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41.
AIM:To investigate the effects of fibronectin (FN) on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts (CFb) derived from SHR (CFbSHR) and WKY (CFbWKY). METHODS:CFb derived from 12-week-old spontaneously hypertensive rat (SHR) and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was measured by cell number counting and[3H]-TdR incorporation using 24-well plates pre-coated with 5 μg/cm2 of FN. Collagen synthesis was determined by [3H]-proline incorporation. RESULTS:As compared with control, the cell number of fibroblasts derived from SHR and WKY were significantly increased to 163.75% and 170.42% respectively after 72 h incubation with FN in the presence of 0.4% FCS from a intial cell density of 1×104 cells/mL. DNA synthesis of CFb was markedly promoted by FN. FN induced an increased in [3H]-proline incorporation in both CFbSHR and CFbWKY. CONCLUSION:FN is able to promote cell proliferation and collagen synthesis of CFb derived both from SHR and WKY. 相似文献
42.
孔雀河流域绿洲生态支持系统调控模式研究 总被引:8,自引:0,他引:8
生态环境用水量的减少是孔雀河流域近 50年来现代绿洲环境退化的主要原因 ,维持生态与社会经济的合理用水比例是绿洲系统持续发展的前提。以此为基础 ,本文采用水资源承载力的方法探讨了绿洲生态支持子系统和社会经济子系统优化调控模式 ,最后就配置方案及发展模式的可行性进行了分析 相似文献
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梨果实若干性状的遗传倾向 总被引:5,自引:0,他引:5
以梨12 个杂交组合和138 个栽培品种为试材, 研究几个果实性状的遗传倾向, 结果如下: 栽培品种果实含糖量集中在6 %~9 % , 平均为7. 39 %; 果实含糖量表现为数量性状, 呈正态分布, 杂种后代果实含糖量主要分布在6 %~10 % , 平均为7. 8 % , 遗传传递力平均为90. 8 %。栽培品种含酸量介于0. 08 %~0. 74 % , 平均为0. 238 %; 杂种后代果实含酸量为数量性状, 组合遗传传递力平均为69. 2 %。梨果实挥发性物质共检测出5 种酯类、8 种醇类和乙烯、乙醛、丙酮类共16 种化合物。杂种后代果实中发现了其亲本果实中所没有的戊醇成分。果实肉质性状表现为质量性状, 脆肉对软肉为显性。杂种后代表现为果点变大、密, 果柄变长, 果汁变少, 果皮厚度相当于亲中值。 相似文献
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AIM:To investigate the effect of the heat shock response on the reperfusion arrhythmias(RAs) and the possible mechanism involved. METHODS:Fifty-five Sprague Dawley rats were randomly divided into 2 groups: the heat shock group (group H,n=29) and the control group (group C,n=26). The rats in group H were preconditioned with heat shock 24 hours before, and that in group C were not. The hearts of 16 rats in group H and 16 in group C were exercised and mounted on a non-circulating Langendorff perfusion apparatus and perfused retrogradely with modified K-H buffer and mimic ischemia/reperfusion was applied. Additionally, conventional intracellular microelectrode techniques were used for recording such electrophysiological parameters as resting potential(RP), action potential amplitude(APA), over shot(OS), maximum depolarization velocity(Vmax) of the hearts of other 13 rats in group H and 10 in group C. RESULTS:①Prior heat stress significantly decreased reperfusion arrhythmia. ②The amount of CK release in the effluent in group H was much less than that in group C. ③Myocardial HSP70 content was elevated significantly in group H. ④Heat stress significantly increased myocardial anti-oxydases activity and decreased lipid peroxydative products. Additionally, heat stress significantly reduced the Vmax of action potential. It indicated that rapid Na+ channel of papillary muscles may be inhibited by heat shock. The degree of change of Vmax after ischemia in H group was significantly less than that in group C. And the time of reperfusoin with Tyrode's solution till the action potential appeared as large as that before perfusion with mimic ischemic solution is shorter in group H than in group C. CONCLUSION:Heat shock pretreatment markedly reduces ischemia/reperfusion-induced injury of heart and ventricular arrhythmias in rats and this effect may be associated with the inhibition of rapid Na+ channel of papillary muscles by heat shock and the increase in myocardial HSP70 and anti-oxydase activity. 相似文献
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CHEN Xiao-yin ZHANG Qun YANG Qin-he XIE Qiu-ling SHEN Qiang XU Yun-sheng 《园艺学报》2002,18(12):1529-1531
AIM: To examine the effect of traditional chinese medicine recipe, Taoren Honghua(semen persicae-flos carthami) decoction, on hyperlipidemia without symptom. METHODS: The plasma TC, TG, LDL, HDL, apolipoprotein(Apo) A, Apo Bof the patients with hyperlipidemia without symptom were measured using automatic analyzer (shimadiu CL-7200), the production of nitric oxide(NO) was detected by Greiss reaction, and SODactivity and MDAformation were examined using o-trihydroxy benzene and barbituric methotheds, respectively. RESULTS: After oral administration of Taoren Honghua decoction, the plasma levels of TC, TG, LDL, and MDAof the patients were markedly decreased, however, the plasma levels of ApoA, HDL, SODand NOwere significantly increased and almost no change was detected in the plasma level of Apo B. In control group, it was found that although the plasma level of TC, TG and LDL were decreased ( P<0.05 ) and ApoAas well as HDLwere increased, ApoB, SOD, MDA and NO production were all unchanged. CONCLUSION: The traditional chinese medicine recipe, Taoren Honghua decoction,has a significant therapeutic effect on patients with hyperlipidemia by removing blood stasis, promoting qi circulation and in turn reducing blood lipid level. 相似文献
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