哈茨木霉天冬氨酸蛋白酶基因SA76的3＇RACE扩增和序列分析     

刘燕  杨谦 《林业研究》2007,18(2):139-143

由EST获得全长cDNA对于结构基因组学和功能基因组学都是至关重要的,cDNA末端快速扩增技术RACE是该领域中的重要研究方法.利用BD SMART RACE技术扩增编码分泌天冬氨酸蛋白酶SA76基因的3＇末端,将其与哈茨木霉cDNA文库中的SA76基因的EST序列进行序列拼接,获得2019bp的全长cDNA序列,其开放读码框长1593bp,5＇非编码区266bp,3＇非编码区201bp,编码530个氨基酸,有信号肽.哈茨木霉天冬氨酸蛋白酶基因与玉蜀黍赤霉、粗糙脉孢菌、球毛壳菌天冬氨酸蛋白酶基因的同源性分别为53%, 37%, 36%.利用BD SMART RACE技术首次从哈茨木霉中克隆天冬氨酸蛋白酶基因,为验证SA76基因的功能奠定基础,为进一步研究蛋白酶的作用机制及生物防治功能提供依据.  相似文献   

Socio-economic significance of reed forests in a rural community: A case study from the greater Sylhet Region of Bangladesh   总被引：1，自引：0，他引：1  

Qumruzzaman Chowdhury  A. Z. M. Manzoor Rashid  Masuduzzaman Afrad 《Small-Scale Forestry》2004,3(1):121-130

The reed forests that are the subject of this study are scattered over five thanas of the Sunamganj and Sylhet Districts of the Sylhet Division of Bangladesh. Their total area is 23,590 ha and they have great ecological, economic, commercial and socio-economic importance due to the diversified resources they supply. However, the forests are commonly encroached on by local people who are perceived to be seriously depleting the resource. This paper deals with the socio-economic status of the people residing near these reed forests, and examines their dependency on the resources provided by the reed forests. A socio-economic survey, participatory rural appraisal and interviews were used to obtain baseline data of the reed forest resource and the local communities. The study reveals that the communities in the study region have higher income than other areas of Bangladesh. Most survey respondents have other occupations in addition to farming. The literacy rate is 28.8%. Among the illiterate, 68% engage in collecting reeds. Although the Forest Department has a management plan for sustainable use of the reed forests, encroachers receive backing from political leaders and local elites, so their eviction is difficult. In order to achieve long-term productivity and sustainability from the reed forests, this study recommends a strategy of developing an integrated joint management plan between the Forest Department and the local people.  相似文献   

The use of compensatory and non-compensatory multi-criteria analysis for small-scale forestry     

Ian Jeffreys 《Small-Scale Forestry》2004,3(1):99-117

Small-scale farm forestry has the potential to offer many benefits both to landholders and the wider community. As with all changes in land-use practices, there are associated benefits and costs and these are not uniformly distributed. They have varying impacts on the different values, aspirations, goals and objectives that exist within the community. Furthermore, the community does not consider these values, aspirations, goals and objectives of equal importance. The degree of concern can vary from minor to high and overriding all other considerations. When evaluating farm forestry options it is necessary to address all of these concerns. This paper examines the combined use compensatory and non-compensatory multi-criteria analyses to evaluate forestry options, in a case study for the Darling Downs region of Queensland, Australia. These aggregation techniques are found to be highly complementary and together provide a comprehensive analysis. The compensatory technique provides a sound measure of overall performance of a forestry system, whereas the non-compensatory technique alerts decision-makers to presence of particularly poor performance with respect to individual criteria. The compensatory technique used is simple and understandable even for those with non-mathematical backgrounds. This analysis can identify and aid communication of the relative benefits and costs, and trade-offs, between economic, environmental and social considerations.  相似文献   

Predicting moisture content and density distribution of Scots pine by microwave scanning of sawn timber     

Jan Johansson  Olle Hagman  Bengt-Arne Fjellner 《Journal of Wood Science》2003,49(4):312-316

