ROCK promotes high glucose-induced cardiomyocyte apoptosis by inhi-biting PI3K/Akt signaling pathway     

BAI Xue  YU Hui  ZHU Ju-ru  HAN Zhong-li  YANG Xiao-min 《园艺学报》2020,36(5):788-795

AIMTo investigate whether Rho-associated coiled-coil kinase (ROCK) is involved in high glucose-induced apoptosis of primary cardiomyocytes by regulating PI3K/Akt signaling pathway. METHODSPrimary Wistar rat cardiomyocytes were cultured and identified by α-sarcomeric actin (α-SCA) immunohistochemistry. Cardiomyocytes were treated with 5.5, 33 and 40 mmol/L glucose for 48 h. The cell viability was measured by MTT assay, and the mRNA expression of ROCK1 and ROCK2 in the cardiomyocytes was detected by RT-qPCR. Flow cytometry was used to analyze the apoptosis of the cardiomyocytes. The protein levels of ROCK1, ROCK2, cleaved caspase-3, Bcl-2, PI3K, Akt and p-Akt were determined by Western blot. In order to confirm the regulatory effect of ROCKs on PI3K/Akt signaling pathway, the cells were divided into control group (5.5 mmol/L glucose), high glucose group (33 mmol/L glucose) and high glucose+Y27632 (ROCK inhibitor) group. Western blot was used to detect the protein levels of ROCK1, ROCK2, PI3K, Akt and p-Akt. RESULTSAfter 48 h of high glucose exposure, the values of relative cell viability in 33 and 40 mmol/L glucose groups were (79.71±2.43)% and (68.41±7.49)%, respectively, both of which were significantly decreased compared with normal control group (P<0.05). After 48 h of high glucose exposure, the relative mRNA levels of ROCK1 and ROCK2 in 33 and 40 mmol/L glucose groups were significantly increased compared with normal control group (P<0.05). Compared with normal control group, the apoptotic rate in 33 and 40 mmol/L glucose groups was increased significantly (P<0.05). Compared with normal control group, the protein expression of ROCK1, ROCK2 and cleaved caspase-3 in 33 and 40 mmol/L glucose groups was increased (P<0.05), while the protein expression of Bcl-2 was decreased (P<0.05). No significant difference in the protein levels of PI3K and Akt among the 3 groups was observed, while the protein level of p-Akt in 33 and 40 mmol/L glucose groups was decreased compared with normal control group (P<0.05). Compared with high glucose group, the expression of ROCK1 and ROCK2 was decreased in high glucose+Y27632 group. No significant difference in the protein levels of PI3K and Akt among the 3 groups was observed. Compared with normal control group, the protein level of p-Akt in high glucose group was decreased, and the protein level of p-Akt in high glucose+Y27632 group was increased significantly compared with high glucose group. CONCLUSION Under high glucose environment, ROCK may reduce the level of p-Akt by inhibiting the PI3K/Akt signaling pathway, thus promoting the apoptosis of cardiomyocytes.  相似文献   

Effects of apelin-13 on oxidative stress induced by high uric acid in adipocytes     

ZHANG Jun-xia  LIN Xue  XU Jin-xiu  TANG Feng 《园艺学报》2020,36(1):134-139

AIM: To study the effects of apelin-13 on oxidative stress induced by high uric acid in 3T3-L1 adipocytes and its underlying mechanisms. METHODS: 3T3-L1 adipocytes were stimulated with uric acid at 10 mg/dL for 48 h. Some of the adipocytes were administered with 1 μmol/L apelin-13 in the presence of uric acid at 10 mg/dL. The adipocytes stimulated with 100 μmol/L H₂O₂ were served as positive controls. The intracellular reactive oxygen species (ROS) concentrations were detected by flow cytometry. The biochemical kits were used to measure the activities of superotide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and NADPH oxidase (NOX) activity, and the content of malondialdehyde (MDA) in the cell lysate and the supernatant. The mRNA levels of renin-angiotensin system (RAS) components, including angiotensinogen (AGT), angiotensin-converting enzyrne1 (ACE1), angiotensin II type 1 receptor (AT1R) and AT2R, as well as angiotensin II receptor -like 1 (APJ) were measured by real-time PCR. The concentrations of angiotensin II (AngⅡ) in the cell lysate and the supernatant were measured by ELISA. RESULTS: Adipocytes stimulated with uric acid at 10 mg/dL had lower activities of antioxidant enzymes (SOD, GSH-PX and CAT) and higher levels of NOX activity and MDA content (P < 0.05). Accordingly, the intracellular ROS levels were found to be dramatically increased. However, apelin-13 administration attenuated uric acid-induced oxidative stress in the 3T3-L1 adipocytes. Uric acid at 10 mg/dL upregulated the mRNA expression of local RAS, enhanced AngⅡ concentrations both in the cell lysate and the supernatant, and down-regulated the mRNA level of APJ in the adipocytes (P < 0.05). Conversely, apelin-13 partially reversed these parameters. CONCLUSION: Apelin-13 attenuates oxidative stress induced by uric acid, may be via down-regulation of local RAS expression in the 3T3-L1 adipocytes.  相似文献   

