薯蓣内生真菌的分离及其抑制植物病原真菌的活性   总被引：2，自引：1，他引：1  

周生亮  陈双林  房兴堂  蒋虹  魏志文 《江苏农业科学》2007,(4):64-67

从盾叶薯蓣(Dioscorea zingiberensis C.H.Wright)各部位共分离到50株内生真菌,初步鉴定有19株分属于曲霉属(Aspergillus Micheli ex Fr.)、须壳霉属(Pyrenochaetade Not)、刺孢壳属(Chaetomella Fuckel)、侧孢霉属(Sporotrichum Lk.ex Fr.)、棒束梗霉属(Isaria Pers.ex Fr.)、假黑粉霉属(Coniosporium Lk.ex Fr.)和简梗孢霉属(Chromosporium Corda),其余菌株因不产孢而暂未鉴定。利用生长速率法和孢子萌发法检测到分离于根茎的EDT3、EDT4、EDT5和分离于种子的EDS1、EDS3、EDS4等6株菌株的发酵液对棉花黄萎病菌(Verticillium dahliaeKleb.)等6种植物病原真菌的菌丝生长有抑制作用,其中EDS3和EDS4发酵液的混合液E-y对这6种植物病原真菌的菌丝生长和孢子萌发均有较强的抑制作用。  相似文献   

石灰质量调查及添加量对糙皮侧耳熟料栽培的影响     

刘天海  苗人云  陈春秀  刘理旭  周洁  彭卫红  黄忠乾  谭昊 《中国农学通报》2021,37(6):44-48

石灰是食药用菌生产的重要辅料,研究旨在对当前食用菌栽培过程中使用的石灰辅料进行质量摸底,同时研究石灰在糙皮侧耳栽培中的最适添加量,达到精准指导糙皮侧耳和其他食用菌生产的目的.采用消化石灰物相分析法,对8份不同来源石灰的Ca(OH)2、CaO、CaCO3和MgO含量进行检测.同时选用7号石灰按不同比例添加至玉米芯培养料,...  相似文献   

鲤鱼血清蛋白,血红蛋白,LDH和EST同工酶的电泳分析     

叶元土  陈小英 《水产养殖》1992,(5):12-14

采用聚丙烯酰胺凝胶电泳技术,分析了鲤鱼性成熟期雌雄个体的血清蛋白、血清脂蛋白和血红蛋白,结果是雌雄个体血清蛋白电泳图谱有差异,雌性(14条)比雄性(13条)多1条蛋白带;血清脂蛋白和血红蛋白无性别差异。同时,测定了鲤鱼血清、肝胰脏、心脏、背部肌肉和眼晶体的乳酸脱氢酶(LDH)同工酶和酯酶(EST)同工酶,LDH具有组织特异性,除在肝胰脏分离出9条酶带外,在血清中也分离出8条酶带,表明有LDH—c基因的作用,EST同工酶表现出明显的多态性,表明在鲤鱼各组织中有多个基因座位起作用。  相似文献   

贝类受精细胞学的研究现状     

王洲熊良伟  王帅兵殷洁馨 《齐鲁渔业》2006,23(4):13-16

从成熟卵子的核相与体外促熟,精子的顶体反应,受精膜的形成,极体的释放与雌雄原核的形成以及多精入卵方面综述了贝类受精细胞学的研究成果,并展望了这一领域的研究和应用前景。  相似文献   

Effect of dietary vitamin B6 supplementation on growth and intestinal microflora of juvenile golden pompano (Trachinotus ovatus)     

Qianqian Huang  Heizhao Lin  Ruixuan Wang  Zhong Huang  Chuanpeng Zhou  Wei Yu  Pengwei Xun  Lianjie Tan  Yun Wang  Jun Wang 《Aquaculture Research》2019,50(9):2359-2370

In order to investigate the dietary vitamin B₆ (VB₆) requirement for juvenile golden pompano, an experiment of six different diets with six dietary VB₆ levels (0, 3.75, 7.47, 10.6, 13.7 and 18.5 mg/kg) was conducted. The results indicated that the content of dietary VB₆ significantly increased weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR; p < 0.05). Golden pompano fed diet containing 7.47 mg/kg VB₆ achieved the maximum ALT, AST, GPx, hepatic VB₆ concentrations as well as the minimum MDA, however, the ALT, AST, GPx and hepatic VB₆ concentrations decreased while MDA increased with a further increase in dieatry VB₆. The highest values of GR and POD occurred at the 10.6 and 13.7 mg/kg dietary VB₆ (p < 0.05) respectively. A diet supplemented with 7.47 mg/kg VB₆ increased intestinal Na⁺, K⁺‐ATPase, Chymotrypsin, γ‐glutamyl transpeptidase, creatine kinase and amylase activities (p < 0.05). The relative abundance of Tenericutes and Bacteroidetes decreased while Proteobacteria and Firmicutes improved with an increase in dietary VB₆ levels up to 7.47 mg/kg. Quadratic regression analysis on WGR, hepatic VB₆ concentrations, AST and ALT indicated that the optimum dietary VB₆ levels for juvenile golden pompano were 8.84–9.28 mg/kg.  相似文献   

中国对虾亲虾的能量代谢研究   总被引：16，自引：4，他引：16  

周洪琪 《水产学报》1990,14(2):114-119

本试验测定了中国对虾亲虾在禁食和静息状态下的耗氧率、二氧化碳排出率和氨氮排泄率,研究了它们的呼吸商、氧氮比、能源的化学本质及其消耗量之比。结果表明,体重为85.1±14.8克的亲虾,在水温19～22℃时的耗氧率为0.25±0.08毫克/克·小时,二氧化碳排出率为0.28±0.09毫克/克·小时,氨氮排泄率为8.86±3.53微克/克·小时;亲虾以蛋白质、脂肪和碳水化合物的混台物为能源,其中以蛋白质为主,脂肪次之,碳水化合物的利用最少;亲虾的耗氧率(Y)与体重(W)呈负相关,Y=1.89W~(-1.3),而与性腺发育的影响则相反,性腺发育好的亲虾代谢率高,性腺发育差的亲虾代谢率低。  相似文献   

