Colorimetric determination of tetracycline derivatives in pharmaceutical preparations     

U Saha 《Journal of the Association of Official Analytical Chemists》1989,72(2):242-244

A quick colorimetric method is reported for the determination of tetracycline derivatives such as oxytetracycline hydrochloride (OTCH), chlortetracycline hydrochloride (CTCH), methacycline hydrochloride (MCH), and doxycycline hydrochloride (DCH). The method involves complexation of the above derivatives with cupric chloride in alkaline medium. The yellowish green copper complexes of OTCH, CTCH, MCH, and DCH show maximum absorbance at 395, 410, 400, and 400 nm, respectively. The color intensity obeys Beer's law in the concentration range of 0-20 micrograms/mL.  相似文献   

Effects of inoculation of cyanobacteria on nitrogen status and nutrition of rice (Oryza sativa L.) in an Entisol amended with chemical and organic sources of nitrogen     

T. K. Ghosh  K. C. Saha 《Biology and Fertility of Soils》1996,24(1):123-128

A field experiment was conducted with wetland rice (Oryza sativa cv. IR-36) in a sandy clay loam soil (Entisol) to study the effect of inoculation with a soil-based mixed culture of four diazotrophic cyanobacteria,Aulosira fertilissima, Nostoc muscorum, N. commune andAnabaena spp., on the N-flux in inorganic NH₄ ⁺+NO₃ ^–+ NO₂ ^–), easily oxidizable, hydrolysable and non-hydrolysable forms of N in soil during vegetative growth periods of the crop. Effects on grain and straw yield and N uptake by the crop were estimated. The effects of applying urea N and N as organic sources, viz.Sesbania aculeata, Neem (Azardirachta indica) cake and FYM, each at the rate of 40 kg N ha^–1, to the soil were also evaluated. Inoculation significantly increased the release of inorganic N, evidenced by its increased concentrations either in soil or in soil solution. However, such increases rarely exceeded even 4% of total N gained in different froms in the soil system by inoculation during the vegetative growth stages of the rice plant, when the nutritional requirement of the plants is at a maximum. Most of the N₂ fixed by cyanobacteria remained in the soil as the hydrolysable form (about 85%) during this period. Inoculation caused an insignificant increase in grain (8%) and straw (11%) yield, which was, however, accompanied by a significant increase in N uptake by the grain (30%) and an increase in total uptake of 15.3 kg N ha 1. Such beneficial effects of inoculation varied in magnitude with the application of organic sources, with farmyard manure (FYM) being the most effective. Application of urea N, on the other hand, markedly reduced such an effect.  相似文献   

Changes in amount of organic and inorganic fractions of nitrogen in an Eutrochrept soil after long‐term cropping with different fertilizer and organic manure inputs     

Kotha Sammi Reddy  Muneshwar Singh  Awadesh Kumar Tripathi  Mahavir Singh  Manbendra Nath Saha 《植物养料与土壤学杂志》2003,166(2):232-238

The influence of 30 years of cropping with different fertilizer and farmyard manure (FYM) inputs on the contents and depth distribution of organic C, total N (N_t), soil mineralizable N, and organic and inorganic N fractions was investigated in an Eutrochrept. Continuous application of 100 %NPK(+S), 150 %NPK(+S), and 100 %NPK(+S)+FYM led to a marked increase in organic C, total N, hydrolyzable N (viz., amino acid N, hydrolyzable NH₄‐N, hexose amine N, and unidentified hydrolyzable N), and nonhydrolyzable N as compared to an adjacent fallow. The contents of the various organic N fractions were largest in surface soil and thereafter decreased with the depth. However, at 30 – 45 cm depth the content of organic C was not affected by the different treatments except 100 %NPK(+S)+FYM. On the other hand, continuous cropping without fertilization resulted in a depletion of total hydrolyzable N in control over fallow by 27.2 % (0–15 cm), 19.6 % (15–30 cm), and 4.7 % (30–45 cm). The incorporation of FYM with 100 %NPK(+S) resulted in greater contents of soil mineralizable N as compared to 100 %NPK(+S) (0–15, 15–30 cm). The proportion of hydrolyzable N (57–76 % of N_t) decreased and that of nonhydrolyzable N (22–40 % of N_t) increased with depth. The proportion of amino acid N (19–26 % of N_t), hexose amine N (2.1–3.5 % of N_t) and unidentified hydrolyzable N (17–27 % of N_t) decreased with depth. All organic soil N fractions including even nonhydrolyzable N in surface and subsurface soils were highly significantly correlated with soil mineralizable N derived from incubations under waterlogged and aerobic conditions. The best correlation to mineralizable N was found for amino acid N and the least significant correlation for nonhydrolyzable N.  相似文献   

A phosphatase associated with metastasis of colorectal cancer     

Saha S  Bardelli A  Buckhaults P  Velculescu VE  Rago C  St Croix B  Romans KE  Choti MA  Lengauer C  Kinzler KW  Vogelstein B 《Science (New York, N.Y.)》2001,294(5545):1343-1346

To gain insights into the molecular basis for metastasis, we compared the global gene expression profile of metastatic colorectal cancer with that of primary cancers, benign colorectal tumors, and normal colorectal epithelium. Among the genes identified, the PRL-3 protein tyrosine phosphatase gene was of particular interest. It was expressed at high levels in each of 18 cancer metastases studied but at lower levels in nonmetastatic tumors and normal colorectal epithelium. In 3 of 12 metastases examined, multiple copies of the PRL-3 gene were found within a small amplicon located at chromosome 8q24.3. These data suggest that the PRL-3 gene is important for colorectal cancer metastasis and provide a new therapeutic target for these intractable lesions.  相似文献   

