Multiple high-throughput analyses monitor the response of E. coli to perturbations     

Ishii N  Nakahigashi K  Baba T  Robert M  Soga T  Kanai A  Hirasawa T  Naba M  Hirai K  Hoque A  Ho PY  Kakazu Y  Sugawara K  Igarashi S  Harada S  Masuda T  Sugiyama N  Togashi T  Hasegawa M  Takai Y  Yugi K  Arakawa K  Iwata N  Toya Y  Nakayama Y  Nishioka T  Shimizu K  Mori H  Tomita M 《Science (New York, N.Y.)》2007,316(5824):593-597

Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.  相似文献   

Variability of Micro-elevation,Yield, and Protein Content within a Transplanted Paddy Field     

K.?ShojiEmail author  T.?Kawamura  H.?Horio  K.?Nakayama  N.?Kobayashi 《Precision Agriculture》2005,6(1):73-86

Due to the nature of waterlogged fields used for rice production, we hypothesized that micro-elevation (micro-relief, micro-topography, or differences in elevation) is an important factor for site-specific management within rice fields. A 0.5-ha transplanted and weed-free paddy field was selected as the observation site, where there was micro-elevation in a range of 100 mm within the field. Combine-monitored grain yield and the surveyed micro-elevation were compared at 96 locations in the field, and 60 hand-taken grain samples were analysed for protein content. Grain yield and protein content showed significant negative correlations with micro-elevation (r=-0.50^*** and -0.67^***, respectively), indicating that at lower elevations, grain yield increased gradually with protein content. Spatial variation in yield and protein content was attributed to availability of water and nutrient uptake at locations with different micro-elevation. Therefore, micro-elevation is expected to be one of the important factors for managing spatial variation in a small paddy field.  相似文献   

Cytological and microsatellite mapping of the genes determining liguleless phenotype in durum wheat     

N.?WatanabeEmail author  A.?Nakayama  T.?Ban 《Euphytica》2004,140(3):163-170

Liguleless phenotypes of wheat lack ligule and auricle structures on all leaves of the plant. Two recessive genes principally control the liguleless character in tetraploid wheat. The F₂ progenies of k17769 (liguleless mutant)/Triticum dicoccoides and k17769/T. dicoccum segregated in a 15:1 ratio, whereas the F₂ progenies of k17769/T. durum and k17769/T. turgidum segregated in a 3:1 ratio. A new gene, lg₃, was found on chromosome 2A. Segregation of F₂ progenies between k17769 and chromosome substitution lines for homoeologous group 2 chromosomes suggested that the liguleless genotype had occurred by mutation at the lg₃ locus on chromosome 2A, and then by mutation at the lg₁ locus on chromosome 2B, in the process of domestication of tetraploid wheat. The gene (lg₁) was linked to Tc₂ (11.9 cM), which determines phenol colour reaction of kernels, on the long arm of chromosome 2B. The distance of lg₁ to the centromere was found to be 60.4 cM, and microsatellite mapping established the gene order, centromere – Xgwm382 – Xgwm619 – Tc₂ – lg₁ on the long arm of chromosome 2B.  相似文献   

Analysis of Ferulic and p‐Coumaric Acids in Japanese Rice for Sake Brewing     

Katsumi Hashizume  Toshihiko Ito  Airi Nakayama  Masaki Okuda 《Cereal Chemistry》2014,91(1):45-49

Ferulic and p‐coumaric acids were analyzed in 50 rice (Oryza sativa L.) samples from 32 cultivars harvested in Japan. In brown rice, ferulic and p‐coumaric acid levels ranged from 309 to 607 mg/kg and from 49 to 100 mg/kg, respectively. In 70% polished rice, ferulic and p‐coumaric acid levels ranged from 27 to 103 mg/kg and from 0.4 to 3.5 mg/kg, respectively. Ratios of average phenolic acid levels in the 70% polished rice to the brown rice were 13.9% for ferulic acid and 1.9% for p‐coumaric acid. The ferulic acid level was highly correlated between brown and 70% polished rice (R = 0.815; P < 0.01), but there was no clear correlation for p‐coumaric acid. Phenolic acid levels in the 70% polished rice did not show any clear correlations between the analytical index measurements for sake brewing suitability (weight of 1,000 grains, water absorption, digestibility, crude protein, and potassium content). Phenolic acid levels in the 70% polished rice directly affected levels in the rice koji enzyme digest. The results indicated that phenolic acid levels in sake were affected by the levels in ingredient rice grains, which may then influence the sensory quality of sake.  相似文献   

