Sequence variability in the structural protein-encoding region of foot-and-mouth disease virus serotype Asia1 field isolates     

Mohapatra JK  Sanyal A  Hemadri D  Tosh C  Sabarinath GP  Manoj Kumar R  Venkataramanan R  Bandyopadhyay SK 《Research in veterinary science》2004,77(2):153-161

A total of 30 field isolates of foot-and-mouth disease virus (FMDV) serotype Asia1 belonging to two different lineages and five isolates belonging to a divergent group as delineated earlier in 1D (encodingVP1 protein) gene-based phylogeny were sequenced in the structural protein (P1) coding region. Phylogenetic comparison of these isolates along with some of the published exotic sequences revealed the presence of five different lineages around the world. Similar grouping pattern was observed for the P1 region and 1D gene-based phylogeny, where the Indian isolates were clustered in two genetic lineages. The recently identified divergent group of virus falls into a separate sub-cluster. Similar grouping was also observed in L gene-based phylogeny. Comparison of amino acid sequences identified lineage-specific signature residues in all the structural proteins. Comparison of Asia1 field isolates at the identified key residues of other FMD viruses involved in the formation of the heparan sulfate-binding ligand confirmed many of them to be conserved and the presence of VP3(56) Arg suggested their cell culture adaptation. Although a considerable genetic variation was observed among the isolates of present study, all of them tested in micro-neutralization test were serologically related to the vaccine strain.  相似文献   

Identification of genes preferentially expressed in testis and spermatogonial cells of Labeo rohita by subtractive and suppressive hybridization     

Chinmayee Mohapatra  Aliza Swain  Ambekar E Eknath 《Aquaculture Research》2011,42(8):1196-1205

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An economic analysis of urban infrastructure development project in the northern Indian city of Bharatpur     

Dipti Ranjan Mohapatra 《Growth and change》2019,50(3):1134-1163

Rajasthan Urban Sector Development Program involved investment in water supply, sewerage, sanitation, and drainage, solid waste management (SWM), and etc. in major cities in the state of Rajasthan in India. This program was an initiative of the Government of Rajasthan and the Government of India carried out with a loan arrangement from Asian Development Bank. The phase II of the program was implemented in 15 cities of Rajasthan including Bharatpur with total investment of 1868.40 million Indian Rupees. The objective of this paper is to evaluate the economic viability of the infrastructure investments in Bharatpur in water supply, sewerage, sanitation, and drainage, SWM, and measure the impact on urban poor. Water supply, sewerage, sanitation, and drainage, and SWM subprojects are economically viable with internal rate of return (IRR) of 85.72%, 22.87%, and 39.34%, respectively. The sensitivity analysis shows above 12% IRR for all the above‐mentioned subprojects. The distribution of project benefits to the urban poor in Bharatpur is significant with poverty impact ratio of 9%.  相似文献   

Purification of cattle oviduct specific proteins and their effect on in vitro embryo development     

S.K. Das  Aditya K. SharmaSushil K. Mohapatra  Vishu BhatiaA. Chatterjee  A.K. Mohanty 《Livestock Science》2013,152(1):88-93

The purpose of this study was to determine the effect of oviduct specific proteins as a media supplement for in vitro embryo development in cattle. The proteins were extracted from oviducts of cows and precipitated by ammonium sulfate (30%, 40%, 50% and 60%) followed by dialysis in 50 mM Tris–HCl (pH 7.0) buffer. The dialyzed proteins were fractionated into acidic, basic and neutral fractions using SP sephadex cation exchange and DEAE sephadex anion exchange column chromatography respectively. Cow oviduct specific proteins (cOSPs) constituting all the extracted proteins were used as media supplement in three different concentrations (10, 50 and 100 μg/ml) for in vitro maturation, fertilization and culture (IVMFC) of cow oocytes. Acidic, basic and neutral (unbound) fractions were also used as media supplement in three different concentrations (10, 30 and 50 μg/ml) for IVMFC. Cumulus oocytes complexes were collected from slaughterhouse ovaries, washed thoroughly and cultured in maturation media for 24 h in 5% CO₂ at 38.5 °C with maximum humidity. In vitro matured oocytes were co-incubated with in vitro capacitated sperm in Fert-BO media at 38.5 °C for 18 h in 5% CO₂. The fertilized oocytes were washed and cultured in embryo development media for cleavage. After 40–42 h cleavage was observed and embryos were put in the replacement media for further development. The cleavage rates (%) for cOSPs were observed as 68.24±2.46, 69.28±2.05, 61.77±0.93 and 42.62±1.31 at concentrations of 0, 10, 50 and 100 μg/ml respectively. Rates of blastocyst stage development were 14.49±3.61, 21.17±2.77, 14.66±1.06 and 11.98±1.84. These results indicate that addition of cOSP at10 μg/ml increased blastocyst formation as compared to other concentrations (0, 50 and 100 μg/ml). Although acidic, basic and neutral fractions seemed to have no major effect on cleavage rate, but both acidic and neutral fraction of oviduct specific proteins improved the cleavage rate at 30 μg/ml concentration and basic fraction improved the blastocyst formation at 10 μg/ml concentration.  相似文献   

