QTL Analysis of Late Blight Resistance in a Diploid Potato Family of Solanum spegazzinii × S. chacoense     

S. K. Chakrabarti  B. P. Singh  Garima Thakur  Jagesh K. Tiwari  S. K. Kaushik  Sanjeev Sharma  Vinay Bhardwaj 《Potato Research》2014,57(1):1-11

Several quantitative trait loci (QTL) for resistance to late blight have been reported in diploid potatoes. The diploid wild potato species Solanum chacoense possesses a high degree of horizontal resistance to late blight. In the present study, we report on QTL mapping for late blight resistance in a diploid mapping population of 126 F₁ of Solanum spegazzinii (susceptible) × S. chacoense (resistant). The area under the disease progress curve (AUDPC) values for late blight resistance using the “whole plant in vitro assay” and the “detached leaf assay” on the mapping population displayed quantitative variation. Out of 64 AFLP primer-pairs combinations and SSR markers, a total of 209 significant AFLP loci were placed onto the 12 linkage group of potato covering a total map length of 6548.1 cM. QTL analysis based on the AUDPC dataset of the “whole plant in vitro assay” using the interval-mapping option identified two QTL (LOD?>?2.5) located on linkage groups IX and X, which explained 14.70 and 3.40% variation, respectively. The present study revealed the presence of potential new genetic loci in the diploid potato family contributing to quantitative resistance against late blight.  相似文献   

Effects of nematicides on the chemical composition of the fruits of egg-plant (Solanum melongena L.)     

K. L. Bajaj  R. Mahajan 《Plant foods for human nutrition (Dordrecht, Netherlands)》1980,30(1):69-72

Five nematicides — aldicarb, carbofuran, phorate, fensulfothion and DBCP — were studied in a field experiment to investigate their effect on the chemical composition of the fruits of egg-plant. There were no significant differences in the dry matter content of fruits from different treatments. A significant decrease in the crude protein content was observed with nematicide treatments. Phenolic compounds increased on application of phorate while all other treatments, except carbofuran, decreased these compounds. A significant increase over control in solasodine content of fruits was observed with all treatments.  相似文献   

Line × tester analysis of combining-ability and heterosis for some economic characters in okra     

B.R. Sharma  Yash Paul Mahajan 《Scientia Horticulturae》1978,9(2):111-118

In okra (Abelmoschus esculentus (L) Moensch), heterosis over the mid-parental values was manifested in all the characters studied. However, overdominance was observed only for days to first flowering, fruit length, plant height, fruit weight and yield per plant. Among the testers, ‘Pusa Sawani’ was found to be the best general combiner for yield and most other characters, whereas ‘Pusa Sawani’ (Bangalore lot-NSC) and ‘Crimson Spineless’ were observed to be high general combiners for yield and other desirable attributes amongst the females. Gene action based on the general and specific combining ability variances for various characters was found to be mainly non-additive.‘Pusa Sawani’ (Bangalore lot-NSC) × ‘Smooth Long Green’ was found to be a good cross combination for number of fruits per plant and fruit yield. Another good combination for plant height and fruit yield was ‘American Seven Dhari’ × ‘Pusa Sawani’.  相似文献   

Experimental Sarcocystis levinei infection in buffalo calves     

A K Jain  S L Gupta  R P Singh  S K Mahajan 《Veterinary parasitology》1986,21(1):51-53

Six buffalo calves were orally inoculated with 3 graded doses of sporocysts of Sarcocystis levinei (0.5, 1.0 and 2.0 million sporocysts; 2 calves for each dose) while two more calves were kept as uninoculated controls. One calf from each group was killed at 30 days post infection (DPI) and the other at 80 DPI. Inoculated calves showed a dose dependent response. The calves inoculated with 0.5 and 1.0 million sporocysts did not manifest any clinical signs of disease up to 80 DPI. One of the two calves inoculated with 2.0 million sporocysts showed clinical signs of weakness, emaciation and anaemia during the 5th week post infection. The other calf remained healthy until it was killed at 30 DPI. Pale liver tissue, gelatinization of fat and haemorrhages in the heart were observed in one calf inoculated with 2.0 million sporocysts; only microscopic lesions were seen in other calves. Schizonts and merozoites were not observed in any calf. Mature sarcocysts were observed in cardiac and skeletal muscle of calves killed at 80 DPI whereas no sarcocysts were seen in calves killed at 30 DPI.  相似文献   

Recombinant origin of the retrovirus XMRV     

Paprotka T  Delviks-Frankenberry KA  Cingöz O  Martinez A  Kung HJ  Tepper CG  Hu WS  Fivash MJ  Coffin JM  Pathak VK 《Science (New York, N.Y.)》2011,333(6038):97-101

