Removal of 17α-Ethinylestradiol by Biogenic Manganese Oxides Produced by the Pseudomonas putida strain MnB1     

Kim  Do-Gun  Jiang  Shaofeng  Jeong  Kwon  Ko  Seok-Oh 《Water, air, and soil pollution》2012,223(2):837-846

Synthetic and persistent endocrine disrupting chemicals (EDCs) such as 17α-ethinylestradiol (EE2) have been frequently detected in the effluent of wastewater treatment plants and induce hazards to humans and wildlife. In this study, biogenic Mn oxides were tested for the removal of EE2, and factors affecting the reaction were also investigated. The biogenic Mn oxides produced by Pseudomonas putida strain MnB1 were nano-sized and poorly crystallized particles. A concentration of 7.9 mg l⁻¹ biogenic Mn oxides showed 87% EE2 (1 mg l⁻¹) removal efficiency in 2 h, which confirms the excellent potential of biogenic Mn oxides for removal of estrogens. EE2 removal was enhanced at high Mn oxide doses and at low pH. Co-existing heavy metals significantly inhibit EE2 removal, due to their competition for the reactive sites of biogenic Mn oxides. Humic acid (HA) also obstructed EE2 removal, but the adverse effect was alleviated as HA concentration increased, possibly due to the formation of soluble complexes with the released Mn²⁺, of which adsorption onto Mn oxides reduces surface reactive sites.  相似文献   

Morpho-physiological response of common buckwheat (Fagopyrum esculentum) to flooding stress at different growth stages     

Choi  Ju-Young  Cho  Seong-Woo  Chun  Jae-Buhm  Kwon  Soo Jeong  Roy  Swapan Kumar  Sung  Jwa-Kyung  Woo  Sun-Hee  Sakagami  Jun-Ichi 《Journal of Crop Science and Biotechnology》2021,24(1):41-49

Journal of Crop Science and Biotechnology - Flooding is one of the major abiotic stresses which accounts for considerable damage to plant growth and development and induces multiple...  相似文献   

Anti-Human Rhinoviral Activity of Polybromocatechol Compounds Isolated from the Rhodophyta, Neorhodomela aculeata     

Soon-Hye Park  Jae-Hyoung Song  Taejung Kim  Woon-Seob Shin  Gab Man Park  Seokjoon Lee  Young-Joo Kim  Pilju Choi  Heejin Kim  Hui-Seong Kim  Dur-Han Kwon  Hwa Jung Choi  Jungyeob Ham 《Marine drugs》2012,10(10):2222-2233

An extract of the red alga, Neorhodomela aculeata, exhibited antiviral activity against human rhinoviruses. Bioassay-guided purification was performed to yield six compounds, which were subsequently identified as lanosol (1) and five polybromocatechols (2–6) by spectroscopic methods, including 1D and 2D NMR and mass spectrometric analyses. Structurally, all of these compounds, except compound 5, contain one or two 2,3-dibromo-4,5-dihydroxyphenyl moieties. In a biological activity assay, compound 1 was found to possess antiviral activity with a 50% inhibitory concentration (IC₅₀) of 2.50 μg/mL against HRV2. Compound 3 showed anti-HRV2 activity, with an IC₅₀ of 7.11 μg/mL, and anti-HRV3 activity, with an IC₅₀ of 4.69 μg/mL, without demonstrable cytotoxicity at a concentration of 20 μg/mL. Collectively, the results suggest that compounds 1 and 3 are candidates for novel therapeutics against two different groups of human rhinovirus.  相似文献   

Evaluation of waterlogging tolerance with the degree of foliar senescence at early vegetative stage of maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.)     

Seonghyu Shin  Seong-Guk Kim  Gun-Ho Jung  Chung-Guk Kim  Beom-Young Son  Jeong Tae Kim  Sang Gon Kim  Woonho Yang  Youngup Kwon  Kang-Bo Shim  Mi-Ok Woo 《Journal of Crop Science and Biotechnology》2016,19(5):393-399

Maize is highly susceptible to waterlogging, which is becoming one of worldwide abiotic threats in many agricultural areas. This study was evaluated to establish the screening method and to find tolerant maize genotypes. Six Korean maize inbred lines were subjected to waterlogging at V3 for 15 days using a big size pot with single maize plant (big pot method) and a box containing 31 maize plants at a time (box method). The degree of foliar senescence and the number of senescent leaves were better indicators for selecting waterlogging tolerant maize genotypes than SPAD value and plant height. The degree of foliar senescence revealed that KS124, KS140, and KS141 are tolerant, and KS85 is susceptible to waterlogging at the early growth stage. These responses of foliar senescence were in clear accordance with those of plant grain yield, which was supported by stress tolerance index for grain yield. The box method also showed the similar response of foliar senescence to the big pot method. Therefore, this box method based on foliar senescence may be simple and efficient for large-scale screening of maize germplasm against waterlogging stress. It was concluded that foliar senescence can be a good indicator for selecting tolerant maize genotypes against waterlogging at the early growth stage.  相似文献   

Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology     

Sen S  Ingale SL  Kim YW  Kim JS  Kim KH  Lohakare JD  Kim EK  Kim HS  Ryu MH  Kwon IK  Chae BJ 《Research in veterinary science》2012,93(1):264-268

Present study investigated the effect of dietary supplementation of Bacillus subtilis LS 1-2 on growth performance, apparent nutrient retention, caecal microbial population and intestinal morphology in broilers. Three hundred and twenty day-old Ross broiler chicks were randomly allotted to four treatments on the basis of BW in a randomized complete block design. Experimental diets were fed in two phases: starter (d 0-21) and finisher (d 22-35). Dietary treatments were basal diet supplemented with 0% (control), 0.15%, 0.30% and 0.45% B. subtilis LS 1-2. Supplementation of increasing levels of B. subtilis LS 1-2 showed linear improvement (P<0.05) in growth performance and apparent nutrient retention. At d 35, birds supplemented with increasing levels of B. subtilis LS 1-2 showed decrease in caecal Clostridium and Coliform count (linear, P<0.05). Moreover, supplementation of B. subtilis LS 1-2 increased (linear, P<0.05) villus height and villus height to crypt depth ratio in both duodenum and ileum. Results obtained in the present study indicate that B. subtilis LS 1-2 can be used as a growth promoter in broiler diets and can improve intestinal microbial balance and gut health of broilers.  相似文献   

Hematological adverse effects and pharmacokinetics of ribavirin in pigs following intramuscular administration          下载免费PDF全文

D.‐U. Lee  S. H. Je  S. J. Yoo  T. Kwon  J. Y. Shin  J. J. Byun  J.‐H. Park  K.‐w. Jeong  J.‐M. Ku  Y. S. Lyoo 《Journal of veterinary pharmacology and therapeutics》2017,40(5):561-568

Ribavirin (RBV) is a synthetic guanosine analog that is used as a drug against various viral diseases in humans. The in vitro antiviral effects of ribavirin against porcine viruses were demonstrated in several studies. The purposes of this study were to evaluate the adverse effects and pharmacokinetics of ribavirin following its intramuscular (IM) injection in pigs. Ribavirin was formulated as a double‐oil emulsion (RBV‐DOE) and gel (RBV‐Gel), which were injected into the pigs as single‐dose IM injections. After injection of RBV, all of the pigs were monitored. The collected serum and whole blood samples were analyzed by liquid chromatography–tandem mass spectrometry and complete blood count analysis, respectively. All of the ribavirin‐treated pigs showed significant decreases in body weight compared to the control groups. Severe clinical signs including dyspnea, anorexia, weakness, and depression were present in ribavirin‐treated pigs until 5 days postinjection (dpi). The ribavirin‐treated groups showed significant decrease in the number of red blood cells and hemoglobin concentration until 8 dpi. The mean half‐life of the RBV‐DOE and RBV‐Gel was 27.949 ± 2.783 h and 37.374 ± 3.502 h, respectively. The mean peak serum concentration (C_max) and area under the serum concentration–time curve from time zero to infinity (AUC_inf) of RBV‐DOE were 8340.000 ± 2562.577 ng/mL and 16 0095.430 ± 61 253.400 h·ng/mL, respectively. The C_max and AUC_inf of RBV‐Gel were 15 300.000 ± 3764.306 ng/mL and 207526.260 ± 63656.390 h·ng/mL, respectively. The results of this study provided the index of side effect and pharmacokinetics of ribavirin in pigs, which should be considered before clinical application.  相似文献   

Evaluation of Physiological Functionalities and Anti-inflammatory Activity on <Emphasis Type="Italic">in vitro</Emphasis> Cultured Adventitious Root of <Emphasis Type="Italic">Platycodon grandiflorum</Emphasis>     

Hee Ock Boo  Jeong Hun Park  Hag Hyun Kim  Soo Jeong Kwon  Sun Hee Woo 《Journal of Crop Science and Biotechnology》2018,21(2):183-191

This study was executed to evaluate the phenolic content, DPPH, ABTS radical scavenging rate, nitrite scavenging rate, and anti-inflammatory activity from in vitro cultured adventitious root extract of different Platycodon grandiflorum(PG) variety. Total polyphenol and flavonoid content showed the highest amount in adventitious root extract of tetraploid PG, and followed by green petal, double petal and diploid PG extract. The DPPH radical scavenging activity showed that the increase was proportional to the concentration, especially, the scavenging activity at a concentration of 10 mg mL^-1 was higher than 80%. The ABTS radical scavenging activity was not significantly different among the PG varieties at the concentration of 5 mg mL^-1 or more, and generally showed high scavenging activity. The nitrite scavenging activity was affected by pH, at pH of 1.2, the scavenging effect of all of the extracts tested observed higher than that of the other two pH ranges. The cytotoxicity of PG extract at various concentrations, the survival rate of RAW 264.7 cell was more than 90% at a concentration of 200 μg mL^-1 or less, and it was confirmed that the macrophage cell showed little toxicity. After PG extract pretreatment at 50, 100, and 200 μg mL^-1, LPS-treated experimental group significantly inhibited NO production in a dose-dependent manner. The dose-response trends followed quadratic regressions in all PG varieties. The PG extracts showed a considerable range of influence on cytokine secretion. The effect of adventitious root extracts of PG against inflammatory mediators TNF-α, IL-6 and IL-1β production showed the most significant anti-inflammatory activity.  相似文献   

