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71.
Shin T Kim S Ahn M Kim H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):71-74
The expression of protein kinase C (PKC) was studied in the bovine retina by immunohistochemical analysis. Western blot analysis showed that PKC isoforms, including alpha, betaI, delta and theta, were detected in the bovine retina. By immunohistochemistry, both PKC alpha and betaI were expressed in all retinal layers, with an intense localization of both PKC alpha and betaI detected in bipolar cells in the inner nuclear cell layer and in some glial cells in ganglion cell layers. The immunoreactivity of both PKC delta and theta was quite weak in the retinal layers, compared with that of PKC alpha and betaI. These findings suggest that both conventional and novel PKCs are differentially expressed in the bovine retina. 相似文献
72.
Production of blastocysts after intergeneric nuclear transfer of goral (Naemorhedus goral) somatic cells into bovine oocytes 总被引:1,自引:0,他引:1
Oh BC Kim JT Shin NS Kwon SW Kang SK Lee BC Hwang WS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1167-1171
Interspecies cloning may be a useful method to help conserve endangered species and to study nuclear-cytoplasm interaction. The present study investigated in vitro development of goral (Naemorhedus goral) intergeneric nuclear transfer embryos produced by fusing goral fibroblasts with enucleated metaphase II (MII) bovine oocytes. After two to five passages, serum-starved or non-starved goral skin fibroblast cells were transferred into enucleated MII bovine oocytes. Couplets were electrically fused and chemically activated, and then cultured in either modified synthetic oviduct fluid (mSOF) or tissue culture medium-199 (TCM-199) supplemented with 10% FBS. Serum starvation of donor cells did not affect the fusion rate and or development to of cells to the two-cell stage, to more than 9-cells, or to morulae, regardless of culture medium. Three blastocysts from 202 fused embryos were obtained when embryos reconstructed with non- serum- starved donor cells were cultured in mSOF. However, no blastocysts were obtained when the embryos reconstructed with serum-starved donor cells were cultured in mSOF. The total cell number of goral intergeneric embryos averaged 130.3 (range 105-180). In conclusion, this study demonstrated that bovine oocytes can support blastocyst development after intergeneric SCNT with goral fibroblasts. 相似文献
73.
Moon C Jeong CW Kim H Ahn M Kim S Shin T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(7):761-764
The expression pattern of CD44 was studied in the rat testis following ischemia/reperfusion (I/R) injury to elucidate the possible role of the CD44 adhesion molecule in acute experimental testicular torsion. Western blot analysis showed that CD44 expression began to increase significantly 24 hr after reperfusion, compared with the normal control; the increased expression persisted until 96 hr after I/R. Immunohistochemistry showed that, in the normal testis, CD44 was constitutively expressed mainly in ED2-positive resident macrophages in the interstitial space. After I/R, the majority of inflammatory cells in the interstitial space surrounding the damaged tubules were ED1-positive macrophages that were CD44-positive. These findings suggest that the significant increase in CD44 expression that occurs during the delayed phase after reperfusion originates from infiltrating macrophages possibly in anticipation of the migration and adhesion of additional macrophages into the affected testis. 相似文献
74.
Nakamura K Murata C Ito M Iwamori T Nishimura S Hisamatsu S Sonoki S Nakayama A Suyama E Kawasaki H Taira K Nishino K Tachi C 《The Journal of reproduction and development》2006,52(1):73-80
As the first step in investigating the possiblity of applying ribozyme technology to artificial control of the sex ratios at birth in farm animals, where the demand for females exceeds that for males, we designed a hammerhead ribozyme (HHRz) and 2 tRNA(val)-hammerhead ribozyme complexes (tRNARz3 and tRNARz4), and examined their effects upon murine Sry mRNA in vitro and in cells. We demonstrated that HHRz and tRNARz3 could effectively cleave the target Sry mRNA in vitro. For the purpose of experiments in vivo, HHRz was cloned into the highly efficient pUC-CAGGS mammalian expression vector (pCAG/HHRz), and the tRNA ribozyme complexes were cloned into the pol III promoter-driven pPUR-KE vector (pPUR/tRNARz3 and pPUR/tRNARz4); the ribozyme vectors were co-transfected with the target vector (pCAG/Sry). A suppressive action (up to approx. 60%) was confirmed for pCAG/HHRz and pPUR/tRNARz3 upon the transiently expressed exogenously introduced Sry in M15 cultured cells. 相似文献
75.
