Identification of a novel cytotoxic protein, Cry45Aa, from Bacillus thuringiensis A1470 and its selective cytotoxic activity against various mammalian cell lines     

Okumura S  Saitoh H  Ishikawa T  Wasano N  Yamashita S  Kusumoto K  Akao T  Mizuki E  Ohba M  Inouye K 《Journal of agricultural and food chemistry》2005,53(16):6313-6318

Parasporal inclusion proteins produced by Bacillus thuringiensis strain A1470 exhibit strong cytotoxicity against human leukemic T cells when activated by protease treatment. One of the cytotoxic proteins was separated by anion exchange chromatography and gel filtration chromatography and designated Cry45Aa. Its gene was then expressed in recombinant Escherichia coli, in which the Cry45Aa precursor was accumulated in an inclusion body. It was solubilized in sodium carbonate buffer and processed with proteinase K, and cytotoxic activities of the protein against various mammalian cell lines were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay. The protein exhibited high cytotoxic activity against CACO-2, Sawano, MOLT-4, TCS, and HL60 cells and moderate activity against U-937 DE-4, PC12, and HepG2 cells. On the other hand, the EC50 values against Jurkat, K562, HeLa, A549, Vero, COS-7, NIH3T3, CHO, and four normal tissue cells (human primary hepatocyte cells, UtSMC, MRC-5, and normal T cells) were >2 microg/mL.  相似文献   

CTLA-4 control over Foxp3+ regulatory T cell function   总被引：1，自引：0，他引：1  

Wing K  Onishi Y  Prieto-Martin P  Yamaguchi T  Miyara M  Fehervari Z  Nomura T  Sakaguchi S 《Science (New York, N.Y.)》2008,322(5899):271-275

Naturally occurring Foxp3+CD4+ regulatory T cells (Tregs) are essential for maintaining immunological self-tolerance and immune homeostasis. Here, we show that a specific deficiency of cytotoxic T lymphocyte antigen 4 (CTLA-4) in Tregs results in spontaneous development of systemic lymphoproliferation, fatal T cell-mediated autoimmune disease, and hyperproduction of immunoglobulin E in mice, and it also produces potent tumor immunity. Treg-specific CTLA-4 deficiency impairs in vivo and in vitro suppressive function of Tregs-in particular, Treg-mediated down-regulation of CD80 and CD86 expression on dendritic cells. Thus, natural Tregs may critically require CTLA-4 to suppress immune responses by affecting the potency of antigen-presenting cells to activate other T cells.  相似文献   

Production of trout offspring from triploid salmon parents     

Okutsu T  Shikina S  Kanno M  Takeuchi Y  Yoshizaki G 《Science (New York, N.Y.)》2007,317(5844):1517

Many salmonids have become at risk of extinction. For teleosts whose eggs cannot be cryopreserved, developing techniques other than egg cryopreservation to save genetic resources is imperative. In this study, spermatogonia from rainbow trout were intraperitoneally transplanted into newly hatched sterile triploid masu salmon. Transplanted trout spermatogonia underwent spermatogenesis and oogenesis in male and female recipients, respectively. At 2 years after transplantation, triploid salmon recipients only produced trout sperm and eggs. With use of these salmon as parents, we successfully produced only donor-derived trout offspring. Thus, by transplanting cryopreserved spermatogonia into sterile xenogeneic recipients, we can generate individuals of a threatened species.  相似文献   

Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse     

Yamashita T  Hige T  Takahashi T 《Science (New York, N.Y.)》2005,307(5706):124-127

Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as "kiss-and-run" was unrelated to transmitter release. The release-related capacitance change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine 5'-triphosphate (GTP) analog GTPgS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.  相似文献   

Impressions and purchasing intentions of Japanese consumers regarding pork produced by ‘Ecofeed,’ a trademark of food‐waste or food co‐product animal feed certified by the Japanese government     

Keisuke SASAKI  Hideo AIZAKI  Michiyo MOTOYAMA  Hideyuki OHMORI  Tomoyuki KAWASHIMA 《Animal Science Journal》2011,82(1):175-180

