Cellulase activity in rohu fingerlings     

Amlan Krishna Saha  Arun Kumar Ray 《Aquaculture International》1998,6(4):281-291

The activity of cellulase was determined in the intestine of rohu, Labeo rohita, fingerlings fed on separate dietary formulations incorporating Leucaena leaf meal and synthetic cellulose at 20% level in proportional replacement of the ingredients from the fish-meal-based reference diet. Three more replicate experimental diets were prepared by incorporating 1% tetra-cycline, a broad-spectrum antibiotic, into the former three dietary formulations so as to circumvent the action of microflora in digestive function. Cellulase activity was found to be highest in fish fed on the cellulose incorporated diet, followed by those maintained on the plant-protein-based and reference diets, respectively. A diet-dependent variation in cellulase activity was apparent. However, a sharp decline in the level of cellulase activity was observed in the fish fed diets containing tetracycline, which is supposed to have destroyed all the gut microflora. The microbial culture of intestinal and hepatopancreatic extracts also confirmed the absence of microflora in the fish fed tetracycline-compounded diets. The study indicates that cellulase activity in rohu is largely contributed by the intestinal microflora, while the reduced activity recorded in the fish reared on antibiotic-compounded diets may be due to the presence of some other source of cellulase secretion apart from cellulolytic microbial action. The information generated from the present investigation might contribute towards better feed formulation for carp at low cost, incorporating plant-based feed ingredients. © Rapid Science Ltd. 1998  相似文献   

Development of RAPD and ISSR derived SCAR markers linked to <Emphasis Type="Italic">Xca1Bo</Emphasis> gene conferring resistance to black rot disease in cauliflower (<Emphasis Type="Italic">Brassica oleracea</Emphasis> var. <Emphasis Type="Italic">botrytis</Emphasis> L.)     

Pritam Kalia  Partha Saha  Soham Ray 《Euphytica》2017,213(10):232

Black rot caused by Xanthomonas campestris pv. campestris (Xcc) (Pam.) is the most devastating disease of cauliflower (Brassica oleracea var. botrytis L.; 2n = 2x = 18), taking a heavy toll of the crop. In this study, a random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) derived sequence characterized amplified region (SCAR) markers linked to the black rot resistance locus Xca1bo were developed and evaluated as a screening tool for resistance. The RAPD marker OPO-04₈₃₃ and ISSR marker ISSR-11₆₃₅ were identified as closely linked at 1.6 cM distance to the black rot resistance locus Xca1bo. Both the markers OPO-04₈₃₃ and ISSR-11₆₃₅ were cloned, sequenced and converted into SCAR markers and validated in 17 cauliflower breeding lines having different genetic backgrounds. These SCAR markers (ScOPO-04₈₃₃ and ScPKPS-11₆₃₅) amplified common locus and showed 100% accuracy in differentiating resistant and susceptible plants of cauliflower breeding lines. The SCAR markers ScOPO-04₈₃₃ and ScPKPS-11₆₃₅ are the first genetic markers found to be linked to the black rot resistance locus Xca1bo in cauliflower. These markers will be very useful in black rot resistance marker assisted breeding.  相似文献   

Incidence of cystic mole in a cow     

A N Chakravarty  B N De  S K Saha  B K Basu 《The Indian veterinary journal》1965,42(9):701-702

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Organochlorine insecticide residues in soil from vegetable farms in Saskatchewan     

J G Saha  A K Sumner 《Pesticides monitoring journal》1971,5(1):28-31

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Use of RAPD-PCR for genetic analysis of Nicobari fowl of Andamans     

Ahlawat SP  Sunder J  Kundu A  Chatterjee RN  Rai RB  Kumar B  Senani S  Saha SK  Yadav SP 《British poultry science》2004,45(2):194-200

1. The present study was conducted to estimate genetic relatedness among Nicobari fowls (Brown, Black and White) and an exotic bird (White Leghorn) using random amplified polymorphic DNA (RAPD) polymorphism. 2. A total of 25 decamer primers were screened among all the breeds of which 24 primers amplified the genomic DNA, generating 2000 to 200 bp bands. Ten primers generated reproducible and distinct RAPD profiles and were used for further analysis. 3. A total of 94 bands were amplified and 30 polymorphic bands (32%) were produced. The number of polymorphic loci ranged from 1 to 5 with an average of 3.0. 4. Among the native breeds Brown Nicobari showed higher genetic similarity (0.85) than Black Nicobari (0.80) and White Nicobari fowl (0.82). 5. Brown Nicobari showed high genetic similarity with Black Nicobari (0.87 +/- 0.029); least similarity was between White Nicobari and White Leghorn (0.77 +/- 0.028). 6. The RAPD profile of all Nicobari fowls on amplification with the primers PBG5 and PBA12 showed specific bands of molecular size 1050 and 785 bp, respectively. 7. The native breeds showed the least genetic distance with each other while White Leghorn appeared to be most distant from the native breeds.  相似文献   

Haematic mummy and its incidence in a cross-bred cow     

B N De  S K Saha  A N Chakravarty  B K Basu 《The Indian veterinary journal》1965,42(9):699-701

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Fatty acid synthase as a potential therapeutic target in feline oral squamous cell carcinoma          下载免费PDF全文

J. Z. Walz  J. Saha  A. Arora  A. Khammanivong  M. G. O'Sullivan  E. B. Dickerson 《Veterinary and comparative oncology》2018,16(1):E99-E108