Lee JH Lee KT Akoh CC Chung SK Kim MR 《Journal of agricultural and food chemistry》2006,54(15):5416-5421
Structured lipid (SL) was synthesized from extravirgin olive oil (EVOO) and conjugated linoleic acid (CLA) via a lipase-catalyzed reaction. CLA provides a variety of health benefits, but it is not consumed in free fatty acid form. The synthesized SL olive oil contained 42.5 mol % CLA isomers, and the major isomers were cis-9,trans-11-CLA (16.9 mol %) and trans-10,cis-12-CLA (24.2 mol %). The antioxidant activity determined by the radical scavenging capacity with the 2,2-diphenyl-1-picrylhydrazyl radical was lower in SL olive oil than in EVOO. The oxidative stability was also lower in SL olive oil since it had a higher peroxide value, rho-anisidine value, and 2-thiobarbituric acid reactive substances values during 20 days of storage at 60 degrees C. This observation could be due to the reduction in the natural phenolic compounds (97%) and tocopherols (56%), and the incorporated CLA with two conjugated double bonds in the SL olive oil. The oxidative stability of SL olive oil was increased by added rosemary extracts at concentrations of 100, 200, and 300 ppm. The present study suggests that the SL olive oil may be a suitable way to incorporate or deliver CLA into human diets. However, the addition of a proper antioxidant would be required for improving its oxidative stability. 相似文献
76.
Sang-Kuk Kim Shin-Young Park Kye-Ryung Kim Jong-Hee Shin Su-Yong Kim Hak-Yoon Kim In-Jung Lee 《Journal of Crop Science and Biotechnology》2012,15(4):305-310
This study was carried out to determine the pretreatment effect of proton beam irradiation on germination and pasting properties of starches in two rices. Mature and healthy seeds irradiated with 10 doses (0, 50, 100, 200, 300, 400, 500, 600, 700, and 800 Gy) for determination of LD50 and characteristics of germination were recorded at 14 days after irradiation. The rice seeds irradiated with five doses (0, 50, 100, 200, and 300 Gy) were used to evaluate the irradiation effects of pasting properties of starches. It showed that a lower survival rate in germinated rice seeds was observed above 300 Gy showing 31 and 35% in Ilpum and Hanmaum, respectively. The higher plant height and root length were also recorded in 50 and 100 Gy. Amylose content in proton beam irradiated with 50, 100, 200, and 300 Gy was significantly decreased in two rice cultivars. Peak viscosity, hot peak viscosity, cooling peak viscosity, and setback viscosity decreased with increasing proton beam dose levels. The degree of crystallinity was significantly increased with increasing proton beam dose levels. Consequently, it might be deduced that proton beam irradiation causes changes of starch properties affecting crystalline regions of starch granules, especially at high dosage irradiation. 相似文献
77.
Kametani Y Ohshima S Kita YF Shimada S Kamiguchi H Shiina T Inoko H Kulski JK Ando A 《Veterinary immunology and immunopathology》2012,148(3-4):252-259
The expression of the major histocompatibility complex (MHC) classical class I genes is important for the adaptive immune response to target virus-infected cells and cancer cells. The up-regulation of the MHC is achieved by hormonal/cytokine signals including IFN-γ-inducible elements. The swine leukocyte antigen (SLA), the MHC class I region of pigs, consists of the duplicated classical class I genes, SLA-1, SLA-2 and SLA-3, but the molecular mechanisms involved in their up-regulation after T cell stimulation have not been fully elucidated. In order to better understand some of the putative regulatory mechanisms of SLA class I gene expression in activated T cells, we examined the coordinated expression of the SLA classical class I, IFN-γ and interferon regulatory factor-1 (IRF-1) genes in the peripheral blood mononuclear cells (PBMCs) of SLA homozygous Clawn miniature swine stimulated for 72h with either IFN-γ or an enterotoxin produced by Staphylococcus aureus. This enterotoxin, toxic shock syndrome-1 (TSST-1), is known to act as a superantigen (sAG) to activate the T cells in various vertebrate species. We showed by using mAbs and flow cytometry that the CD4(+)CD25(+) cell number of swine PBMCs was also increased by TSST-1 and to a lesser degree by IFN-γ. Time course analyses of the expression of the IFN-γ, IRF-1 and the three classical class I genes, SLA-1, SLA-2, and SLA-3, in PBMCs by quantitative real-time PCR revealed a transitory response to TSST-1 or IFN-γ stimulation. The IFN-γ mRNA levels in the PBMCs were continuously up-regulated over the first 48h by TSST-1 or IFN-γ. In contrast, SLA class I expression moderately increased at 24h and then decreased to a baseline level or less at 72h of IFN-γ or TSST-1 stimulation. The three classical SLA class I genes showed similar expression kinetics, although SLA-3 mRNA level was consistently lower than those of SLA-1 and -2. The expression of IRF-1, a modulator of SLA expression, showed similar kinetics to those of the three classical SLA class I genes. The expression profiles detected by flow cytometry of the SLA molecules on the cell surface of PBMCs were maintained at a consistently high level during cell stimulation with either TSST-1 or IFN-γ, which was distinct from the kinetics of mRNA expression. These results showed that miniature swine SLA class I mRNA expression was effectively and equally up-regulated among the three loci and coordinately with IRF-1 gene expression after stimulation of T cell activation by sAG or IFN-γ. 相似文献
78.
