萝卜离体再生的影响因素   总被引：5，自引：0，他引：5  

武剑  龚义勤  邓波  魏跃  任同辉 《中国蔬菜》2003,1(6):6-8

对影响萝卜离体再生的因素进行了研究,结果表明,不同基困型材料均以带柄子叶的再生频率最高;不同基因型材料所需的最适激素浓度和配比不同,在一定范围内提高6-BA浓度可促进不定芽的分化;苯基脲类细胞分裂素TDZ与嘌呤型细胞分裂素6-BA和KT相比,对促进萝卜不定芽分化的效果更好;AgNO_3可显著提高不定芽再生频率;NAA诱导生根的效果优于IAA。  相似文献   

按不同氨基酸模式配制肉鸭日粮的比较研究     

何健  冯光德 《中国家禽》2004,26(4):13-15

试验比较了不同氨基酸模式对肉鸭生产性能的影响。将320只1日龄的樱桃谷鸭随机分为4组,每组80只,设4个重复,每个重复20只,公母各半,分别饲喂四种日粮(按四种氨基酸模式配制),试验期21天。结果表明:日粮的氨基酸模式为Lys∶Met∶Trp∶Thr=100∶45∶21∶62时,其2周和3周体重高于其它各组,但差异不显著(P>0.05),且F/G和血清尿酸水平最低。  相似文献   

徐州地区烟粉虱发生规律及治理措施初探   总被引：4，自引：0，他引：4  

戴率善  刘宗泉  杜予州  周福才  张贵永  高素兰  仇保华  李建树  李艳红 《中国植保导刊》2004,24(3):17-20

通过近2a观察表明,徐州地区烟粉虱在田间消长大致分4个阶段,成虫有4个主要迁移期;其喜干热、耐高湿的生活习性、冬季保护地栽培面积的扩大、外来虫源的传入及防治不力是烟粉虱种群数量累积并引起暴发的主要原因;主要寄主有9科20余种栽培作物;寄主植株上虫量垂直分布:棉花上部>中部>下部,温室蔬菜一般中、下部>上部。可采取严格检疫、清洁田园、轮作换茬、黄板诱杀、覆盖防虫网、保护和利用天敌、药剂防治等措施进行治理。  相似文献   

西安白鹿塬约85OO年来土壤侵蚀研究     

杜娟  赵景波 《干旱区资源与环境》2004,18(2):136-140

本文根据作者的观察和测量，对西安市白鹿塬塬区的土壤侵蚀状况进行了研究。研究表明，白鹿塬塬区确定土壤侵蚀的主要依据是土层厚度的变化，并非侵蚀面。塬区侵蚀是塬边侵蚀较强，向塬里侵蚀逐渐减弱。塬面上高地侵蚀弱，低地侵蚀强，暂时性线状流水长期汇集而成的冲沟对黄土塬区土壤的侵蚀更加强烈。气候对土壤侵蚀也有影响，冷干和温湿气候均会产生土壤侵蚀，冷干期侵蚀量比温湿期大，前者侵蚀厚度比后者大7倍多。  相似文献   

Model establishment and biological behaviour observation of mouse blastocyst co-cultured with hepatocarcinoma cell lines with differently invasive and metastatic potential in vitro     

LI Da-qiang  WANG Zhi-biao  BAI Jin  ZHAO Jie  HU Kai  WANG Yuan  DU Yong-hong 《园艺学报》2004,20(1):32-37

AIM: To explore interaction and biological behaviour changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvironment. METHODS:The models of mouse blastocysts co-cultured with human hepatocarcinoma cell lines were established, then biological behaviours and mutual effects of the two kinds of cells in co-culture system were observed. RESULTS: Compared with control group, hepatocarcinoma cells with differently invasive and metastatic potential significantly enhanced the rates of blastocyst hatchment , attachment and outgrowth(P＜0.05). There was no significant difference in those among hepatocarcinoma cells co-cultured groups (P＞0.05). The blastocyst hatched and attached to hepatocarcinoma cells with differently invasive and metastatic potential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells on interface showed changes of growth direction and cell shapes and did not invade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted blastocyst development. Blastocysts implanted and invaded hepatocarcinoma cells with differently invasive and metastatic potential in vitro, which indicate that blastocyst implantation in vitro does not relate with the kinds and differential level of interactional cells and the low selectivity maybe relate with high adaptability of early life.  相似文献   

