Ciupik L  Collins-Hughes E  Connolly MP  Cui W  Dickherber R  Duke C  Errando M  Falcone A  Finley JP  Finnegan G  Fortson L  Furniss A  Galante N  Gall D  Gibbs K  Gillanders GH  Godambe S  Griffin S  Grube J  Guenette R  Gyuk G  Hanna D  Holder J  Huan H  Hughes G  Hui CM  Humensky TB  Imran A  Kaaret P  Karlsson N  Kertzman M  Kieda D  Krawczynski H  Krennrich F  Lang MJ 《Science (New York, N.Y.)》2011,334(6052):69-72

We report the detection of pulsed gamma rays from the Crab pulsar at energies above 100 giga-electron volts (GeV) with the Very Energetic Radiation Imaging Telescope Array System (VERITAS) array of atmospheric Cherenkov telescopes. The detection cannot be explained on the basis of current pulsar models. The photon spectrum of pulsed emission between 100 mega-electron volts and 400 GeV is described by a broken power law that is statistically preferred over a power law with an exponential cutoff. It is unlikely that the observation can be explained by invoking curvature radiation as the origin of the observed gamma rays above 100 GeV. Our findings require that these gamma rays be produced more than 10 stellar radii from the neutron star.  相似文献   

Mitigation of N2O emission from an alluvial soil by application of karanjin     

D. Majumdar  A. Dutta  S. Kumar  H. Pathak  M. Jain 《Biology and Fertility of Soils》2001,33(5):438-442

An incubation experiment was conducted to study N₂O emissions from a Typic Ustochrept, alluvial soil, fertilized with urea and urea combined with different levels of two nitrification inhibitors, viz karanjin and dicyandiamide (DCD). Karanjin [a furano-flavonoid, obtained from karanja (Pongamia glabra Vent.) seeds] and DCD were incorporated at rates of 5, 10, 15, 20 and 25% of applied urea-N (100 mg kg^-1 soil), to the soil adjusted to field capacity moisture content. The highest N₂O flux (366 µg N₂O-N kg^-1 soil day^-1) was obtained on day 1 after incubation from soil fertilized with urea without any inhibitor. The presence of the inhibitors appreciably reduced the mean N₂O flux from the urea-treated soils. The application of karanjin resulted in a higher mitigation of total N₂O-N emission (92-96%) compared to DCD (60-71%). Rates of N₂O flux ranged from 0.9 to 140 µg N₂O-N kg^-1 soil day^-1 from urea combined with different levels of the two inhibitors (coefficient of variation=24-272%). Karanjin (62-75%) was also more effective than DCD (9-42%) in inhibiting nitrification during the 30-day incubation period.  相似文献