Marker‐assisted breeding of Pi‐1 and Piz‐5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH‐10 Basmati rice hybrid     

Patil K. Gouda  Surapaneni Saikumar  Chejerla M. K. Varma  Kancharla Nagesh  Sanka Thippeswamy  Vinay Shenoy  Mugalodim S. Ramesha  Halagappa E. Shashidhar 《Plant Breeding》2013,132(1):61-69

Rice blast, caused by fungus Magnaporthe grisea, is a serious disease causing considerable economic damage worldwide. Best way to overcome disease is to breed for disease‐resistant cultivars/parental lines of hybrids. Pusa RH10, first aromatic, fine‐grain rice hybrid released and cultivated extensively in India. Hybrid and its parental lines, Pusa 6A and PRR78, are highly susceptible to blast. CO39 pyramid carrying two dominant, broad‐spectrum blast‐resistance genes, viz. Pi‐1 and Piz‐5, used as a donor parent to introgress these genes into PRR78 using marker‐assisted backcrossing (MABC). Microsatellite markers RM5926 and AP5659‐5 tightly linked to Pi‐1 and Piz‐5 genes, respectively, were used for foreground selection to derive introgression lines. Further, these lines were evaluated for agronomic performance, disease reaction and cooking quality traits along with PRR78. Most of the improved lines were on par with PRR78 for all traits evaluated except gelatinization temperature. Recurrent parent genome percentage (RPG) study also revealed similarity of these lines with PRR78. Hybrids derived using improved PRR78 lines were superior over Pusa RH10 in terms of yield.  相似文献   

Making an Indian traditional rice variety Mahsuri, bacterial blight resistant using marker-assisted selection     

Lalitha Devi Guvvala  Pranitha Koradi  Vinay Shenoy  Lalitha Shanti Marella 《Journal of Crop Science and Biotechnology》2013,16(2):111-121

Mahsuri a popular traditional variety and the first rain-fed mega variety of the Indian sub- continent. It is highly susceptible to bacterial leaf blight caused by Xanthomonas oryzae pv.oryzae. Nine best performing families of Mahsuri pyramid containing four bacterial blight resistance genes (Xa4, xa5, xa13 and Xa21) were evaluated for agronomic, yield and its related characters viz. days to 50% flowering, plant height, number of tillers, panicle length, filled grains per panicle, grain weight, and yield under natural and disease pressure conditions for three consecutive wet seasons. In addition these pyramids were also evaluated for three different spacings to find out the optimum spacing under disease free and disease pressure conditions. Results revealed that under disease free conditions there was no significant difference between the pyramids and the parent for the characters evaluated in each spacing. However characters plant height, number of tillers, panicle length, filled grains per panicle, yield per plant and yield per sq. m. showed significant variation between the different spacings across seasons. Under heavy disease pressure the parent exhibited highly susceptible reaction whereas the pyramid families were highly resistant. A wider spacing had less yield loss when compared to dense planting under BB infestation in case of parent. There was no such yield loss in the pyramid families. When yield per sq. m. was taken into consideration the 20 × 20 cm spacing showed the highest yield when compared to the other two spacings since number of plants were more. The pyramids insulated the yield loss against bacterial leaf blight and are a gain to the farmers to help overcome the heavy yield losses due to this disease. These pyramids have the potential to replace the parent and can be used directly. In addition they can be used as donors for bacterial blight resistance in any breeding program.  相似文献   

Surgical retrieval of a metallic foreign body from the spleen of a dog     

Shashi K. Mahajan  Arun Anand  Vandana Sangwan  Jitender Mohindroo  Kiranjit Singh 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(4):399-401

This is the first report of a metallic foreign body in the spleen of a dog. The animal had abdominal discomfort with a tucked-up abdomen and occasional vomition. Radiography and ultrasonography were used to identify a splenic foreign body which was surgically removed through a ventral midline celiotomy. Migration of the foreign body through the stomach wall was suspected as the most likely cause.  相似文献   

Diversity analysis of Tomato leaf curl New Delhi virus-[potato], causing apical leaf curl disease of potato in India     

Arjunan Jeevalatha  Swarup Kumar Chakrabarti  Sanjeev Sharma  Vinay Sagar  Kamlesh Malik  Baswaraj Raigond  Bir Pal Singh 《Phytoparasitica》2017,45(1):33-43