Jeoung HY Shin BH Lee WH Song DS Choi YK Jeong W Song JY An DJ 《Journal of Feline Medicine and Surgery》2012,14(10):746-750
To investigate the potential transmission of subtype H3 influenza virus to cats, a serological survey was carried out in South Korea. Serum samples (n = 1027) were obtained from 809 pet cats and 218 domesticated cats living in urban colonies (D-cats) from 2008 to 2010, and tested using an influenza anti-nucleoprotein (NP)-specific enzyme-linked immunosorbent assay (ELISA) and the haemagglutination inhibition (HI) test, which was recommended by the World Organization for Animal Health. Anti-influenza virus antibodies were detected in 3.12% and 2.43% of cat sera tested using the NP-specific ELISA and HI test, respectively. Anti-H3 antibodies were also identified when the HI assay was used for influenza virus serotyping. These data may indicate the sporadic transmission of subtype H3 influenza virus from other infected species to cats in South Korea. 相似文献
79.
Yoshinobu UEMOTO Nanae SASAGO Tsuyoshi ABE Hideki OKADA Hitomi MARUOKA Hiroaki NAKAJIMA Noriaki SHOJI Shin MARUYAMA Naohiko KOBAYASHI Hideyuki MANNEN Eiji KOBAYASHI 《Animal Science Journal》2012,83(11):719-726
Genome‐wide association mapping for complex traits in cattle populations is a powerful, but expensive, selection tool. The DNA pooling technique can potentially reduce the cost of genome‐wide association studies. However, in DNA pooling design, the additional variance generated by pooling‐specific errors must be taken into account. Therefore, this study aimed to investigate factors such as: (i) the accuracy of allele frequency estimation; (ii) the magnitude of errors in pooling construction and in the array; and (iii) the effect of the number of replicate arrays on P‐values estimated by a genome‐wide association study. Results showed that the Illumina correction method is the most effective method to correct the allele frequency estimation; pooling errors, especially array variance, should be taken into account in DNA pooling design; and the risk of a type I error can be reduced by using at least two replicate arrays. These results indicate the practical capability and cost‐effectiveness of pool‐based genome‐wide association studies using the BovineSNP50 array in a cattle population. 相似文献
80.
Lichao Wu Yanlin Deng Shin Watanabe Shinzato Takakazu Jie Qin Jie Zhong Jin Zhang 《New Forests》2013,44(3):427-442
Natural regeneration by strip-clearcut has rarely been used for restocking of evergreen broad-leaved forests in the East Asia. To contribute to a better understanding of the likely reasons for either the successful recovery of these forests or failure, the early response of stand structure and tree species diversity to strip-clearcut were studied in Okinawa Island, southwest Japan. The 4,000 m2 study area was divided into 10 adjacent sections of equal area with a 10 m × 10 m sampling plot in the center, of which five sections were strip-clearcut while the remaining five sections uncut. Eight-year secondary succession following strip-clearcut showed that the density of woody stems, tree species diversity increased significantly not only in the regenerating stands but also in the residual stands compared to the primary stands. The primary dominant target species, Castanopsis sieboldii, continued to dominate both the regenerating and residual stands although colonizing species invaded abundantly in the stands, occupying approximately one-third of total tree species in each treatment. The community similarity analyses showed that the species composition were similar between the primary stands and the regenerating stands or residual stands reflected the high similarity amongst the primary stands and the regenerating stands or the residual stands. The similar stand structure, high tree species diversity and greater community similarity after strip-clearcut provided no evidence of forest degeneration, suggesting that the regenerating stand might gradually develop into stand similar to that prior to strip-clearcut. 相似文献