Antitumor effect of chlorophyllin in vitro     

DING Xiao-wen  ZHENG Shu  PENG Jia-ping  LV Qing-hua  HUANG Jian 《园艺学报》2004,20(9):1659-1663

AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC₅₀ value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G₁ phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibited the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G₁ phase.  相似文献   

专家系统在植保上的应用   总被引：3，自引：0，他引：3  

丁建  白小玲 《江西植保》2004,27(1):40-41,39

专家系统在植保上主要应用于病虫害诊断、预测预报、决策管理等领域。植保专家的特点是应用范围广、实用性增强、新技术和新方法不断应用、有向综合性系统发展的趋势。文中还对植保专家系统存在的问题和发展对策进行了讨论。  相似文献   

Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction     

DU Yi-mei  TANG Ming  LIU Chang-jin  HONG Zhi-gang  KE Qin-mei  DI Jiu-fang  LUO Hong-yan  HU Mou-xian  HU Xin-wu  XI Jiao-ya  TANG Bi  Jurgen Hescheler 《园艺学报》2004,20(9):1537-1541

AIM: To determine the role of Kv1.2, Kv1.5, Kv2.1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1.2/Kv1.5/Kv2.1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells . IKv of PASMC was decreased after 24 h hypoxia, . ② The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on them. ③ The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies and the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1.2, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated HPV.  相似文献   

Induction of tumor-specific cytotoxic T lymphocytes in vitro by dendritic cells pulsed with different glioma antigens     

ZHU Xin-mei  QIAO Jian  XIAO Bao-guo  LU Chuan-zhen 《园艺学报》2004,20(4):584-589

AIM: The goal of this study was to compare different methods for tumor antigen preparation, to observe the induction of tumor-specific cytotoxic T lymphocytes in rats by dendritic cells (DCs) pulsed with different tumor antigens. METHODS: The precursors of dendritic cells were isolated from bone marrow of rats, stimulated in vitro with recombinent rat granulocyte-macrophage colony-stimulating factor (rrGM-CSF) and interleukin-4 (rrIL-4). Then rat DCs were pulsed with C6 tumor cell antigens prepared with different methods: freeze-thaw, boiling or total protein extracted from ultrasonic crushed tumor cell. Subsequently primed DCs were cocultured with T lymphocytes isolated from spleen to induce CTL. Lymphocyte chemoattractant factor from DCs and cytokine IFN-γ release were determined by ELISA, the cytotoxicity of CTL was assayed by JAM test. RESULTS: DCs pulsed with boiled tumor cell in vitro induced an enhanced ability of T-cell proliferation and cytotoxic T lymphocyte activity.CONCLUSION: Our results demonstrated that DCs primed with boiled tumor cell may represent a method for inducing immune responses against the entire repertoire of tumor antigens of malignancies.  相似文献   

中国美利奴多胎细毛羊及杂交后代多胎性状基因型分析   总被引：2，自引：0，他引：2  

王建华  何其宏  张永胜  魏荣安  马春萍  库木斯汗  吴文寿  王宏伟  毛路山  王惠 《中国草食动物》2004,24(4):12-13

首次在国内采用了多胎性状基因型诊断法对多胎性状进行选择。试验采集中国美利奴多胎细毛羊及其与Romilly·Hills杂交后代、横交一代样品数分别为 47份、3 8份、2 6份 ,提取DNA确定多胎主效基因FecB 位点 ,酶切检测纯合基因BB、杂合基因B 和非多胎基因 。通过检测 ,三种基因型占样品数百分比分别为 :多胎细毛羊为 2 5 5 3 %、48 94%、2 5 5 3 % ,基因型与产羔符合率为 85 3 7% ;杂交一代为 0、76 3 2 %、2 3 68% ,基因型与产羔符合率为 76 19% ;横交一代为3 0 77%、5 7 69%、11 5 4%。在多胎性状的选择中 ,要坚决剔除非多胎基因个体 ,以缩短选育时间。  相似文献