蔬菜农残快检假阳性关键因子及控制对策分析          下载免费PDF全文

张瑜  周钦方  王丽芳  王雯  曹丽芬  王斌 《农业工程》2023,13(5)

为进一步探究蔬菜农残快检假阳性问题,本文采用酶抑制率法和色谱法对大批量蔬菜样品中有机磷和氨基甲酸酯类农药残留情况进行研究分析,针对假阳性蔬菜特点,结合日常检测工作经验,对易引起酶抑制率快检假阳性的因素进行识别和分析,将关键影响因子分为酶抑制物干扰、竞争性显色反应干扰、底色干扰和透光干扰四大类。阐述了其对酶抑制反应的影响方式,并针对性地提出避免和减少假阳性情况发生的措施和建议,为今后酶抑制率快检方法改进、进一步提高快检准确率提供参考和依据。  相似文献   

膜下滴灌结合暗管排水技术改良新疆盐碱地效果综述          下载免费PDF全文

张万恒  何帅  忠智博  周建伟  郑国玉  马军勇 《农业工程》2023,13(4)

膜下滴灌技术的大面积推广和应用,为新疆乃至西北干旱地区作物高产提供了强大的技术支撑。应用在盐碱地改良中,膜下滴灌“有灌无排”模式仅调节耕层盐分分布,并不能将盐分排出土体,长年灌溉容易引起爆发式积盐的风险,而暗管排水是目前公认的改良盐碱地最直接、最有效的方法。目前,将膜下滴灌与暗管排水有机结合形成的新型灌溉技术,充分利用膜下滴灌良好的抑蒸增温、节水抑盐等优势,同时利用暗管将淋洗盐水及时排出土体,使盐碱化土层产生新的水盐平衡,已在新疆盐碱地区开发利用中得到长足发展。本文重点分析并总结了新疆灌区膜下滴灌结合暗管排水系统的设计参数及土壤水盐运移规律,并综述了土壤理化性质及作物产量对该灌排技术的响应研究,以期为新疆盐碱地农业可持续发展提供理论依据和技术指导。  相似文献   

新疆生产建设兵团葡萄种植布局综合评价研究          下载免费PDF全文

周丹丹  王民敬  韩萌  冯伟  赵威  赵毅  李健 《农业工程》2023,13(3)

葡萄是新疆生产建设兵团林果业的主导产业之一,兵团葡萄在新疆和全国占有重要地位。本研究在基于层次分析法的鲜食葡萄生态适宜性评价、综合比较优势指数的鲜食葡萄比较优势分析和成本收益的市场需求分析基础上,开展的兵团鲜食葡萄种植布局综合评价,研究结果表明：鲜食葡萄优良种植区域集中在天山北麓、伊犁河谷与吐哈盆地的农四师、农六师、农八师、农十三师等为鲜食葡萄重点生产地区,评价结果与生产实际总体相一致,为兵团鲜食葡萄种植布局优化提供可量化的参考依据。  相似文献   

Effects of soluble tea polysaccharides on hyperglycemia in alloxan-diabetic mice   总被引：2，自引：0，他引：2  

Zhou X  Wang D  Sun P  Bucheli P  Li L  Hou Y  Wang J 《Journal of agricultural and food chemistry》2007,55(14):5523-5528

The effects of tea water extracts (TWE), crude tea polysaccharides (CTP), and a tea polysaccharide fraction (TPF) were tested on hyperglycemic diabetic mice. Results indicated that TWE, CTP, and TPF could significantly decrease fasting blood glucose (FBG) and glucosylated serum protein (GSP) in alloxan-induced diabetic mice compared to the control group. In vitro antioxidant activities of TWE, CTP, and TPF for scavenging hydroxyl radicals and superoxide radicals decreased with the degree of purification and were lowest for TPF. High-performance gel permeation chromatography (HPGPC) and component analysis revealed the molecular mass distribution and constituents of TWE, CTP, and TPF, indicating that a 100-120 kDa fraction contained the hypoglycemic activity. This fraction was essentially composed of polysaccharides (approximately 90%) with substantial amounts of arabinogalactan proteins. The second-derivative IR spectra of TWE, CTP, and TPF with peak intensity around 1075 and 1045 cm(-1), which characterize galactopyranose in the backbone and arabinofuranose units in side branches, respectively, further substantiated the importance of the arabinogalactan proteins. Taken together, the results indicate that a soluble tea polysaccharide is the major hypoglycemic factor in tea and that this polysaccharide may be developed to a potential natural hypoglycemic functional ingredient.  相似文献   

