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41.
S.P. Mazumdar D.K. Kundu A.R. Saha B. Majumdar R. Saha A.K. Singh 《Archives of Agronomy and Soil Science》2018,64(11):1595-1607
Impacts of 43-year nutrient management on carbon (C) and nutrient dynamics were studied in a rice-wheat-jute system in tropical India. Carbon dynamics was investigated on the basis of its distribution of labile (microbial biomass C, permanganate oxidizable C and water soluble C) and slow pools (Walkley Black oxidizable organic C), its indices (C pool index, lability index) and C management index (CMI) at different soil depths. Nutrient dynamics was judged by major nutrients availability, and related soil enzymatic activities. We studied seven treatments including unfertilized control, 100% NPK (N:P2O5:K2O 60:30:60), 50% NPK, 150% NPK, 100% NP, 100% N, 100% NPK + farm yard manure (10 tonnes FYM ha?1). Nutrients availability and soil enzymatic activities were significantly increased under balanced fertilization (100% NPK + FYM) over other treatments. Labile, slow and total C was also found highest in 100% NPK + FYM. Enhanced C indices under 100% NPK + FYM over 100% NPK and other treatments signified the importance of long term balanced fertilization on soil C stabilization. Lability indexes were lower at sub-soil (30-45cm). In terms of favourable nutrient dynamics and CMI, balanced application of NPK plus organics were recommend for long term sustainability of rice-wheat-jute system in tropics. 相似文献
42.
In ovo carbohydrate supplementation modulates growth and immunity‐related genes in broiler chickens
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S. K. Bhanja A. Goel N. Pandey M. Mehra S. Majumdar A. B. Mandal 《Journal of animal physiology and animal nutrition》2015,99(1):163-173
A study was undertaken to investigate the role of in ovo administrated carbohydrates on the expression pattern of growth and immune‐related genes. In ovo injections (n = 400) were carried out on the 14th day of incubation into the yolk sac/amnion of the broiler chicken embryos. Expression of growth‐related genes: chicken growth hormone (cGH), insulin‐like growth factor‐I & II (IGF‐I & II) and mucin were studied in hepatic and jejunum tissues of late‐term embryo and early post‐hatch chicks. Expression of candidate immune genes: Interleukin‐2, 6, 10 and 12 (IL‐2, IL‐6, IL‐10 and IL‐12), Tumour necrosis factor‐alpha (TNF‐α) and Interferon gamma (IFN‐γ) were studied in peripheral blood monocyte cells of in ovo‐injected and control birds following antigenic stimulation with sheep RBC (SRBC) or mitogen concanavalin A (Con‐A). Glucose injection significantly increased the expression of IGF‐II gene during embryonic period and both cGH and IGF‐II in early post‐hatch period, while ribose‐injected chicks had higher expression of IGF‐II gene during embryonic stage. Enhanced mucin gene expression was also observed in fructose‐injected chicks during embryonic age. Glucose‐injected chicks had higher expression of IL‐6 or IL‐10, while those injected with fructose or ribose had higher expression of IL‐2, IL‐12 and IFN gamma. It is concluded that in ovo supplementation of carbohydrates might help in improving the growth of late‐term embryos and chicks. In ovo glucose could modulate humoral‐related immunity, while fructose or ribose might help in improving the cellular immunity in broiler chickens. 相似文献
43.
A new technique of cotton bale management using clustering algorithm has been proposed. The method is based on the grouping cotton bales of similar kind into respective categories using k-mean square clustering algorithm. A set of 500 cotton bales were clustered into 5 categories by minimizing the total within-group squared Euclidean distance around the 5 centroids. In order to cluster bales of different categories, 8 fibre properties, viz., strength, elongation, upper half mean length, length uniformity, short fibre content, micronaire, reflectance and yellowness of each bale have been considered. Once the bales are clustered into different categories, it is possible to prepare consistent bale mix for consecutive laydowns on the basis of frequency relative picking method. 相似文献
44.
45.
K. Rajasekaran R. Majumdar C. Sickler Q. Wei J. Cary D. Bhatnagar 《Journal of Crop Improvement》2017,31(4):628-636
Screening of gene manipulation events (transgenic, mutation/genome editing, etc.) is a cost/labor-intensive and time-consuming process in plant science research. While polymerase chain reaction (PCR) is the most commonly used method for screening, the process still requires efficient DNA extraction and subsequent confirmation. However, PCR cannot predict gene expression. To screen a larger number of transgenic plants, it would be ideal to develop a quick and reliable screening procedure. We have applied a Liberty® leaf-painting method (against bar gene under 4x35S promoter) to screen transgenic maize (Zea mays L.) plants and validated the results through PCR and quantitative real-time PCR (qRT-PCR). Liberty leaf painting at 500 mg L?1a.i. was > 95% accurate in identifying transgenic events that agreed with the PCR results. Further investigation of bar gene expression in sensitive lines that were PCR positive shows very low expression of the bar gene. We have provided a simple, and rapid assay to determine the transgene expression potential of maize plants expressing the bar gene. The herbicide can be applied to a fully expanded leaf and evaluated one week after application. Green or partially green leaf blades indicate high or moderately high expression of the bar gene and a total yellowing indicates absence or extremely low expression of the bar gene in the transgenic plants. A small volume of Liberty solution is adequate to test hundreds of maize plants, and the assay is reproducible with a high frequency (> 95%) and also displays good correlation with gene expression in planta. 相似文献
46.
