Growth of the brittle star Ophiura sarsii sarsii in Funka Bay,Hokkaido, Japan     

Orino  Kazuki  Ishigane  Kosuke  Suzuki  Kota  Izumiura  Hiroki  Nakaya  Mitsuhiro  Takatsu  Tetsuya 《Fisheries Science》2019,85(4):705-716

Fisheries Science - The brittle star Ophiura sarsii with a disc diameter (DD) of less than 9 mm is an important prey item for flathead flounder Hippoglossoides dubius in Funka...  相似文献   

Interannual decrease in condition factor of the western sand lance Ammodytes japonicus in Japan in the last decade: Evidence for food‐limited decline of the catch     

Tetsuya Nishikawa  Yukinobu Nakamura  Shigeyoshi Okamoto  Hiroshi Ueda 《Fisheries Oceanography》2020,29(1):52-55

Annual catch of the western sand lance Ammodytes japonicus in the eastern Seto Inland Sea, Japan, has shown a decreasing trend since the 1990s. To examine whether food shortage was the main cause for the catch decrease, we investigated the condition factor of the age‐0 fish at the beginning of the estivation period (late July) in Harima‐Nada, eastern Seto Inland Sea, for 10 years from 2008 to 2017. The mean abundance of copepods as food for the age‐0 fish during the fish growth period (from February to June) around the estivation area was also determined. The condition factor showed a significant decrease, and values for 2011 and later years were mostly lower than the known minimum threshold (4.2) for maturation. In the recent 4 years from 2013 to 2016, the mean copepod abundance was much lower than values for the other years. The condition factor showed a significant positive correlation with the copepod abundance. These results indicate that decline of western sand lance catch after 1990 was caused mainly by food shortage.  相似文献   

Karnovsky’s fixative prevents artifacts appearing as vacuolation derived from tissue processing in kidneys treated with antisense oligonucleotide     

Hironobu Nishina  Tetsuya Sakairi  Akane Kashimura  Hiroko Sato  Mao Mizukawa  Yuhei Ozawa  Satomi Nishikawa 《Journal of toxicologic pathology》2021,34(4):367

Antisense oligonucleotide (ASO) therapies have been identified as a new treatment modality for intractable diseases. In kidneys treated with ASOs, vacuoles, in addition to basophilic granules, are often observed in the proximal tubules. Some reports have described that these vacuoles are likely to be a secondary phenomenon resulting from the extraction of ASOs during tissue processing. In this study, we compared renal morphology after fixation with Karnovsky’s fixative or 4% paraformaldehyde phosphate buffer (PFA) with that of 10% neutral-buffered formaldehyde solution (NBF). Female Sprague-Dawley rats, intravenously treated four times with 50 mg/kg locked nucleic acid containing antisense oligonucleotides (LNA-ASOs) for 1 or 2 weeks, were examined. Microscopically, vacuoles and basophilic granules in the proximal tubules were observed in the kidneys fixed with NBF. Basophilic granules are indicative of the accumulation of ASOs. Moreover, some of the vacuoles also contained faint basophilic granules, suggesting that the vacuoles were relevant to the accumulation of ASOs. Although moderate vacuolation was observed in the proximal tubules, the majority of the vacuolated epithelia were negative for kidney injury molecule-1 on immunohistochemical staining. Vacuoles in the proximal tubules were not observed in samples subjected to Karnovsky’s fixation, although basophilic granules were observed. In samples subjected to PFA fixation, vacuoles and basophilic granules were observed in the proximal tubules, similar to those in samples subjected to NBF fixation. Overall, our findings demonstrated the possibility of overestimation of vacuolation due to artifacts during tissue processing when using conventional NBF fixation. Karnovsky’s fixative is considered a useful alternative for distinguishing artificial vacuoles from true nephrotoxicity.  相似文献   

Elevated serum haptoglobin in pigs infected with porcine reproductive and respiratory syndrome virus.     

