The distribution and early growth of juvenile Pacific bluefin tuna Thunnus orientalis around Sado Island in the eastern Sea of Japan     

Tanaka  Hiroshige  Kodama  Taketoshi  Suzuki  Nobuaki  Mochizuki  Yosuke  Ashida  Hiroshi  Sato  Takuya  Takeshima  Hirohiko  Nohara  Kenji 《Fisheries Science》2020,86(6):1019-1028

Fisheries Science - The distribution and early growth of juvenile Pacific bluefin tuna (PBF) Thunnus orientalis were studied based on trawl surveys conducted around Sado Island, eastern Sea of...  相似文献   

Interspecific hybrids betweenAllium fistulosumandAllium schoenoprasumreveal carotene-rich phenotype     

Mikihisa Umehara  Takayuki Sueyoshi  Katsumi Shimomura  Masatoshi Iwai  Masayoshi Shigyo  Keita Hirashima  Takao Nakahara 《Euphytica》2006,148(3):295-301

Interspecific hybridization is an effective method to generate a new crop that gains available functions in a short time. Interspecific hybrids (2n= 16) betweenAllium fistulosumL. (2n= 16) andAllium schoenoprasumL. (2n= 16) were produced by reciprocal crossings through ovary culture, but the hybrids were much fewer in the combination usingA. schoenoprasumas a seed plant. All the hybrids have eight long chromosomes originated fromA.fistulosumand eight short chromosomes originated fromA. schoenoprasum. In addition, the hybridity was confirmed by randomly amplified polymorphic DNA (RAPD) analysis and cleaved amplified polymorphic sequence (CAPS) analysis of the rDNA internal transcribed spacer (ITS) region. The interspecific hybrids showed a vigorous growth habit; their foliage was slightly bloomy and deep green. The hybrids did not form bulbs, but rather propagated vegetatively by tillering. Carotene contents of the hybrids and both parents were quantified using high-performance liquid chromatography (HPLC). The contents of all edible parts of the hybrids were approximately seven times higher than those of either parent. These results indicate that the hybrid is a new and carotene-rich vegetable ofAlliumspecies.  相似文献   

Mapping of quantitative trait loci for Melon yellow spot virus resistance in cucumber (Cucumis sativus L.)     

Mitsuhiro Sugiyama  Yoichi Kawazu  Nobuko Fukino  Yosuke Yoshioka  Koichiro Shimomura  Yoshiteru Sakata  Mitsuru Okuda 《Euphytica》2015,205(2):615-625

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Glucocorticoid sensitivity depends on expression levels of glucocorticoid receptors in canine neoplastic mast cells     

Matsuda A  Tanaka A  Amagai Y  Ohmori K  Nishikawa S  Xia Y  Karasawa K  Okamoto N  Oida K  Jang H  Matsuda H 《Veterinary immunology and immunopathology》2011,144(3-4):321-328

Glucocorticoid (GC) administration with or without other chemotherapeutic reagents is a commonly used option in the treatment of mast cell malignancies. However, the responsiveness of mast cell tumors to GC treatment varies in individuals, and the regulatory mechanisms determining the GC sensitivity of malignant mast cells remain unclear. Since the expression of the GC receptor (GR) has been reported to be associated with GC sensitivity in human neoplastic lymphocytes, we attempted to investigate the relationship between GR levels and GC sensitivity by using neoplastic mast cells derived from canine mast cell tumors (MCTs). To elucidate the regulatory mechanisms involved in GC responsiveness, we analyzed various canine MCT cell lines and tissue samples from dogs with MCT. While the proliferation of canine MCT cells was suppressed by the addition of GC to the culture, we found that MCT cells derived from humans and rodents, as well as canine lymphoma cells, responded poorly to GC. However, there were also some variations in responsiveness to GC treatment among canine MCT cell lines used in this study. Using real-time polymerase chain reaction and Western blot analysis, we elucidated the relationship between GR expression and responsiveness to GC in canine MCT cells. Furthermore, to assess the involvement of GR expression in GC sensitivity in vivo, clinical investigations were conducted on dogs with cutaneous MCT. Written informed consent was obtained from owners, and the affected dogs were treated with prednisolone (0.5-2.0 mg kg(-1)day(-1), administered orally) 1 or 2 weeks prior to the surgical removal of the tumors. Tumor volume was measured according to WHO criteria both before and after prednisolone treatment, and the GC sensitivity of each MCT was determined on the basis of the reduction in tumor volume. Of the 15 dogs with MCT, 11 responded to treatment with prednisolone completely or partially, whereas 4 dogs showed no response. Examination of clinical samples obtained by surgical removal revealed that GR expression levels were significantly lower in GC-resistant MCT tissues than in GC-sensitive MCT tissues. Thus, these results strongly indicate that GR expression may contribute to GC sensitivity in canine MCT.  相似文献   

Associations between age of gilts at first mating and lifetime performance or culling risk in commercial herds   总被引：1，自引：0，他引：1  

Saito H  Sasaki Y  Koketsu Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(5):555-559