A. Supriya K. Raghuveer I. Swapna M. K. Rasheeda T. Kobayashi Y. Nagahama Aparna Dutta Gupta K. C. Majumdar B. Senthilkumaran 《Fish physiology and biochemistry》2005,31(2-3):267-270
In the present study, thiourea-induced thyroid hormone depletion and thyroxine (T4) ‘overdose’ were used as a strategy to understand the influence of thyroid hormones on ovarian recrudescence of juvenile
(3-months-old), immature (8-months-old) and adult (1-year-old) air-breathing catfish, Clarias gariepinus. Thiourea-induced thyroid hormone depletion in juvenile catfish impaired ovarian development, but no significant effect was
observed in immature catfish and during late stage of ovarian recrudescence of mature catfish. T4 treatment in females undergoing late stages of ovarian recrudescence induced rapid oocyte growth by promoting its early entry
into maturational phase as evident from the presence of more number of vitellogenic and post-vitellogenic follicles, decrease
in aromatse immunoreactivity and reduced estradiol–17β levels. Hence, thyroid hormones have an important role to play during early stages of ovarian development and vitellogenesis
of catfish and also indicating that thyroid has a stage dependent effect on ovary. 相似文献
47.
Transgene integration - an analysis in autotransgenic
Labeo rohita Hamilton (Pisces: Cyprinidae) 总被引:1,自引:0,他引:1
Transgenic Labeo rohita founder population was analyzed for the presence of autotransgene having histone 3 promoter and growth hormone (GH) cDNA
(LRH3-GHcDNA) or total GH gene (LRH3-GH2.8) by PCR with transgene specific primers. Transgene specific amplification was seen
with LRH3-GHcDNA in five out of seven individuals and all three fishes with LRH3-GH2.8, indicating their transgenic nature.
Transgene integration was also studied by Southern hybridization of DNA isolated from blood of the transgenic fishes with
two different probes (histone 3 promoter and cDNA of L. rohita). Autotransgene integration was confirmed in all PCR positive transgenic individuals. The site of integration of the transgene
in the genome of the four transgenic fish could be determined by inverse PCR. Two individuals showed integration at the same
site whereas in the remaining two individuals the integration sites were different. 相似文献
48.
Abhijit?MajumdarEmail author Bijan?Sarkar Prabal?Kumar?Majumdar 《Fibers and Polymers》2004,5(4):297-302
In many engineering applications, the final decision is based on the evaluation of a number of alternatives in terms of a
number of criteria. This problem may become very intricate when the selection criteria are expressed in terms of different
units or the pertinent data are difficult to be quantified. The Analytic Hierarchy Process (AHP) is an effective way in dealing
with such kind of complicated problems. Cotton fiber is selected or graded, in the spinning industries, based on several quality
criteria. However, the existing selection or grading method based on Fiber quality Index (FqI) is rather crude and ambiguous.
This paper presents a novel approach of cotton fiber selection using the AHP methodology of Multi Criteria Decision Making. 相似文献
49.
Development of interspecific somatic hybrid cell lines in cultivated jute and their early characterization using jute chloroplast RFLP marker 总被引:1,自引:0,他引:1
A strong sexual incompatibility barrier that exists between the two cultivated jute species, Corchorus capsularis and Corchorus olitorius, limits the scope for improvement through genetic introgression. Protoplast fusion was carried out to generate interspecific hybrid cell lines. Cotyledonary cell protoplasts of C. capsularis and anthocyaninpigmented hypocotyl protoplasts of C. olitorius were used in the fusion experiments, which appeared to be visually useful in the early selection of the fused products. A chloroplast DNA (cpDNA) marker was developed in jute, which showed species‐specific hybridization patterns with EcoRI‐digested total genomic DNA of C. capsularis and C. olitorius. This cpDNA marker was used in the characterization of the somatic hybrid cell lines at their early stages of growth. Evidence for the presence of both types of cpDNA in the hybrid cell lines was obtained when the total genomic DNA of 4‐ to 7‐month‐old hybrid cell lines was challenged with the chloroplast DNA marker through Southern analysis. It was shown that the early segregation of the parental chloroplasts did not occur in jute, although this is common in other plant species. The hybrid nature of the fused cell lines could also be identified through peroxidase isozyme analysis. Isozyme banding patterns were complex and varied among the hybrid cell lines. 相似文献
50.
Abul Hashem M. N. Amin Majumdar Abdul Hamid M. M. Hossain 《Journal of Agronomy and Crop Science》1998,180(3):129-136
Drought stress effects on leaf gas exchange, cell membrane stability, seed yield and yield attributes of synthesized Brassica napus L. cv. Bangla kale and Bangla cabbage were compared. Drought stress treatments were imposed at early vegetative, late vegetative and flowering stages by withholding watering. Bangla cabbage produced greater pods/plant, larger seed size, greater total dry matter/plant, seeds/pot, and 17% greater yield than Bangla kale. The seed yield in plants stressed at early vegetative, late vegetative and flowering stages were 59, 74, 88% lower respectively, than watered plants. Drought stress reduced leaf photosynthesis by 67 to 97%. Bangla cabbage had 68% greater photosynthesis and 56% greater stomatal conductance than Bangla kale under stress at flowering stage. Leaf temperature was 1 to 2°C higher in stressed plants than watered plants. The cell membrane stability (CMS) increased up to 83% at flowering stage under stress compared to 21% under watered conditions. Although Bangla cabbage had high seed yield, yield attributes and photosynthesis under stressed conditions at flowering stage, its CMS values were lower than those of Bangla kale. 相似文献