The complete coat protein (cp) gene sequence of eighty Tomato leaf curl New Delhi virus-[potato] (ToLCNDV-[potato]) isolates collected from eleven states were determined. Phylogenetic analysis based on cp gene grouped the isolates into two major clades (I & II) and they shared 95.9–100.0% identity. The DNA A and DNA B of eight representative isolates (six from clade I and two from clade II) were 2739–2740 and 2692–2694 nts long and shared 94.6–99.4% and 97.2–99.5% homology within the isolates, respectively. Among the eight isolates, the DNA A of two isolates (Clade II), GWA-5 and FAI-19 had 94.6–95.3% sequence identity to other six isolates and formed a sub-clade within the ToLCNDV-[potato] isolates. Similar grouping was also revealed with AC1 and AC4 genes of these eight isolates. The DNA A components shared more than 90.0% identity with the DNA A of ToLCNDV isolates from cucurbitaceous crops, tomato, bhendi, 89.0–90.0% with ToLCNDV-papaya isolates and 70.4–74.0% with other tomato leaf curl viruses. Hence, the begomovirus infecting potatoes are the ToLCNDV isolates, designated as ToLCNDV-[potato]. Whereas, the DNA B components shared 86.6–91.7% identity with ToLCNDV isolates from cucurbits, tomato and bhendi. Evidence for intra-species recombination was detected only in DNA A with a maximum of three events in GWA-5 and FAI-19 isolates. Analysis of cp gene, DNA A, iterons and recombination events clearly indicate that two groups of ToLCNDV-[potato] infects potato in India.  相似文献   

Chlamydial Group Antigen: Its Preparation and Use in complement Fixation Test for Measuring Chlamydial Group Antibodies in Animals     

P. N. Dhingra  L. P. Agarwal  V. M. Mahajan  S. C. Adlakha  K. K. Baxi 《Zoonoses and public health》1981,28(4):336-340

Chlamydien Gruppentantigen: Seine Herstellung und Anwendung in der Komplementbindungsreaktion zur Messung des Antikörpergehalts in Tieren gegen die Chlamydiengruppe Ein hochwirksames, ätherextrahiertes Gruppenantigen (Titer 1:256) wurde hergestellt. Benutzt wurde Stamm Bour von Chlamydia Trachomatis. Bei dieser Technik werden nur solche Einrichtungen benötigt, wie sie in jedem Routinelabor zu finden sind. Insgesamt wurden 886 Seren von gesund erscheinenden Rindern, Büffeln, Schafen und Ziegen (etwa 200 Stück pro Tierart) mit Hilfe der KBR gegen das Chlamydien-Gruppenantigen getestet. Serologisch positiv reagierten 53% der Rinder, 44% der Büffel, 52,9% der Ziegen und 38% der Schafe. Es wurden Titer zwischen 1:4 und 1:64 beobachtet. Die Verbreitung der Gruppen-Antikörper bei diesen Tieren, die keine offensichtliche Erkrankung zeigen, wird diskutiert.  相似文献   

Comparison of seroreactivity of lentogenic, mesogenic and velogenic strains of Newcastle disease virus by enzyme linked immunosorbent and haemagglutination inhibition assays on human sera     

S Charan  V M Mahajan  A Rai 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1983,30(1):55-60

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Experimental toxoplasmosis in buffalo calves     

O.P. Gautam  M.B. Chhabra  S.L. Gupta  S.K. Mahajan 《Veterinary parasitology》1982,11(4):293-299

Eight buffalo calves were inoculated with infectious oocysts of a virulent strain of Toxoplasma gondii, five with 1 × 10⁵, and the other three with 5 × 10⁵ oocysts each while two more were kept as non-inoculated controls. Infected animals developed pyrexia, anorexia, conjunctivitis and dyspnoea. The clinical symptoms, haematological changes and serological response were of a low order. The appearance of haemagglutinating antibodies at significant levels (? 1:64) was first observed at 21 days after inoculation (DAI), peaked at 42 DAI and rapidly declined after 63 DAI. The inoculated calves were killed (or died) at intervals from 11 DAI to 110 DAI. The organism could be recovered from several times of a calf that died 11 DAI, but was rapidly removed subsequently. Only the lymph nodes were infective till 32 DAI. Parasitism of the retina was demonstrated in one calf. The significance of these findings is discussed.  相似文献   

Pathogenesis of Newcastle disease virus in cell-mediated immune response deprived mice     

S Charan  V M Mahajan 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1983,30(6):462-465

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Influence of reproductive phase on the inflammatory response and rate of sperm removal in the uterus and oviduct of the cow     

S C Mahajan  A C Menge 《American journal of veterinary research》1967,28(125):1037-1041

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Study on body weight and conformation of Gaddi and Romney Marsh sheep     

A K Thakur  J M Mahajan 《The Indian veterinary journal》1967,44(7):589-595

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