Linseed oil in boar's diet improved in vivo fertility and antioxidant status     

Mahak Singh  Rongsennangba Talimoa Mollier  Renphamo Nzanthung Patton  Nungshitula Pongener  Lahar Jayoti Bordoloi  Rakesh Kumar  Rahul Katiyar  Meraj Haider Khan  Dipjayoti Rajkhowa  Vinay Kumar Mishra 《Reproduction in domestic animals》2023,58(1):27-38

The reactive oxygen species (ROS) which are produced during storage of boar semen are causing oxidative stress and leads to poor fertility. Also, tropical and sub-tropical weather condition adversely impacts the physicomorphological quality and fertility of boar sperm. The aim of this study was to examine the effects of feeding linseed oil to boar on its seminal attributes, sperm kinetics, biomarkers of antioxidant, fatty acid profile of seminal plasma (SP) and sperm and in vivo fertility. Six Hampshire crossbreed boars were fed with 90 ml linseed oil (LIN) whereas six Hampshire crossbreed boars were fed 90 ml canola oil (CON) for 16 weeks. Sperm quality was evaluated (60 ejaculates for each group; a total of 120 ejaculates) for motility, livability, abnormal morphology, acrosomal membrane integrity, hypo-osmotic swelling test (HOST) and sperm kinetic parameters by computer assisted semen analysis (CASA) at 0 h and at 72 h of storage at 17°C. Biomarkers of antioxidant (glutathione peroxidase; GPx, catalase; CAT, total antioxidant capacity; TAC) and malondialdehyde (MDA) were measured in SP and serum. Gas chromatography–mass spectrometry (GC–MS) was used for the estimation of fatty acid composition of SP and sperm. Boars fed with linseed oil had higher semen volume (p < .01) and more total sperm numbers (p < .01). Feeding linseed oil to boar enhanced seminal attributes (p < .05) at 0 h as well as at 72 h of storage. Linseed oil feeding (p < .01) improved biomarkers of antioxidants and significantly (p < .01) lowered the lipid peroxidation in serum and SP. Linseed oil feeding (p < .05) increased the proportion of alpha linolenic (ALA), arachidonic and docosahexaenoic (DHA) fatty acids in SP. The ratio of n-6 to n-3 fatty acids in sperm increased significantly (p < .01) in treatment group. Farrowing rate was significantly (p < .05) higher in treatment group. In conclusion, feeding linseed oil to boar improved the in vivo fertility, enhanced antioxidant capacity and increased the DHA content of SP and sperm.  相似文献   

Evaluation of dipstick-ELISA using 28 kDa Fasciola gigantica cathepsin l cysteine proteinase (FgCL3) for serodiagnosis of fasciolosis in naturally infected goats     

Gupta A  Dixit AK  Dixit P  Mahajan C  Sharma RL 《Veterinary parasitology》2011,176(2-3):165-169

In the present investigation 100 goats presented for slaughter were used to evaluate the 28kDa cathepsin l cysteine proteinase (FgCL3) dipstick-ELISA for the diagnosis of fasciolosis. Presence of Fasciola gigantica worms in liver at the time of slaughter was taken as a gold standard for the evaluation of the assay. Faeces, blood and liver were taken from all slaughtered goats. Biochemical parameters and coprological examinations using sedimentation technique were also taken into consideration. Total serum proteins, albumin, mean values of aspartate aminotransferase and alkaline phosphatase of positive group were not significantly different from those of the negative group. The diagnostic sensitivity of dipstick-ELISA was higher than coprological detection of fluke eggs. However, specificity was equal for both the tests. The accuracy of dipstick-ELISA in detecting circulatory antibodies during the course of disease was higher than coproscopic examination. In conclusion, the FgCL3 antigen dependent dipstick-ELISA was more reliable and has better scope for field application in the endemic areas.  相似文献   

Utstein‐style guidelines on uniform reporting of in‐hospital cardiopulmonary resuscitation in dogs and cats. A RECOVER statement          下载免费PDF全文

Manuel Boller Dr med vet  MTR  DACVECC  Dan J. Fletcher DVM  PhD  DACVECC  Benjamin M. Brainard VMD  DACVECC  DACVAA  Kate Hopper BVSc  PhD  DACVECC  Vinay M. Nadkarni MD  MS  FCCM  Peter T. Morley MBBS  FRACP  FANZCA  FCICM  FERC  Maureen McMichael DVM  DACVECC  Ryohei Nishimura DVM  PhD  Joris H. Robben DVM  PhD  DECVECC  Elizabeth Rozanski DVM  DACVECC  DACVIM  Elke Rudloff DVM  DACVECC  John Rush DVM  MS  DACVIM  DACVECC  Andre Shih DVM  DACVAA  DACVECC  Sean Smarick VMD  DACVECC  Luis H. Tello MV  MS  DVM 《Journal of Veterinary Emergency and Critical Care》2016,26(1):11-34

