A gain-of-function polymorphism controlling complex traits and fitness in nature     

KV Prasad  BH Song  C Olson-Manning  JT Anderson  CR Lee  ME Schranz  AJ Windsor  MJ Clauss  AJ Manzaneda  I Naqvi  M Reichelt  J Gershenzon  SG Rupasinghe  MA Schuler  T Mitchell-Olds 《Science (New York, N.Y.)》2012,337(6098):1081-1084

Identification of the causal genes that control complex trait variation remains challenging, limiting our appreciation of the evolutionary processes that influence polymorphisms in nature. We cloned a quantitative trait locus that controls plant defensive chemistry, damage by insect herbivores, survival, and reproduction in the natural environments where this polymorphism evolved. These ecological effects are driven by duplications in the BCMA (branched-chain methionine allocation) loci controlling this variation and by two selectively favored amino acid changes in the glucosinolate-biosynthetic cytochrome P450 proteins that they encode. These changes cause a gain of novel enzyme function, modulated by allelic differences in catalytic rate and gene copy number. Ecological interactions in diverse environments likely contribute to the widespread polymorphism of this biochemical function.  相似文献   

Feedback regulation of transcriptional termination by the mammalian circadian clock PERIOD complex   总被引：1，自引：0，他引：1  

K Padmanabhan  MS Robles  T Westerling  CJ Weitz 《Science (New York, N.Y.)》2012,337(6094):599-602

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GnRH-1 mRNA, LH surges, steroid hormones, egg production, and intersequence pause days alter in birds exposed to longer wavelength of light in the later stages of production in Gallus gallus domesticus     

Reddy IJ  David CG  Selvaraju S  Mondal S  Ravi Kiran G 《Tropical animal health and production》2012,44(6):1311-1317

The objective of this was to establish the effects of red spectrum of light (650 nm, treated n = 12) and normal spectrum of light (450 nm control = 12) on GnRH-I mRNA expression, amplitude and frequency of luteinizing hormone (LH), and egg production from 72-82 weeks of age in white leghorn hens. Birds exposed to red spectrum of wavelength significantly improved (P < 0.01) steroid hormone, and egg production improved over old laying 72 to 82 weeks. Weekly interval profiles followed the same pattern. At 77th weeks of age blood, samples from both the groups were collected at every 3 h for 36 h to study the pulsatile secretion of LH surges. Plasma LH concentration was higher (P < 0.01) in treated birds with more number of frequencies and amplitude LH surges in plasma of treated birds. LH frequencies were more pronounced and advanced during 36 h of sampling at 3 h interval in treated birds. Weekly interval of plasma LH, E2β, and P(4) concentrations increased (P < 0.01) in treated birds from 72 to 82 weeks of age. GnRH-I mRNA concentration was significantly (P < 0.01) higher in birds exposed to red spectrum of light compared to controls. It is hypothesized that exposure of birds to red spectrum of light-enhanced (P < 0.01) GnRH-I mRNA with more number of yellow yolky follicles was found in birds exposed to red spectrum of light during 77 days (72-82 weeks of age) of experimental period. It is concluded that higher levels of GnRH-I mRNA, LH, E2β, and P(4) concentration with lower incidence of pause days enabled the birds to lay more eggs even later in the productive period by modulating the wavelengths of light under normal husbandry conditions.  相似文献   

Development and utilization of novel SSRs in foxtail millet [Setaria italica (L.) P. Beauv.]     

Sarika Gupta  Kajal Kumari  Mehanathan Muthamilarasan  Alagesan Subramanian  Manoj Prasad 《Plant Breeding》2013,132(4):367-374

Although the foxtail millet [Setaria italica (L.) P. Beauv.] is recently regarded as a model crop for studying functional genomics of biofuel grasses, its genetic improvement to some extent was limited due to the non‐availability of molecular markers, particularly the microsatellite markers and the saturated genetic linkage map. Considering this, we attempted to generate a significant number of microsatellite markers in cultivar ‘Prasad’. Two hundred and fifty‐six clones were sequenced to generate 41.82‐kb high‐quality sequences retrieved from genomic library enriched with dinucleotide repeat motifs. Microsatellites were identified in 194 (76%) of the 256 positive clones, and 64 primer pairs (pp) were successfully designed from 95 (49%) unique SSR‐containing clones. The 67.4% primer designing ability, 100% PCR amplification efficiency and 45.3% polymorphic potential in the parents of F₂ mapping population established the efficacy of genomic microsatellites. All the 64 microsatellite markers displayed high level of cross‐species amplification (~67%) in 10 millets and non‐millets species. These experimental findings suggest the utility and efficacy of SSRs in diverse genotyping applications, resolving QTLs, phylogenetic relationships and transferability in several important grass species.  相似文献   