Comparison of various extraction methods for policosanol from rice bran wax and establishment of chromatographic fingerprint of policosanol     

Wang MF  Lian HZ  Mao L  Zhou JP  Gong HJ  Qian BY  Fang Y  Li J 《Journal of agricultural and food chemistry》2007,55(14):5552-5558

A capillary gas chromatographic (GC) method has been developed for the separation and determination of policosanol components extracted from rice bran wax. A Varian CP-sil 8 CB column was employed, and an oven temperature was programmed. Gas chromatography-mass spectrometry (GC-MS) was used to identify the composition of policosanol. Quantitative analysis was carried out by means of hydrogen flame ionization detector (FID) with dinonyl phthalate (DNP) as internal standard. The results indicated that the extract obtained by dry saponification has the highest contents of octacosanol and triacontanol among extracts by all used extraction methods including dry saponification, saponification in alcohol, saponification in water (neutralized and non-neutralized), and transesterification. Meanwhile, the GC-MS fingerprint of policosanol extracted by dry saponification has been established. Euclidean distance similarity calculation showed remarkable consistency of compositions and contents among 12 batches of policosanol from a rice bran wax variety. This protocol provided a rapid and feasible method for quality control of policosanol products.  相似文献   

A systematic and quantitative approach to improve water use efficiency in agriculture   总被引：6，自引：2，他引：4  

Theodore C. Hsiao  Pasquale Steduto  Elias Fereres 《Irrigation Science》2007,25(3):209-231

As the competition for the finite water resources on earth increases due to growth in population and affluence, agriculture is faced with intensifying pressure to improve the efficiency of water used for food production. The causes for the relatively low water use efficiency in agriculture are numerous and complex, including environmental, biological, engineering, management, social, and economic facets. The complexity of the problem, with its myriads of local variations, requires a comprehensive conceptual framework of the underlying physical and biological processes as the basis to analyze the existing situation and quantify the efficiencies, and to plan and execute improvements. This paper proposes such a framework, based on the simple fact that the overall efficiency of any process consisting of a chain of sequential step is the product of the efficiency (i.e., output/input ratio) of its individual component steps. In most cases of water use, a number of process chains, both branching and merging, are involved. Means to integrate the diverging and converging chains are developed and presented as equations. Upscaling from fields to regions and beyond are discussed. This chain of efficiencies approach is general and can be applied to any process composed of chains of sequential steps. Here the framework is used to analyze the systems of irrigated and dryland crop production, and animal production on rangeland. Range of plausible efficiencies of each step is presented as tables, with values separately for the poor and for the good situation of circumstances, management and technology. Causes of the differences in efficiency of each step, going from water delivery to soil water extraction, transpiration, photosynthesis, and conversion to crop biomass and yield, and to animal product are briefly discussed. Sample calculations are made to demonstrate how modest differences in the efficiencies of the component steps are manifested as large to huge differences in the overall efficiency. Based on an equation quantifying the impact of changes in efficiency of component steps on the overall efficiency, it is concluded that generally, it is more effective to made modest improvements in several or more steps than to concentrate efforts to improve one or two steps. Hence, improvement efforts should be systematic and not overly concentrated on one or two components. The potential use of the same equation as the point of departure to optimize the allocation of economic resource among the component steps to maximize the improvement in the overall water use efficiency is elaborated on. The chain of efficiencies framework provides the means to examine the current levels of efficiency along the pathways of agricultural water use, to analyze where inefficiencies lie by comparing with the range of known efficiency values in the tables presented, to assess the potential improvements that may be achieved in various parts and their impact on the overall efficiency, and to aid in the optimal allocation of resources for improvements.