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Quality Assurance and Shelf-Life Extension of Kinnow Mandarin Fruit Under Supermarket Conditions     

B. V. C. Mahajan  Rupinder Singh  Mahesh Kumar 《International Journal of Fruit Science》2016,16(1):94-102

Kinnow mandarin (Citrus nobilis × Citrus deliciosa) fruits were harvested at firm mature stage, packed in paper-molded trays, and tightly sealed in different packaging films commercially available in the market, viz. cryovac heat shrinkable RD-106 film (15 µ), low density polyethylene film (LDPE, 25 µ), and high density polyethylene film (HDPE, 10 µ). After packaging, the fruits were stored at supermarket conditions (18 to 20 °C; 80–85% RH). There were five storage intervals and for each storage interval three packs (six fruit in each pack) were prepared for each packaging film. In total, 60 packs were made for all packaging films including control to lay out this experiment. The fruits were evaluated for various quality attributes periodically. The shrink film helped in reducing the loss in weight and firmness and maintained the various quality attributes, such as total soluble solids, acidity, ascorbic acid, and carotene content of the fruit juice, during shelf life better than other packaging films and unwrapped control fruits. The in-package gaseous composition (O₂ and CO₂) in shrink film packed fruits was found to be at a desired level, which resulted in maintaining pleasant flavor of the fruits. On the other hand, LDPE and HDPE film accumulated a very high level of CO₂, which led to formation of a fermenting odor in the package. The data revealed that RD-106 film proved quite effective in prolonging the shelf life and maintaining the quality of Kinnow fruits for 25 days under supermarket conditions as opposed to 10 days only in the case of unpacked control fruits.  相似文献   

Boundary layer halogens in coastal Antarctica     

Saiz-Lopez A  Mahajan AS  Salmon RA  Bauguitte SJ  Jones AE  Roscoe HK  Plane JM 《Science (New York, N.Y.)》2007,317(5836):348-351

Halogens influence the oxidizing capacity of Earth's troposphere, and iodine oxides form ultrafine aerosols, which may have an impact on climate. We report year-round measurements of boundary layer iodine oxide and bromine oxide at the near-coastal site of Halley Station, Antarctica. Surprisingly, both species are present throughout the sunlit period and exhibit similar seasonal cycles and concentrations. The springtime peak of iodine oxide (20 parts per trillion) is the highest concentration recorded anywhere in the atmosphere. These levels of halogens cause substantial ozone depletion, as well as the rapid oxidation of dimethyl sulfide and mercury in the Antarctic boundary layer.  相似文献   

Transgressive segregation for yield traits in Oryza sativa IR58025B X Oryza meridionalis Ng. Bc2F3population under irrigated and aerobic conditions     

Chejerla Mohan Kumar Varma  Patil Kalmeshwer Gouda  Surapaneni Saikumar  Vinay Shenoy  Halagappa Eshwarappa Shashidhar  Sarla Neelamraju 《Journal of Crop Science and Biotechnology》2012,15(3):231-238

Wild species of the genus Oryza are a good source of beneficial alleles for enhancing rice yield under normal and adverse conditions. BC₂F₃ population was derived from a cross between Oryza sativa IR58025B and Oryza meridionalis Ng. (2n = 24, AA) a heat tolerant wild species to evaluate 12 yield traits under irrigated and aerobic conditions. Analysis of variance and genetic estimates indicated there is substantial genetic variation among progenies under both conditions. Grain yield had high heritability (61.9%) and genetic advance (36.4%) under irrigated conditions but moderate heritability (49.6%) and genetic advance (13.3%) under aerobic conditions indicating that selection for yield will be effective under both conditions. Panicle number, grain number, spikelet fertility, and test weight showed significant positive correlation with grain yield under both conditions. Families out-performing IR58025B for yield under both conditions were obtained providing evidence that phenotypically inferior O. meridionalis contributed to yield increase. This species can be a novel source of natural genetic variation for the improvement of rice under irrigated as well as under aerobic condition.  相似文献   

Nutritional requirement of ascorbic acid by Indian major carp,Cirrhina mrigala,during early growth     

C.L. Mahajan  N.K. Agrawal 《Aquaculture (Amsterdam, Netherlands)》1980,19(1):37-48

