Matrix metalloproteinase inhibitor RECK expression in canine tumors   总被引：5，自引：0，他引：5  

Takagi S  Kato Y  Asano K  Ohsaki T  Bosnakovski D  Hoshino Y  Okumura M  Kadosawa T  Fujinaga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(8):761-767

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Development of an efficient method for recovery of Puumala and Puumala-related viruses by inoculation of Mongolian gerbils     

Lokugamage N  Kariwa H  Lokugamage K  Hagiya T  Miyamoto H  Iwasa MA  Araki K  Yoshimatsu K  Arikawa J  Mizutani T  Takashima I 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(11):1189-1194

Puumala (PUU) virus and PUU-related viruses are difficult to isolate in cell culture. To determine whether animal inoculation would be a better alternative for virus recovery, the Sotkamo strain of PUU virus was inoculated into several animal species. Newborn Mongolian gerbils (MGs), mice, and rats were infected with the Sotkamo strain by intracerebral (ic), intraperitoneal (ip), and subcutaneous (sc) inoculation. Antibodies to PUU appeared in MGs at 30 days post-infection (dpi), and in mice and rats at 15 dpi. Interestingly, virus appeared at 7 dpi in lung and brain of MGs inoculated via ic and ip routes. Virus was detected in all tested tissues of MGs at 15 dpi, with a peak level of 1.36 x 10 (5) focus forming units (FFU)/g in brain tissue. The virus titer declined with the onset of the antibody response and became undetectable by 75 dpi, when the antibody titer reached the maximum level. The appearance of the virus in mice and rats was delayed as compared to MGs, and the virus titer was apparently lower, at approximately 4 to 8 x 10(3) FFU/g, at 15 dpi. In addition, lung homogenates of antibody-positive Clethrionomys (C.) rufocanus (captured in Tobetsu, Hokkaido, Japan) were inoculated into MGs by the ic route. PUU-related viral RNA was detected at 16 dpi in the brains of MG inoculated with the lung homogenate, and antibodies were detected at 45 dpi. These findings indicate that newborn MG inoculation is an efficient method to recover PUU and PUU-related viruses.  相似文献   

Effects of supplementary corn silage on the feed intake and milk production of time-restricted grazing dairy cows     

Tomohiro MITANI  Makoto TAKAHASHI  Koichiro UEDA  Hiroki NAKATSUJI  Seiji KONDO  Masahiko OKUBO 《Animal Science Journal》2005,76(4):331-337

The effects of supplementary corn silage (CS) of either 2 or 4 kg of dry matter (DM; S + 2 and S + 4, respectively) above the energy requirement for milk production and maintenance for grazing dairy cows (S) were determined. Time‐restricted grazing was used to compare the feed intake, milk production, and nitrogen and energy use of lactating cows. The experiment was carried out on two different pastures using a 3 × 3 Latin square design for each pasture. Cows were grazed for 5 h on a rotational grazing system and were fed concentrate (1 kg per 5 kg of milk yield). Herbage intake was measured using a weighing technique. To calculate the energy and nitrogen use, whole feces and urine were collected. There was no statistical effect of the pastures. Herbage intake decreased by the addition of CS (P = 0.02). The reduction of herbage DM intake per unit consumption of supplementary CS towards the S group were 0.80 and 0.45 kg for the S + 2 and S + 4 groups, respectively. The total DM intake for the S + 4 group was higher than that for the S and S + 2 groups (P = 0.02). Milk yield did not differ among treatments, even though the total DM intake for the S + 4 group was higher than that of the S and S + 2 groups. Nitrogen and energy use did not differ with the addition of CS.  相似文献   

Light quality of continuous illuminating at night to induce floral initiation of Fragaria chiloensis L. CHI-24-1     

Tomohiro Yanagi  Takanori Yachi  Nobuaki Okuda  Kensyo Okamoto 《Scientia Horticulturae》2006