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Theodore C. HsiaoEmail:

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Determination of the crop coefficient for grafted ‘Tahiti’ lime trees and soil evaporation coefficient of Rhodic Kandiudalf clay soil in Sao Paulo,Brazil     

José AlvesJr  Marcos V. Folegatti  Larry R. Parsons  Wije Bandaranayake  Claudio R. da Silva  Tonny J. A. da Silva  Luís F. S. M. Campeche 《Irrigation Science》2007,25(4):419-428

The expansion of permanent trickle irrigation systems in Sao Paulo (Brazil) citrus has changed the focus of irrigation scheduling from determining irrigation timing to quantifying irrigation amounts. The water requirements of citrus orchards are difficult to estimate, since they are influenced by heterogeneous factors such as age, planting density and irrigation system. In this study, we estimated the water requirements of young ‘Tahiti’ lime orchards, considering the independent contributions from soil evaporation and crop transpiration by splitting the crop coefficient (Kc = ETc/ETo) into two separate coefficients; Ke, a soil evaporation coefficient and Kcb, a crop transpiration coefficient. Hence, the water requirement in young ‘Tahiti’ lime (ET_y) is ET_y = (Ke + Kcb) · ETo, where ETo is the reference crop evapotranspiration. Mature tree water requirement (ET_m) is ET_m = Kcb · ETo, assuming no soil water evaporation. Two lysimeters were used; one was 1.6 m in diameter and 0.7 m deep, and the other was 2.7 m in diameter and 0.8-m deep. The first one was used to calculate evaporation and the second one was used for transpiration. ETo was estimated by the Penman–Monteith method (FAO-56). The measurements were conducted during a period between August 2002 and April 2005 in Piracicaba, Sao Paulo state, Brazil. The lysimeters were installed at the center of a 1.0-ha plot planted with ‘Tahiti’ lime trees grafted on ‘Swingle’ citrumelo rootstock. The trees were 1-year old at planting, spaced 7 × 4 m, and were irrigated by a drip irrigation system. During the study period, Kc varied between 0.6 and 1.22, and Kcb varied between 0.4 and 1.0. The results suggested that for young lime trees, the volume of water per tree calculated by Ke + Kcb is about 80% higher than the volume calculated using Kc. For mature trees, the volume of water per tree calculated using just Kcb can be 10% less than using Kc. The independent influence of soil evaporation and transpiration is important to better understand the water consumption of young lime trees during growth compared to mature lime trees.  相似文献   

Effect of dietary daidzein on egg production, shell quality, and gene expression of ER-alpha, GH-R, and IGF-IR in shell glands of laying hens   总被引：1，自引：0，他引：1  

Ni Y  Zhu Q  Zhou Z  Grossmann R  Chen J  Zhao R 《Journal of agricultural and food chemistry》2007,55(17):6997-7001

Our previous studies demonstrated that dietary daidzein improves egg production in ducks during the late period of the laying cycle. The present study was aimed to investigate the effect of daidzein in laying hens, with more focus on eggshell quality. The expression of ER-alpha, GH-R, and IGF-IR mRNA in shell glands was determined to identify the target genes of daidzein action and to reveal the relationship between shell quality and profiles of gene expression in shell glands of laying hens. 1000 ISA hens, at 445 days of age, were allotted at random to two groups and given the basal diet with or without 10 mg of daidzein per kg diet for 9 weeks. Daidzein supplement significantly increased the egg laying rate and the feed conversion ratio. The eggshell thickness increased, while the percentage of cracked eggs decreased in daidzein-treated hens. Serum E2 and phosphate concentrations were not altered, but the level of serum Ca2+ and the tibia bone mineral density were significantly increased in the daidzein-treated group compared with their control counterparts. In parallel with the significant increase of oviduct weight, significant down-regulation of GH-R and IGF-IR mRNA and a trend of decrease in ERalpha mRNA expression in shell glands were observed in daidzein-treated hens. The results indicate that dietary daidzein improves egg laying performance and eggshell quality during the late (postpeak) laying stage of hens, which is associated with modulations in gene expression in the shell gland.  相似文献   