Identification of novel quantitative trait loci associated with brown planthopper resistance in the rice landrace Salkathi     

Sangram Keshori Mohanty  Rudraksh Shovan Panda  Soubhagya Laxmi Mohapatra  Arundhati Nanda  Lambodar Behera  Mayabini Jena  Rabindra Kumar Sahu  Sarat Chandra Sahu  Trilochan Mohapatra 《Euphytica》2017,213(2):38

The brown planthopper (BPH) is a potent pest of rice in Asia and Southeast Asia. Host resistance has been found to be the most suitable alternative to manage the insect. But varietal resistance has been found to be short-lived. There has been a constant search for alternate resistance genes. We developed an F₈ recombinant inbred population for the BPH resistance gene in Salkathi, an indica landrace from Odisha, India. Phenotyping of RILs against the BPH population at Cuttack, Odisha showed continuous skewed variation with four peaks at 2.1–3.0, 4.1–5.0, 6.1–7.0 and 8.1–9.0 SES score, suggesting the involvement of quantitative loci for resistance to BPH in Salkathi. Mapping showed the presence of two QTLs on the short arm of chromosome 4. One QTL, with phenotype variance of 37.02% is located between the markers RM551 and RM335. The other QTL, with phenotype variance of 7.1% is located between markers RM335 and RM5633. The two QTLs have been designated as qBph4.3 and qBph4.4. QBph4.3 seems to be a novel QTL associated with BPH resistance. We have successfully transferred qBph4.3 and qBph4.4 into two elite rice cultivars, Pusa 44 and Samba Mahsuri. Fine mapping of the identified QTLs may lead to a successful transfer of QTLs into other elite germplasm backgrounds.  相似文献   

Dietary Multispecies Probiotic Supplementation Enhances the Immunohematological Responses and Reduces Mortality by Aeromonas hydrophila in Labeo rohita Fingerlings     

Sipra Mohapatra  Tapas Chakraborty  Ashisa Kumar Prusty  Kurucheti Pani Prasad  Kedar Nath Mohanta 《Journal of the World Aquaculture Society》2014,45(5):532-544

A study was conducted to determine the immunomodulatory effect of probiotic mixture on the nonspecific immune response and disease resistance of Labeo rohita. Fish were fed with six different test diets with or without probiotics, namely, T1 (basal feed [BF] without probiotics), T2 (BF + Bacillus subtilis [BS] + Lactococcus lactis [LL]), T3 (BF + LL + Saccharomyces cerevisiae [SC]), T4 (BF + BS + SC), T5 (BF + BS + LL + SC), and T6 (BF + heat‐killed bacteria of BS + LL + SC). During the prechallenge period, significantly higher (P < 0.01) nitroblue tetrazolium assay (NBT), red blood cells (RBC), hemoglobin (Hb), globulin, and albumin content were recorded in the probiotic‐supplemented diet group. Feeding all the three probiotics significantly decreased (P < 0.01) the serum aspartate aminotransferases (AST), alanine aminotransferases (ALT), acid phosphatase (ACP), and alkaline phosphatase (ALP) activity and increased the myeloperoxidase (MPO) and immunoglobulin M (IgM) value. Significant difference in the NBT, Hb, serum albumin, and MPO was noticed in both prechallenged and postchallenged fish. Lowest survivability was recorded in the nonprobiotic fed group, followed by the group fed with heat‐killed probiotics, whereas the highest survivability was noticed in the group fed with all the three probiotics in live form. Thus, a probiotic mixture diet constituting of three probiotics in viable form is found to be optimum to enhance the immunity and postinfection survivability in L. rohita fingerlings.  相似文献   