黄连中盐酸小檗碱的提取纯化及抑菌活性研究     

屈欢  黄雪  王军节  杨晨希  张琇 《植物保护》2020,46(2):96-100

为了探究盐酸小檗碱在植物病原真菌方面的抑制作用,以黄连根粉为材料,采用酸水法提取和乙醇重结晶获得盐酸小檗碱纯品,采用生长速率法研究了盐酸小檗碱对常见植物病原菌的抑制活性。结果表明,盐酸小檗碱的提取率为3.3%,经高效液相色谱法测得其含量为93.56%;抑菌活性试验表明,盐酸小檗碱在100μg/mL时对玉米大斑病菌的抑制率达99.9%,EC_(50)为8.20μg/mL,表现出比药剂对照噁霉灵和百菌清更高的抑制活性;进一步的试验表明,与噁霉灵及百菌清相比,盐酸小檗碱对玉米大斑病菌的抑制作用具有持效期长的特点。  相似文献   

马铃薯组培苗时期不同LED光源处理对温室移栽后植株生长和微型薯结薯的影响     

陈丽丽  王浩瑛  宫晓晨  高游慧  薛绪掌  胡跃高 《中国农学通报》2020,36(6):11-18

不同LED光源下培养的马铃薯组培苗在形态特征上差异明显,为探索这种差异是否会对其移栽后产生进一步影响,试验研究了100%红光(R)、100%蓝光(B)、100%绿光(G)、75%红光+25%蓝光(RB)、45%红光+35%蓝光+20%绿光(RBG)和100%白光(CK)6种光谱下培养4周的马铃薯组培苗温室移栽后植株的生长和结薯情况。试验结果表明,B处理的马铃薯组培苗移栽后缓苗期叶绿素荧光参数Fv/Fm和qP、结薯前期SPAD值及结薯期各器官生物量均始终高于CK和其他处理,且该处理下最大薯的干鲜重和有效微型薯占比分别较CK提高45.66%、45.09%和10.93%。R处理的组培苗移栽后结薯期地上部生物量显著高于CK,单株结薯数目提高29.60%,但有效微型薯占比较CK降低63.97%。G处理的组培苗移栽后生长和结薯指标均表现最差。组合光谱RB和RBG处理的组培苗移栽后的生长指标和微型薯产量及产量构成指标均与CK无显著差异。因此,单色红、蓝、绿光谱处理对马铃薯组培苗的影响会持续至其移栽后植株幼苗生长,甚至结薯期,组合光谱RB和RBG则不会。在马铃薯组培苗生产上,单色蓝光和单色红光作为照明光源具有潜在的应用价值,组合光谱RB和RBG可作为白色光源的替代光源。  相似文献   

基于无人机多光谱遥感的冬小麦叶绿素含量反演及监测   总被引：2，自引：2，他引：0  

奚雪  赵庚星 《中国农学通报》2020,36(20):119-126

旨在实现冬小麦各生育期叶绿素含量的准确估测,探究其时空变化规律。利用无人机获取冬小麦越冬期、返青期、拔节期、孕穗期和灌浆期的高分辨率多光谱图像,同时采集地面SPAD数据。选取三类光谱参数建立反演模型,优选出各生育期的最佳预测模型,并定量监测试验区冬小麦叶绿素含量时间变化和空间分布。结果表明：原始波段模型和波段倒数对数模型分别为越冬期及其他生育期叶绿素含量预测的最佳模型,拟合精度R²>0.59;时空分布上,灌浆期前试验区冬小麦叶绿素含量呈南北高、中部低特点,灌浆期则呈北高南低的趋势,叶绿素含量从越冬期到拔节期逐步增加,拔节期到孕穗期开始降低,孕穗期到灌浆期则大幅度降低。本研究建立的倒数对数预测模型,精度较高,且适用于返青到灌浆的4个生育期,对于试验区冬小麦叶绿素含量有较好的时空监测效果。  相似文献   

鸭坦布苏病毒病、鸭圆环病毒病与鸭疫里默氏杆菌混合感染的诊断   总被引：1，自引：0，他引：1  

李婷  徐景峨  杨莉  蒲龄  张亚楠  余波  韩雪  赵滨  杨粤黔 《贵州农业科学》2020,48(8):77-81,封3

为确诊贵州省三穗县某鸭场62日龄花边鸭发病原因,通过临床症状观察及对采集的10只病鸭进行病理剖检、细菌分离鉴定和病毒PCR/RT-PCR检测。结果表明:分离到4株鸭疫里默氏杆菌,对头孢类药物高度敏感,对大环内酯类和氨基糖苷类药物耐药;病毒特异性引物扩增出鸭坦布苏病毒和鸭圆环病毒条带。该鸭场病鸭由鸭坦布苏病毒、鸭圆环病毒和鸭疫里默氏杆菌混合感染所致。  相似文献   

