乌蔹梅的组织培养及快速繁殖研究   总被引：1，自引：0，他引：1  

杨威  于颖  王艳娇  徐娜  姜长阳 《湖南农业科学》2010,(9)

采用生长旺盛的乌蔹梅茎段为培养材料,进行芽的生长与分化,试管苗生根、继代、留茬继代、移栽、扦插和移植的研究.结果证明:1/2 MS+IAA 0.2mg/L+NAA0.1mg/L是芽生长培养的理想培养基;MS+BA 0.4 mg/L+NAA 0.1 mg/L是乌蔹莓生长芽分化增殖的理想培养基;1/2 MS+IAA 0.3mg/L是乌蔹莓试管莆生根培养的理想培养基;生根试管苗可以连续进行3次留茬继代培养,平均每代的繁殖系数为3.1;在温室的苗床上试管苗移栽和扦插成活率在90%左右;移栽和扦插成活的试管苗移植到花坛上的成活率近100%;移植的试管苗当年可以正常开花结实,且生长旺盛,根系发达.  相似文献   

冷水胁迫对云南粳稻育成品种农艺性状的影响^*     

赵国珍  YANG Sea-jun  YEA Jong-doo  袁平荣  朱振华  苏振喜  戴陆园  《云南农业大学学报(自然科学版)》2010,25(2):158-166

 以11个云南粳稻育成品种为试验材料,研究冷水胁迫对水稻农艺性状的影响以及品种耐冷性与农艺性状冷水反应的关系。结果表明,农艺性状的冷水反应不仅与品种有关,也与具体的农艺性状有关。冷水胁迫使株高、穗实粒数、结实率、单株产量和着粒密度极显著降低（P＜0.01）,穗长和剑叶长极显著缩短（P＜0.01）,剑叶宽度极显著变窄（P＜0.01）,剑叶与穗下节间的夹角极显著变小（P＜0.01）,一次和二次枝梗数极显著减少（P＜0.01）,有效穗极显著增加（P＜0.01）,而千粒重不因冷水胁迫发生明显变化（P＞0.05）。相关分析表明,水稻成熟期综合耐冷性与结实率呈极显著负相关（P＜0.01）,而结实率又与株高和单株产量冷水反应呈显著正相关（P＜0.05）。综合考虑供试品种的耐冷性,云粳19号、云粳20号、云粳优1号和云粳优15号耐冷性较强,可作育种亲本利用,以提高水稻品种的耐冷性。  相似文献   

对黏虫具有杀虫活性的Bt蛋白筛选及分析   总被引：1，自引：0，他引：1  

杨素娟  黄闽忠  李艳秋  周子珊  江幸福  高继国  程云霞  张杰 《植物保护》2016,42(3):30-35

为筛选对黏虫[Mythimna separata(Walker)]具有毒杀作用的苏云金芽胞杆菌杀虫蛋白,本研究提取Cry1类、Cry2类、Cry9类及Vip3A类等11种蛋白,通过人工饲料喂毒方法对黏虫进行生物活性测定。结果表明,Cry1Ac、Cry1Ab、Cry2Ab、Cry1Be及Cry1Bb蛋白对黏虫具有杀虫活性,其致死中浓度依次为5.09、17.71、26.75、27.42及43.93μg/g;Cry9Aa、Cry9Eb、Cry9Ee蛋白对黏虫生长具有抑制作用,其浓度为10μg/g时的体重抑制率分别为78.4%、79.0%、86.9%,浓度为100μg/g时的体重抑制率分别为92.8%、95.7%、96.7%;Cry1Ba、Cry1Ca和Vip3Aa蛋白对黏虫无显著活性。本研究为黏虫的生物防治奠定了基础,并为抗虫转基因作物的研究提供优良的候选基因。  相似文献   

水平转筒内大豆颗粒随机运动与混合特性模拟   总被引：2，自引：0，他引：2  

王瑞芳  李占勇  窦如彪  郭建忠 《农业机械学报》2013,44(6):93-99

基于离散单元法,利用EDEM软件对水平转筒内大豆颗粒运动的随机性与混合特性进行了模拟研究,分析了转筒转速与装载量对颗粒随机运动及混合特性的影响.结果表明:转筒转速的提高有利于改善颗粒的随机运动过程,而且颗粒的混合效果增强,混合速度加快.装载量的影响相反,随着装载量的增大,颗粒在转筒径向方向的随机运动虽有增大,但堆积现象比较明显,沿转筒轴向方向的随机运动明显降低,颗粒的混合速度变慢,混合效果变差.  相似文献   