Using synthetic diets, hatchlings of Cirrhina mrigala (obtained through induced breeding) were fed graded levels of ascorbic acid: 0, 60, 300, 600, 900, and 1200 mg/kg diet for 240 days. Studies on survival, growth and morphological changes of these fishes are reported. Statistical analysis of growth, deformity and survival rates at these levels suggested an optimum requirement of 650–750 mg ascorbic acid/kg diet in this early part of the life of the fish. Avitaminosis resulted in poor growth, high mortality rate (up to 42%), severe haemorrhages, fin necrosis, increased pigmentation and spinal flexures. 60 mg and 300 mg ascorbic acid/kg dietary levels also resulted in poor survival and growth rates. Skeletal deformities were also observed in some of these fish.  相似文献   

Biodiversity in the barnyard millet (<Emphasis Type="Italic">Echinochloa frumentacea</Emphasis> Link,Poaceae) germplasm in India     

Arun Gupta  Vinay Mahajan  Mukesh Kumar  H. S. Gupta 《Genetic Resources and Crop Evolution》2009,56(6):883-889

One hundred ninety four accessions of barnyard millet collected from different eco-geographical regions of India were evaluated for 14 quantitative traits during kharif 2003 and 2005. These accessions were grouped into 5 groups on the basis of place of collection. These groups differed significantly in their mean values for quantitative traits and magnitude of correlation among traits indicates origin based association. The group ‘C’ (source of origin is unknown) was found most diverse group (mean coefficient of variation 17.67%), while rest of the groups recorded mean coefficient of variation between 12 and 13%. Correlation studies indicate that flag leaf width, number of racemes along with internode length should be considered while performing selection in segregating generations. The plotting of first and second principal component axes scores suggests that presence of three distinct morphotypes in the present study. These morphotypes are quite similar to three botanical varieties viz., var. robusta, var. intermedia and var. stolonifera in their morphological traits, while botanical variety laxa (endemic to Sikkim area of India) was completely absent from the present study.  相似文献   

Effects of water temperature on mortality in Megalocytivirus‐infected rock bream Oplegnathus fasciatus (Temminck et Schlegel) and development of protective immunity          下载免费PDF全文

M H Jung  S J Jung  T N Vinay  C Nikapitiya  J O Kim  J H Lee  J Lee  M J Oh 《Journal of fish diseases》2015,38(8):729-737

Rock bream iridovirus (RBIV) causes huge losses, especially in rock bream Oplegnathus fasciatus. Rock bream injected with RBIV and held at 29, 26, 23 or 20 °C had 100% mortality. Conversely, all infected fish held at 17 °C survived even after the temperature was progressively increased to 26 °C at 100 dpi. Rock bream exposed to virus and held for 2, 4 and 7 days at 23/26 °C before the temperature was reduced to 17 °C had mortality rates of 26.6/73.2%, 66.6/100% and 93.4/100%, respectively, through 100 dpi. When surviving fish had the water temperature increased from 17 to 26 °C at 100 dpi, they did not exhibit signs of disease and had low virus copy numbers (below 10³). To investigate the development of a protective immune, rock bream were infected with RBIV and held at 23 °C before shifting the water temperature to 17 °C at 4 dpi. All injected fish survived until 120 dpi. While 100% of the previously unexposed fish died, 80.2% of the previously infected fish survived. When the survivors were rechallenged again at 160 dpi, no further mortality occurred. The high survival rate of fish following rechallenge with RBIV indicates that protective immunity was established in the surviving rock bream.  相似文献   

Isolation and characterization of lipopolysaccharides from different rhizobial isolates     

Mahipal Singh Kesawat  Basanta Kumar Das  G. R. Bhaganagare  Vinay Sharma  Manorama 《Journal of Crop Science and Biotechnology》2009,12(3):109-113

Formation of nodules on roots or in stems (in some cases) of leguminous plants is the unique ability of gram-negative bacteria, Rhizobia, which converts atmospheric nitrogen into usable forms by the host plant. Lipopolysaccharide (LPS) is the outer membrane component of the gram-negative bacteria, known to be an essential factor in host recognition, specificity, and initial infection processes. In the present study, we extracted lipopolysaccharides from different rhizobial isolates by a modified phenol-water method and partially characterized by polyacrylamide gel electrophoresis with silver staining. The results showed two separate banding regions, LPS-I and LPS-II. The high molecular weight and electrophoretic mobility of LPS-I region resembles that of lysozyme, used as a standard marker. The LPS-II region has a low molecular weight and electrophoretic mobility greater than that of lysozyme. The LPS-II region was due to incomplete LPS, which either lacks the entire O-antigen repeating unit or contains only one or two repeating units. The banding patterns of LPS vary among the different rhizobial isolates. Results revealed that the type of LPS structure and banding regions greatly facilitate the further characterization of the LPS modifications required for symbiosis.  相似文献