Effect of parbendazole and piperazine adipate on the activity of some enzymes of Ascaridia galli and Heterakis gallinae     

R K Sharma  K Singh  K K Saxena 《Veterinary parasitology》1987,24(3-4):211-220

Adult Ascaridia galli and Heterakis gallinae obtained from the fowl (Gallus gallus) were treated in vitro with 10(-2) to 10(-5) M parbendazole and piperazine adipate for 10-60 min at 38 degrees C. Both the compounds at 10(-2) M caused mortality of A. galli and H. gallinae after a maximum of 30 min exposure. The effect of the drugs on the homogenates of the treated worm was investigated. Parbendazole (10(-2) M) inhibited malate oxidation by 68% in A. galli and 62% in H. gallinae. Piperazine adipate (10(-2) M) inhibited malate oxidation by 78% in both parasites. In A. galli oxaloacetate reduction was inhibited by 41 and 26% by 10(-2) M parbendazole and piperazine adipate, respectively; with H. gallinae this inhibition was found to be 39 and 55%, respectively. Aldolase activity in both the parasites was also inhibited by 10(-2) M parbendazole and piperazine adipate. Both compounds caused an inhibition of acid phosphomonoesterase activity, but the activities of lactate dehydrogenase and alkaline phosphomonoesterase were not affected significantly. Parbendazole (10(-2) M) had no significant effect on the cholinesterase activity of these parasites, but piperazine adipate (10(-2) M) caused an inhibition of 96% in A. galli and 93% in H. gallinae. The possible mode of action of the drugs is discussed.  相似文献   

Visceral candidiasis in a calf-case report     

S P Pachauri  V D Sharma  O P Saxena 《The Indian veterinary journal》1979,56(10):895-897

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Colonization of rice fields by Nilaparvata lugens (Stål) and its control using a trap crop     

R.C. Saxena 《Crop Protection》1982,1(2):191-198

Colonization of rice fields by the brown planthopper, Nilaparvata lugens (Stål) (Homoptera: Delphacidae), was monitored using yellow pan oil-water traps. In approximately 3 months, 35 788 immigrant macropters (1·0 ♂: 1·2 ♀) were caught in 512 traps installed over a 0·352 ha rice field. The trend of daily trap catches conformed closely to the number of macropters visually counted on rice hills. In sub-plots with a trap crop planted 20 days earlier than the main crop on a quarter of the total crop area, more macropters were recorded on trap-crop than on main-crop rows up to about 75 days after transplanting the main crop. In control sub-plots with one planting, more hoppers arrived on the middle than on the border rows. A blanket spray application of Perthane (0·75 kg a.i./ha) was made on the crop in control sub-plots, whereas only the trap crop was sprayed in trapped sub-plots. The combined yield in each treatment with trap crop was significantly higher than in the control treatment.  相似文献   

Bilateral corneal opacity in a dog--successful treatment with vit. A     

O P Saxena  G K Sinha  G A Shivnani 《The Indian veterinary journal》1967,44(5):434-437

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Identification of avian strains of Pasteurella multocida in India by conventional and PCR assays   总被引：1，自引：0，他引：1  

Shivachandra SB  Kumar AA  Gautam R  Singh VP  Saxena MK  Srivastava SK 《Veterinary journal (London, England : 1997)》2006,172(3):561-564

The prevalence of capsular and somatic serotypes were studied among 123 Pasteurella multocida strains isolated from chickens (n = 94), ducks (22), quails (4), turkeys (2) and geese (1) from different geographical regions of India. All strains exhibited similar cultural and morphological characteristics. Ninety-two of the isolates belonged to serotype A:1, the most prevalent serotype, with serotypes A:3, A:1,3, D:3 and F:3 having two isolates each. Only one isolate was positive for serotypes A:4 and D:1. Twenty isolates were untyped. A multiplex capsular PCR assay generated amplicons of sizes 460, 1044, 657 and 854 bp in 106 isolates identified as capsular serotype-A, 15 in serotype D and two in serotype F. Capsular types B and E were not detected in any of the avian isolates studied. The present findings suggest that a multiplex capsular PCR assay may be suitable for the rapid initial identification serotypes P. multocida during epidemiological studies of fowl cholera.  相似文献   

Genetic studies on primary antibody response to sheep erythrocytes in guinea fowl     

V. K. Saxena  H. Singh  S. K. Pal  S. Kumar 《British poultry science》1997,38(2):156-158

1. The primary antibody response to sheep erythrocytes was determined by haemagglutination test in guinea fowl. The effects of various genetic and non‐genetic factors on immune response to sheep RBCs in guinea fowl were also estimated.

