Differentiation of infectious bursal disease viruses by restriction enzyme analysis of RT-PCR amplified VP1 gene sequence     

Tiwari AK  Kataria RS  Indervesh  Prasad N  Gupta R 《Comparative immunology, microbiology and infectious diseases》2003,26(1):47-53

In order to differentiate infectious bursal disease virus (IBDV) isolates/strains, a quick method of RT-PCR followed by restriction enzyme analysis of VP1 gene sequence is being reported for the first time. A 480 bp fragment, comprising one of the RNA dependent RNA polymerase motifs of VP1 gene sequence of an Indian classical virus, an attenuated vaccine strain, Georgia and two Indian field isolates, genetically similar to reported very virulent strains of IBDV, was amplified by RT-PCR. Restriction enzyme digestion of PCR products with Taq1 enzyme generated distinct profile for field isolates, different from the classical and attenuated viruses, whereas restriction profile with BstNI restriction enzyme was similar in all the viruses, irrespective of the pathotype. Therefore, the present results suggest that Taq1 digestion can be taken up for the differentiation of field isolates from the classical and vaccine strains. The sequence analysis of VPI gene of reported very virulent IBD viruses from Europe and Japan, using 'MapDraw' programme of Lasergene software, revealed similar restriction enzyme profile as in Indian field isolates.  相似文献   

Canine brucellosis in a dog imported into the UK     

Dunne J  Sehgal K  McMillan A  Perret L 《The Veterinary record》2002,151(8):247

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Turkey herpesvirus and Marek's disease virus. A comparative appraisal.     

L B Prasad 《Comparative immunology, microbiology and infectious diseases》1979,2(2-3):335-358

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Efficacy of disinfectants and hand sanitizers against avian respiratory viruses     

Patnayak DP  Prasad AM  Malik YS  Ramakrishnan MA  Goyal SM 《Avian diseases》2008,52(2):199-202

Disinfectants play a major role in the control of animal diseases by decontaminating the farm environment. We evaluated the virucidal efficacy of nine commonly used disinfectants on a nonporous surface contaminated experimentally with avian metapneumovirus (aMPV), avian influenza virus, or Newcastle disease virus (NDV). Phenolic compounds and glutaraldehyde were found to be the most effective against all three viruses. Quaternary ammonium compounds were effective against aMPV but not against the other two viruses. In addition, efficacy of commercially available hand sanitizers was evaluated on human fingers contaminated with aMPV and NDV. All three hand sanitizers tested were found to be effective against both viruses within 1 min of application on fingers.  相似文献   

Influence of Pre-Drying Treatments on Physico-Chemical and Phytochemical Potential of Dried mahua Flowers     

Pinakin  Dave Jaydeep  Kumar  Vikas  Kumar  Satish  Kaur  Sawinder  Prasad  Rasane  Sharma  Basista Rabina 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):576-582

Plant Foods for Human Nutrition - Mahua (Madhuca longifolia) flowers are boon for the local tribes of India and have been successfully utilized traditionally for the value addition, and are very...  相似文献   

硼酸处理对Sensatiou芒果成熟的影响          下载免费PDF全文

曾燕如  SrivastavaGC  PandeyM  PrasadNK. 《浙江农林大学学报》1996,13(3):263-269

用10mmolL-1 硼酸处理Sensatinn芒果果实,日的在于了解硼酸时延缓成熟过程,尤其对延缓那些与成熟有关的生理变化是否有一定的作用。8d的成熟过程所观浏的结果表明.①硼酸处理的鲜果质量下降不如对照明显,果皮中叶绿素含量的变化也类似,②硼酸处理的果实中,果胶甲脂酶及多聚半乳糖醛酸酶的活性高于时照,且果胶甲酷酶的活性比多聚半乳糖醛酸酶的活性早2d达到峰值,⑧与对照相比较,硼酸能降低果实的呼吸作用,而时果肉的总糖含量没有影响。  相似文献   

Genetic dissection of root architectural traits by QTL and genome‐wide association mapping in rapeseed (Brassica napus)     

Aysha Kiran  Abdul Wakeel  Rod Snowdon  Wolfgang Friedt 《Plant Breeding》2019,138(2):184-192

Variation in root architecture is essential for the adaptation of plants to target environments. A non‐destructive gel‐based mini‐rhizotron system was used for root architecture trait phenotyping. This system has facilitated the visualization of root architectural traits in large genotype collection of rapeseed including 94 double haploid (DH) lines from “Express617” x “V8” and 439 inbred lines (ASSYST diversity set). A high‐density Express617‐V8 linkage map was used for quantitative trait loci (QTLs) identification in DH population based on standard composite interval mapping. 6K SNPs were analysed for association mapping of root traits in ASSYST diversity set. A large variation, broad segregation and medium–low heritability of root architectural traits, primary root length and growth rate, lateral root number, lateral root length and lateral root density, were observed. In the double haploid population, 11 QTL regions, and in the diversity set, 38 significant marker–trait associations were detected. Significant marker–trait associations proved that these are quantitatively inherited traits controlled by multiple genes which revealed to proceed for genetic improvement and selection of rapeseed lines with improved root system.  相似文献   

Differential Expression Pattern Of MADS Box Genes in Floral Whorls of Garcinia indica     

Patil  Reshma V.  Pawar  Kiran D. 《Journal of Crop Science and Biotechnology》2019,22(4):363-369

Journal of Crop Science and Biotechnology - Flower formation is one of the main developmental stages in the life cycle of flowering plants that can be used as a model system to elucidate the...  相似文献   

Muscle biochemical constituents of cultured amberjack <Emphasis Type="Italic">Seriola dumerili</Emphasis> and their influence on raw meat texture     

Dhirendra Prasad Thakur  Katsuji Morioka  Naoya Itoh  Mizuho Wada  Yoshiaki Itoh 《Fisheries Science》2009,75(6):1489-1498

The study was conducted to evaluate the meat texture, muscle proximate composition, lipid class composition, and collagen content of cultured amberjack and to compare these parameters with those of the yellowtail. Our results showed that the meat texture of cultured amberjack was tougher and had a lower degree of seasonality than that of cultured yellowtail. Muscle lipid and collagen content also varied in the two fish species over the study period. Meat breaking strength was not correlated with any of the muscle constituents, indicating that variations in the meat texture of cultured amberjack was not directly influenced by the changes in the muscle biochemical constituents.  相似文献   

The use of piperonyl butoxide and MGK-264 to improve the efficacy of some plant-derived molluscicides     

Kiran Singh  Ajay Singh  D. K. Singh 《Pest management science》1998,54(2):145-149

Synergism of an oil of Azadirachta indica, a powdered extract of Allium sativum bulbs and an oleoresin of Zingiber officinale rhizomes by piperonyl butoxide and MGK-264 was studied against the snails Lymnaea acuminata and Indoplanorbis exustus. The active components of these plant-derived molluscicides, respectively azadirachtin, allicin and [6]gingerol, were also combined with these synergists. Both piperonyl butoxide and MGK-264 enhanced the toxicity of all of the test compounds. The response of snails to the synergised mixtures was both time- and dose-dependent. © 1998 Society of Chemical Industry  相似文献