This study was carried out to investigate the possibility of calibrating a prediction model for the moisture content and density distribution of Scots pine (Pinus sylvestris) using microwave sensors. The material was initially of green moisture content and was thereafter dried in several steps to zero moisture content. At each step, all the pieces were weighed, scanned with a microwave sensor (Satimo 9,4GHz), and computed tomography (CT)-scanned with a medical CT scanner (Siemens Somatom AR.T.). The output variables from the microwave sensor were used as predictors, and CT images that correlated with known moisture content were used as response variables. Multivariate models to predict average moisture content and density were calibrated using the partial least squares (PLS) regression. The models for average moisture content and density were applied at the pixel level, and the distribution was visualized. The results show that it is possible to predict both moisture content distribution and density distribution with high accuracy using microwave sensors.  相似文献   

多效抗旱驱鼠剂室内促长效果研究   总被引：1，自引：0，他引：1  

杨学军  韩崇选  王明春  杨清娥  李军 《陕西林业科技》2002,(2):14-18

室内用多效抗旱驱鼠剂对小麦、黄豆、油松、侧柏种子进行浸种、拌种处理。结果表明，300x的林用型和200x的农用型RPA对侧柏种子浸种效果最好，30x的林用型和80x的农用型RPA拌种效果最好，经检验分析，差异显著；500x的林用型和600x的农用型RPA对油松促长效更为明显，发芽率比对照提高25．2％－27．0％，平均高生长增加2．1－2．8cm，拌种型的两种药剂6个浓度对油松的发芽及生长影响比较明显，30x的林用型和40x的农用型可以使油松发芽率提高27．7％－29．8％，苗高增长0．6－1．3cm；对黄豆的发芽率提高12．0％－17．0％、13．8％－23．2％；侧根数量增加0．2－2．6、0．3－1．6条，平均苗高及生物量也有不同程度的提高；浸种型的RPA使小麦发芽率提高13．3％－23．3％，苗高增长5．1－7．5cm，根长增加0．6－1．8cm；拌种型RPA发芽率提高9．3％－22．6％，苗高增长4．7－6．6cm，根长增加0．4－1．6cm。  相似文献   

<Emphasis Type="Italic">Pinus halepensis</Emphasis> somatic embryogenesis is affected by the physical and chemical conditions at the initial stages of the process     

Catia Pereira  Itziar Aurora Montalbán  Olatz García-Mendiguren  Tomás Goicoa  Maria Dolores Ugarte  Sandra Correia  Jorge Manuel Canhoto  Paloma Moncaleán 《Journal of Forest Research》2016,21(3):143-150

Pinus halepensis has been described as a drought-tolerant species with high plasticity to growth in different environments. Its eco-physiological characteristics could facilitate the use of this species in large afforestations in the future scenery of climate change. Somatic embryogenesis is a biotechnological tool with potential for large-scale clonal propagation. In order to establish an improved regeneration protocol for Pinus halepensis, the effects of different temperatures (18, 23, and 28 °C) and water availability conditions (2, 3, and 4 g L^?1 Gelrite^®), during initiation of embryonal masses on the rate of initiation, proliferation, maturation, and the number of embryos developed, were evaluated. It was found that environmental conditions during the initiation stage of Pinus halepensis somatic embryogenesis influence the success of initiation and proliferation. In contrast, there was no effect of these conditions on the maturation rates and the number of somatic embryos. Somatic embryos were obtained in all treatments tested, indicating that plants can be produced from extreme conditions of induction, such as high temperatures (28 °C) and low water availability conditions (4 g L^?1).  相似文献   

Establishment of a high-frequency regeneration system in <Emphasis Type="Italic">Cerasus humilis</Emphasis>, an important economic shrub     

Rui Fang Wang  Feng Lan Huang  Jian Zhang  Qiu Yan Zhang  Li Na Sun  Xing Shun Song 《Journal of Forest Research》2016,21(5):244-250

Cerasus humilis is a species of small, perennial, drought-resistant and multipurpose deciduous shrub grown in arid and semi-arid conditions in northern China. In this study, an efficient protocol for the rapid micropropagation of C. humilis has been standardized using stem and/or leaf explants. Direct multiple shoot induction was observed when the stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. The highest shoot induction was obtained when stem explants from adult trees were cultured on MS medium supplemented with 2.0 mg L^?1 6-benzyladenine (6-BA) and 0.9 mg L^?1 α-naphthaleneacetic acid (NAA). The leaf and stem explants cultured on MS medium with 1.0 mg L^?1 6-BA and 0.6 mg L^?1 NAA, and 0.5 mg L^?1 6-BA and 0.8 mg L^?1 NAA, respectively, produced the highest induction frequency of callus. Maximum proliferation of callus was observed on MS medium containing a combination of 0.5 mg L^?1 6-BA with 0.6 mg L^?1 2,4-dichlorophenoxyacetic acid (2,4-d). Optimal shoots differentiated from callus were obtained on MS medium supplemented with 5.0mg L^?1 6-BA and 0.9 mg L^?1 NAA. In vitro rooting was achieved on half-strength (1/2) MS medium containing 0.5 mg L^?1 NAA. Rooted plantlets were hardened under control conditions and successfully acclimatized under field conditions.  相似文献   