Prevalence and genotypic (G and P) determination of porcine group A rotaviruses from different regions of India     

Zunjar Baburao Dubal  Kiran N. Bhilegaonkar  Sukhadeo B. Barbuddhe  Rahul P. Kolhe  Simranpreet Kaur  Shriya Rawat  Prejit Nambiar  Muthu Karunakaran 《Tropical animal health and production》2013,45(2):609-615

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Effect of crop‐residue management on the production and agronomic nitrogen efficiency in a rice–wheat cropping system     

S. N. Sharma  Rajendra Prasad 《植物养料与土壤学杂志》2008,171(2):295-302

The rice–wheat cropping system (RWCS), producing about 5–10 Mg ha^–1 y^–1 of grain, is the backbone of food‐crop production in South‐East Asia. However, this system shows signs of fatigue as indicated by declining yields, negative nitrogen (N) balances, and reduced responses to applied fertilizer at some research centers. The return of rice and wheat residues can recycle up to 20%–30% of the N absorbed by the crops. However, their wide C : N ratio can temporarily immobilize native and applied N. To overcome this immobilization, wheat‐straw application was supplemented with the incorporation of Sesbania green manure and mungbean residues, and their effects on productivity, agronomic N efficiency, and system's apparent N balances were studied. Combining the application of wheat straw with Sesbania green manure or mungbean residues increased cereal grain yield and agronomic N efficiency and improved the generally negative apparent N balances. The combined use of wheat straw and mungbean produced an additional 0.5–0.6 t ha^–1 protein‐rich grain and thus appears to be the most promising residue‐management option for rice–wheat cropping systems in South Asia, provided that the transition cropping season between wheat harvest and rice transplanting is long enough.  相似文献   

Design of Optimum Response Surface Experiments for Electro-Coagulation of Distillery Spent Wash     

R. Krishna Prasad  R. Ram Kumar  S. N. Srivastava 《Water, air, and soil pollution》2008,191(1-4):5-13

Electro-coagulation (EC) of a distillery spent wash has been optimized by Box–Behnken design of surface response analysis in terms of color removal. The design was employed to derive a statistical model for the effect of parameters studied on removal of color using iron anodes. The current density (31 mA/cm²), dilution (17.5%) and time of electrolysis (4 hours) had been found to be the optimum conditions for maximum 95% color removal. The actual color removal at optimized conditions was found to be 93.5% which confirms close to the predicted response using response surface methodology.  相似文献   

Thresholds,sensitive stages and genetic variability of finger millet to high temperature stress          下载免费PDF全文

R. A. Opole  P. V. V. Prasad  M. Djanaguiraman  K. Vimala  M. B. Kirkham  H. D. Upadhyaya 《Journal of Agronomy and Crop Science》2018,204(5):477-492

Finger millet [Eleusine coracana (L.) Gaertn.] is an important coarse cereal crop grown in the arid and semi‐arid regions and often experiences high temperature (HT) stress. The objectives of this research were (i) to quantify effects of season‐long HT stress on physiological and yield traits, (ii) to identify the developmental stages most sensitive to HT stress and (iii) to quantify the genetic variability for HT stress tolerance in finger millet. Research was conducted in controlled environment conditions. HT stress decreased the chlorophyll index, photosystem II activity, grain yield and harvest index. Maximum decrease in number of seeds per panicle and grain yield per plant was observed when stress was imposed during booting, panicle emergence or flowering stages. Maximum genotypic variation was explained by panicle width and number of seeds per panicle at optimum temperature (OT) and grain yield per plant at HT and number of seeds at HT. Based on the stress response and grain yield, tolerant or susceptible genotypes were identified. Finger millet is sensitive to HT stress during reproductive stages, and there was genotypic variability among the finger millet genotypes for number of seeds per panicle and grain yield under HT, which can be exploited to enhance stress tolerance.  相似文献   

Modeling tsunami damage in Aceh: a reply     

Louis R. Iverson  Anantha M. Prasad 《Landscape Ecology》2008,23(1):7-10

In reply to the critique of Baird and Kerr, we emphasize that our model is a generalized vulnerability model, built from easily acquired data from anywhere in the world, to identify areas with probable susceptibility to large tsunamis—and discuss their other criticisms in detail. We also show that a rejection of the role of trees in helping protect vulnerable areas is not justified in light of existing evidence.  相似文献   

A multistage decision support framework to guide tree species management under climate change via habitat suitability and colonization models,and a knowledge-based scoring system     

Anantha M. Prasad  Louis R. Iverson  Stephen N. Matthews  Matthew P. Peters 《Landscape Ecology》2016,31(9):2187-2204

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