现代新型农业种植模式与推广          下载免费PDF全文

董兰阁 《农业工程》2019,9(6):142-145

随着社会经济的快速发展,我国农业进入转型升级的关键时期,面临着更多的机遇和挑战,唯有加快推动农业发展方式的转变,提升农业质量、效益和竞争力,才能夯实农业发展的根基,确保农民收入的稳定增加。通过对我国传统农业向现代化新型农业转型过程中存在问题的深入分析,探索适合我国国情的现代新型农业模式和农业科技服务体系的构建,并在此基础上预测现代新型农业的发展趋势和推广策略,坚持走优质、高效和绿色的农业生态道路,推动农业的可持续发展。  相似文献   

FELV infection, the kiss of death     

de Lange K 《Tijdschrift voor diergeneeskunde》2008,133(4):149

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FIV: not a death sentence     

de Lange K 《Tijdschrift voor diergeneeskunde》2008,133(10):436

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Characterization of ileal bacterial microbiota in newly-weaned pigs in response to feeding lincomycin, organic acids or herbal extract   总被引：2，自引：1，他引：1  

Joshua Gong  Hai Yu  Ting Liu  Mei Li  Weiduo Si  Cornelis F.M. de Lange  Cate Dewey 《Livestock Science》2008,116(1-3):318-322

The changes of ileal bacterial microbiota in pigs during the first 2 weeks post-weaning in response to feeding lincomycin, organic acids or herbal extract were characterized using 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis (DGGE) profiling, DNA sequencing, and real-time PCR (QPCR) techniques. Both time post-weaning and the dietary treatments resulted in a shift of the microbiota composition. While time post-weaning mostly influenced Clostridium group, the feed additives increased the population of Lactobacillus and related lactic acid bacteria by ≥ 3-fold.  相似文献   

Measures for interoperability of phenotypic data: minimum information requirements and formatting     

Hanna??wiek-Kupczyńska  Thomas?Altmann  Daniel?Arend  Elizabeth?Arnaud  Dijun?Chen  Guillaume?Cornut  Fabio?Fiorani  Wojciech?Frohmberg  Astrid?Junker  Christian?Klukas  Matthias?Lange  Cezary?Mazurek  Anahita?Nafissi  Pascal?Neveu  Jan?van?Oeveren  Cyril?Pommier  Hendrik?Poorter  Philippe?Rocca-Serra  Susanna-Assunta?Sansone  Uwe?Scholz  Marco?van?Schriek  ümit?Seren  Bj?rn?Usadel  Stephan?Weise  Paul?Kersey  Pawe??KrajewskiEmail author 《Plant methods》2016,12(1):44

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Persistence of viral RNA in rabbits which overcome an experimental RHDV infection detected by a highly sensitive multiplex real-time RT-PCR     

Gall A  Hoffmann B  Teifke JP  Lange B  Schirrmeier H 《Veterinary microbiology》2007,120(1-2):17-32

An internally controlled multiplex real-time RT-PCR using TaqMan probes and external standards for absolute RNA quantification was developed as a new diagnostic tool for the detection of rabbit haemorrhagic disease virus (RHDV). The test revealed a specificity of 100%, an analytical sensitivity of 10 copies/well and a linearity over a range from 10(1) to 10(10) copies. The viral loads in organs, leukocytes, sera and excretions of seropositive, convalescent rabbits which were overcoming an experimental infection with RHDV were determined using the validated assay. As a result, viral RNA was demonstrated and quantified for at least 15 weeks. Thus, a persistence of viral RNA after experimental infection of rabbits could be shown for the first time. In contrast, neither antigen nor infectious virus could be detected by antigen-ELISA, immunohistochemistry or experimental transmission. Therefore, further experiments are necessary to prove that the persistence of RNA is linked with the persistence of infectious virus particles.  相似文献   

The influence of fertilization and rotation on soil organic matter and plant yields in the long‐term Eternal Rye trial in Halle (Saale), Germany     

Lothar Schmidt  Kristina Warnstorff  Hannelore Drfel  Peter Leinweber  Henning Lange  Wolfgang Merbach 《植物养料与土壤学杂志》2000,163(6):639-648

