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21.
The aim of this study was to examine the effect of the nitrification inhibitor nitrapyrin on the fate and recovery of fertilizer nitrogen (N) and on N mineralization from soil organic sources. Intact soil cores were collected from a grassland field. Diammonium phosphate (DAP) and urea were applied as N sources. Cores were equilibrated at –5 kPa matric potential and incubated at 20 °C for 42 to 56 days. Changes in NH4+‐N, accumulation of NO3‐N, apparent recovery of applied N, and emission of N2O (acetylene was used to block N2O reductase) were examined during the study. A significant increase in NH4+‐N released through mineralization was recorded when nitrapyrin was added to the control soil without N fertilizer application. In the soils to which N was added either as urea or DAP, 50–90 % of the applied N disappeared from the NH4+‐N pool. Some of this N (8–16 %) accumulated as NO3‐N, while a small proportion of N (1 %) escaped as N2O. Addition of nitrapyrin resulted in a decrease and delay of NH4+‐N disappearance, accumulation of much lower soil NO3‐N contents, a substantial reduction in N2O emissions, and a 30–40 % increase in the apparent recovery of added N. The study indicates that N recovery can be increased by using the nitrification inhibitor nitrapyrin in grassland soils at moisture condition close to field capacity.  相似文献   
22.
The prolonged use of vehicular traffic for farming creates subsoil compaction, which reduces crop yield and deteriorates the physical conditions of the soil. Field experiments were conducted during 2002–2003 and 2003–2004 in Pakistan to study subsoil compaction effects on soil bulk density, total porosity, yield and yield components of wheat. Soil compaction was artificially created at the start of the experiment using 7.0 t roller having length of 1.5 m and diameter of 1.22 m. Treatments consisted of T1 = control (no compaction), T2 = two passes of roller, T3 = four passes of roller, T4 = six passes of roller. The experiments were arranged in randomised complete block with four replications. Results indicated that subsoil compaction adversely affected the bulk density, total porosity of soil and root length during both the years. Soil compaction increased the bulk density (BD) from 1.37 for T1 to 1.57, 1.61 and 1.72 Mg m−3 whereas decreased the total porosity from 47.3% for T1 to 40.0, 37.4 and 34.5% for T2, T3 and T4, respectively. Similarly grain yield decreased from 4141.7 for T1 to 3912.8, 3364.5 and 3010.3 kg ha−1 for T2, T3 and T4, respectively. The deteriorating effect of compaction depended upon the degree of compaction. Subsoil compaction adversely affected the yield and yield attributes of wheat during both years of experiments. The subsoil compaction adversely affected soil physical conditions, which substantially decreased the yield of wheat. Therefore, appropriate measures of periodic chiselling, controlled traffic, conservation tillage, and incorporating of crops with deep tap root system in rotation cycle is necessary to minimize the risks of subsoil compaction.  相似文献   
23.

Effect of poultry manure (PM) and four inorganic phosphorus (P) fertilizers sources, i.e., diammonium phosphate (DAP), single super phosphate (SSP), nitrophos (NP) and triple super phosphate (TSP) on crop production and P utilization efficiency (PUE) of maize was studied. Both inorganic P fertilizers and PM applied alone or combined in 50:50 proportions at equivalent rate of 90 kg P2O5 ha?1. Results indicated that inorganic P sources with PM significantly increased plant height, leaf area and chlorophyll content. Average values showed that combined application of inorganic P with PM increased grain yield by 19 and 41% over inorganic P and PM alone, respectively. Similarly, increase in P-uptake due to the combined application of inorganic P + PM was 17% compared to sole inorganic P. Phosphorus utilization efficiency of inorganic P was increased with PM and the highest PUE was recorded in DAP + PM. Generally, combination of DAP + PM proved superior over the remaining P fertilizers.  相似文献   
24.
Strains of Pseudomonas syringae pv. syringae (Pss) were isolated from healthy and diseased stone fruits tissues sampled from 38 stone fruits orchard sites in Iran in 2010 and 2011. These strains were tested for pathogenicity and the presence of the syrB gene and were genetically characterized by using ERIC (enterobacterial repetitive intergenic consensus), REP (repetitive extragenic palindromes), and BOXAIR and IS50 (insertion sequences) primers and PCR. All 78 strains of Pss tested were moderately to highly pathogenic on Loring peach seedlings. A total of 78 isolates of the Pss amplified a 752-bp fragment with the syrB primers. To assess genetic diversity among the strains, genomic DNA was extracted from strains and used in rep-PCR and IS50-PCR analysis. Cluster analysis was performed using UPGMA. The strains of Pss were separated into nine distinguishable genotypic groups by the combination data set of both rep-PCR and IS50-PCR at 73 % similarity level. There was no significant correlation between genetic diversity and geographical origin of the isolates. These results indicate that a combination of rep-PCR and IS50-PCR fingerprinting can be used as a high resolution genomic fingerprinting method for elucidating intrapathovar diversity among strains of Pss. The results of this study demonstrated the existence of a considerable genetic diversity among Pss strains causing canker of stone fruit trees in Iran. In this study, genetic variability in Iranian strains of Pss were established, which will be of immense use in the development of resistant genotypes against this bacterial pathogen.  相似文献   
25.
The mechanism of inhibition of alpha-chymotrypsin by benzoylsalireposide (1), a phenolic glycoside isolated from Symplocos racemosa, was investigated. Lineweaver-Burk and Dixon plots and their secondary replots showed that 1 was a non-competitive inhibitor of this enzyme with the Ki value of 29.60 microM.  相似文献   
26.
