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71.
It has been well known that the inorganic nitrogen compounds used as the common nitrogen source for the growth of higher plants can be replaced by some organic nitrogen compounds such as amino acids or amides. According to GHOSH and BURRIS (1), who investigated the effect of some amino acids as the nitrogen source, alanine, asparagine, glutamate and histidine were better nitrogen sources than ammonia for clover and tomato plants. For tobacco, however, nitrate and ammonia were superior to all organic nitrogen compounds used. RATNER et al. (2). made the same kind of study by using corn and sunflower plants and reported that the plants could grow with glycine, aspartate, glutamate and arginine, but all of them were inferior to inorganic nitrogen as the nitogen source.  相似文献   
72.
Nitrogen nutrition has great influences not only on the yields, but also on the chemical components of the plants. Nitrate nitrogen is generally found to be superior to ammonium nitrogen as the nitrogen source for higher plants (1). As reported in the previous paper (2), young plants of sugar beet grown with the culture solution containing a high level of ammonium nitrogen developed the symptom of ammonia toxicity. To make clear the mechanism of ammonia toxicity, comparative studies on the absorption and the assimilation of ammonium- and nitrate-nitrogen in higher plants at young stages of growth were carried out.  相似文献   
73.
In Japan anthropogenic-alluvial soils excluding irrigated rice soils are lagging for behind other genetic soil types in pedological investigations. Although there are some investigations of anthropogenic-alluvial soils used for mulberry plantations, orchards, vegetable gardens, and seeding beds for trees, the main object of these investigations is not pedological. Kanno et al. (1) reported that unirrigated anthropogenic-alluvial soils used for vegetable growing in the Chikugo Plain resemble the “Alochthonous brown warp soil” defined by Kubiena (2). First of all, it is of great importance to accumulate general data for unirrigated anthropogenic-alluvial soils and to develop the classification principles for them. Classification problems will be settled in accordance with the accumulated data. This paper deals with characteristics of an unirrigated anthorpogenic-alluvial soil found in the Kumamoto Plain and with classification principles for it. These principles have almost never been described for a pedological standpoint in Japan.  相似文献   
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为研究鲤疱疹病毒Ⅱ型(Cy HV-2)体外感染复制特征以及异育银鲫抗病毒免疫应答反应。本实验采用组织块培养法建立了异育银鲫背鳍细胞的原代培养体系。结果显示,在10 d左右可观察到组织块迁移分离出新的单层细胞,3周左右细胞可覆盖底部面积为25cm2培养瓶的底部;经Cy HV-2悬液感染离体培养的原代细胞,3 d后病毒滴度增殖至106拷贝/m L;在病毒感染6 d后出现典型的细胞病变效应;Cy HV-2感染原代细胞后,分析前期通过鱼体水平实验鉴定出的与该病毒感染相关的免疫基因:PNP5a、MPO、MHCⅠ、LYZ-C、IL-11、ITLN、PNP5a和DUSP,Real-time Rt-PCR结果显示大部分基因在细胞水平均有显著性的上调,与鱼体水平实验结果一致。本研究建立了原代培养的异育银鲫背鳍细胞,用于构建体外感染Cy HV-2病毒的细胞模型,为深入研究Cy HV-2的感染复制规律及其与宿主的相互作用关系,以及细胞水平筛选抗病毒药物实验奠定了基础。  相似文献   
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The effects of heat exposure and type of diet on the insulin secretory response to glucose and glucose disposal in response to insulin action in female sheep were investigated employing hyperglycemic and euglycemic clamp techniques. Animals were divided into concentrate and roughage diet groups, and were maintained at the same intake levels of metabolizable energy and crude protein in both diets. Each diet group was subjected to either thermoneutral (20°C, 70% RH) or hot (30°C, 70% RH) environment, followed by glucose clamp experiments.

Heat-exposed sheep showed significant increases in respiration rates (P<.001) and rectal temperature (P<.05). Plasma glucose concentrations in the basal conditions were lower (P<.01) in the hot environment than in the thermoneutral environment, but there was no significant difference in basal levels of plasma insulin between the environmental treatments. In the hyperglycemic clamp experiment, mean plasma insulin increments increased (P<.05) during the heat exposure period across diet treatments. The ratio of mean plasma insulin increment to glucose infusion rate tended to be higher (P<.07) in the hot environment than in the thermoneutral environment, but diet treatment did not affect the ratio of mean plasma insulin increment to glucose infusion rate. The euglycemic clamp technique showed that glucose infusion rates remained unchanged among treatments. Insulin secretion response to glucose could be stimulated in the hot environment.  相似文献   

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Kazakhstan is the largest landlocked country and contains two important propagation routes for livestock from the Fertile Crescent to Asia. Therefore, genetic information about Kazakhstani cattle can be important for understanding the propagation history and the genetic admixture in Central Asian cattle. In the present study, we analyzed the complete mtDNA D‐loop sequence and SRY gene polymorphism in 122 Kazakhstani native cattle. The D‐loop sequences revealed 79 mitochondrial haplotypes, with the major haplogroups T and I. The Bos taurus subhaplogroups consisted of T (3.3%), T1 (2.5%), T2 (2.5%), and T4 (0.8%) in addition to the predominant subhaplogroup T3 (86.9%), and the Bos indicus subhaplogroup of I1 (4.1%). Subsequently, we investigated the paternal lineages of Bos taurus and Bos indicus, however, all Kazakhstani cattle were shown to have Y chromosome of Bos taurus origin. While highly divergent mtDNA subhaplogroups in Kazakhstani cattle could be due to the geographical proximity of Kazakhstan with the domestication center of the Fertile Crescent, the absence of Bos indicus Y chromosomes could be explained by a decoupling of the introgression dynamics of maternal and paternal lineages. This genetic information would contribute to understanding the genetic diversity and propagation history of cattle in Central Asia.  相似文献   
80.
Goats (Capra hircus) were domesticated in the Fertile Crescent and propagated all over the world. The Silk Road through the Eurasian Steppe belt is a possible propagation route for domestic goats to Central Asia. Kazakhstan is in close geographical proximity to domestication centers and covers the majority of the Eurasian Steppe belt. In this study, we examined the genetic diversity and phylogeographic structure of Kazakhstani goats. The mtDNA sequences of 141 Kazakhstani goats were categorized into haplogroups A, C, and D, of which haplogroup A was predominant (97%), whereas haplogroups C and D were detected at low frequencies (1.4% each). The Kazakhstani haplotypes C were thzen categorized into Asian mtDNA type. Sequence analysis of the SRY gene on the Y‐chromosome in 67 male Kazakhstani goats revealed two haplotypes: Y1A (64%) and Y2A (36%). Analysis of the distribution of mtDNA haplogroups and SRY haplotypes from Eurasia and Africa demonstrated genetic similarity among animals from Kazakhstan, Mongolia, and Northwest China located on the Eurasian Steppe belt. These phylogeographic results suggested that the Eurasian Steppe belt was an important propagation route for goats to Central Asia.  相似文献   
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