Standardized total tract digestibility of phosphorus in various inorganic phosphates fed to growing pigs          下载免费PDF全文

Woong Bi Kwon  Beob Gyun Kim 《Animal Science Journal》2017,88(6):918-924

An experiment was conducted to determine the standardized total tract digestibility (STTD) of phosphorus (P) in five sources of inorganic phosphate fed to growing pigs, including dicalcium phosphate (DCP), monodicalcium phosphate (MDCP), monocalcium phosphate (MCP), tricalcium phosphate (TCP) and monosodium phosphate (MSP, reagent grade). Six barrows (42.4 ± 1.1 kg) individually housed in metabolism crates were allotted to a 6 × 6 Latin square design with six dietary treatments and six periods. Each experimental period consisted of a 4 day adaptation period and a 5 day collection period. The five experimental diets contained 0.24 to 0.34% of P from each inorganic phosphate as a sole source of P. A P‐free diet was also prepared to estimate the basal endogenous loss of P. The STTD of P in MSP (94.9%) was not different from the STTD of P in MCP (93.0%), but was greater (P < 0.05) than that in DCP, MDCP and TCP (87.0, 86.5 and 71.3%, respectively). In conclusion, digestibility of P in reagent‐grade MSP was greater than that in feed‐grade inorganic phosphates such as DCP, MDCP and TCP, and digestibility of P in DCP and MDCP was greater than that in the TCP.  相似文献   

Diagnostic utility of egg yolk for the detection of avian metapneumovirus antibodies in laying hens     

Choi KS  Lee EK  Jeon WJ  Park MJ  Yoo YN  Kwon JH 《Avian diseases》2010,54(4):1230-1236

Surveillance and diagnosis of avian metapneumovirus (AMPV) infection typically involve measurement of serum antibodies. In the current study, eggs instead of serum samples were used for the detection of AMPV antibodies in egg-laying chicken hens by enzyme-linked immunosorbent assay (ELISA). AMPV-free commercial layer hens were experimentally challenged with AMPV strain SC1509 through intravenous or oculonasal administration. Antibody levels were determined by ELISA. AMPV antibodies were detected in egg yolks from challenged hens by 7 days postinoculation (dpi), with the peak titer at 16 dpi. Antibody levels in eggs laid at 28 dpi correlated well (r = 0.93) with sera taken 28 dpi from the same hens. In a field trial of the yolk ELISA, six broiler breeder farms were surveyed, and all tested positive for AMPV antibodies in hen eggs, although positivity varied from farm to farm. Abnormal discolored eggs collected from outbreak farms had significantly higher titers of AMPV yolk antibodies than normal eggs from the same farm, unlike clinically healthy farms, where normal and abnormal eggs had similar antibody titers. These results indicate that diagnosis of AMPV infection by yolk ELISA to detect anti-AMPV antibodies may be a suitable alternative to serologic testing.  相似文献   

Different routes of inoculation impact infectivity and pathogenesis of H5N1 high pathogenicity avian influenza virus infection in chickens and domestic ducks     

Kwon YK  Swayne DE 《Avian diseases》2010,54(4):1260-1269

The H5N1 type A influenza viruses classified as Qinghai-like virus (clade 2.2) are a unique lineage of type A influenza viruses with the capacity to produce significant disease and mortality in gallinaceous and anseriform birds, including domestic and wild ducks. The objective of this study was to determine the susceptibility and pathogenesis of chickens and domestic ducks to A/Whooper Swan/Mongolia/224/05 (H5N1) high pathogenicity avian influenza (HPAI) virus when administered through respiratory or alimentary routes of exposure. The chickens and ducks were more susceptible to the H5N1 HPAI virus, as evidenced by low infectious and lethal viral doses, when exposed by intranasal as compared to alimentary routes of inoculation (intragastric or oral-fed infected chicken meat). In the alimentary exposure pathogenesis study, pathologic changes included hemorrhage, necrosis, and inflammation in association with virus detection. These changes were generally observed in most of the visceral organs of chickens, between 2 and 4 days postinoculation (DPI), and are similar to lesions and virus localization seen in birds in natural cases or in experimental studies using the intranasal route. Alimentary exposure to the virus caused systemic infection in the ducks, characterized by moderate lymphocytic encephalitis, necrotized hepatitis, and pancreatitis with a corresponding demonstration of virus within the lesions. In both chickens and ducks with alimentary exposure, lesions, virus, or both were first demonstrated in the upper alimentary tract on 1 DPI, suggesting that the alimentary tract was the initial site affected upon consumption of infected meat or on gavage of virus in liquid medium. However, as demonstrated in the infectivity study in chickens, alimentary infection required higher exposure doses to produce infection as compared to intranasal exposure in chickens. These data suggest that upper respiratory exposure to H5N1 HPAI virus in birds is more likely to result in virus infection and transmission than will consumption of infected meat, unless the latter contains high doses of virus, as found in cannibalized infected carcasses.  相似文献