Chemical composition,antioxidant and antibacterial activities of extracts from <Emphasis Type="Italic">Schinus molle</Emphasis> wood branch growing in Egypt     

Mohamed Z. M. Salem  Mohamed Z. Zayed  Hayssam M. Ali  Mamoun S. M. Abd El-Kareem 《Journal of Wood Science》2016,62(6):548-561

In the present work, for the first time, the chemical components of essential oils (EOs) and extracts from wood branch (WB) resulted from the tree pruning wastes of Schinus molle L. grown in Egypt were evaluated for their antioxidant and antibacterial activities. EOs, methanol (ME), dichloromethane (DCME) and water (WE) extracts as antioxidant and antibacterial activities were measured. Total phenolic and flavonoid contents as well as analysis of extracts by gas chromatography–mass spectrometry (GC–MS) were reported. The major components in EOs were α-elemol, β-pinene, and α-phellandrene, in ME were 6-(4-chlorophenyl)-3-cyano-4-(N-benzylpiperazino)-2H-pyran-2-one, and 2-naphthalene methanol, decahydro-α,α,4a-trimethyl-8-methylene, in DCME were 12-methyl-E,E-3,13-octadecadien-1-ol, and 1,2-benzenedicarboxylic acid, dioctyl ester, and in WE were β-eudesmol, and (Z,Z,Z)-9,12,15-octadecatrienoic acid, 2,3-dihydroxypropyl ester. The highest total antioxidant activity was found with EOs (90 ± 1.23 %) and WE (86.30 ± 1.40 %). The lowest IC₅₀ values of 13.11 ± 3.00, and 12.66 ± 2.15 μg/mL were found with WE and EOs, respectively. EOs and WE were observed to have good antibacterial activity against Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Sarcina lutea, Pseudomonas aeruginosa, and Micrococcus luteus. In conclusion, the Schinus molle L. WB EOs and extracts might, indeed, be used as a potential source for pharmaceutical or food industries.  相似文献   

The Level II aggregated forest soil condition database links soil physicochemical and hydraulic properties with long-term observations of forest condition in Europe     

Stefan Fleck  Nathalie Cools  Bruno De Vos  Henning Meesenburg  Richard Fischer 《Annals of Forest Science》2016,73(4):945-957

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Analysis of sawn lumber production from logging residues of branchwood of <Emphasis Type="Italic">Aningeria robusta</Emphasis> and <Emphasis Type="Italic">Terminalia ivorensis</Emphasis>     

Reynolds Okai  Okyere Boateng 《European Journal of Forest Research》2007,126(3):385-390

One way of meeting the supply demands of the wood industry is through the reduction of waste in timber processing. It has been estimated in Ghana that for every tree felled, nearly 50% of the tree volume is left in the forest in the form of branches, crownwood, and stumps. In this study, the potential of utilizing branchwood as raw material from logging residues to help meet the timber demands of the downstream processing sector was analyzed experimentally. The sawing characteristics of the branchwood of Aningeria robusta and Terminalia ivorensis with diameters in the range 10–25 cm using live sawing and cant sawing methods were determined using a randomized complete block design, 2 × 4 factorial with three replications. Experimental results indicate that, the relative lumber value yields for first and second grade boards of A. robusta and T. ivorensis branches were 25 and 20%, respectively, while the green lumber volume yields were 40 and 32%, respectively. A high proportion of sapwood and the occurrence of washboarding in T. ivorensis contributed to that species’ relatively low lumber value and volume yield. The experimental results further indicate that the sawing technique used had no significant influence on lumber yields. However, at 5% level of significance, significant differences in lumber yield existed between some of the diameter classes.  相似文献