A wild strawberry strain, Fragaria chiloensis CHI-24-1, produced inflorescences from both parent and asexually propagated daughter plants linked with runners when grown at 23 °C/20 °C (day/night) under a 24 h day-length (DL) of daylight plus nightly lighting by an incandescent lamp, but not under 8 or 16 h DLs. In the present study, the effect of light quality for continuous illuminating at night on floral initiation of CHI-24-1 plants grown under a 24 h DL was examined. The CHI-24-1 plants were grown under a 24 h DL consisting of natural daylight and continuous lighting at night by an incandescent, a blue fluorescent, a red fluorescent or a far-red fluorescent lamp for 40 days in summer and autumn. Also, the CHI-24-1 plants were grown for 40 days in a growth chamber at 25 °C/20 °C (day/night) with natural daylight and continuous lighting at night by red- and four types of far-red light-emitting-diodes (LEDs with peak wavelengths of 660, 700, 735, 780 and 830 nm). In both experiments, floral initiation of the parent and daughter plants was observed under a stereomicroscope. Although more than 50% of the parent and daughter plants initiated flower buds under the incandescent and far-red fluorescent lamps, about 15% and 0% of those initiated flower buds under blue and red fluorescent lamps, respectively. Floral initiation of the parent and daughter plants occurred under the far-red LED light source whose peak wavelength was 735 nm, but not under the red or the other far-red LEDs. From these results, it can be concluded that the effective light wavelength range of nightly continuous illuminating for floral induction in the CHI-24-1 plants is 735 nm in the far-red light region. Hence, the induction of floral initiation by nightly continuous far-red light (735 nm) appeared to be a response mediated by phytochrome.  相似文献   

The increment of anti-ORP150 autoantibody in initial stages of atheroma in high-fat diet fed mice     

Kobayashi T  Yura T  Yanagi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(2):177-180

Expression of 150 kda oxygen-regulated protein, ORP150, was examined in the atheromatous lesions on aortic valves in high-fat diet fed mice. Immunohistochemical staining revealed that ORP150 was expressed on the surface of plaque and was co-localized with phagocytes bearing Mac-3, a mouse macrophage differentiation antigen. These findings suggest that ORP150 is involved in the development of the atheromatous plaque. Titer of autoantibody against ORP150 was gradually elevated in parallel with the length of period of high-fat diet feeding. These results suggest that the deposition of immunocomplex toward ORP150 antigen is involved in atheromatous plaque progression.  相似文献   

Effects of chronic oral amiodarone on left ventricular function,ECGs, serum chemistries,and exercise tolerance in healthy dogs     

Bicer S  Nakayama T  Hamlin RL 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(3):247-254

Amiodarone, a class III antiarrhythmic with beta-adrenergic blocking and antimuscarinic properties, has a wide spectrum of clinical use in humans. This study was conducted to establish the effects of a 25 mg/kg q12h loading dose and a 30 mg/kg q24h maintenance dose of amiodarone, each given PO for 3.5 weeks, on systemic arterial pressure, echocardiographic (ECHO) indices of left ventricular function, ECGs, exercise tolerance, and serum biochemistries in adult, clinically normal dogs. Means were calculated and were compared by analysis of variance with repeated measures. When a significant F statistic was identified, specific means were compared by Bonferroni's post hoc test. Body weight and heart rate (HR) decreased, and PQ, QT, and corrected QT (QTc) increased significantly (P < .05) for the weeks that the dogs received the loading dose, but all parameters returned to values the same as those in the pretest for the weeks when dogs received the maintenance dose. Serum activity of hepatic enzyme activities and cholesterol concentrations increased, and serum concentrations of thyroid hormones (triiodothyronine [T3] and thyroxine [T4]), phosphorous, and total carbon dioxide decreased. The changes in PQ, QT, and QTc are similar to those obtained previously, but the detailed ECG and ECHO observations have not been reported. A dose of 25 mg/kg q12h, but not 30 mg/kg q24h, is an appetite suppressant, and the lower dose produces neither ECG nor ECHO changes of clinical or toxicological significance in normal dogs.  相似文献   

Functional disorder of the retina in manganese-deficient Japanese quail revealed by electroretinography using a contact lens electrode with built-in light source     

Endo K  Itoh N  Maehara S  Shinozaki A  Imagawa T  Uehara M  Mizuno N  Sasaki S  Hiraga T  Teraoka H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(2):139-144