Six forms of fertilizers and three rotations have been examined for 120 years in the ”︁Eternal Rye trial” in Halle (central German arid region, Haplic Phaeozem on sandy loess). Rank analysis, trend analysis and a new component model have been found to be suitable statistical methods to evaluate long‐term results without true replications. In this trial it was shown that mineral fertilization maintains like farmyard manuring the yield potential although at lower content of soil organic matter (SOM). Without fertilization, yield decline was greater with potato and silage maize than winter rye (Secale cereale L.). Deficiencies in N fertilization lead immediately, and in PK supply after more than 20 years, to a significant yield decrease which could be quickly compensated by full fertilization. Rye and maize monocultures also resulted in yield decreases. The establishing of new steady states in the turnover of SOM took about 50 years after changes in fertilization. Contrary to rye monoculture, both silage maize monoculture and potato alternating with winter rye caused considerable decomposition of SOM. According to analytical pyrolysis (Py‐FIMS and Py‐GC/MS), fertilization affects the SOM composition more than rotation. Without fertilization, a higher percentage of thermically stable SOM remained in comparison to the FYM soils. The introduction of potato into the rotation enhanced the content of easily decomposable SOM.  相似文献   

Towards an objective evaluation of persistency of <Emphasis Type="Italic">Lolium perenne</Emphasis> swards using UAV imagery     

Irene Borra-Serrano  Tom De Swaef  Jonas Aper  An Ghesquiere  Koen Mertens  David Nuyttens  Wouter Saeys  Ben Somers  Jürgen Vangeyte  Isabel Roldán-Ruiz  Peter Lootens 《Euphytica》2018,214(8):142

Perennial ryegrass (Lolium perenne) is a perennial crop used in temperate regions as forage. In L. perenne breeding programs, persistency is an important trait. Poor persistency results in sward degradation and associated yield and nutritive value losses. Breeders assess persistency of accessions using visual scoring in field plots during the 2nd or 3rd growing season. This evaluation system is easy and cheap but is not free from human bias. In this study, the correlation between the scoring done by different breeders was only 0.243. As an alternative we have developed a methodology to assess persistency of L. perenne breeding materials based on vegetation indices (VIs) derived from Unmanned Aerial Vehicle (UAV) imagery. The VIs Excess green (ExG2), Green Leaf Index and Normalized green intensity (GCC) were found to provide consistent results for flights carried out under different light conditions and were validated by ground reference information. The correlation between the VIs and the percentage of ground cover extracted from on-ground imagery was 0.885. To test the implementation of the method we compared the ExG2 value based approach to selection with a visual score based selection methodology as applied by two breeders. The breeding decisions of Breeder A agreed well with decisions based on ExG2 values (74.6%), but those of Breeder B displayed a lower agreement (54.0%). In contrast, agreement between decisions based on different flights was very high (91.6%). The methodology was validated for general applicability. In summary, the results demonstrate that basing persistency selection in L. perenne breeding programs on ExG2 values from UAV imagery is likely to be more objective in comparison to the currently-used visual scoring method.  相似文献   

Phenotypical parameters as a tool to evaluate the immunostimulatory effects of laminarin in Oncorhynchus mykiss          下载免费PDF全文

Byron Morales‐Lange  Jörn Bethke  Paulina Schmitt  Luis Mercado 《Aquaculture Research》2015,46(11):2707-2715

Laminarin is a β‐glucan from the brown algae Laminaria digitata (J.V. Lamour), which can activate innate immune responses. With the aim of developing a strategy to evaluate specific phenotypical parameters of the effects of laminarin in trout innate immunity, we (i) fed fish with laminarin‐supplemented diet (0.2 g kg^?1 day^?1) for 21 days and (ii) treated fish with a single dose of intraperitoneal injected laminarin (0.08 g kg^?1 fish). The evaluation of cellular and humoral immune parameters was established at phenotypic level by the phagocytic activity of headkidney macrophages and detection of inflammatory cytokines in head kidney and gill tissue by indirect ELISA. Results showed that both delivery methods of laminarin produce an increase in the phagocytic activity in head kidney macrophages and a significant increase in the production of TNFα and IL‐8 in gill tissue at day 21. Additionally, some of these parameters were significantly correlated (P < 0.025), which places them as new potential combined markers to detect activation of trout defense mechanisms by laminarin. These results highlight the importance of developing new protocols to quantitate immune parameters, in order to evaluate immunostimulants in fish farming.  相似文献   

Leptosphaeria spp., phoma stem canker and potential spread of L. maculans on oilseed rape crops in China     

X. Zhang  R. P. White  E. Demir  M. Jedryczka  R. M. Lange  M. Islam  Z. Q. Li  Y. J. Huang  A. M. Hall  G. Zhou  Z. Wang  X. Cai  P. Skelsey  B. D. L. Fitt 《Plant pathology》2014,63(3):598-612

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