Microwave-Enhanced Advanced Oxidation Treatment of Lipids and Food Wastes     

Asha Srinivasan  Moutoshi Saha  Kit Caufield  Otman Abida  Ping Huang Liao  Kwang Victor Lo 《Water, air, and soil pollution》2018,229(7):227

Fats, oils, and grease (FOG) and source separated organics (SSO) were treated with the microwave-enhanced advanced oxidation process (MW-AOP) at 90 and 110 °C, with varying amounts of hydrogen peroxide dosages. The treatment efficiency, in terms of soluble substrates and volatile fatty acids (VFA), increased with an increase in both temperature hydrogen peroxide dosages. Fatty acids and compounds with carbonyl group and/or hydroxyl group in both initial and treated FOG samples were identified by gas chromatography-mass spectrometry. MW-AOP treatment temperatures and hydrogen peroxide dosages dictated the formation of degradation products. The degradation followed peroxidation mechanism to produce lower molecular weight substrates such as short chain fatty acids which would be less inhibitory to microbes. After the MW-AOP treatment, both SSO and FOG comprised of more soluble and low molecular weight compounds. These compounds included VFA and nutrients that would be readily available for bacterial or plant uptake.  相似文献   

Soil Quality Changes Resulting from Long-Term Fertilizer Application Under Intensive Cropping System in Alluvial Soils     

A. K. Singh  S. P. Mazumdar  A. R. Saha  D. K. Kundu 《Communications in Soil Science and Plant Analysis》2017,48(13):1503-1510

Soil quality is one of the most important factors which reveal the soil–environment functionality for identifying whether soil quality is improving, remain constant, or declining. This paper evaluated change in soil quality after 40 years of chemical fertilizer use and continuous cultivation of multiple crops (jute–rice–wheat) on alluvial soils. The concepts of relative soil quality index (RSQI) and Cumulative Rating Index (CRI) were used in the evaluation. It was observed that soil of class III reduced to class II after 20 years and to class I after 40 years of fertilizer application with manure, nitrogen, phosphorus, and potassium (NPK_100+FYM). There was a strong correlation between SQI and CRI (r = 0.82**) which showed more promising effects on soil sustainability. Significant positive relationship between ?RSQI and crop yield of jute (r = 0.89**) and wheat (r = 0.90**) was found. However, rice yield declined up to 44% of its initial yield (r = 0.20).  相似文献   

Assessing suitability of different extractants for determining available boron in soil     

Aritra Saha  P. K. Mani  G. C. Hazra  Tarik Mitran 《Journal of plant nutrition》2017,40(19):2651-2661

In the present study, the extractability of available Boron (B) by different extractants was tested in relation to soil properties. Soil samples from different parts of Indo-Gangetic flood plains of West Bengal were collected and available B extracted with various extractant viz. hot calcium chloride (CaCl₂) (HCC), Potassium di-hydrogen phosphate (PDP), tartaric acid (TA) and MCC. Plant availability of B was assessed by growing wheat (Triticum aestivum L.cv.K-1006) in such soils through pot experiments. HCC showed the highest available B content as well as highest correlation coefficient value with all plant growth parameters and soil properties. The B extracting efficiency of the tested extractants was found in the order of HCC > PDP > TA > MCC. Results showed that dry matter yield, plant B concentration and uptake was increased significantly after application of Borax. Linear Correlations, Multiple regression analysis using Mallow's Cp statistics proved HCC to be the best extractant for estimating available B in experimental soils.  相似文献   

Genotypic comparisons of chromosomes 01, 04, and 18 from three tetraploid species of <Emphasis Type="Italic">Gossypium</Emphasis> in topcrosses with five elite cultivars of <Emphasis Type="Italic">G. hirsutum</Emphasis> L.     

Johnie N. Jenkins  Jack C. McCartyJr.  B. Todd Campbell  R. W. Hayes  Jixiang Wu  Sukumar Saha  David Stelly 《Euphytica》2017,213(5):107

Upland cotton, Gossypium hirsutum L. is the most widely planted cultivated cotton in the United States and the world. The other cultivated tetraploid species G. barbadense L. is planted on considerable less area; however, it produces extra long, strong, and fine fibers which spins into superior yarn. The wild cotton tetraploid species G. tomentosum Nuttall ex Seemann, native to the Hawaiian Archipelago also exhibits traits, such as drought tolerance, that would also be desirable to transfer to Upland cotton. Long-term breeding efforts using whole genome crosses between Upland and these species have not been successful in transferring very many desirable alleles into Upland cotton. Our chromosome substitution lines (CSL) have one chromosome or chromosome arm from an alien species backcrossed into the Upland cotton line,TM-1, via aneuploid technology. Five Upland cultivars were crossed with CS-B01, CS-T01, CS-B04, CS-T04, CS-B18 and CS-T18 and TM-1 the recurrent parent of the CSLs. This provided an opportunity to determine the effects of chromosomes 01, 04, and 18 from the three species in crosses with the five cultivars. Predicted genotypic mean effects of the parents, F₂, and F₃ generations for eight agronomic and fiber traits of importance were compared. The predicted hybrid mean effects for the three chromosomes from each species were different for several of the traits across cultivars. There was no single chromosome or species that was superior for all traits in crosses. Parental and hybrid lines often differed in the effect of a particular chromosome among the three species. The predicted genotypic mean effects for F₂ and F₃, with a few exceptions, generally agree with our previous results for additive and dominance genetic effects of these CSL.  相似文献   

Comparison of three extraction and cleanup methods for determining carbon-14-labeled residues from wheat plants grown in soil treated with dyfonate-ring-14C     

M Saha  A K Sumner  J G Saha 《Journal of the Association of Official Analytical Chemists》1973,56(1):45-48

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