Manganese deficiency results in neurological and skeletal defects, together with ultrastructural disarrangement of the retina in rats. Wild birds show a range of Mn concentrations in their tissues, including the liver, raising the possibility of Mn-related disorders in the wild. Electroretinography (ERG) provides a useful noninvasive approach to evaluate visual function. This method is especially useful in birds, as objective analysis of them is very difficult, while they have well-developed vision. In this study, we carried out a convenient and reliable ERG recording using a contact lens electrode with a built-in light source (LED electrode) of Japanese quail (Coturnix coturnix japonica) fed a Mn-deficient diet. After 10 min light adaptation, single-flash and flicker cone responses were reproducibly recorded to cause an intensity-dependent increase in amplitude of both a-wave and b-wave in single-flash ERG. Mn-deficient feeding markedly decreased the Mn concentration in the liver by almost half in 3 to 6 weeks, followed by body weight loss in 13 to 15 weeks. Implicit time of a-wave and b-wave cone response by single-flash stimulation was significantly delayed in quail with a Mn depletion from 3 to 6 weeks. Every cone response of the Mn-deprived quail had a tendency to decrease amplitude. The ultrastructure of cone photoreceptor cells was disorganized by Mn deficiency, including changes in outer segment discs of photoreceptor cells. These results suggest the essential role of Mn in the integrity of the retinal function of birds.  相似文献   

Availability of oral swab sample for the detection of bovine viral diarrhea virus (BVDV) gene from the cattle persistently infected with BVDV   总被引：2，自引：0，他引：2  

Tajima M  Ohsaki T  Okazawa M  Yasutomi I 《The Japanese journal of veterinary research》2008,56(1):3-8

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Sodium channel mutations (T929I and F1534S) found in pyrethroid-resistant strains of the cigarette beetle,Lasioderma serricorne (Coleoptera: Anobiidae)     

Naoto Fukazawa  Ryota Takahashi  Hinako Matsuda  Yuya Mikawa  Toshiyuki Suzuki  Tomohiro Suzuki  Shoji Sonoda 《Journal of Pesticide Science》2021,46(4):360

RNA-seq data analysis of cigarette beetle (Lasioderma serricorne) strains having different sensitivities to pyrethroids identified sodium channel mutations in strains showing pyrethroid resistance: the T929I and F1534S mutations. These results suggest that reduced sensitivity of the sodium channel confers the pyrethroid resistance of L. serricorne. Results also showed that the F1534S mutation mostly occurred concurrently with the T929I mutation. The functional relation between both mutations for pyrethroid resistance is discussed.  相似文献   

Parentage analysis of tea cultivars in Japan based on simple sequence repeat markers     

Nakao Kubo  Tomohiro Matsuda  Chisaki Yanagida  Yuna Hotta  Yutaka Mimura  Maho Kanda 《Breeding Science》2021,71(5):594

Tea cultivars have been bred by individual selection of landraces and by crossbreeding, but the validation of the parentage is limited. In this study, we performed parentage analysis of 79 tea cultivars in Japan based on SSR markers to confirm or identify the parent-offspring relationships among them. The effectiveness of nine SSR markers for parentage analysis was validated by comparing them to the existing cleaved amplified polymorphic sequence markers. The former markers were detectable more alleles than the latter. Simulation of parentage analysis of the tea cultivars predicted biparental origins for 12 cultivars (‘Houshun’, ‘Mie ryokuhou no. 1’, ‘Surugawase’, ‘Tenmyo’, ‘Yamanoibuki’, ‘Harumidori’, ‘Koushun’, ‘Minekaori’, ‘Okumusashi’, ‘Saemidori’, ‘Sofu’, and ‘Toyoka’), in the first five of which candidate parents of yet-to-be-defined pedigree were newly identified. Comparisons of a total of 41 SSR genotypes confirmed the newly-identified parentages of ‘Asahi’ for ‘Tenmyo’, ‘Rokurou’ for ‘Houshun’, ‘Surugawase’, and ‘Yamanoibuki’, and ‘Yamatomidori’ for ‘Mie ryokuhou no. 1’. The maternity of seven cultivars out of the 12 was also confirmed with chloroplast DNA sequences. Uniparental origins were confirmed for 25 cultivars. This parentage analysis has improved our knowledge of tea pedigrees and will aid in the development of new cultivars.  相似文献