We report for the first time the capability of four-leaf clover (Marsilea quadrifolia), a wetland plant which grows rooted in soil, in efficiently treating sewage. The use of M. quadrifolia was made possible because of the special attributes of the SHEFROL® (SHEet Flow ROot Level) bioreactor in which it was employed. This bioreactor enables the use of free-floating aquatic plants as well as terrestrial and rooted-in-soil wetland plants by hydroponics. The plants are staked in narrow channels to enable them to support each other while sewage is made to flow rapidly as a sheet of wastewater at a level that covers only the plant roots (hence the name). It was seen that M. quadrifolia was able to treat sewage of strength varying in the chemical oxygen demand (COD) range of 600–1800 mg/L to the extent of >?80% at a hydraulic retention time (HRT) of just 4.5 h. There was a near total removal of biological oxygen demand and suspended solids while total Kjeldahl nitrogen, soluble phosphorous, and heavy metal zinc were also substantially removed. The macrophyte was equally effective when used indoors under artificial lighting, as well as when used outdoors.  相似文献   
27.
‘Phosphate solubilizing bacteria' (PSBs) are able to release unavailable P from native and applied P sources into plant‐available soil pool through their solubilizing and acidifying effects. The effects of three indigenous and one exotic PSBs on P solubilization from different P sources, plant biomass production, and P‐uptake efficiency of maize (Zea mays L.) were examined in an incubation and greenhouse study. For incubation study, surface (0–15 cm) soil was collected from an arable field (Inceptisols) and amended with rock phosphate (RP), single superphosphate (SSP), poultry manure (PM), and RP+PM with and without PSBs. The amended soil was incubated in the control environment at 25 ± 2°C for a total of a 100‐d period to establish relative potential rate of P solubilization of added P sources. A complementary greenhouse experiment was conducted in pots by growing maize as a test crop. Growth characteristics, P‐uptake, and P‐utilization efficiency (PUE) were determined. Phosphate solubilizing bacteria generated a solubilization effect on different P sources by releasing more P into plant‐available soil pool, i.e., 14.0–18.3 µg g?1 in RP, 5.0–9.9 µg g?1 in SSP, 1.4–4.4 µg g?1 in PM, and 4.5–7.8 µg g?1 in RP+PM compared to their sole application without PSBs. The available P from inorganic SSP declined continuously from the mineral pool (after day 30) and at the end 40% of applied P was unaccounted for. However, P losses were reduced to 28 and 27% when PSBs (PSB1 and PSB3) were applied with superphosphate treatments. In the absence of PSBs, the recoveries of applied P (in soil) from RP, SSP, PM and RP+PM were 4, 25, 9, and 12%, respectively, those had been increased to 14, 30, 12 and 15% in the presence of PSBs. Similarly, the plant biomass in RP+PSBs treatments compared to the RP without PSBs increased between 12–30% in first sampling (30 DAG) and 13–30% in the second sampling (60 DAG). The P utilization efficiency (PUE) in plants supplemented with PSBs was 20–73% higher compared to those without PSBs. The detection of oxalic and gluconic acids in culture medium treated with PSBs (7.8–25.0 and 25–90 mg L?1, respectively) confirmed the production of organic acids by the indigenous bacterial isolates. This study indicate that low P recovery both in plant and soil can likely be improved by using indigenous PSBs and organic amendment poultry manure, which allowed a more efficient capture of P released due to P solubilization.  相似文献   
28.
Thermography is proposed to be an alternative non-destructive and rapid technique for the study and diagnosing of salt tolerance in plants. In a pot experiment, 30 cultivars of wheat (Triticum aestivum L.) were evaluated in terms of their leaf temperature and shoot growth and their ion distribution responses to NaCl salinity at two concentration levels: the control with electrical conductivity (EC) of 1 dS m?1 and salinity treatment with EC of 16 dS m?1 (150 mM). A completely randomized block design with factorial treatments was employed with three replications. The results indicated that thermography may accurately reflect the physiological status of salt-stressed wheat plants. The salt stress-based increase in leaf temperature of wheat cultivars grown at 150 mM NaCl reached 1.34°C compared to the control. According to the results obtained, it appears that thermography has the capability of discerning differences of salinity tolerance between the cultivars. Three salt-tolerant wheat cultivars, namely Roshan, Kharchia and Sholeh, had higher mean shoot dry matter (0.039 g plant?1) and higher mean ratio of leaf K+/Na+ (14.06) and showed lower increase in the mean leaf temperature (0.37°C) by thermography compared to the control. This was while nine salt-sensitive cultivars, namely Kavir, Ghods, Atrak, Parsi, Bahar, Pishtaz, Falat, Gaspard and Tajan, had lower mean plant dry matter production (0.027 g plant?1), lower mean ratio of K+/Na+ (9.49) and higher mean increases in leaf temperature (1.24°C).  相似文献   
29.
The heterogeneity of CD4+ T cells has been investigated since the late 1970s, when their Th1 and Th2 subsets were coined. Later studies on the cutaneous form of the Leishmaniasis were focused on the experimental models of Leishmania major infection using the susceptible BALB/c and the resistant C57BL/6 mice. At the early 21st century, the Treg subpopulation was introduced and its role in concomitant immunity, responsible for lifelong resistance of the host to the reinfection was proposed. Subsequent studies, mainly focused on the visceral form of the infection pointed to the role of IL-17, produced by Th17 subset of CD4+ T cells that along the neutrophils were shown to have important yet equivocal functions in protection against or exacerbation of the infection. Altogether, the current knowledge indicates that the above four subsets could orchestrate the immune, the regulatory and the inflammatory responses of the host against different forms of leishmaniases. Key Words: Immunity, Leishmaniasis, T-Lymphocytes  相似文献   
30.
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