2. The immune response to sheep RBCs was normally distributed in guinea fowl with mean titre at 1.534 ± 0.014.

3. In guinea fowl, effects on titre values of sire and variety (feather colour) were significant whereas sex and sex × variety interaction effects were non‐significant.

4. The estimate of heritability for immune response to sheep RBCs in guinea fowl was 0.35 ±0.17.  相似文献   

Some effects of confinement on laying pullets     

V. K. Guru  S. K. Saxena  S. P. Netke 《British poultry science》1974,15(3):333-337

Laying WLH pullets were housed individually in cages with floor areas of either 930 cm² (group 1) or 3700 cm² (group 2). Half the birds from each group were killed after 35 d confinement and the other half on the 70th d. Balance studies were conducted from 25th to 35th d and from 60th to 70th d.

The more severely confined birds had depressed plasma alkaline phosphatase activity but a raised plasma cholesterol level after 35 d. The alkaline phosphatase activity returned to normal after 70 d but the hypercholesterolaemia persisted. Acid phosphatase activity and plasma protein levels were unaffected by the treatments.

Pullets in the smaller cages laid fewer eggs but egg weight was unaffected.

Neither percentage metabolisable energy nor metabolisable nitrogen of the diet was affected by the severity of confinement, though less dietary nitrogen was retained by either group in the first half of the confinement period.

Severe confinement resulted in higher values of percentage metabolisable calcium and phosphorus by the 35th d but these decreased by the 70th d. In the less severely confined birds these values by the 70th d were higher than those at the 35th d.  相似文献   

Effect of Nitrogen and Sulphur Application on Yield and Fatty acid Composition of Mustard (Brassica juncea L.) Oil   总被引：1，自引：0，他引：1  

N. L. Joshi  P. C. Mali  Anurag Saxena 《Journal of Agronomy and Crop Science》1998,180(1):59-63

The effects of nitrogen and sulphur on the yield and fatty acid composition of mustard ( Brassica juncea L.) oil were studied in a field experiment. Significantly higher grain and oil yields were obtained with N and S application. Applications of nitrogen up to 60 kg ha⁻¹ and sulphur up to 40 kg ha⁻¹ favourably influenced the grain yield. Increasing levels of N decreased the oil content while application of sulphur improved the oil content. The contents of linoleic and linolenic acid were maximum (16.82 and 8.73%, respectively) with 60 kg N along with 40 kg S ha⁻¹. No use of fertilizers led to higher contents of undesirable fatty acids such as palmitic (hypercholesterimic) and erucic (do not have food value) acids.  相似文献   

Studies on fertility restoration of A4 cytoplasm in pigeonpea     

K. B. Saxena  R. V. Kumar  M. Bharathi 《Euphytica》2014,198(1):127-135

Fertility restoration of CMS-based hybrids is an integral part of breeding hybrids and the development of new hybrid parents with desirable agronomic and market preferred traits on regular intervals is essential for sustainability of such programs. This paper reports identification of 25 male-sterility maintainers and 179 fertility restorers of A₄ cytoplasm in pigeonpea (Cajanus cajan (L.) Millsp.). Multi-location evaluation of hybrids exhibited high stability for fertility restoration across diverse environments. The diversity study showed a large variation for important traits both at phenotypic as well as genetic levels. The potential use of this information in hybrid pigeonpea breeding has been discussed.  相似文献   

Genetic resources of rice in the Western Himalayan region of India: current status     

J. C. Rana  K. S. Negi  S. A. Wani  Sanjeev Saxena  K. Pradheep  Anjali Kak  S. K. Pareek  P. A. Sofi 《Genetic Resources and Crop Evolution》2009,56(7):963-973

The Western Himalayan region of India possesses rich genetic diversity of rice (Oryza sativa L.). Rice landraces having withstood the rigors of biotic and abiotic stresses, suit to the local conditions of farms and reflect socio-cultural preferences can still be found in crop fields located distantly in rural and tribal areas. This region is known for growing World fame basmati in the foot hills and many varieties of red rices grown at higher elevations, known for cold tolerance and medicinal properties. Genetic resources of rice were collected for about 8 years (1999–2006) and 1069 germplasm accessions including 154 named landraces were collected. Prominent landraces such as Begumi, Ramjwain, Thapachini, Naurang, Hansraj, Tilakchandan, Lalsati, Jhini, Mushakbudji, Jattoo, Barpasso, Qadirbeigh, Safedbrez, Shahie known for their special quality attributes were collected. The change in land use, cropping patterns and aggressive introduction of modern varieties in the region has resulted in the loss of a large number of landraces especially from irrigated lands. The present paper looks at the status of rice genetic resources, genetic variability, genetic erosion and future strategies to conserve rice germplasm on farm and to maximize its use in rice breeding.  相似文献