Isolation and characterization of lipopolysaccharides from different rhizobial isolates     

Mahipal Singh Kesawat  Basanta Kumar Das  G. R. Bhaganagare  Vinay Sharma  Manorama 《Journal of Crop Science and Biotechnology》2009,12(3):109-113

Formation of nodules on roots or in stems (in some cases) of leguminous plants is the unique ability of gram-negative bacteria, Rhizobia, which converts atmospheric nitrogen into usable forms by the host plant. Lipopolysaccharide (LPS) is the outer membrane component of the gram-negative bacteria, known to be an essential factor in host recognition, specificity, and initial infection processes. In the present study, we extracted lipopolysaccharides from different rhizobial isolates by a modified phenol-water method and partially characterized by polyacrylamide gel electrophoresis with silver staining. The results showed two separate banding regions, LPS-I and LPS-II. The high molecular weight and electrophoretic mobility of LPS-I region resembles that of lysozyme, used as a standard marker. The LPS-II region has a low molecular weight and electrophoretic mobility greater than that of lysozyme. The LPS-II region was due to incomplete LPS, which either lacks the entire O-antigen repeating unit or contains only one or two repeating units. The banding patterns of LPS vary among the different rhizobial isolates. Results revealed that the type of LPS structure and banding regions greatly facilitate the further characterization of the LPS modifications required for symbiosis.  相似文献   

Surgical repair of intermandibular fistula with protrusion of the tongue in a buffalo     

Saini NS  Anand A  Bansal PS  Mahajan SK  Bishnoi P 《The Veterinary record》2002,150(20):638-639

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Hemocyte diversity of the American bollwormHelicoverpa armigera     

Govind T. Gujar  Vinay K. Kalia 《Phytoparasitica》2005,33(1):17-27

The hemocytes of the American bollwormHelicoverpa armigera (Hübner) were studied in the last instar larvae by phase contrast microscopy to investigate the differences among 14 populations, collected from ten locations, spread over a distance of approximately 3000 km in six different states of India. The cluster analysis of differential hemocytes showed as many as 11 clusters of populations at 5% and six clusters at 10% homogeneity level. The cluster analysis of hemocytes of seven populations from cotton crops showed five and three clusters at 5% and 10% homogeneity levels, respectively. Diversity of hemocytes of four populations from chickpea was wider than that from cotton. The differences in hemocytes of various populations may be due to space, time, host plant, insecticide use, other agroecological conditions, and insect genetic variabilityper se. The field populations ofH. armigera heavily treated with insecticides appeared to contain more spherulocytes at the expense of plasmatocytes and granulocytes. The treatment of 6-day-old larvae ofH. armigera with cypermethrin decreased plasmatocytes and granulocytes, and increased prohemocytes and spherulocytes in the hemolymph of last instar larvae. Thus, differential hemocyte counts may serve as an indicator of insecticide exposure and the diversity of insect populations. http://www.phytoparasitica.org posting Dec. 20, 2004.  相似文献   

DIAGNOSTIC CHALLENGE     

Geoffrey R. Browning  David Eshar  Vinay Shivanna  Giselle Cino 《Journal of Exotic Pet Medicine》2018,27(3):50-53

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Angioinvasive lymphoma (lymphomatoid granulomatosis) in a cat,with cutaneous and ocular metastasis     

Rebecca Bacon  Vinay Shivanna  Misty Gore  Jamie Henningson  Charan Ganta 《Journal of veterinary diagnostic investigation》2021,33(2):340

Lymphomatoid granulomatosis (LYG) is a rare variant of an angioinvasive T-cell lymphoproliferative disorder that primarily affects the lungs, with common sites of metastasis including the skin and subcutis. In humans, it is a B-cell lymphoproliferative disorder associated with Epstein-Barr virus infection. Our case is a 7-y-old, spayed female, domestic longhair cat that decompensated and was euthanized following an initial diagnosis of angioinvasive lymphoma from a skin biopsy. Autopsy revealed nodules in the lungs and subcutis, and corneal thickening and cloudiness. Histologic examination of cutaneous nodules, lungs, and eye showed similar angioinvasive cellular infiltrates and pattern to that of the original skin biopsy, consistent with a diagnosis of LYG. The neoplastic cells displayed CD3-positive immunoreactivity in the skin, eye, and lung, and PCR for antigen receptor rearrangement (PARR) showed T-cell clonality in all tissues tested. This is the third case of LYG to be reported in cats and is the only case in which PARR analysis and immunophenotyping immunohistochemical staining was performed. LYG with ocular involvement has not been reported previously in cats, to our knowledge. Our case demonstrates the necessity for considering LYG when presented with a cat with respiratory signs in conjunction with subcutaneous nodules and ocular lesions.  相似文献   