Utilization of gelatinized carbohydrate in diets of Labeo rohita fry     

M. Mohapatra  N.P. Sahu  & A. Chaudhari 《Aquaculture Nutrition》2003,9(3):189-196

A total of 180 Labeo rohita fry (≈1 g) were distributed into six treatment groups with three replicates each. Six isoenergetic (15.8 kJ g^?1) diets were prepared with varying levels of protein (CP) and gelatinized carbohydrate (GC), viz. D₁: 450 g kg^?1 CP, 258 g kg^?1 GC; D₂: 400 g kg^?1 CP, 302 g kg^?1 GC; D₃: 350 g kg^?1 CP, 399 g kg^?1 GC; D₄: 301 g kg^?1 CP, 447 g kg^?1 GC; D₅: 249 g kg^?1 CP, 517 g kg^?1 GC and control diet, D₆: 400 g kg^?1 CP, 468 g kg^?1 GC and these were fed to fish at 40 g kg^?1 wet body weight per day for 60 days. Highest growth was recorded for fish fed D₄, decreasing for D₅. Protein utilization, amylase activity and carbohydrate digestibility were significantly positively correlated with GC (P < 0.05) and were highest in fish fed D₅. Hypertrophy of hepatic cells was only recorded for D₅. Lysine, which may be affected during gelatinization, was estimated in diets and found to be above the minimum level required. Survival rate was unaffected by diet. The results of the present study indicate that a diet containing 450 g kg^?1 GC and 300 g kg^?1 CP was efficiently utilized by L. rohita fry.  相似文献   

Effect of 2-(alpha-hydroxybenzyl) benzimidazole (HBB) 5-iodo-2'-deoxyuridine (IUD) and actinomycin D on replication of strains of bovine enterovirus     

N Nath  S Balaya  L N Mohapatra 《The Indian veterinary journal》1971,48(1):1-5

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Molecular fingerprinting of hybrids and assessment of genetic purity of hybrid seeds in rice using microsatellite markers   总被引：2，自引：0，他引：2  

N. Nandakumar  A.K. Singh  R.K. Sharma  T. Mohapatra  K.V. Prabhu  F.U. Zaman 《Euphytica》2004,136(3):257-264

Microsatellite markers were used for fingerprinting of hybrids, assessing variation within parental lines and testing the genetic purity of hybrid seed lot in rice. Ten sequence tagged microsatellite sites (STMS) markers were employed for fingerprinting 11 rice hybrids and their parental lines. Nine STMS markers were found polymorphic across the hybrids and produced unique fingerprint for the 11 hybrids. A set of four markers (RM 206, RM 216, RM 258 and RM 263) differentiated all the hybrids from each other, which can be used as referral markers for unambiguous identification and protection of these hybrids. Cluster analysis based on Jaccard's similarity coefficient using UPGMA grouped the hybrids into three clusters. Within the cluster all the hybrids shared a common cytoplasmic male sterile line as female parent. The genetic similarity between the hybrids ranged from 0.33 to 0.92 with an average similarity index of 0.63. The analysis of plant-to-plant variation within the parental lines of the hybrid Pusa RH 10, using informative markers indicated residual heterozygosity at two marker loci. This highlights the importance of STMS markers in maintaining the genetic purity of the parental lines. The unique value of the restorer gene linked marker for testing the genetic purity of hybrid seeds is demonstrated for the first time.  相似文献   

A stable cytoplasmic male-sterile line of Brassica juncea carrying restructured organelle genomes from the somatic hybrid Trachystoma ballii+B. juncea   总被引：2，自引：0，他引：2  

P. B. Kirti    T. Mohapatra    A. Baldev    S. Prakash  V. L. Chopra 《Plant Breeding》1995,114(5):434-438

A cytoplasmic male-sterile (CMS) line of Brassica juncea has been developed by combining the cytoplasm originating from the somatic hybrid Trachystoma ballii+B. juncea, and the nucleus of B. juncea cv. Pusa Bold by repeated backcrossing. Male-sterile plants closely resembled the normal fertile B. juncea in general morphology, but had delayed flowering (5–7 days) when compared with fertile ‘Pusa Bold’ which flowered in 45 days. Stamens of the male-sterile line were transformed into petaloid structures. Pollen abortion occurred after tetrad formation. Female fertility of the male-sterile line was normal. Molecular analysis of organelle genomes indicated extensive mitochondrial DNA recombinations in the CMS line. Preliminary analysis of the chloroplast genome of the CMS line also indicated chloroplast DNA recombination.  相似文献