First report of anthracnose on tillandsia caused by Colletotrichum sp. in Japan     

Kumita  Kohei  Kitazawa  Yugo  Tokuda  Ryosuke  Miyazaki  Akio  Maejima  Kensaku  Namba  Shigetou  Yamaji  Yasuyuki 《Journal of General Plant Pathology》2021,87(4):254-258

Journal of General Plant Pathology - In July 2018, tillandsia (Tillandsia sp.) plants with brown to dark brown lesions on the leaves and pseudobulbs were found in Japan. Based on morphological and...  相似文献   

Performance of a new artificial abalone hatchery culture system in terms of settlement of larvae and growth and survival of postlarval Haliotis discus discus (Reeve)     

Adrian E  STOTT  Toshio  TAKEUCHI  Yasuyuki  KOIKE 《Fisheries Science》2004,70(6):1070-1081

ABSTRACT:   A new abalone postlarval culture system, Stott's abalone postlarval production system (SAPPS), is being developed as an alternative method for settling larvae and on-growing postlarval abalone. SAPPS was tested in trial 1 using two commercially available artificial diets, Adam and Amos (Adam; supplied by Adam and Amos, Mt Barker, SA, Australia) and Cosmo (Cos; supplied by Cosmo, Matsuyama, Japan), against the diatom biofilm method (Diatom). In trial 2, SAPPS was tested against the previous production system (PPS, used by Stott et al. 2002). Larvae were induced to metamorphose and the resulting postlarvae were on-grown in 10-L flow-through tanks for 28 days in their respective treatments. The final survival of postlarvae was significantly higher ( P  < 0.05) in the SAPPS-Cos group (56.7 ± 11.15%) than in the Diatom (9.4 ± 2.7%) and SAPPS-Adam groups (8.5 ± 1.1%). The final length of postlarvae in SAPPS-Cos (1065 ± 73 µm) was significantly higher ( P  < 0.05) than those in the other two treatments (average of 841–883 µm). In trial 2 the metamorphosis rate was improved by using Spirulina platensis. The final length of postlarvae in SAPPS (1449–1471 µm) was significantly higher ( P  < 0.05) than PPS (1065–1075 µm) and Diatom (993 µm). There is potential for SAPPS to be used as an alternative to the current diatom method in the culture of postlarval abalone.  相似文献   

Heterologous expression of gassericin A,a bacteriocin produced by Lactobacillus gasseri LA39     

Yasushi KAWAI  Kensuke ARAKAWA  Ayako ITOH  Boku SAITOH  Yasuyuki ISHII  Junko NISHIMURA  Haruki KITAZAWA  Takatoshi ITOH  Tadao SAITO 《Animal Science Journal》2003,74(1):45-51

Gassericin A, a bacteriocin produced by Lactobacillus gasseri LA39, has a cyclic structure linking N‐ and C‐terminal amino acids. Gassericin A was expressed in Escherichia coli JM109 as a biotinylated fusion protein on the basis of the DNA sequence of mature bacteriocin. A positive clone accumulated the bacteriocin, with no activity, as a soluble fusion protein in the cytoplasm. After release of an N‐terminal tag with factor Xa protease, gassericin A was converted into an active peptide having N‐ and C‐termini. The total amount of purified bacteriocins (expressed and native) was 480 µg/L and 370 µg/L, respectively. However, the specific activity of expressed gassericin A was 15 AU/mg lower than that of native bacteriocin (2600 AU/mg). Although the actual Mr (molecular weight) of the expressed bacteriocin should be 5666, the peptide showed the same mobility (Mr 3800) in sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) as native cyclic gassericin A, suggesting that the expressed peptide retains compact folding of the molecule similar to that of native gassericin A.  相似文献   

Enzymatic dehydrogenative polymerization of monolignol dimers     

Yasuyuki Matsushita  Chisato Ko  Dan Aoki  Shota Hashigaya  Sachie Yagami  Kazuhiko Fukushima 《Journal of Wood Science》2015,61(6):608-619

  相似文献   

Near-infrared imaging spectroscopy based on sugar absorption band for melons.   总被引：2，自引：0，他引：2  

Mizuki Tsuta  Junichi Sugiyama  Yasuyuki Sagara 《Journal of agricultural and food chemistry》2002,50(1):48-52

A method for visualizing the sugar content in the flesh of melons was developed. This method was based on the sugar absorption band in the near-infrared (NIR) region to avoid bias caused by the color information of a sample. NIR spectroscopic analysis revealed that each of the two second-derivative absorbances at 874 and 902 nm had a high correlation with the sugar content of melons. A high-resolution cooled charged couple device camera with band-pass filters, which included the above two wavelengths, was used to capture the spectral absorption image of a half-cut melon. A color distribution map of the sugar content on the surface of the melon was constructed by applying the NIR spectroscopy theory to each pixel of the acquired images.  相似文献   

Effects of the application of charred bark of Acacia mangium on the yield of maize, cowpea and peanut, and soil chemical properties in South Sumatra, Indonesia   总被引：2，自引：0，他引：2  

