Pathogenicity of emerging Japanese type 1 porcine reproductive and respiratory syndrome virus in experimentally infected pigs     

Hiroshi ISEKI  Michihiro TAKAGI  Kenji KAWASHIMA  Tomoyuki SHIBAHARA  Yoshiko KURODA  Hiroshi TSUNEMITSU  Makoto YAMAKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1663-1666

To clarify the pathogenicity of Japanese type 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolate in experimentally infected pigs, we evaluated clinical signs and monitored viremia for 21 days post-inoculation (dpi). Lungs were mottled, tanned and reddish in appearance; had lesions predominantly in the cranial, middle and accessory lobes; and failed to collapse at 10 dpi. Although microscopic lesions of lungs were reproduced using the Japanese emerging type 1 PRRSV isolate under experimental conditions, no significant differences were noted between the challenge and control groups regarding mean rectal temperature and daily weight gain. These results provide useful insights into the limited pathogenicity of single infection with the Japanese type 1 PRRSV isolate in piglets, which differ from findings in reported field cases.  相似文献   

Effects of utilization of local food by‐products as total mixed ration silage materials on fermentation quality and intake,digestibility, rumen condition and nitrogen availability in sheep          下载免费PDF全文

Srita Yani  Kyohei Ishida  Shuzo Goda  Shigeyoshi Azumai  Tomoyuki Murakami  Masayuki Kitagawa  Kanji Okano  Kazato Oishi  Hiroyuki Hirooka  Hajime Kumagai 《Animal Science Journal》2015,86(2):174-180

Four wethers were used in a 4 × 4 Latin square design experiment to evaluate in vivo digestibility of total mixed ration (TMR) silage with food by‐products for dairy cows, and the ruminal condition and nitrogen (N) balance were examined. Five by‐products (i.e. potato waste, noodle waste, soybean curd residue, soy sauce cake and green tea waste) were obtained. Four types of TMR silage were used: control (C) containing roughage and commercial concentrate, T1:20% and T1:40% containing the five by‐products replacing 20% and 40% of the commercial concentrate on a dry matter (DM) basis, respectively, and T2:40% containing three by‐products (potato waste, noodle waste and soybean curd residue) replacing 40% of the commercial concentrate on a DM basis. The ingredients were mixed and preserved in oil drum silos for 4 months. The TMR silages showed 4.02–4.44% and 1.75–2.19% for pH and lactic acid contents, respectively. The digestibility of DM and neutral detergent fiber, and total digestible nutrient content were higher (P < 0.05) for T2:40% feeding than for C feeding. Urinary nitrogen excretion tended to be lower (P = 0.07) for T2:40% than for C. The results suggested 40% replacing of commercial concentrate by using the three food by‐products can be most suitable for TMR silage.  相似文献   

Reddish coloration of Chinese quince (Pseudocydonia sinensis) procyanidins during heat treatment and effect on antioxidant and antiinfluenza viral activities     

Hamauzu Y  Kume C  Yasui H  Fujita T 《Journal of agricultural and food chemistry》2007,55(4):1221-1226

To investigate the effects of processing Chinese quince fruit on the denaturation of phenolics and their food functions, fruit phenolic extracts were heated together with organic acid for up to 12 h. Chinese quince phenolic (mostly procyanidins) solution subjected to heat treatment changed from almost colorless, pale yellow, to a reddish color. Before heat treatment, the absorption spectra of polymeric procyanidins were observed only around 280 nm; after heat treatment, absorption occurred between 400 and 600 nm, which is related to the reddish color appearance. Thioacidolysis of denatured reddish phenolics showed that (-)-epicatechin subunits decreased during heat treatment and, in contrast, cyanidin increased. In addition, novel substances that could not be degraded by thioacidolysis were formed. Meanwhile, antioxidant activities, assessed by linoleic acid peroxidation, 1,1-diphenyl-2-picrylhydrazyl (DPPH), Folin-Ciocalteu, and FRAP methods, increased during heat treatment. The antiinfluenza viral activity of denatured reddish phenolics was inferior to that of intact fruit phenolics; however, they retained moderate activity. These results indicate that red coloration of fruit products of Chinese quince was mainly due to the spectral (i.e. structural) changes of procyanidins accompanied with formation of cyanidin. Increasing the length of heat treatment increased the antioxidant capacity of phenolics, and the resultant reddish phenolics retained moderate antiinfluenza viral activity.  相似文献   

Structural Changes of Goat Parotid Salivary Gland: Pre‐ and Post‐Weaning Periods     

Y. H. A. Elewa  O. Ichii  S. Otsuka  Y. Hashimoto  Y. Kon 《Anatomia, histologia, embryologia》2014,43(4):265-272

Previously, the structure of the adult goat parotid salivary glands (PGs) was studied. However, little information was elucidated of the juvenile ones. This study aimed to clarify the correlations between the structure of goats' PGs and the nature of food intake among milk‐suckling kids (MSKs) and diet‐fed goats (DFGs). The secretory endpieces of the goats' PGs are of the pure serous type. The serous cells in MSKs showed apical accumulation of numerous secretory granules (SGs) of smaller size and of more intense positive periodic acid‐Schiff reaction. Ultrastructurally, most of the SGs in the DFGs contained peripherally located inclusions that showed dense reaction products for acid phosphatase. In MSKs, the PGs showed less‐developed basal infoldings, sparseness of the inter‐cellular inter‐digitations, fewer inter‐cellular canaliculi and microvilli and also less‐developed myoepithelial cells with fewer and shorter cytoplasmic processes. In conclusion, the less‐developed membrane specializations and myoepithelial cells, as well as the accumulated SGs in the PGs of MSKs, suggest that it secretes less saliva with a little secretory activity than that of DFGs, which may be correlated with the reduced masticatory activity.  相似文献   