The retrovirus XMRV (xenotropic murine leukemia virus-related virus) has been detected in human prostate tumors and in blood samples from patients with chronic fatigue syndrome, but these findings have not been replicated. We hypothesized that an understanding of when and how XMRV first arose might help explain the discrepant results. We studied human prostate cancer cell lines CWR22Rv1 and CWR-R1, which produce XMRV virtually identical to the viruses recently found in patient samples, as well as their progenitor human prostate tumor xenograft (CWR22) that had been passaged in mice. We detected XMRV infection in the two cell lines and in the later passage xenografts, but not in the early passages. In particular, we found that the host mice contained two proviruses, PreXMRV-1 and PreXMRV-2, which share 99.92% identity with XMRV over >3.2-kilobase stretches of their genomes. We conclude that XMRV was not present in the original CWR22 tumor but was generated by recombination of two proviruses during tumor passaging in mice. The probability that an identical recombinant was generated independently is negligible (~10(-12)); our results suggest that the association of XMRV with human disease is due to contamination of human samples with virus originating from this recombination event.  相似文献   

Deconstructing molecular phylogenetic relationship among cultivated and wild Brassica species     

Sudan  Jebi  Singh  Ravinder  Mahajan  Reetika  Salgotra  Romesh K. 《Genetic Resources and Crop Evolution》2021,68(6):2281-2288

Genetic Resources and Crop Evolution - Brassica represents an agriculturally important and diverse group of oilseed crops with a long evolutionary history. Molecular markers played an important...  相似文献   

Isolation of Chlamydia from Pneumonic Lungs of Buffaloes,Cattle and Sheep     

P. N. Dhingra  L. P. Agarwal  V. M. Mahajan  S. C. Adalakha 《Zoonoses and public health》1980,27(8):680-682

  相似文献   

Parent—Offspring correlations and heritability of some characters in okra     

Yash Paul Mahajan  B.R. Sharma 《Scientia Horticulturae》1979,10(2):135-139

Four testers, 16 lines and 64 F₁'s were used in a line × tester analysis of okra (Abelmoschus esculentus L.). Parent—offspring correlations were high for yield per plant, number of ridges per fruit and fruit diameter with all the 4 testers. The heritability estimates were high for number of fruits, fruit length and fruit diameter. Yield had a positive and significant association with plant height, number of fruits per plant and fruit length, both in parents and hybrids. It was, therefore, suggested that number of fruits per plant and fruit length and diameter be given more consideration in selecting superior types in okra.  相似文献   

Dravya,a product of seaweed extract (<Emphasis Type="Italic">Sargassum wightii</Emphasis>), induces resistance in cotton against<Emphasis Type="Italic">Xanthomonas campestris</Emphasis> pv.<Emphasis Type="Italic">malsvacearum</Emphasis>     

Vinay B. Raghavendra  S. Lokesh  H. S. Prakash 《Phytoparasitica》2007,35(5):442-449

Dravya, a commercially developed aqueous seaweed extract, was evaluated for its effect on the expression of symptoms of bacterial blight caused byXanthomonas campestris pv.malvacearum (E.F. Smith) Dye in cotton. Seed soaking with Dravya (1:500) followed by foliar spray thrice at intervals of 10 days (10, 20, 30 days after sowing) resulted in a reduction in blight incidence on plants by 66%, 70% and 74% as determined 40, 60 and 80 days, respectively, after sowing. Induction of systemic resistance was associated with increases in plant height, total number of bolls formed, boll weight, stem girth, chlorophyll content, total phenols and peroxidase activity, which intimates that Dravya could be used as an ecofriendly potential input in the integrated management of bacterial-blight of cotton.  相似文献   

Single-slice dynamic computed tomographic determination of glomerular filtration rate by use of Patlak plot analysis in anesthetized pigs     

Alexander K  Del Castillo JR  Ybarra N  Morin V  Gauvin D  Authier S  Vinay P  Troncy E 《American journal of veterinary research》2007,68(3):297-304

OBJECTIVE: To compare glomerular filtration rate (GFR) as estimated from Patlak plot analysis by use of single-slice computed tomography (CT) with that obtained from clearance of plasma inulin in pigs. ANIMALS: 8 healthy anesthetized juvenile pigs. PROCEDURES: All pigs underwent precontrast, whole-kidney, helical CT; postcontrast single-slice dynamic CT; and postcontrast, whole-kidney CT for volume determination. On dynamic images, corrected Hounsfield unit values were determined for each kidney and the aorta. A Patlak plot for each kidney was generated, and plasma clearance per unit volume was multiplied by renal volume to obtain whole-animal contrast clearance. Mean GFR determined via inulin clearance (Inu-GFR) was measured from each kidney and correlated to mean GFR determined via CT (CT-GFR) for the left kidney, right kidney, and both kidneys by use of linear regression and Bland-Altman analyses. RESULTS: CT-GFR results from 7 pigs were valid. Total and right kidney Inu-GFR were correlated with total and right kidney CT-GFR (total, R(2) = 0.85; right kidney, R(2) = 0.86). However, left kidney CT-GFR was poorly correlated with left kidney Inu-GFR (R(2) = 0.47). Bland-Altman analysis revealed no significant bias between Inu-GFR and CT-GFR for the left kidney, right kidney, or both kidneys. CONCLUSIONS AND CLINICAL RELEVANCE: CT-GFR as determined by use of a single-slice acquisition technique, low-dose of iohexol, and Patlak plot analysis correlated without bias with Inu-GFR for the right kidney and both kidneys (combined). This technique has promise as an accurate CT-GFR method that can be combined with renal morphologic evaluation.  相似文献