Masahide YAMATO  Yasuyuki OKIMORI  Irhas Fredy WIBOWO  Saifuddin ANSHORI  Makoto OGAWA 《Soil Science and Plant Nutrition》2006,52(4):489-495

  相似文献   

Antioxidant and cytotoxic activities of naphthalene derivatives from <Emphasis Type="Italic">Diospyros kaki</Emphasis>     

Yasuyuki Matsushita  In-Cheol Jang  Takanori Imai  Kazuhiko Fukushima  Jeung-Min Lee  Hae-Ryong Park  Seung-Cheol Lee 《Journal of Wood Science》2011,57(2):161-165

4,8-Dihydroxy-5-methoxy-2-naphthaldehyde (Compound I) was isolated from blackened heartwood of Diospyros kaki and was methylated with diazomethane. Antioxidant and cytotoxic activities of Compound I and two methylated derivatives [4-hydroxy-5,8-dimethoxy-2-(2-oxopropyl)-naphthalene (D-1) and 2-glycidyl-4-hydroxy-5,8-dimethoxy naphthalene (D-2)] were evaluated. Compound I showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and reducing power than D-1 and D-2. However, D-1 and D-2 exhibited slightly stronger 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)⁺ (ABTS⁺) radical scavenging activity than Compound I. Compound I also exhibited stronger cytotoxic activity than D-1 and D-2 against the growth of HT-29 colon cancer cells. The results supported the hypothesis that methylation of naphthalene derivatives slightly increased ABTS⁺ radical scavenging activity, but significantly decreased DPPH radical scavenging activity, reducing power, and cytotoxic activity.  相似文献   

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs     

Hiroshi SUZUKI  Hiroyuki WATANABE  Yasuyuki ABE 《The Journal of reproduction and development》2022,68(1):1

Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock, and for the treatment of human infertility. Their widespread use has contributed to improvements in human health and welfare. However, in dogs, only artificial insemination using frozen semen is readily available as an ART to improve breeding and control genetic diversity. A recent priority in sperm cryopreservation is the development of alternatives to egg yolk, which is widely used as a component of the sperm extender. Egg yolk can vary in composition among batches and is prone to contamination by animal pathogens. The latter can be a problem for international exchange of cryopreserved semen. Low-density lipoprotein and skim milk are promising candidates for use as extenders, to ensure fertility after artificial insemination. Although not tested for its effects on fertility following artificial insemination, polyvinyl alcohol may also be a useful alternative to egg yolk as an extender. The development of cryopreservation techniques for canine embryos lags behind that for other mammals, including humans. However, given the success of non-surgical embryo transfer in 2011, studies have sought to refine this approach for practical use. Research on sperm cryopreservation has yielded satisfactory results. However, investigation of other approaches, such as cryopreservation of oocytes and gonadal tissues, remains insufficient. Techniques for the efficient induction of estrus may aid in the development of successful canine ARTs.  相似文献   

Mercury levels of small fishes: influence of size and catch area     

Wen Jye Mok  Manabu Seoka  Yasuyuki Tsukamasa  Ken-ichi Kawasaki  Masashi Ando 《Fisheries Science》2011,77(5):823-828

In the present study, we assessed small fishes as potential feed fishes with the lowest mercury levels. The mercury levels of four small pelagic fishes and a benthic fish from the Pacific Ocean and the Sea of Japan, i.e., spotted chub mackerel Scomber australasicus, chub mackerel Scomber japonicus, horse mackerel Trachurus trachurus, round scad Decapterus punctatus, and bastard halibut Paralichthys olivaceus, were determined. Total mercury levels in fish muscle were measured using a reduction vaporizing atomic absorption method. There were no significant correlations between body weight and mercury levels for these species. However, significant differences were observed in the mercury levels of these species between the two catch locations, with fish caught in the Pacific Ocean having higher mercury levels. This difference may be due to the Pacific Ring of Fire, a highly seismically and volcanically active zone, which is located in the Pacific Ocean. Preference for these fishes for use in aquaculture should refer to the area where they are caught in addition to the species.  相似文献   

Estimation of heritability of vertebral number in chum salmon <Emphasis Type="Italic">Oncorhynchus keta</Emphasis>     

Daisei Ando  Mizue Murooka  Kazutaka Shimoda  Hirofumi Hayano  Yoshitaka Sasaki  Yasuyuki Miyakoshi  Masamichi Nakajima 《Fisheries Science》2017,83(3):413-423

Hereditary causes of variation in the vertebral number of chum salmon were analyzed to estimate heritability for populations in the Chitose and Shikiu Rivers, Hokkaido Island, northern Japan. A total of 70 families were produced by diallel cross mating and then heritability was estimated using two statistical tests: a two-way ANOVA and a parent-offspring regression. Heritability estimates ranged from 0.26 to 1.91 and showed a strong correlation between the values from the two different tests. Estimates from female components were substantially higher than those from male components, which can be attributed to the maternal effect. Heritability in the Shikiu River population was shown to be higher than that in the Chitose River population. These results indicate that the heritability of vertebral number in chum salmon is generally high, and also that vertebral number is controlled by hereditary causes.  相似文献