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31.
Natural infection with two genotypes of Cryptosporidium in red squirrels (Sciurus vulgaris) in Italy
We investigated the genotypes of Cryptosporidium infecting red squirrels (Sciurus vulgaris L.) in two areas of the Western Alps in Italy. Examination of 141 faecal samples from 70 red squirrels revealed oocysts of Cryptosporidium in 17 animals (24.3%). Based on 18S rRNA gene sequencing, two genotypes of Cryptosporidium species were found: 15 squirrels were positive for the Cryptosporidium ferret genotype and 2 for the Cryptosporidium chipmunk genotype I. The occurrence and intensity of Cryptosporidium infection did not differ between the study areas or sex. More than 85% of the positive animals were adults; however no difference was found between Cryptosporidium infection in the juvenile and adult age groups. Oocysts of the Cryptosporidium ferret genotype measured 5.5 +/- 0.3 x 5.2 +/- 0.2 microm (shape index 1.06) and the Cryptosporidium chipmunk genotype I 5.8 +/- 0.3 x 5.4 +/- 0.3 microm (shape index 1.07). Neonatal and adult CD1 and BABL/c mice inoculated with 1 x 10(3) fresh oocysts of both genotypes did not produce detectable infection. 相似文献
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Rhizobium spp. (local isolate) was used to inoculate three bean (Phaseolus vulgaris L.) varieties; Akman 98, G?ynük 98 and Sehirali 90, in a factorial field experiment. The objective of the experiment was to study effect of inoculation on seed yield, protein range, seed weight, non-soaker capacity and hydration index. Rhizobium spp. inoculation and nitrogen fertilizer significantly increased characters tested. This study revealed that inoculation with Rhizobium spp. improved seed quality. 相似文献
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基于大涡模拟动态亚格子湍流模型和三维时均N-S方程,应用非结构网格的滑移网格技术,进行了混流式水轮机全流道动静干扰的三维非定常湍流计算。捕捉到了水轮机各过流部件内的流场随时间的变化情况。计算结果表明水轮机中的水流处于复杂的湍流运动状态,随着时间的推移,水轮机内部湍流运动变化剧烈,得到的计算结果更接近实际流场的分布。 相似文献
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以具体工程小清河济南城区段为例,研究水库型河道在确定主河槽河岸设计高程应考虑的影响因素。提出对于既有景观功能又有防洪要求的河道,主河槽河岸设计高程应分汛期与非汛期进行考虑,综合防洪要求及景观效果确定所需高程。 相似文献
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通过对三相四线制供电系统特点和非线性负载电流谐波污染分析,提出了可同时补偿电压跌落、谐波电流的负载端低压综合电能质量补偿方法,并设计了对应的混合型低压电能质量综合补偿器（UPQC）。通过实验和仿真,结果表明：此补偿方法和混合型低压电能质量综合补偿器能有效的解决非线性负载形成的谐波和无功电流对电网供电质量的影响,是集中式电能综合补偿的必要补充,有一定的发展前景和实用价值。 相似文献
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滴头作为滴灌系统的关键部件，其性能的优劣直接反映了整个滴灌系统寿命、节水性及灌水的均匀性。流道的堵塞问题一直是抑止滴灌技术发展的主要瓶颈，而随着再生水滴灌、微咸水滴灌、雨水滴灌等技术的迅速发展，对滴灌系统抗堵塞能力也提出了更高的要求，堵塞问题成为了滴灌技术研究的热点。分析了滴头堵塞诱发的研究现状，就流道内的堵塞诱发因素、诱发过程、生物膜的存在对于堵塞的影响、流道内生物膜生物、物理特征以及控制方法等几个问题进行了探讨，并结合现代分子生物学技术及测量技术提出了有关流道内生物膜微生态多样性、生长特征等问题研究的发展方向。 相似文献
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Goufo Yemtsa 《中国农村水利水电》2009,(5):108-111
非洲不但有十分丰富的天然气、煤等矿物能源，太阳能和风能等,而且有十分丰富的水电资源，但这些丰富的可再生能源并未得到有效利用。本文通过非洲的电力发展状况和农村电气化发展现状分析，探讨了非洲地区电力发展的障碍，以及非洲农村地区小型水电发展的前景。 相似文献
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Vítovec J Hamadejová K Landová L Kvác M Kvetonová D Sak B 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2006,53(5):239-243
A total of 4338 faecal samples, 135 of sows, 3368 of pre-weaned and 835 of post-weaned piglets from eight farms in South Bohemia, Czech Republic were collected and examined for Cryptosporidium infection. No sow, but 5.7% pre-weaned and 24.1% post-weaned piglets were positive for Cryptosporidium infection. No relationship was found between diarrhoea and Cryptosporidium infection in any of the different age groups (pre- and post-weaned piglets). Four piglets, which were sporadically shedding cryptosporidia in faeces, were necropsied. Neither clinical signs of diarrhoea nor macroscopical changes were found. Histologically, a moderate infection of cryptosporidia was detected in the glandular epithelium along the large intestine, with predisposition to the ansa centralis of the colon. No inflammatory response in the lamina propria was observed. Cryptosporidia were also commonly found in the glandular epithelium of submucosal lymphoglandular complexes in the colon. Cryptosporidium isolates from all farms were identified as Cryptosporidium suis using molecular markers (SSU rRNA). All of the C. suis strains obtained were larger [6.2 (6.0-6.8) x 5.5 (5.3-5.7) microm] than any isolate described so far [4.6 (4.4-4.9) x 4.2 (4.0-4.3) microm] and did not appear to be infective for neonatal BALB/c mice. 相似文献