Maternal and genetic effects on individual variation during early development in Japanese flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>     

Yukinori Shimada  Takahito Shikano  Naoto Murakami  Tatsuo Tsuzaki  Tadahisa Seikai 《Fisheries Science》2007,73(2):244-249

ABSTRACT:   To investigate the changes in maternal effects during early development in Japanese flounder Paralichthys olivaceus , four-by-four factorial mating by artificial fertilization was performed to produce 16 families. Larvae and juveniles of each family were reared under common environmental conditions until completion of metamorphosis. The magnitude of maternal effects was estimated as the differences between sire and dam variance. Maternal effects were highest at hatching, and then disappeared at 30 days after hatching. During early development, larval size at hatching was largely affected by egg size, but this effect decreased as larvae grew. Estimated mean heritability during early development was nearly constant (0.14 ± 0.06 for total length and 0.10 ± 0.05 for body depth). Therefore, it is suggested that early development in Japanese flounder is affected by both maternal effect and genetic factors.  相似文献   

An H5N1 highly pathogenic avian influenza virus that invaded Japan through waterfowl migration     

Kajihara M  Matsuno K  Simulundu E  Muramatsu M  Noyori O  Manzoor R  Nakayama E  Igarashi M  Tomabechi D  Yoshida R  Okamatsu M  Sakoda Y  Ito K  Kida H  Takada A 《The Japanese journal of veterinary research》2011,59(2-3):89-100

In 2010, an H5N1 highly pathogenic avian influenza virus (HPAIV) was isolated from feces of apparently healthy ducks migrating southward in Hokkaido, the northernmost prefecture of Japan. The H5N1 HPAIVs were subsequently detected in domestic and wild birds at multiple sites corresponding to the flyway of the waterfowl having stopovers in the Japanese archipelago. The Hokkaido isolate was genetically nearly identical to H5N1 HPAIVs isolated from swans in the spring of 2009 and 2010 in Mongolia, but less pathogenic in experimentally infected ducks than the 2009 Mongolian isolate. These findings suggest that H5N1 HPAIVs with relatively mild pathogenicity might be selected and harbored in the waterfowl population during the 2009-2010 migration seasons. Our data provide "early warning" signals for preparedness against the unprecedented situation in which the waterfowl reservoirs serve as perpetual sources and disseminators of HPAIVs.  相似文献   

Multiplex PCR and Genescan analysis to detect immunoglobulin heavy chain gene rearrangement in feline B-cell neoplasms   总被引：1，自引：0，他引：1  

Mochizuki H  Nakamura K  Sato H  Goto-Koshino Y  Sato M  Takahashi M  Fujino Y  Ohno K  Uchida K  Nakayama H  Tsujimoto H 《Veterinary immunology and immunopathology》2011,143(1-2):38-45

Lymphoid neoplasms are usually diagnosed on the basis of cytological and histopathological findings. However, in some cases, discrimination of lymphoid neoplasms from reactive lymphoid proliferation is difficult. PCR amplification of complementarity-determining region 3 (CDR3) of the immunoglobulin heavy-chain variable region (IGHV) gene can be used to assess clonality of B-cell populations as a supportive diagnostic tool for B-cell neoplasms. Because of the sequence variation and possible somatic hypermutation of the IGHV gene, sensitivity of the PCR-based assay to detect clonal IGHV gene rearrangement largely depends on the sequences and numbers of primer sets. Prior to the development of an efficient assay, we cloned and sequenced 97 IGHV complementary DNAs (48 IGHV-1 and 49 IGHV-3 clones) from normal cat spleens. On the basis of these sequences, we designed 6 forward primers at the variable region and 5 reverse primers at the joining region. Using each of 6 forward primers and a mixture of 5 reverse primers, we amplified CDR3 of IGHV genes and analyzed the PCR products by conventional PAGE and Genescan analyses using fluorescence-labeled primers. Twenty-six feline B-cell neoplasms diagnosed by histopathological and immunohistochemical examinations were subjected to the newly developed analysis of IGHV gene rearrangement. Clonal IGHV gene rearrangement was detected in 22 of 26 (84%) samples by both PAGE and Genescan analyses. To reduce the number of PCR reactions, we constructed a multiplex PCR analysis system using a mixture of IGHV-1- and IGHV-3-specific primers as forward primers and a mixture of 5 joining region reverse primers. Results of the multiplex PCR were 100% concordant with those obtained by each of the singleplex PCRs. The multiplex PCR-based assay and Genescan analysis developed in the present study would be useful and practical tools to detect clonal IGHV gene rearrangement in feline B-cell neoplasms.  相似文献   