T Asai  M Mori  M Okada  K Uruno  S Yazawa  I Shibata 《Veterinary immunology and immunopathology》1999,70(1-2):143-148

We examined the two acute phase proteins, alpha (alpha)-1 acid glycoprotein (AGP) and haptoglobin (HP), in serum of pigs following experimental porcine reproductive and respiratory syndrome (PRRS) virus infection. Increased levels of serum HP, but not AGP, were observed from 7 to 21 days post-inoculation in the infected pigs. Furthermore, serum IL-6 increased in the infected pigs, but TNF-alpha did not. The increase of serum IL-6 in pigs following PRRS virus infection may induce production of HP. Also, in the field investigation, serum HP in pigs was dramatically increased after exposure to the PRRS virus.  相似文献   

Evaluation of rolling shear strength of plywood by flexural vibration method     

Heng Xu  Chiaki Tanaka  Tetsuya Nakao  Masahiro Yoshinobu  Hiroyuki Katayama 《Journal of Wood Science》1998,44(2):147-151

The rolling shear strength of plywood was evaluated using a flexural vibration test. Test specimens were lauan and Douglas fir three-ply plywoods made from thick veneers. The dynamic shear and Young's moduli were determined using the flexural vibration method, which involved in-plane and out-of-plane flexural vibration. The rolling shear strength was determined using the static destructive method, which is dependent on the direction of the lathe check in the core veneer. Before and after accelerated aging treatments were conducted, there were relations between out-of-plane dynamic properties (out-of-plane shear and Young's moduli) and its rolling shear strength. It was concluded that the rolling shear strength is related not only to the shear property of the core but the flexural stiffness of two faces when the deformation of out-of-plane plywood was not restrained.Part of this work was presented at the 47th Annual Meeting of The Japan Wood Research Society, Kochi, April 1997  相似文献   

Starch Characteristics of the Rice Mutant du2‐2 Taichung 65 Highly Affected by Environmental Temperatures During Seed Development     

Yasuhiro Suzuki  Yoshio Sano  Tetsuya Ishikawa  Tomoko Sasaki  Ushio Matsukura  Hiro‐Yuki Hirano 《Cereal Chemistry》2003,80(2):184-187

The effects of environmental temperature (21 vs. 28°C) during rice seed development on the starch characteristics (apparent amylose content, amylopectin chain length distribution, and gelatinization properties) of nonwaxy Taichung 65 (T65), waxy Taichung (T65wx), du2‐2 mutated low‐amylose strain Taichung (76‐3/T65), and Koshihikari were studied. Amylose contents increased with decreasing environmental temperatures. Analysis of the amylopectin chain length distribution showed that the relative amounts of long chains with degree of polymerization (DP) > 25 in all starches decreased if maturation occurred at 21°C. Gelatinization onset, peak, and conclusion temperatures and enthalpies decreased with decreasing environmental temperatures. Of all starches studied, the du2‐2 mutated low‐amylose Taichung (76‐3/T65) was most affected by maturation temperatures. These results indicate that the du2‐2 mutated low‐amylose Taichung (76‐3/T65) may be a useful strain in understanding biochemical and genetic starch biosynthesis response to slight changes in temperature.  相似文献   

Feeding,somatic condition and survival of sand lance <Emphasis Type="Italic">Ammodytes</Emphasis> sp. larvae in Mutsu Bay,Japan     

Nobuaki Nanjo  Tetsuya Takatsu  Kazuo Imura  Kingo Itoh  Yuuhei Takeya  Toyomi Takahashi 《Fisheries Science》2017,83(2):199-214