贵州省火龙果炭疽病病原的分离鉴定及生物农药筛选     

赵晓珍  王荔  张雪  郑伟  张荣  秦永华  王正媛  张永忠 《贵州农业科学》2020,48(4):70-74,封2

为贵州省火龙果炭疽病的绿色防控提供理论基础,采用常规组织分离法、形态学与分子生物学鉴定相结合对火龙果病原菌进行分离鉴定,采用生长速率法室内测定9种生物农药对致病菌株的抑菌活性。结果表明:火龙果炭疽病病原为胶孢炭疽菌(Colletotrichum gloeosporioides),0.3%四霉素对火龙果炭疽病菌的抑菌作用最强,EC_(50)为1.331 4 mg/L;3%中生菌素和1%申嗪霉素其次,EC_(50)分别为6.787 2mg/L和7.832 8 mg/L;80%乙蒜素、2%春雷霉素、10%多抗霉素和2%溃腐灵的EC_(50)分别为65.040 1mg/L、71.349 1mg/L、108.378 4mg/L和129.416 6mg/L;20%井冈霉素和4%嘧啶核酸类抗菌素的抑菌作用较差,其EC_(50)分别为1 985.302 4mg/L和3 298.115 0mg/L。  相似文献   

不同小麦品种小麦白粉病发病程度及其田间发生动态分析     

高海峰  杨雪  陈利  白微微  沈煜洋  黄天荣  王剑  范贵强  方辉 《新疆农业科学》2020,57(5):895-901

【目的】研究新冬60号、新冬57号、新冬40号和新冬20号小麦白粉病危害程度和田间发生动态,为科学防控小麦白粉病提供理论依据。【方法】采用定期定点调查的方法,系统调查不同品种小麦白粉病发生情况。【结果】新冬60号、新冬57号、新冬40号小麦白粉病田间发生动态基本一致。新冬60号、新冬57号、新冬40号和新冬20号小麦白粉病病情指数最高分别为8.22、7.11、37.63和25.48。新冬60号和新冬57号小麦白粉病田间发生程度极显著轻于新冬40号和新冬20号,新冬40号小麦白粉病田间危害程度极显著重于新冬20号。【结论】新冬60号和新冬57号小麦白粉病田间危害程度极显著轻于新冬20号,可根据田间发病情况,合理减施农药。新冬40号小麦白粉病田间发生程度极显著重于新冬20号,应加强对小麦白粉病的预防。  相似文献   

食用菌菌包生产现状与发展趋势          下载免费PDF全文

翁晓星  陆丹丹  郑涛  赵晋  林雪  朱建锡  王洁  费焱 《农业工程》2020,10(6):14-17

介绍了食用菌栽培工艺流程及菌包生产国内外研究现状,指出存在的问题及改进措施,并提出食用菌工厂化生产的发展趋势。   相似文献   

Y-S-Y12发酵液和生物质热解液混配对辣椒炭疽病的防治及作用机理     

王薇  李泳  王伟  边雪  李熙英 《中国农业科技导报》2020,22(10):129-138

为探究解淀粉芽孢杆菌Y-S-Y12拮抗菌发酵液和生物质热解液混配对辣椒炭疽病的协同防病作用及其作用机理,对不同浓度生物质热解液、拮抗细菌Y-S-Y12菌株的发酵浓缩液及其混配溶液对辣椒炭疽病的抑菌作用进行检测,计算出毒力方程,根据EC50值将两种溶液进行混配,求得最佳配比。通过活体试验检测Y-S-Y12菌株对辣椒炭疽病果实的防治效果,测定叶片超氧化物歧化酶（superoxide dismutase,SOD)、过氧化物酶（peroxidase,POD）和过氧化氢酶（hydrogen peroxidase,CAT）活性,并探究各药剂对辣椒炭疽病病菌菌丝的细胞膜通透性、生理代谢和菌丝形态的影响。结果显示,Y-S-Y12菌株发酵浓缩液和生物质热解液以1∶9混配具有明显的协同增效作用;混配的防效显著高于单剂,防效达到79.62%,进一步说明混配具有增效作用。混剂处理的辣椒炭疽病菌菌丝中的几丁质酶活性、β-1,3-葡聚糖酶活性和蛋白酶活性显著高于对照和单剂处理,处理后菌体的保护屏障被打破,使培养液的电导率、外渗液中蛋白质和核酸含量上升。混剂处理的辣椒叶片中SOD、POD、CAT酶活性显著高于对照和单剂处理,说明辣椒植株抗病性增强。综上,Y-S-Y12菌株发酵液和生物质热解液混配对辣椒炭疽病有协同防病作用,可能是因为Y-S-Y12菌株具有破坏辣椒炭疽病菌菌丝和诱导辣椒植株抗病性作用。研究为辣椒炭疽病无公害防治提供理论依据。  相似文献