Effects of lysine and methionine supplementation on growth,body composition and digestive function of grass carp (Ctenopharyngodon idella) fed plant protein diets using high‐level canola meal          下载免费PDF全文

J. Jiang  D. Shi  X.‐Q. Zhou  L. Feng  Y. Liu  W.‐D. Jiang  P. Wu  L. Tang  Y. Wang  Y. Zhao 《Aquaculture Nutrition》2016,22(5):1126-1133

A 76‐day feeding trial was carried out to evaluate the effects of Lysine and Methionine supplementation on growth and digestive capacity of grass carp (Ctenopharyngodon idella) fed plant protein diets using high‐level canola meal (CM). Fish with initial average weight 103.9 ± 0.6 g were fed three extruded diets. Fish meal (FM) diet was formulated as the normal control with 40 g kg^?1 FM and 300 g kg^?1 CM; CM diet was prepared by replacing all FM with CM (total 340 g kg^?1) without Lys or Met supplementation; CM supplement (CMS) diet was similar to CM diet but was supplemented with essential amino acids (EAA) to ensure the levels of Lys and Met similar to those in the FM diet. Feed intake, feed efficiency and specific growth rate of the grass carp fed CMS and FM diets were similar (P > 0.05), but higher than those of the grass carp fed CM diet (P < 0.05). The hepatosomatic index, relative gut length, intestosomatic index and intestinal folds height were significantly improved in fish fed FM and CMS diets as compared to CM diet (P < 0.05). Lower activities of trypsin, lipase and amylase in hepatopancreas were observed in fish fed CM diet (P < 0.05). Three hundred and forty gram per kilogram CM without Lys or Met supplementation significantly decreased trypsin, lipase and amylase mRNA levels in hepatopancreas (P < 0.05). These results indicated that the high supply of CM (340 g kg^?1) in plant protein (200 g kg^?1 soybean meal and 100 g kg^?1 cottonseed meal) diets decreased digestive ability through decreasing digestive enzyme activities and enzyme gene's expressions of grass carp, and these side effects can be reversed by supplementing Lys and Met. Therefore, CM could be high level used in a plant protein blend‐based extruded diet for grass carp as long as EAA were supplemented.  相似文献   

中国蛤蜊鳃的光镜与扫描电镜观察   总被引：4，自引：0，他引：4  

崔龙波  孔俊  周雪莹 《海洋水产研究》2005,26(4):60-63

用光镜和扫描电镜观察了中国蛤蜊鳃的组织学和表面结构。所有鳃丝的组织结构均相同。鳃丝上皮按结构与功能可区分为4个区带：前纤毛柱状细胞区、侧纤毛柱状细胞区、粘液细胞区和扁平细胞区。前纤毛和侧纤毛分别与食物的运送和呼吸水流的产生有关。扁平细胞为呼吸上皮，其结构有助于气体交换。相邻鳃丝通过成排的、含有血管的丝间连接连系。  相似文献   

草鱼出血病的电镜观察   总被引：3，自引：0，他引：3  

郑德崇  黄琪琰  赵立勤  周孝康 《水产学报》1991,15(4):317-321

草鱼出血病是由呼肠孤病毒感染所致的急性传染病，由于病鱼小血管受损引起全身性出血，并造成内脏器官和体壁肌肉组织局灶性病变。电镜观察表明病变细胞内各种细胞器的数量较正常细胞显著减少，形态结构也遭损坏。质膜的三层结构模糊不清，及至解体消失。与机体代谢密切相关的粗面内质网、滑面内质网、线粒体和高尔基复合体的数量减少及结构受损，使蛋白质、糖类和脂质合成及分解等一系列细胞代谢活动不能正常进行，加速了病鱼死亡。  相似文献   

Soy protein concentrate as a substitute for fish meal in diets for juvenile Acanthopagrus schlegelii: effects on growth,phosphorus discharge and digestive enzyme activity          下载免费PDF全文

Hameeda Kalhoro  Jing Zhou  Ying Hua  Wing‐Keong Ng  Lian Ye  Jinzhi Zhang  Qingjun Shao 《Aquaculture Research》2018,49(5):1896-1906

The current high demand and cost of fish meal (FM) necessities the evaluation of alternative plant protein ingredients in diets of farmed marine fish. A 56‐day feeding trial was performed to study the effects of replacement of FM with soy protein concentrate (SPC) in diets of Acanthopagrus schlegelii. Diets were prepared at levels of 0%, 30%, 47.5%, 65%, 82.5% and 100% SPC, respectively, replacing FM. The results indicated no significant differences (p > .05) in % weight gain (WG) and specific growth rate of fish fed S30% to S82.5% diets compared with the control diet while, further inclusion at 100% significantly depressed growth performance. SPC inclusion and phytase supplementation significantly affected the phosphorous discharge (P‐load) showing lowest value (3.83 ± 0.53 g/kg WG) in S100% compared to control (14.79 ± 0.37 g/kg WG) and in fish fed S30% diet (13.24 ± 0.89 g/kg WG) (p < .05). The results of this study showed that FM could be substituted up to 82.5% by SPC in the diet of Acanthopagrus schlegelii fingerlings (5.53 ± 0.12 g) without any adverse effects. Phytase supplementation SPC based diets could be effective in reducing the phosphorus load in the aquatic culture environment.  相似文献