Complement test for distinctiveness,uniformity and stability testing of kharif onion (Allium cepa L.) varieties     

Gupta  Amar Jeet  Anandhan  S.  Manjunathagowda  Dalasanuru Chandregowda  Benke  Ashwini Prashant  Mahajan  Vijay  Kad  Snehal K.  Singh  Major 《Genetic Resources and Crop Evolution》2022,69(6):2217-2229

Genetic Resources and Crop Evolution - The distinctiveness, uniformity and stability (DUS) testing is important for varietal identification and protection of plant variety. It has become one of the...  相似文献   

Molecular characterization of short-day onion genotypes by intron length polymorphic (ILP) markers     

Khade  Yogesh Popat  Salunkhe  Shubham Rajaram  Manjunathagowda  Dalasanuru Chandregowda  Sinhasane  Shalaka Ramling  Mahidar Gowd  Talamarla Yeswanth  Mahajan  Vijay  Singh  Major 《Genetic Resources and Crop Evolution》2022,69(6):2077-2086

Genetic Resources and Crop Evolution - Onion (Allium cepa L.) is an economically important vegetable that is in high demand around the world. It is a highly cross pollinated crop, which could...  相似文献   

Analysis of water vapour transmission rate of perforation-mediated modified atmosphere packaging (PM-MAP)     

P.V. Mahajan  F.A.S. Rodrigues  E. Leflaive 《Biosystems Engineering》2008,100(4):555-561

Perforation-mediated modified atmosphere packaging relies on the use of perforations (tubes) of different dimensions to control O₂ and CO₂ exchange in order to create the desired atmosphere for preservation of fresh fruits and vegetables. No work has been done so far to measure the water vapour transmission rate (WVTR) of this new system. The present study analyses WVTR in different perforation dimensions (diameter: 9, 13 and 17 mm; length: 10, 20 and 30 mm) and storage temperatures (4, 10 and 16 °C). Diameter was the variable with the greatest influence on WVTR, followed by tube length and temperature. The WVTR of perforation-mediated packages increased with increasing temperature and tube diameter while it decreased with increasing tube length. A mathematical model to describe the changes in WVTR as a function of perforation diameter, length, porosity and storage temperature was developed and validated successfully at 7 °C. An experiment with mushrooms showed that the perforation-mediated modified atmosphere packaging could be used for fresh produce provided that the condensation is minimised by using a moisture absorber.  相似文献   

Draft Genome Sequencing of <Emphasis Type="Italic">Rhizoctonia solani</Emphasis> Anastomosis Group 3 (AG3- PT) Causing Stem Canker and Black Scurf of Potato     

Virupaksh U. Patil  Vanishree Girimalla  Vinay Sagar  Vinay Bhardwaj  S. K. Chakrabarti 《American Journal of Potato Research》2018,95(1):87-91

Rhizoctonia solani is a soil-borne basidiomycete fungus with a necrotrophic lifestyle being classified into fourteen reproductively incompatible anastomosis groups (AGs). AG3-PT (a potato subgroup) is associated with quantitative and qualitative yield losses through stem canker and black scurf in potato. Here we present the first draft sequence of the R. solani [AG3-PT] strain RS-20 with a G-C content of 48.3%. It consists of 11,431 total predicted protein coding regions including 181 tRNA and 31 rRNA coding genes. The initial pBLAST revealed more than 97% hits among AG groups where as only 1.7% of genes hit with other organisms. The R. solani genome is found to be dominated with tri mer repeats. The genome-wide evolutionary studies revealed the close association of AG3-PT with AG3. The draft sequence represents a helpful resource not only for understanding the core genes involved in pathogenecity but also evolution and adaptive behaviour within the R. solani species complex.  相似文献   

Traditional processing treatments as a promising approach To enhance the functional properties of rapeseed (Brassica campestris var. toria) and sesame seed (Sesamum indicum) meals.     

A Mahajan  S Bhardwaj  S Dua 《Journal of agricultural and food chemistry》1999,47(8):3093-3098

Different processing treatments were applied to rapeseed and sesame seed meals, and the functional properties of these products were assessed. All treatments except puffing for both meals and pressure cooking in sesame meal increased water absorption capacity (WAC). Fat absorption capacity (FAC) of rapeseed meals was enhanced significantly by all treatments. The full-fat meals of both sources showed maximum protein solubility when fermented and minimum protein solubility when pressure-cooked. Germinated and microwave-cooked meals enhanced foaming properties of rapeseed meals. Heat treatments, except microwave cooking, considerably reduced emulsifying properties of both meals. Fermentation and germination increased the specific viscosity of rapeseed meals, whereas processed sesame meals showed lower viscosity than dry sesame meals.  相似文献