To clarify the recruitment process of sand lance Ammodytes sp., we investigated larval condition factor, relative gut fullness (%GF), prey abundance and oceanographic structure in Mutsu Bay, Japan, during 1999–2001. Ammodytes sp. larvae, which were collected by horizontal hauls of Motoda nets and a ring net at depths of 1, 10, 20, 30 and 40 m, were mainly distributed at 10–30 m. Larvae at the first feeding time until 12 mm in body length (BL) fed predominantly on copepod nauplii, whereas large larvae with BL of 12.1–14.0 mm fed on a mixture of copepod nauplii, copepodites and appendicularians from late February to April. A path analysis showed that difference in water density between 35- and 5-m depths negatively affected naupliar abundance at 10–30-m depth (standardised path coefficient β = ?0.71, p = 0.005 for 3.3–8.0-mm BL larvae and β = ?0.78, p < 0.001 for 8.1–12.0-mm BL larvae). Naupliar abundance positively affected the %GF of Ammodytes sp. larvae (β = 0.75, p < 0.001 for 3.3–8.0-mm BL larvae and β = 0.66, p < 0.001 for 8.1–12.0-mm BL larvae), whereas it was negatively affected by water temperature (β = ?0.45, p = 0.008 for 3.3–8.0-mm BL larvae and β = ?0.56, p = 0.002 for 8.1–12.0-mm BL larvae), and the temperature effect was weak compared with that of naupliar abundance. In turn, %GF positively affected larval somatic weight (β = 0.91, p < 0.001 for 6.0-mm BL larvae and β = 0.70, p = 0.005 for 10.0-mm BL larvae). The recruitment failure in 1999 was likely caused by a reduced condition factor, which resulted from low naupliar abundance. In contrast, the abundance of nauplii and Oithona similis copepodites was high in 2000 and 2001. It is possible that the higher recruitment success in 2001 was because of the higher water temperatures in Mutsu Bay, sustaining faster growth of the larvae than in 2000 under the high-prey abundance conditions.  相似文献   

Antigenic differences of the scuticociliate Miamiensis avidus from Japan     

J-Y Song  K Sasaki  T Okada  M Sakashita  H Kawakami  S Matsuoka  H-S Kang  K Nakayama  S-J Jung  M-J Oh  S-I Kitamura 《Journal of fish diseases》2009,32(12):1027-1034

In Japan and Korea, outbreaks of scuticociliatosis have frequently occurred in Japanese flounder, Paralichthys olivaceus . Morphological observations and small subunit rRNA gene sequences have shown that the causative agent of scuticociliatosis in the flounder is Miamiensis avidus (syn. Philasterides dicentrarchi ). In this study, we elucidated the antigenic differences between six Japanese M. avidus isolates as an initial step toward developing an effective vaccine against the disease. Four Japanese flounder isolates (IyoI, Nakajima, JF05To and Mie0301 isolates), one spotted knifejaw, Oplegnathus punctatus, isolate (SK05Kyo), and one ridged-eye flounder, Pleuronichthys cornutus , isolate (RF05To) were subjected to serological analysis. Antisera against IyoI, SK05Kyo, Nakajima and Mie0301 isolates were raised in rabbits and used for immobilization assays and Western blotting. Immobilization assays showed that the six isolates could be divided into three groups, tentatively designated serotype I for IyoI, JF05To, RF05To, SK05Kyo, serotype II for Nakajima and serotype III for Mie0301. Western blotting results supported these three serotypes, with marked similarities in the banding profiles of IyoI, JF05To, RF05To and SK05Kyo isolates, which were distinct from the Nakajima and Mie0301 isolates. Three isolates, IyoI, Nakajima and Mie0301 that were selected as representatives of each serotype, were highly pathogenic to Japanese flounder by experimental infection. Based on these findings, we propose that there are at least three M. avidus serotypes in Japan.  相似文献   

Exploration,conservation and evaluation of potato germplasm in Argentina     

K. A. Okada 《Potato Research》1976,19(3):263-269

Summary The activities of the Potato Germ Plasm Centre at Balcarce, Argentina, since its creation in 1970, are outlined. This Centre was developed by INTA (National Institute of Agricultural Technology) with West German bilateral assistance. Several collecting expeditions have been organized to north-western Argentina and as a result a valuable collection of germplasm is now available to potato breeders from both national and international institutions. Evaluation and utilization of this material are also being actively pursued, and the need for close international cooperation and coordination is stressed.  相似文献