Characterization of the quantitatively major collagen in the mantle of oyster <Emphasis Type="Italic">Crassostrea gigas</Emphasis>     

Shoshi?MizutaEmail author  Tomoyuki?Miyagi  Reiji?Yoshinaka 《Fisheries Science》2005,71(1):229-235

ABSTRACT:   A quantitatively major collagen was isolated from the pepsin-solubilized collagen preparation of the mantle by differential salt precipitation and phosphocellulose column chromatography, and its constituent α components (α1 and α2) were purified by phosphocellulose column chromatography. The subunits were demonstrated to be genetically distinct from each other by peptide mapping and amino acid analysis. The amino acid composition calculated from those of the α1 and α2 components in 2 : 1 ratio coincided well with that of the major collagen from the mantle. These results suggest that the major collagen in the mantle of the oyster may have a heterotrimer structure (α1)₂α2.  相似文献   

Preparation of antiserum against carp (Cyprinus carpio L.) immunoglobulin and its application for immunohistochemistry     

T Imagawa  Y Hashimoto  B Syuto  Y Kon  M Sugimura 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(6):1329-1332

  相似文献   

Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution     

Yasuyuki Onodera  Takumi Arakawa  Rika Yui-Kurino  Masayuki P. Yamamoto  Kazuyoshi Kitazaki  Shigehiko Ebe  Muneyuki Matsunaga  Kazunori Taguchi  Yosuke Kuroda  Shiko Yamashita  Tomoyuki Sakai  Toshiro Kinoshita  Tetsuo Mikami  Tomohiko Kubo 《Euphytica》2015,206(2):365-379

  相似文献   

Studies on absorption and hydrolysis of ethyl alpha-D-glucoside in rat intestine     

Mishima T  Tanaka K  Tsuge H  Sugita J  Nakahara M  Hayakawa T 《Journal of agricultural and food chemistry》2005,53(18):7257-7261

Ethyl alpha-D-glucoside (alpha-EG) is normally contained in Sake, which has been taken by Japanese people since ancient times. In this study, the intestinal absorption of alpha-EG was investigated using rat everted intestinal sac. Furthermore, the alpha-EG hydrolytic activity in rat intestine was compared with disaccharides hydrolytic activities, and the effects of alpha-EG on disaccharides hydrolysis were examined using crude enzyme preparation from rat intestinal acetone powder. Glucose liberated from alpha-EG was detected in a serosal solution of everted rat intestinal sac, but it was only less than 4% of absorbed intact alpha-EG. alpha-EG absorption into small intestinal tissue was reduced by elimination of sodium ion from the mucosal solution or under the presence of phlorizin. The hydrolytic activity for alpha-EG was detected in crude enzyme preparation from rat intestinal acetone powder, but it showed a low value as compared to those for disaccharides. alpha-EG showed mixed type inhibition for maltose and sucrose hydrolysis, but inhibitory concentrations of alpha-EG required for 50% inhibition for the maltose and sucrose hydrolysis were higher than those of arabinose and acarbose. In conclusion, a small amount of alpha-EG was hydrolyzed and most of it was absorbed via SGLT1 as an intact form in the rat small intestine, and the inhibitory effect of alpha-EG on disaccharides hydrolysis was weak.  相似文献   

Purification and characterization of a corrinoid compound from Chlorella tablets as an algal health food     

Kittaka-Katsura H  Fujita T  Watanabe F  Nakano Y 《Journal of agricultural and food chemistry》2002,50(17):4994-4997

Vitamin B(12) content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9-211.6 microg/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3-285.7 microg/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-Vis spectroscopy.  相似文献   

不同水分管理对水稻生长与氮素利用的影响   总被引：13，自引：1，他引：13  

沈阿林  昆虫男 《植物营养与肥料学报》1997,3(2):111-116

应用土柱模拟法和15N示踪技术研究了长期淹水和严重渗漏条件下水稻的生长和氮肥的吸收与转化。结果表明，两种水分条件下，水稻根系生长量和地上部干物质积累量明显不同。水稻在淹水条件下因根系生长受阻，氮积累在拔节后明显变缓；而渗漏条件下水稻对氮的吸收在孕穗期仍保持较高水平。前者对肥料氮的利用率较后者显著地低，且土壤矿化氮在所吸收的氮中的贡献率也相对较高。这与水稻在两种水分管理条件下的生长状况和氮在两种情形下的转化特点密切相关。本试验条件下，水稻于拔节后对土壤矿化氮的吸收数量明显增加，似有脱肥现象，因此，应提倡追施孕穗肥。  相似文献