Age of gilts at first mating (AFM) is a factor associated with reproductive performance of female pigs. The objectives of the present study were to compare AFM and reproductive performance across parity between three herd groups based on a productivity measurement and to determine lifetime performance by AFM and the herd groups. The female data included 38,212 mated gilts entered between 2001 and 2003, and the herd data included mean measurements from 2001 to 2006 in 101 herds. The average female inventory of the 101 herds was 370.2 females. Females were categorized into five groups: AFM 188-208, 209-229, 230-250, 251-271 or 272-365 days. Three herd groups were formed on the basis of the upper and lower 25th percentiles of pigs weaned per mated female over six years: high-, intermediate- and low-performing herds. Multilevel mixed-effects models were performed to analyze comparisons. The AFMs (± SEM) in the high-, intermediate- and low-performing herds were 239.5 ± 0.22, 247.4 ± 0.21 and 256.7 ± 0.35 days, respectively. As the AFM increased from 209-229 to 272-365 days, annualized lifetime pigs born alive (PBA) decreased from 18.2 to 15.3 pigs, and the number of parities at removal decreased from 4.8 to 4.1 (P<0.05). In parity 1, females with an AFM of 209-229 days had fewer PBA, but had a lower culling risk and shorter weaning-to-first mating interval than those with an AFM of 251-271 days (P<0.05). In conclusion, we recommend management practices such as boar exposure to hasten puberty in gilts and decrease AFM.  相似文献   

Expanding the genetic code of Escherichia coli with phosphoserine     

Park HS  Hohn MJ  Umehara T  Guo LT  Osborne EM  Benner J  Noren CJ  Rinehart J  Söll D 《Science (New York, N.Y.)》2011,333(6046):1151-1154

O-Phosphoserine (Sep), the most abundant phosphoamino acid in the eukaryotic phosphoproteome, is not encoded in the genetic code, but synthesized posttranslationally. Here, we present an engineered system for specific cotranslational Sep incorporation (directed by UAG) into any desired position in a protein by an Escherichia coli strain that harbors a Sep-accepting transfer RNA (tRNA(Sep)), its cognate Sep-tRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep). Expanding the genetic code rested on reengineering EF-Tu to relax its quality-control function and permit Sep-tRNA(Sep) binding. To test our system, we synthesized the activated form of human mitogen-activated ERK activating kinase 1 (MEK1) with either one or two Sep residues cotranslationally inserted in their canonical positions (Sep(218), Sep(222)). This system has general utility in protein engineering, molecular biology, and disease research.  相似文献   

Fungal N₂O production in an arable peat soil in Central Kalimantan, Indonesia     

Yosuke YANAI  Koki TOYOTA  Tomoaki MORISHITA  Fumiaki TAKAKAI  Ryusuke HATANO  Suwido H. LIMIN  Untung DARUNG  Salampak DOHONG 《Soil Science and Plant Nutrition》2007,53(6):806-811

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Isolation of endophytic streptomycetes from above- and belowground organs of Quercus serrata     

W. Thongsandee  Yosuke Matsuda  M. Shimizu  H. Ehara  S. Ito 《Journal of Forest Research》2013,18(2):179-189

The occurrence and localization of endophytic actinomycetes within diverse organs of host plants provide ecological information that can be used to evaluate the significance of their spatial habitats. The aim of this study was to isolate and characterize endophytic actinomycetes in different organs of Quercus serrata. For this purpose, actinomycete isolates were obtained from surface-sterilized tissues of both symptomless seedlings and aged trees of Q. serrata and rhizosphere soil of the sampled seedlings. Thirty-five isolates with the ability to sporulate, including 4 from leaves of the aged trees, 10, 6, and 15 from leaves, stems, and roots of the seedlings, respectively, and 8 soil-derived isolates, were selected and characterized. The 16S rDNA nucleotide sequence analyses revealed that all of them belonged to the genus Streptomyces. According to a neighbor-joining phylogenetic tree constructed based on the results, the isolates of plant origin were divided into three major clades with high bootstrap values of 98 or 99 %, whereas eight soil-derived isolates were located at different positions from those of the endophyte isolates. Moreover, two larger clades were formed, one of which contained isolates derived only from aboveground parts, while the other contained isolates from all of the organs. These results suggest that the endophytic streptomycetes in Q. serrata may differ in their habitat positions (i.e., either above- or belowground parts).  相似文献   

Downy mildew of busy lizzie caused by Plasmopara obducens in Japan     

Mamoru Satou  Kei Sugawara  Susumu Nagashima  Toshihide Tsukamoto  Yosuke Matsushita 《Journal of General Plant Pathology》2013,79(3):205-208

In June 2010 and March 2012, busy lizzie (Impatiens sultanii) plants in a glasshouse in Yamagata Prefecture and in nurseries in a plastic house in Shimane Prefecture, Japan were observed with yellowing of the adaxial surface of leaves or cotyledons and a white downy growth on the abaxial surface of leaves or cotyledons. We identified the causal pathogen as Plasmopara obducens based on the morphologies and rDNA-large subunit sequences. This new disease was named “downy mildew of busy lizzie” in Japan.  相似文献   

First report of a <Emphasis Type="Italic">Tomato chlorotic dwarf viroid</Emphasis> disease on tomato plants in Japan     

Yosuke Matsushita  Ayami Kanda  Tomio Usugi  Shinya Tsuda 《Journal of General Plant Pathology》2008,74(2):182-184

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