Broth from canned clams is suitable for use as an excellent source of free vitamin B12     

Ueta K  Takenaka S  Yabuta Y  Watanabe F 《Journal of agricultural and food chemistry》2011,59(22):12054-12058

Vitamin B(12) was assayed and characterized in the broth of canned clams (boiled plain). The broth contained considerable amounts of vitamin B(12) (2.7-14.1 μg/100 g, 1.3-6.7 μg/can). HPLC and LC/ESI-MS/MS chromatograms demonstrated that the clam broth contained true vitamin B(12). Gel filtration experiments indicated that most (72%) of the vitamin B(12) found in the broth was recovered in free vitamin B(12) fractions. These results indicate that the clam broth would be suitable for use an excellent source of free vitamin B(12) for elderly persons with food-bound vitamin B(12) malabsorption.  相似文献   

Radiofrequency radiation at 40 kHz induces hepatic injury in Long-Evans Cinnamon (LEC) rats, an animal model for human Wilson disease     

Sakai H  Horiguchi N  Endoh D  Nakayama K  Hayashi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):299-304

In the present study, we examined effects of radiofrequency (RF) radiation at 40 kHz on hepatic injury in Long-Evans Cinnamon (LEC) rats, an animal model for human Wilson disease, which is a heritable disease of copper metabolism in the liver. The activities of ALT and AST in serum of LEC rats exposed to RF radiation for 2 weeks were approximately 3.8-fold and 2-fold higher than those in serum of sham-exposed rats, respectively. Although there were no significant differences in hepatic copper contents between LEC rats exposed to RF radiation for 2 weeks and sham-exposed rats, copper contents in the kidney and serum of exposed LEC rats were approximately 4.2-fold and 12.9-fold higher than those in sham-exposed rats, respectively. Relative O??-scavenging activities in the S-100 fraction of the liver of LEC rats exposed to RF radiation for 2 weeks were 1.6-fold higher than those in sham-exposed rats. No significant differences were observed in activities of AST and ALT in serum and relative O??-scavenging activity in the S-100 fraction of the liver of normal control WKAH rats that were sham-exposed and exposed to RF radiation. No significant differences were observed in copper contents in the liver, kidney and serum of WKAH rats that were sham-exposed and exposed to RF radiation for 2 weeks. The results show that RF radiation at 40 kHz induced hepatic injury in LEC rats.  相似文献   

Irrigated guayule — Production and water use relationships     

D.A. Bucks  F.S. Nakayama  O.F. French  W.W. Legard  W.L. Alexander 《Agricultural Water Management》1985,10(1):95-102

Interest in developing the guayule plant (Parthenium argentatum Gray) as a source of natural rubber has increased in the United States and other countries during the last decade. A comprehensive study was initiated to obtain information on the production and water-use relationships for guayule. Water-use efficiencies for three cultivars after 2 years ranged from 0.70 to 0.85 kg/m³ for dry matter, 0.045 to 0.055 kg/m³ for resin, and 0.03 to 0.04 kg/m³ for rubber production. The dry matter, resin, and rubber yield were shown to be linearly related to evapotranspiration. Large quantities of irrigation water were needed to get high yields in this short period of time. Relating yield and crop stress on a seasonal basis indicated that guayule grown in an arid environment was more sensitive to water stress in the latter than the first half of the year. Although the drought tolerance characteristics of the guayule crop permits flexibility in irrigation scheduling, the irrigation water requirement can be high in order to increase yields and shorten the harvest time to 3 years or less.  相似文献