Antimicrobial Activity of Culture Filtrate of Bacillus amyloliquefaciens RC-2 Isolated from Mulberry Leaves   总被引：1，自引：0，他引：1  

Yoshida S  Hiradate S  Tsukamoto T  Hatakeda K  Shirata A 《Phytopathology》2001,91(2):181-187

ABSTRACT A potential antagonist, Bacillus amyloliquefaciens strain RC-2, against Colletotrichum dematium, mulberry anthracnose fungus, was obtained from healthy mulberry leaves by in vitro and in vivo screening techniques. Application of culture filtrate of RC-2 inhibited disease on mulberry leaves, indicating that suppression was due to antifungal compounds in the filtrate. Development of mulberry anthracnose on mulberry leaves was inhibited only when the culture filtrate was applied before fungal inoculation, and it was not inhibited by application after inoculation. These results suggest that the antifungal compounds in the filtrate exhibit a preventive effect on the disease. Peptone significantly increased production of the antifungal compounds. The culture filtrate of RC-2 also inhibited the growth of several other phytopathogenic fungi and bacteria, such as Rosellinia necatrix, Pyricularia oryzae, Agrobacterium tumefaciens, and Xanthomonas campestris pv. campestris, in vitro. From the culture filtrate of RC-2, seven kinds of antifungal compounds were isolated by high performance liquid chromatography analysis, and one of the compounds was determined as iturin A2, a cyclic peptide, by nuclear magnetic resonance and fast atom bombardment mass analysis.  相似文献   

Invasion and colonization of mature apple fruit by Erwinia amylovora tagged with bioluminescence genes     

Koji Azegami  Takanori Tsukamoto  Takayuki Matsuura  Tatsuji Ohara  Yasuhiro Inoue  Akifumi Mizuno  Kouji Yoshida  Hideo Bessho  Shigeru Kimura  Masao Goto 《Journal of General Plant Pathology》2005,71(1):98-98

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Restriction fragment length polymorphism as a genetic marker of the c-myc oncogene in rats     

T Kunieda  M Matsui  N Nomura  R Ishizaki  M C Yoshida  T Imamichi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(6):1295-1298

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In vitro fertilization of pig oocytes matured in vivo     

M Yoshida 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(4):711-718

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Pharmacological studies on doping drugs for race horses. IV. Chlorpromazine and phenobarbital (author's transl)]     

S Fujii  S Inada  S Yoshida  C Kusanagi  K Mima 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1975,37(2):133-139

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Generation of canine dendritic cells from peripheral blood mononuclear cells     

Yoshida H  Momoi Y  Taga N  Ide K  Yamazoe K  Iwasaki T  Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):663-669

Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 x 10(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.  相似文献   

Successful treatment of two dogs with allergic dermatitis by anti-allergic peptides (MS-antigen)     

Park SJ  Yoshida N  Nishifuji K  Sekiguchi M  Momoi Y  Fukada T  Iwasaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(1):63-65

The effects of non-specific immunotherapy with anti-allergic peptides extracted from the urine of human allergic patients (MS-antigen), in two dogs with allergic dermatitis (AD) have been described. Clinically, severe pruritus accompanied by secondary bacterial pyoderma did not respond to conventional therapy with systemic antibiotics. The first clinical change appeared as a significant reduction in pruritus within 3 months, around the time of the 15th injection in both cases. The clinical condition was stabilized after 5 months, allowing the gradual withdrawal of concurrent therapies and an increase of injection intervals. The correlation between the results of intradermal skin tests before and after treatment and the improvement of clinical signs was not obvious.  相似文献   

A 10-year survey of antimicrobial susceptibility of streptococcus suis isolates from swine in Japan   总被引：7，自引：0，他引：7  

Kataoka Y  Yoshida T  Sawada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(10):1053-1057

A number of 689 Streptococcus suis isolates collected nationwide from diseased and healthy pigs from 1987 to 1996 were surveyed for antibiotic susceptibilities to 11 drugs. No isolates resistant to amoxicillin, chloramphenicol, and sulfamethoxazole/trimethoprim were found. Isolates were highly susceptible to penicillins (penicillin G, ampicillin, and amoxicillin) except cloxacillin. They were not susceptible to tetracycline, streptomycin, and kanamaycin (MIC90 50 microg/ml, > or = 100 microg/ml, and > or = 100 microg/ml, respectively). Multiple-resistant isolates (> or = 3 antimicrobial agents) were found in 20.3% of all isolates tested.  相似文献   

Gene constitution of South-East Asian native chickens, commercial chickens and jungle fowl using polymorphisms of four calpain genes     

Fumihiko OKUMURA  Takeshi SHIMOGIRI  Kotaro KAWABE  Shin OKAMOTO  Masahide NISHIBORI  Yoshio YAMAMOTO  Yoshizane MAEDA 《Animal Science Journal》2006,77(2):188-195

The gene constitution of polymorphisms of the four calpain genes (µ‐calpain, m‐calpain, p94, and µ/m‐calpain) were analyzed in South‐East Asian native chickens, White Leghorn and Broiler commercial chickens, and Red and Green jungle fowl. Polymorphisms were detected at all loci in chickens and Red jungle fowl, but only for CAPN1 (µ‐calpain gene) in Green jungle fowl. CAPN2 and CAPN1.5 are linked on chicken chromosome 3, and the genotype for these loci were treated as haplotype. Some combinations of calpain loci were tested using principal component analysis, and the best combination (CAPN1, CAPN3, and CAPN1.5) was determined. The proportion of polymorphic loci (P_poly) and heterozygosity (H?) were 1.00 and 0.316–0.465 in domestic chickens and red jungle fowl, and 0.33 and 0.137 in Green jungle fowl, respectively. G_ST values suggested that the degree of subdivision among native chickens was relatively low except for Thailand, which was highest. Pair‐wise F_ST testing, dendrogram and principal component analysis from the results of calpain loci showed that the four South‐East Asian native and commercial chicken populations were close genetically.  相似文献   

Fungal cell wall degrading enzyme-producing bacterium enhances the biocontrol efficacy of antibiotic-producing bacterium against cabbage yellows     

Someya  N.  Tsuchiya  K.  Yoshida  T.  Noguchi  M. T.  Akutsu  K.  Sawada  H. 《植物病害和植物保护杂志》2007,114(3):108-112

Journal of Plant Diseases and Protection - The antagonistic rhizobacterium, Pseudomonas fluorescens strain LRB3W1, controls cabbage Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans...  相似文献