Effect of meat-bleaching and dilution-precipitation procedures on the removal of cathepsin L-like contained in the actomyosin of various fish species     

Yaqin Hu  Katsuji Morioka  Yoshiaki Itoh 《Fisheries Science》2008,74(3):696-698

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尼泊尔Terai地区小规模农场的奶牛和水牛的饲料品质、营养水平及产奶性能     

宋德平  罗士津  瞿明仁 《江西饲料》2006,(4):28-31

调查尼泊尔Terai地区两个村庄(A、B)中20个小农场的泌乳奶牛和水牛以确定其饲料品质、产奶量和营养水平。在牧草充足期、牧草枯萎期和饲草短缺期三个时期中分别测定饲料的组成成分、干物质、奶牛的体质条件(BCS)、胸围(HG)、体重(BW)、产奶量(MY)及血浆代谢物。305d的产奶量通过平均日产奶量来估计。牧草充足期的稻草和当地牧草供应比牧草枯萎期少而比牧草短缺期多,分别为5.5kg/d∶9.8kg/d和3.2kg/d∶0.4kg/d,(P<0.01),A村中的饲料中粗纤维含量比B村高,奶牛中为5.0∶2.2,水牛中为9.3∶1.8(P<0.01)。两个村中各时期的饲料组成的差异导致粗蛋白(CP)、中性洗涤纤维(NDF)、总可消化营养物质(TDN)供应的差异。奶牛饲料的CP和TDN浓度在牧草充足期比其它两个时期高,分别为9.1%∶7.3%和57.4%∶51.0%(P<0.01)。A村中供应给奶牛和水牛的CP和供应给水牛的TDN比B村的高,分别为7.5%∶8.7%和6.6%∶9.7%(P<0.01)以及53.1%∶56.2%(P<0.05)。A村中奶牛和水牛的BCS、HG、BW比B村中的低,奶牛分别为2.51∶2.86,156cm∶170cm,300kg∶318kg,水牛为2.83∶4.00,186cm∶216cm,429kg∶531kg(P<0.01)。奶牛在牧草充足期比其他两期的产奶量大,7.9L/d∶6.6L/d(P<0.01)。奶牛在饲草短缺期产后305d的产奶量比其它两期低,1900L∶2251L(P<0.01)。A村中的奶牛和水牛的产奶量都比B村的低6.2L/d∶8.1L/d,3.7L/d∶7.7L/d(P<0.01)。A村中奶牛305d的产奶量比B村低,1935L∶2409L(P<0.01),A村中奶牛的血浆清蛋白和尿氮比B村的低,3.2g/dL∶3.4g/dL(P<0.01),7.4mg/dL∶10.2mg/dL(P<0.05)。在不同时期两个村中饲料中的CP、NDF、TDN可能会对奶牛和水牛的产奶量和营养水平有影响。似乎在饲草短缺期A村对奶牛低水平供应的CP和TDN使得奶牛305d的产奶量更低。  相似文献   

Efficacy of atovaquone against Babesia gibsoni in vivo and in vitro     

Matsuu A  Koshida Y  Kawahara M  Inoue K  Ikadai H  Hikasa Y  Okano S  Higuchi S 《Veterinary parasitology》2004,124(1-2):9-18

The therapeutic efficacy of atovaquone against Babesia gibsoni was examined in three dogs experimentally infected with B. gibsoni isolated from naturally infected dogs in Aomori Prefecture, Japan. Once parasitemia reached 10%, atovaquone was administered orally (30 mg/kg twice daily for 7 days). Within 2 days of atovaquone treatment, the parasite disappeared from blood smears without any clinical side effects. Anemia and thrombocytopenia were significantly improved in all the dogs. However, a polymerase chain reaction assay revealed that a B. gibsoni marker gene was intermittently present in peripheral blood after atovaquone therapy, indicating that the organism had not been eliminated, and parasites reappeared in blood smears 33 days after the last treatment. To investigate the change in sensitivity against atovaquone, an in vitro sensitivity test was performed using peripheral blood obtained from an untreated dog that was infected with the original parasite isolate, and from two of the experimentally infected and atovaquone-treated animals (blood was collected at the time of the post-treatment recurrence of the B. gibsoni infection). Atovaquone was added to the culture medium to final concentrations of 0.1, 1, 10, 100, and 1000 nM. For the untreated parasites, complete growth inhibition occurred at 1000 nM of atovaquone, whereas the recurrent parasites were inhibited by only 39.52 +/- 8.34% and 31.31 +/- 8.14% at this concentration after 48 h of incubation. Thus, the recurring parasites were less sensitive to atovaquone than the untreated originally isolated parasites.  相似文献   

Potential Mechanisms of Aberrant DNA Hypomethylation on the X Chromosome in Uterine Leiomyomas     

Shun SATO  Ryo MAEKAWA  Yoshiaki YAMAGATA  Hiromi ASADA  Isao TAMURA  Lifa LEE  Maki OKADA  Hiroshi TAMURA  Norihiro SUGINO 《The Journal of reproduction and development》2014,60(1):47-54

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Efficacy of Malarone? in Dogs Naturally Infected with Babesia gibsoni     