Impressions and purchasing intentions of Japanese consumers regarding pork produced by ‘Ecofeed’, a trademark of food‐waste or co‐product animal feeds certified by the Japanese government, were investigated by a questionnaire on the Internet. ‘Ecofeed’ did not elicit specific impressions as compared to domestic, imported, Kurobuta (in Japan), and specific pathogen‐free (SPF) pork. Purchasing intent for ‘Ecofeed’ pork was the second lowest of the five pork products. Knowledge and purchasing experience regarding ‘Ecofeed’ pork was the lowest of the five pork products. Respondents were classified into four categories according to their impressions of ‘Ecofeed’ pork. The largest category of respondents did not have any specific impression of ‘Ecofeed’ pork and had little knowledge of pork farming. A category that had a positive impression for ‘Ecofeed’ pork had high knowledge of the pork farming system. In order to establish ‘Ecofeed’ pork in Japan, our results suggest that information disclosure and education about ‘Ecofeed’, its certification system, environmental benefits and the current self‐efficiency ratio of animal feed, are needed.  相似文献   

Changes in oxidative status in periparturient dairy cows in hot conditions     

Masahito TANAKA  Yuko KAMIYA  Tomoyuki SUZUKI  Yutaka NAKAI 《Animal Science Journal》2011,82(2):320-324

We investigated the changes in the plasma concentrations of oxidative stress markers in periparturient dairy cows in hot conditions. This study was performed on 10 cows from day ?5 to day 10 after parturition. The experimental duration was divided into three periods: period 1 (5 days before parturition); period 2 (5 days after parturition); and period 3 (from day 6 to day 10 after parturition). The average rectal temperature in the cows during the experimental period was more than 39°C. Plasma aspartate aminotransferase activities in periods 2 and 3 were higher than that in period 1 (P < 0.05). Blood urea nitrogen concentration in period 3 was lower than those in periods 1 and 2 (P < 0.05). The total ascorbic acid concentration in the plasma in period 2 was greater than those in periods 1 and 3 (P < 0.05). Thiobarbituric acid reactive substances (TBARS) concentrations in periods 2 and 3 were greater than that in period 1 (P < 0.05). TBARS concentration was positively correlated with aspartate aminotransferase activity (P < 0.05). These results suggested that oxidative stress increased in cows after parturition under hot climatic conditions.  相似文献   

An immunohistochemical study of the gastro-entero-pancreatic endocrine cells in the alimentary tract of the Korean tree squirrel, Sciurus vulgaris corea.   总被引：2，自引：0，他引：2  

H S Lee  Y Hashimoto  Y Kon  M Sugimura 《The Japanese journal of veterinary research》1991,39(2-4):117-131

The regional distribution and relative frequencies of gastrointestinal endocrine cells were studied immunohistochemically in the gastrointestinal mucosa of Korean tree squirrels. Seven kinds of endocrine cells were identified in this study. Although a large number of 5-hydroxytryptamine-immunoreactive cells were seen throughout the gastrointestinal tract, they were most predominant in the duodenum. A moderate number of glucagon-immunoreactive cells which were restricted to the cardia and fundus of the stomach was also observed. Bovine chromogranin-immunoreactive cells were numerous in the cardia and pylorus of the stomach, found in moderate numbers in the fundus, duodenum and large intestine, but rare in the jejunum. Porcine chromogranin-immunoreactive cells were found in moderate numbers in the stomach but were rare in the duodenum. Gastrin/cholecystokinin-immunoreactive cells were abundant in the pyloric gland region but scarce in the duodenum. Bovine pancreatic polypeptide-immunoreactive cells were observed to be rare and found only in the pyloric gland region. Somatostatin-immunoreactive cells were distributed moderately in the stomach but were few in number in the intestine. No insulin-immunoreactive cells were found in the gastrointestinal tract of Korean tree squirrels. These results suggest that although the Korean tree squirrel is a herbivorous rodent, the distribution pattern of its gastro-entero-endocrine cells is rather similar to that reported for omnivorous animals.  相似文献   

Localization of acid phosphatase in chicken juxtaglomerular cells and its functional meaning     

Y Kon  Y Hashimoto  H Kitagawa  N Kudo 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(5):925-931

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Light and electron microscopic studies on chicken intestinal globule leucocytes     

H Kitagawa  Y Hashimoto  Y Kon  N Kudo 《The Japanese journal of veterinary research》1988,36(2):83-117

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Distribution and morphology of the renin-containing cells in the amphibian kidneys     

Y Kon  Y Hashimoto  H Kitagawa  N Kudo 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(2):325-332

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