黄土半干旱区油松苗木蒸腾特性与影响因子的关系   总被引：4，自引：0，他引：4  

张卫强  贺康宁  朱艳艳  周毅  巩玉霞  唐道锋 《中国水土保持科学》2007,5(1):49-54

 为分析土壤水分与气象因子对油松蒸腾作用的影响程度,提高造林成活率,并为林地水分管理提供科学依据。在2004年生长季典型晴天,采用盆栽试验,人为控制土壤水分,利用针叶Li-1600稳态气孔仪和BP-3400精密天平等仪器,对黄土半干旱区油松苗木的蒸腾特性及其影响因子的关系进行研究。结果表明:在不同的土壤水分条件下,油松蒸腾速率和气孔阻力的日变化曲线分别呈“双峰型”和“W”型;在典型晴天下,蒸腾速率与土壤含水量的关系呈三次曲线相关,7、8和10月份,油松叶片蒸腾速率达到最大值时所对应的土壤含水量分别为17.7%、19.8%和17.5%。蒸腾速率除自身生理特性的影响外,还受土壤水分和气象因子综合影响,当土壤水分充足时,蒸腾速率与气象因子相关性高;当土壤水分产生胁迫时,蒸腾速率与气象因子相关性降低。在严重土壤水分胁迫下,7和8月份气温对蒸腾作用的影响最大,10月份光合有效辐射的影响最大。在土壤充分供水的条件下,7月份空气相对湿度对蒸腾作用的影响最大,8月份是气温,10月份是叶温。  相似文献   

An enrichment microsphere immunoassay for the detection of <Emphasis Type="Italic">Pectobacterium atrosepticum</Emphasis> and <Emphasis Type="Italic">Dickeya dianthicola</Emphasis> in potato tuber extracts     

Jeroen Peters  Wojciech Sledz  Jan H. W. Bergervoet  Jan M. van der Wolf 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(2):97-107

An enrichment microsphere immunoassay (MIA) was developed, based on the Luminex xMAP® technology, for the simultaneous (duplex) detection of Pectobacterium atrosepticum (former name Erwinia carotovora subsp. atroseptica) (Pca) and Dickeya dianthicola (former name Erwinia chrysanthemi) (Dcd) in potato plant extracts. Target bacteria in the extracts were enriched for 48 h in a semi-selective broth containing polypectate under low oxygen conditions. Samples were subsequently incubated with antibody-coated colour-coded microspheres (beads) and with secondary antibodies conjugated with Alexa Fluor® 532, a reporter dye. Samples were analyzed with the Luminex analyzer, in which one laser identified each microsphere and another laser the reporter dye conjugated to the secondary antibodies. The assay required minimal sample preparation, could be completed in 1 h, was performed in 96 wells microtitreplates and required no wash steps. The limit of detection for the duplex enrichment MIA was 100–1000 cfu ml^?1, which was a hundred times lower than of an enrichment-ELISA. Without enrichment, the sensitivity of MIA and ELISA was largely similar and ranged between 10⁶ and 10⁷ cells ml^?1. No difference in sensitivity was found between a MIA in a single or duplex format. In a comparative test with non-infected potato plant extracts and extracts from plants infected with Pca or Dcd, results of the enrichment MIA correlated well with those of the enrichment ELISA and enrichment PCR. These results indicate that MIA can be reliably used for multiplex detection of soft rot Enterbacteriaceae in crude potato plant extracts. The technology is an attractive and cost-effective alternative to other detection methods, including ELISA.  相似文献