电磁执行器直接驱动的AMT换挡机构     

李波  常思勤  林树森 《农业机械学报》2013,44(10):29-35

为提高机械式自动变速器（AMT）换挡品质并缩短换挡过程动力中断时间,提出了一种电磁直线执行器直接驱动的AMT换挡机构。换挡机构运动总质量为1.37 kg,较传统全电式AMT换挡机构总质量（约1.78 kg）降低23%。对换挡过程进行了分段研究,并在换挡过程数学模型和AMT试验台架基础上,验证了设计方案的可行性。换挡机构换挡过程中无选挡操作,可实现挡位切换时退、进挡同时进行的功能,转速差为500 r/min、转动惯量为0.03 kg·m2,换挡时3挡至4挡的换挡时间约为120 ms,提升了AMT的换挡品质,且仍有进一步提高的余地。研究表明,电磁直线执行器直接驱动的AMT换挡机构可实现自动换挡功能。  相似文献   

数字图书馆与知识产权问题的研究     

孙伟宏  刘绪真  庄丽华 《农业环境科学学报》2008,(3)

从数字图书馆与知识产权的概念、数字图书馆的建设和数字图书馆的合理使用等 几个方面对数字图书馆建设中的知识产权保护问题进行了探讨与分析。

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蛋白质组学研究现状及我国研究实力     

张惠荣  姜玉梅  张威 《农业环境科学学报》2008,(4)

以SCIE数据库为资料来源,统计1998-2005年蛋白质组学研究 论文的高产国家、机构、发表论文期刊及文献主题等相关指标,揭示蛋白质组学的研究现状 ,并对论文数量和影响力进行比较,以衡量我国在蛋白质组领域的研究实力。

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Pile Foundation Analysis Using a Boundary Element Method     

AI Zhi yong  LIU Feng 《保鲜与加工》2008,(4):73-77

Taking the extended Mindlin solution of a vertical point load acting in the interior of a multi layered elastic medium as the basic solution, an indirect boundary element method (BEM) was adopted to analyze pile foundations embedded in a multi layered elastic soil. The numerical analysis was carried out by the compiled program for a pile foundation in an homogeneous soil and a pile foundation in a multi layered soil. Reasonably good agreement is obtained between the present and existing solutions. Compared with the FEM software ABAQUS, the computational speed of BEM is much faster than that of FEM. Applying BEM in practice is convenient. It is pointed out that the exact measurement of elastic modulus is the key factor for applying BEM in geotechnical engineering.  相似文献   

A controller area network based electric vehicle drive system design     

RAN Zhen y  ZHOU Zhi qing  LI Yue  LI Xin  CHEN Fang hui  WU Fu tang 《保鲜与加工》2008,(6):603-609

To solve the key problems of the electric drive system, a controller area network(CAN) based electric drive system for electric vehicles was designed. The system was composed of two parts: a CAN communication module and a motor control module. The newest transceiver (TJA1040) and a stand alone CAN controller were used during the design of the CAN communication module, and anti jamming measures were employed to guarantee communication stability. The fuzzy vector control method and direct speed control were applied to realize highly robust control functions. This system has high compatibility. It can be installed in electric vehicles as an independent module and be extended through CAN Bus.   相似文献   

An algorithm of symbol timing rapid acquisition and tracking for π/4 DQPSK signals     

GAN Ping  SONG Yan yi  YAN Fang  TAN Xiao heng 《保鲜与加工》2008,(6):642-645

Methods of IF difference detection combined with rapid acquisition synchronization and tracking principle by parallel processing methods were proposed in order to quickly achieve synchronous digital capture and tracking in π/4 DQPSK demodulation.Field program grid array (FPGA) technology to achieve rapid detection of synchronous capture and symbol tracking was adopted by using the improved algorithm in parallel with signal acquisition and frequency offset acquisition. This adoption took into account the characteristics of π/4 DQPSK and was in accordance with the detection of rapid acquisition synchronization and the tracking principle. The experimental results show that clock synchronization signals can be restored stably in eight symbol bit, accurately realize bit timing, and possess strong anti interference capability without affecting frequency differences. This proposed algorithm is suited for frequency hopping communication systems with high transmission rates.  相似文献