Oral squamous cell carcinoma (OSCC) is an aggressive and treatment‐resistant malignancy in both feline and human patients. Recent work has demonstrated aberrant expression of fatty acid synthase (FASN) and an increased capacity for lipogenesis in human OSCC and other cancers. In human OSCC, inhibition of FASN decreased cell viability and growth in vitro, and diminished tumour growth and metastasis in murine preclinical models. This study aimed to characterize FASN as a therapeutic target in feline OSCC. Immunohistochemistry revealed high FASN expression in primary feline OSCC tumours, and FASN expression was detected in OSCC cell lines (3 feline and 3 human) by immunoblotting and quantitative real‐time‐polymerase chain reaction (qRT‐PCR). Orlistat, a FASN inhibitor, substantially reduced cell viability in both feline and human OSCC lines, although feline cell lines consistently displayed higher sensitivity to the drug. FASN mRNA expression among cell lines mirrored sensitivity to orlistat, with feline cell lines expressing higher levels of FASN. Consistent with this observation, diminished sensitivity to orlistat treatment and decreased FASN mRNA expression were observed in feline OSCC cells following incubation under hypoxic conditions. Treatment with orlistat did not potentiate sensitivity to carboplatin in the cell lines investigated; instead, combinations of the 2 drugs resulted in additive to antagonistic effects. Our results suggest that FASN inhibition is a viable therapeutic target for feline OSCC. Furthermore, cats may serve as a spontaneous large animal model for human oral cancer, although differences in the regulation of lipogenesis between these 2 species require further investigation.  相似文献   

Milk whey as a serum supplement for the growth of FMD virus in BHK-21 cells     

S. N. Saha  A. K. Sen 《Veterinary research communications》1989,13(2):89-92

Two batches of experimental media containing 10% milk whey (sweet or sour) and 1% bovine serum supported the growth of BHK-21 cells in serial passages. The cell yield was greater than 2×10⁶/ml at 48 h. The cells were susceptible to inoculation with foot and mouth disease (FMD) virus type A and the virus titres obtained were comparable with those in Eagle's MEM-G with 10% serum. The use of milk whey could reduce the consumption of serum required for the growth of FMD virus in BHK-21 cells by up to 90%.  相似文献   

The Effect of K-Lactate Salt and Liquid Smoke on Bacterial Growth in a Model System     

Aparna Saha  Sveinung Birkeland 《Journal Of Aquatic Food Product Technology》2017,26(2):192-204

The effects of a commercial lactate salt formulation—containing potassium lactate (KL) and potassium acetate (KA)—and liquid smoke (LS) on the growth of selected lactic acid bacteria (LAB; Carnobacterium maltaromaticum, Carnobacterium inhibens, Lactococcus lactis, Lactobacillus curvatus, and Enterococcus faecalis), fish spoilage bacteria (Photobacterium phosphoreum, Pseudomonas putida, and Vibrio vulnificus), and Listeria innocua, were examined in a tryptic soy broth model system at 20ºC based on BioScreen data. The most pronounced inhibition effect on growth of bacteria was seen in the presence of 6% KL + 0.4% KA, used either combined with LS or alone. Only a minor inhibition effect on growth was found in the presence of LS alone. The only exception was Lactobacillus curvatus, which grew quite well in the presence of LS compared to control medium. The growth of Vibrio vulnificus was prevented in 6% KL + 0.4% KA, and significantly inhibited in the presence of 3% KL + 0.2% KA. When V. vulnificus was grown in NaCl, KA, and KL + KA, it was observed that KL + KA had a better inhibition effect than sodium salt within the same concentration range.  相似文献   

Interactive effect of biochar size and organic amendments on methane consumption in a tropical vertisol          下载免费PDF全文

B. Kollah  G. Dubey  P. Parasai  J. K. Saha  S. Gangil  S. R. Mohanty 《Soil Use and Management》2015,31(1):52-59

Biochar (BC) application to agricultural soil has been proposed as an effective countermeasure to mitigate climate change. A laboratory incubation experiment was carried out to gain insight into the effectiveness of BC on methane (CH₄) consumption in a tropical clayey vertisol. Except for the control treatment, BC of two different sizes (<0.25 or 0.25–2.00 mm) was mixed with vermicompost (VC), poultry manure (PM) or farmyard manure (FYM). BC and organic amendment were added to soil at 10% w/w and 80 kg N/ha, respectively. BC increased CH₄ consumption rate, k, in soil, irrespective of organic amendment type. The CH₄ consumption potential of soil was greater with the smaller size BC (<0.25 mm). Of the three organic amendments, VC exhibited the highest k (0.105) followed by FYM (0.093) and PM (0.072). BC (<0.25 mm) + PM was the most effective of the organic amendments in enhancing CH₄ consumption (k = 0.242). The lag phase varied between 7.3 day (control) and 1.0 day (soil + VC). Results revealed that there was a significant (P < 0.0001) effect of organic amendment and BC on CH₄ consumption, CO₂ production and microbial abundance. Cumulative CO₂ production (mg/g soil) varied between 2.15 (control) and 8.77 (soil + PM + BC < 0.25 mm). Pearson's correlation analysis showed significant correlation between CH₄ consumption and methanotrophs abundance (P < 0.001). The study shows that BC enhanced CH₄ consumption potential in agricultural land on a tropical vertisol, particularly using the smaller size (<0.25 mm), and could be an effective strategy to mitigate atmospheric CH₄.  相似文献