Aiko IGUCHI  Nobuyuki SHIRANAGA  Aya MATSUU  Yoshiaki HIKASA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(9):1291-1295

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Antagonistic Effects of Atipamezole,Yohimbine and Prazosin on Medetomidine-Induced Diuresis in Healthy Cats     

Yusuke MURAHATA  Asami YAMAMOTO  Yuya MIKI  Yoshiaki HIKASA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(2):173-182

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Effects of in utero and lactational exposure to the no-observed-adverse-effect level (NOAEL) dose of the neonicotinoid clothianidin on the reproductive organs of female mice     

Sayaka KITAUCHI  Mizuki MAEDA  Tetsushi HIRANO  Yoshinori IKENAKA  Misaki NISHI  Asuka SHODA  Midori MURATA  Youhei MANTANI  Toshifumi YOKOYAMA  Yoshiaki TABUCHI  Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):746

Recently, developmental exposure to clothianidin (CLO) has been shown to cause reproductive toxicity in male mice, but the effects in female mice remain to be clarified. Pregnant C57BL/6N mice were given a no-observed-adverse-effect-level (NOAEL) dose of CLO until weaning. We then examined ovaries of 3- or 10-week-old female offspring. In the CLO-administered group, morphological changes, a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 (GPx4), and activation of genes in the steroid hormone biosynthesis pathway were observed in 3-week-old mice, and decreases of GPx4 immunoreactivity, 17OH-progesterone and corticosterone levels were observed in 10-week-old mice, along with high rates of infanticide and severe neglect, providing new evidence that developmental exposure to CLO affects juvenile and adult mice differently.  相似文献   

Effect of pH-shifting on the gel forming characteristics of salt-ground meat from walleye pollack     

Fatema Hoque Shikha  Mohammed Ismail Hossain  Katsuji Morioka  Satoshi Kubota  Yoshiaki Itoh 《Fisheries Science》2006,72(4):870-876

ABSTRACT:   In order to elucidate the mechanism of the changes in gel forming characteristics of fish meat by pH-lowering, the gelation-temperature curve and the gelation-moisture content curve were examined using the acidified walleye pollack surimi or neutralized one after acidification. In the gelation-temperature curve, the gel strength was highest at 30°C and lowest at approximately 50–60°C, irrespective of pH shifting. The gel strength at 30°C and 80°C decreased with the decrease in pH value. The neutralization of acidified surimi improved the gel strength, but it was considerably lower than the original gel strength. The gel strength at 50–60°C was not affected by pH lowering. The gel strength at 80°C could not be revived to the original by pH readjustment, either in the presence or in the absence of EDTA. These results suggest that irreversible changes of meat protein take place under the low pH, and the oxidation ability of sulfhydryl (SH) groups of protein molecule is not affected by pH-shifting.  相似文献   

Antihypertensive effect of Narezushi, a fermented mackerel product, on spontaneously hypertensive rats     

Kouji Itou  Risa Nagahashi  Miho Saitou  Yoshiaki Akahane 《Fisheries Science》2007,73(6):1344-1352

ABSTRACT:   During the fermentation of mackerel to narezushi , the concentration of peptides required to inhibit 50% of the ACE activity in the assay media (IC₅₀), as an index of the angiotensin  I-converting enzyme (ACE) inhibitory activity, was remarkably decreased with a rapid increase in peptide contents. Systolic blood pressure (SBP) in spontaneously hypertensive rats (SHR) decreased between 2 and 4 h after the single oral administration of greater than 10 mg peptide/kg narezushi extract, and recovered to the initial level by 8 h thereafter. The SBP decreased at seven successive daily doses of 10 mg/kg of narezushi extract and then recovered to the initial level 5 days after stopping a total of 10 daily administrations. The extract was administered to five-week-old SHR rats for 70 days and SBP decreased 21 days after starting and continued for 28 days after the end of administration. The peptide-rich fraction from narezushi extract had a powerful antihypertensive effect, whereas the other fraction had a similar, but weak effect.  相似文献   

Expression analysis of the type I keratin protein keratin 33A in goat coat hair     

Michinari YOKOHAMA  Kenta WADA  Masaru FUJITA  Mai KOTANI  Yoshio NAGURA  Masako KANNO  Kou NOMURA  Takashi AMANO  Yoshiaki KIKKAWA 《Animal Science Journal》2011,82(6):773-781

The coat of a goat, like that of many mammalian species, consists of an outer coat of coarse hairs and an under coat of fine, downy hairs. The coarse guard hairs are produced by primary follicles and the finer cashmere hairs by secondary follicles. We previously reported that hair keratins are components of cashmere hair, and proteomic analysis revealed that their expression is elevated in winter coat hair. To determine detailed characterization, we have cloned keratin 33A gene, a major highly expressed keratin in winter, and then analyzed the expression of goat hair coat. By Western analysis, we detected that keratin 33A protein is expressed only in hair coat among the various goat tissues. Moreover, the expression level in winter has increased in cashmere high‐producing Korean native breed, whereas the expression levels between summer and winter had not changed in cashmere low‐producing Saanen. In addition, by immunohistochemistry we determined that keratin 33A is localized in the major cortex portion of cashmere fiber. These results confirm that keratin 33A is a structural protein of goat cashmere hair fiber.  相似文献